BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,622,662	A→G	5.3%	Y3Y (TAT→TAC)	USA300HOU_RS13520 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,622,662	0	A	G	5.3%	352.2 / 7.1	113	Y3Y (TAT→TAC)	USA300HOU_RS13520	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (50/57); new base G (4/2); total (54/59)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 119 total aligned reads displayed.

GATAATTTTAAAGATGATTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATA  >  USA300TCH1516_ALE/2622525‑2622802

aaTAATTTTAAATATGATTGGATTGTAAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATac                                                                                                                                            <  2:758549/140‑1 (MQ=255)

  tAATTTTAAAGATGATTGGTTTGTCAATTCAACAGTAAACCAAATTGTTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATacaa                                                                                                                                          >  3:371922/1‑141 (MQ=255)

    aTTTTAAAGATGATTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATacaaca                                                                                                                                        <  3:415038/141‑1 (MQ=255)

        tAAAGATGATTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATAtt                                                                                                                                    >  3:255781/1‑141 (MQ=255)

        tAAAGATGATTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATAtt                                                                                                                                    <  3:392864/141‑1 (MQ=255)

         aaaGATGATTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATAttt                                                                                                                                   <  2:1005660/141‑1 (MQ=255)

           aGATGATTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTAt                                                                                                                                 >  1:271678/1‑141 (MQ=255)

           aGATGATTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTAt                                                                                                                                 >  1:500366/1‑141 (MQ=255)

            gatgatTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACACAACGACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATc                                                                                                                                <  1:565473/141‑1 (MQ=255)

              tgatTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCAc                                                                                                                              >  3:174025/1‑141 (MQ=255)

               gatTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAGCAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAGTACAACATATTTATCAcc                                                                                                                             >  3:433187/1‑141 (MQ=255)

               gatTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCAcc                                                                                                                             >  1:406134/1‑141 (MQ=255)

                    gTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCa                                                                                                                        <  2:420490/141‑1 (MQ=255)

                    gTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCa                                                                                                                        >  2:347694/1‑141 (MQ=255)

                    gTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTACGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCa                                                                                                                        <  4:244119/141‑1 (MQ=255)

                     tttGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCAt                                                                                                                       >  4:333349/1‑141 (MQ=255)

                       tGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAATACTTCTAAATACAACATATTTATCACCTCTCATaa                                                                                                                     >  4:44803/1‑141 (MQ=255)

                          cAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCACTCATaaaaa                                                                                                                  >  1:1077455/1‑141 (MQ=255)

                             ttCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAaataa                                                                                                               >  1:670769/1‑141 (MQ=255)

                                aaCAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTg                                                                                                            <  1:335535/141‑1 (MQ=255)

                                  cAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGaa                                                                                                          <  1:462026/141‑1 (MQ=255)

                                  cAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGaa                                                                                                          <  2:807402/141‑1 (MQ=255)

                                  cAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGaa                                                                                                          >  2:297464/1‑141 (MQ=255)

                                  cAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCCCTCATAAAAATAAtttca                                                                                                          >  4:265293/1‑138 (MQ=255)

                                    gTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTTTTATCTTCTAAATACAACATATTTATTACCTCTTATAAAAATAATTTAAta                                                                                                        >  2:749048/1‑140 (MQ=255)

                                     tAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGc                                                                                                       <  1:540153/141‑1 (MQ=255)

                                     tAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGc                                                                                                       <  3:229136/141‑1 (MQ=255)

                                     tAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGc                                                                                                       >  1:816343/1‑141 (MQ=255)

                                           aaaTTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATccac                                                                                                 <  1:319598/141‑1 (MQ=255)

                                           aaaTTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATccac                                                                                                 <  1:98477/141‑1 (MQ=255)

                                            aaTTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATccacc                                                                                                >  1:1015212/1‑141 (MQ=255)

                                             attattTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATccacca                                                                                               <  1:241445/141‑1 (MQ=255)

                                               tattTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑c                                                                                            >  3:433664/1‑141 (MQ=255)

                                               tattTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑c                                                                                            <  2:579396/141‑1 (MQ=255)

                                                 tttGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑Ctt                                                                                          <  1:112257/141‑1 (MQ=255)

                                                     tAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑Ctttttt                                                                                      >  1:694734/1‑141 (MQ=255)

                                                         gAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGAc                                                                                  >  2:703628/1‑141 (MQ=255)

                                                           aaaCAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACct                                                                                <  4:45281/141‑1 (MQ=255)

                                                            aaCAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACctt                                                                               <  1:533085/141‑1 (MQ=255)

                                                            aaCAGCACAAAACAACTCCGACGTAAGAAACTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACctt                                                                               >  1:201454/1‑141 (MQ=255)

                                                                gCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCttcttc                                                                           >  2:396784/1‑141 (MQ=255)

                                                                gCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCttcttc                                                                           <  3:176196/141‑1 (MQ=255)

                                                                gCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCttcttc                                                                           <  2:371064/141‑1 (MQ=255)

                                                                 cacaAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTtcttct                                                                          <  4:272447/141‑1 (MQ=255)

                                                                  acaAAGCAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAGTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCCTTTTTTAGACCTtcttct                                                                          <  1:84166/141‑1 (MQ=255)

                                                                  acaAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTa                                                                         >  4:448678/1‑141 (MQ=255)

                                                                  acaAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTa                                                                         <  2:318480/141‑1 (MQ=255)

                                                                  acaAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTCAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTa                                                                         >  2:843895/1‑141 (MQ=255)

                                                                     aaaCAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAAc                                                                      >  3:329877/1‑141 (MQ=255)

                                                                      agcaacTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAGTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCCTTTTTTAGACCTTCTTCTAAAc                                                                      >  1:440213/3‑141 (MQ=255)

                                                                       acaacTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAActc                                                                    >  3:194889/1‑141 (MQ=255)

                                                                          acTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAActctct                                                                 >  2:10433/1‑141 (MQ=255)

                                                                          acTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAActctct                                                                 >  2:81073/1‑141 (MQ=255)

                                                                          acTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAActctct                                                                 <  1:208387/141‑1 (MQ=255)

                                                                            tCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCttt                                                               >  3:379079/1‑141 (MQ=255)

                                                                                 cGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCa                                                          >  1:808307/1‑141 (MQ=255)

                                                                                    aaGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAg                                                       <  2:680749/141‑1 (MQ=255)

                                                                                      aaaaTTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAgcg                                                     <  1:569508/140‑1 (MQ=255)

                                                                                       aaaTTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAgcgc                                                    <  2:408240/141‑1 (MQ=255)

                                                                                       aaaTTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAgcgc                                                    <  1:683980/141‑1 (MQ=255)

                                                                                       aaaTTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATAGTTATCACCTCTCATAAGAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAgcgc                                                    <  2:459379/141‑1 (MQ=255)

                                                                                        aaTTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCa                                                   >  4:16900/1‑141 (MQ=255)

                                                                                          ttACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAGTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCaa                                                  >  1:124994/1‑141 (MQ=255)

                                                                                          ttACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCaat                                                 <  1:297852/141‑1 (MQ=255)

                                                                                          ttACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCaat                                                 <  1:946265/141‑1 (MQ=255)

                                                                                          ttACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCaat                                                 <  1:324452/141‑1 (MQ=255)

                                                                                               aatcaatAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCaattaatt                                            >  1:79376/1‑141 (MQ=255)

                                                                                                 tcaatAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCt                                          <  2:592932/141‑1 (MQ=255)

                                                                                                   aataaataTGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTaa                                        <  4:263897/141‑1 (MQ=255)

                                                                                                     taaataTGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAata                                      <  1:746963/141‑1 (MQ=255)

                                                                                                       aataTGTAAAGTTTCTATTTTTCAAAACTTCTAAGTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAataa                                     >  1:740635/1‑140 (MQ=255)

                                                                                                       aataTGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAAtata                                    <  2:591771/141‑1 (MQ=255)

                                                                                                       aataTGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAAtata                                    >  2:453281/1‑141 (MQ=255)

                                                                                                         tatGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAAt                                  >  3:16824/1‑141 (MQ=255)

                                                                                                          atGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAAtt                                 <  1:437884/141‑1 (MQ=255)

                                                                                                             tAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCTACCAC‑CTATCCTCGACATTCTTCTACACTCTCTTTATCAAAAACGCCATTAATTCTAATATAATTTaa                              >  2:672031/1‑140 (MQ=255)

                                                                                                             tAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAg                              >  2:872120/1‑141 (MQ=255)

                                                                                                             tAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAg                              <  1:703628/141‑1 (MQ=255)

                                                                                                             tAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAg                              <  1:87416/141‑1 (MQ=255)

                                                                                                                    tCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTa                       <  1:966559/141‑1 (MQ=255)

                                                                                                                      tATTTTTCAAAACTTCTAAGTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATAAAATTAAGTCAGTTaa                      <  1:7775/141‑1 (MQ=255)

                                                                                                                      tATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTaaa                     <  1:438497/141‑1 (MQ=255)

                                                                                                                      tATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTaaa                     >  1:471751/1‑141 (MQ=255)

                                                                                                                      tATTTTTCAAAACTTCTAAATACAACATATTTATCACCGCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATAAACTTTAGTCAGTTaaa                     >  2:236559/1‑141 (MQ=255)

                                                                                                                       aTTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTTTTTTTTTTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAat                    >  2:402296/1‑141 (MQ=255)

                                                                                                                        tttttCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGCCATTTAGGTGAACTCTCTTTATCCAAAGCGCACTTAATTCTAATATAATTTAGTCATTTAAata                   >  1:836145/1‑141 (MQ=255)

                                                                                                                        tttttCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAata                   >  1:176987/1‑141 (MQ=255)

                                                                                                                          tttCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATc                 <  1:599164/141‑1 (MQ=255)

                                                                                                                          tttCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATc                 <  2:694734/141‑1 (MQ=255)

                                                                                                                             cAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAat              <  1:317751/141‑1 (MQ=255)

                                                                                                                               aaaCTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAatta            >  4:422673/1‑141 (MQ=255)

                                                                                                                                aaCTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAattat           >  1:72357/1‑141 (MQ=255)

                                                                                                                                aaCTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAattat           >  1:72368/1‑141 (MQ=255)

                                                                                                                                 aCTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAattatt          >  1:1079574/1‑141 (MQ=255)

                                                                                                                                     cTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGa      <  2:68913/141‑1 (MQ=255)

                                                                                                                                       aaaTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGaaa    <  3:387944/141‑1 (MQ=255)

                                                                                                                                       aaaTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGaaa    >  3:308510/1‑141 (MQ=255)

                                                                                                                                       aaaTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCAACAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGaaa    >  3:343658/1‑141 (MQ=255)

                                                                                                                                         aTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAata  >  2:808325/1‑141 (MQ=255)

                                                                                                                                         aTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAata  >  1:1029786/1‑141 (MQ=255)

GATAATTTTAAAGATGATTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATA  >  USA300TCH1516_ALE/2622525‑2622802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`