| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 168,354 | C→G | 5.1% | intergenic (+321/+27) | yfkN_1 → / ← USA300TCH1516_00148 | Trifunctional nucleotide phosphoesterase protein YfkN/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 168,354 | 0 | C | G | 5.1% | 383.4 / 11.3 | 119 | intergenic (+321/+27) | yfkN_1/USA300TCH1516_00148 | Trifunctional nucleotide phosphoesterase protein YfkN/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (53/59); new base G (4/2); total (58/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
TGATGAAACATTTTTGAATCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGACATTTGTTCTCCAGTATT > USA300TCH1516_ALE/168218‑168494 | tgatgaAACATTTTTGAATCATCTAGCAGGTATTAATTTAAAACTCTTTCAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGAtcatca > 1:926710/1‑141 (MQ=255)tgatgaAACATTTTTGAATCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGAtcatca < 2:120864/141‑1 (MQ=255) atgaAACATTTTTGAATCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCAtt < 2:925671/141‑1 (MQ=255) atgaAACATTTTTGAATCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCAtt < 3:119138/141‑1 (MQ=255) atgaAACATTTTTGAATCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCAtt < 1:567964/141‑1 (MQ=255) atgaAACATTTTTGAATCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCAtt < 3:77881/141‑1 (MQ=255) atgaAACATTTTTGAATCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCAtt > 1:288793/1‑141 (MQ=255) tgaAACATTTTTGAATCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCAttt > 1:30334/1‑141 (MQ=255) tgaAACATTTTTGAATCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAACTGAAATGATGATCtttt < 4:32037/141‑1 (MQ=255) aTTTTTGAATCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTc < 2:400679/141‑1 (MQ=255) tttttGAATCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCg < 1:107647/141‑1 (MQ=255) ttttGAATCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAATGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGa > 2:63602/1‑141 (MQ=255) ttttGAATCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGa > 4:436045/1‑141 (MQ=255) tggAATCATCTAGCAGGTATTAATTTAAAACTCTTGAAGGATTATGTTAAGGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATGTTTAAATCTCGAGCTGAAAAGATCATCATTTCAGTTCGAgg < 2:99876/139‑1 (MQ=255) tGAATCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGt > 1:165343/1‑141 (MQ=255) tGAATCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGAAAATAATTTCAGTTCGAgga > 3:195695/1‑140 (MQ=255) gAATCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGtt > 2:1044873/1‑141 (MQ=255) gAATCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGtt > 2:177582/1‑141 (MQ=255) atttatcTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGttt > 4:124223/5‑141 (MQ=255) aaTCATCTAGCAGGTATTAATTTAAAACTCTTTAATGATTATGTTAATGAAAATAGAAAATATAATATAACGAATCTATATAAATAAGCCACCAATATTTACACCTCGATCTGAAAAGGTCATCATTTCAGTTCGAGGttt < 4:301232/141‑1 (MQ=255) aaTCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGttt > 4:38615/1‑141 (MQ=255) tcTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTttttat > 2:719414/1‑141 (MQ=255) aGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTttttatttt < 1:128176/141‑1 (MQ=255) gaaGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTttttatttta > 1:528573/3‑141 (MQ=255) gCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTttttatttta > 3:171593/1‑141 (MQ=255) tttAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATTTTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTa < 4:343630/141‑1 (MQ=255) aaaCTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATggg > 2:656653/1‑141 (MQ=255) ctctTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTAt > 2:724032/1‑141 (MQ=255) ctTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTc > 2:42973/1‑141 (MQ=255) tttAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGATAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCt < 1:717584/141‑1 (MQ=255) tttAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCt > 3:126494/1‑141 (MQ=255) aaGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGGAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCtttt > 2:630983/1‑141 (MQ=255) aaGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCtttt > 3:307846/1‑141 (MQ=255) ggATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTAc < 2:602389/141‑1 (MQ=255) gATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACt < 4:59279/141‑1 (MQ=255) gATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACt > 1:568218/1‑141 (MQ=255) tATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGa < 4:293860/141‑1 (MQ=255) tATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGa < 4:74275/141‑1 (MQ=255) aTGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGaa > 3:32448/1‑141 (MQ=255) tGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGtaa > 2:91965/1‑141 (MQ=255) tGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGaaa < 2:351000/141‑1 (MQ=255) tGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGaaa < 1:258192/141‑1 (MQ=255) ttAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAAtt < 1:839600/141‑1 (MQ=255) aaTGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTg > 1:426253/1‑141 (MQ=255) tGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGtt < 2:232383/141‑1 (MQ=255) aaaaTAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGtttt < 1:452003/141‑1 (MQ=255) aaTAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTcc > 2:961438/1‑141 (MQ=255) aaTAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTcc < 2:575620/141‑1 (MQ=255) aTAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGGGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCt < 1:212820/141‑1 (MQ=255) aTAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCt > 4:288486/1‑141 (MQ=255) aTAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCt > 2:298552/1‑141 (MQ=255) aTAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCt < 2:194176/141‑1 (MQ=255) tAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTg < 1:823247/141‑1 (MQ=255) tAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTg > 4:259032/1‑141 (MQ=255) gaATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGtttt < 2:866924/141‑1 (MQ=255) taatataaCGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTAt < 3:47858/141‑1 (MQ=255) tataaCGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGt < 1:1014301/141‑1 (MQ=255) aaCGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTaaa < 1:1085311/141‑1 (MQ=255) aCGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAAt < 4:32448/141‑1 (MQ=255) aCGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAAt > 1:389315/1‑141 (MQ=255) gAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAAttt < 1:653480/141‑1 (MQ=255) gAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAAttt < 1:320803/141‑1 (MQ=255) tCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTtta > 2:170939/1‑141 (MQ=255) tatataAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTATATTTttatt > 2:867108/1‑141 (MQ=255) atataAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTttatta > 3:68923/1‑141 (MQ=255) atataAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTAAATGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTttatta > 3:7980/1‑141 (MQ=255) taaataaGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACAt > 2:389898/1‑141 (MQ=255) aaataaGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATa < 2:529560/141‑1 (MQ=255) taaGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTa < 4:129783/141‑1 (MQ=255) taaGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTa < 4:15401/141‑1 (MQ=255) taaGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTa < 1:153741/141‑1 (MQ=255) ggCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATct > 2:938645/1‑140 (MQ=255) cacaAATATTTAAACCTCGAGCTGAAAAGATCATTATTTCAGTTCGAAGTTTTTTATTTTATACTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTa < 4:79/141‑1 (MQ=255) ccAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGATTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTTCATAGTATCCTAAg > 2:937895/1‑141 (MQ=255) cAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGt < 1:406710/141‑1 (MQ=255) aaTATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTTTTTTTTTTTTTTATTACATATTATCCTAAttt > 4:398129/1‑141 (MQ=255) atTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTTTTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTtata > 2:38010/1‑141 (MQ=255) ttAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATc < 4:171593/141‑1 (MQ=255) tAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATcc < 1:554169/141‑1 (MQ=255) aCCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGa < 4:126494/141‑1 (MQ=255) aCCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGa < 1:724032/141‑1 (MQ=255) aCCTCGAACTGAAATGATGATCTTTTCAGCTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTTTTGTATTTTTTTATTACATCGTATCCTAAGTTATATCCTgt > 2:287175/1‑140 (MQ=37) cTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGACa < 1:709958/141‑1 (MQ=255) cTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGACa > 1:806905/1‑141 (MQ=255) tCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGACAt < 2:410864/141‑1 (MQ=255) tCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGACAt < 3:291894/141‑1 (MQ=255) cgaacTGAAATGATGATCTTTTCAGCTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAACTTTTTATTACATAGTATCCTAAGTTATATCCTGACAtt > 3:373848/5‑141 (MQ=38) cGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGACAtt < 4:175238/141‑1 (MQ=255) gCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGACATTTGt > 2:15613/1‑141 (MQ=255) gCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGACATTTGt > 1:480142/1‑141 (MQ=255) gCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATCTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGACATTTGt > 1:783122/1‑141 (MQ=255) cTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATGCTAAGTTATATCCTGACATTTGtt > 4:245083/1‑141 (MQ=255) cTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGACATTTGtt > 2:456928/1‑141 (MQ=255) cTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGACATTTGtt > 2:642502/1‑141 (MQ=255) gAAATGATGATCTTTTCAGCTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTACTG‑TTTTTTATTGTACATTTTTATTACATAGTATCCTAAGTTATATCCTGACATTTGTtctc > 1:972687/1‑141 (MQ=255) aaaGATCATCATTTCAGTTCGTGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGACATTTGTTCTcc < 1:652884/141‑1 (MQ=255) aaaGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGACATTTGTTATcc > 1:1071164/1‑141 (MQ=255) aaGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGACATTTGTTCTCCa < 4:413095/141‑1 (MQ=255) aaGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGACATTTGTTCTCCa < 1:1080744/141‑1 (MQ=255) catcatTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGACATTTGTTCTCCAGTAtt < 1:900850/141‑1 (MQ=255) | TGATGAAACATTTTTGAATCATCTAGCAGGTATTAATTTAAAACTCTTTAAGGATTATGTTAATGAAAATAGAGAATATAATATAACGAATCTATATAAATAAGGCACCAATATTTAAACCTCGAGCTGAAAAGATCATCATTTCAGTTCGAGGTTTTTTATTTTATCCTCTTTGTATGGGTATTCTTTTACTTGAAATTTGTTTTCCTGTTTTTTTATTGTAAATTTTTATTACATAGTATCCTAAGTTATATCCTGACATTTGTTCTCCAGTATT > USA300TCH1516_ALE/168218‑168494 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |