BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,528,064	C→T	5.6%	V46V (GTG→GTA)	USA300TCH1516_02412 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,528,064	0	C	T	5.6%	448.2 / 10.4	143	V46V (GTG→GTA)	USA300TCH1516_02412	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (70/65); new base T (6/2); total (76/67)
Fisher's exact test for biased strand distribution p-value = 2.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-01

Only 100 of 147 total aligned reads displayed.

TGAGGTGTCGCAAATGAGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCT  >  USA300TCH1516_ALE/2527924‑2528203

 gAGGTGTCGCAAATGAGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAaca                                                                                                                                            <  1:478836/141‑1 (MQ=255)

  aGGTGTCGCAAATGAGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAacac                                                                                                                                           >  4:147926/1‑141 (MQ=255)

    gtgtCGCAAATGAGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACtt                                                                                                                                         >  1:238007/1‑141 (MQ=255)

           aaaTGAGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACt                                                                                                                                  <  1:54533/141‑1 (MQ=255)

            aaTGGGTCAATATGTTCAGTTAAATATGGACGCTTAGCCGTTTTATATTCTGCTAATGTATTGGTGGTCATTGTAGGTGTTTTGATCGCATAAGAGGCATCACCGCCTGAATCTGCATTTAGGCGAAACGCTTTGGTACTa                                                                                                                                 <  4:86822/141‑1 (MQ=255)

            aaTGAGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTa                                                                                                                                 <  3:345280/141‑1 (MQ=255)

             aTGAGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATGGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAg                                                                                                                                >  4:222132/1‑141 (MQ=255)

              tGAGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGc                                                                                                                               <  2:613584/141‑1 (MQ=255)

                  tCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCt                                                                                                                           >  1:547513/1‑141 (MQ=255)

                   cAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCtt                                                                                                                          >  1:207450/1‑141 (MQ=255)

                    aaTATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCttt                                                                                                                         >  2:1086189/1‑141 (MQ=255)

                    aaTATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCACATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCttt                                                                                                                         <  1:455462/141‑1 (MQ=255)

                       atatTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTgg                                                                                                                      <  1:308261/141‑1 (MQ=255)

                         atTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGAt                                                                                                                    >  1:131892/1‑141 (MQ=255)

                          ttCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATg                                                                                                                   >  4:35722/1‑141 (MQ=255)

                          ttCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATg                                                                                                                   <  3:384524/141‑1 (MQ=255)

                            cAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGaa                                                                                                                 >  1:147424/1‑141 (MQ=255)

                             aaTTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGaaa                                                                                                                >  1:240807/1‑141 (MQ=255)

                              aTTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAAc                                                                                                               >  2:80078/1‑141 (MQ=255)

                              aTTAAATATGGACGCTTAACCGTTTTAGATTCTGCTGATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATACAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACAATTTGATTCTAGCGTCTTTTGGATGAAAc                                                                                                               >  2:675068/1‑141 (MQ=255)

                                  aaTATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACAAtt                                                                                                           >  3:9055/1‑141 (MQ=255)

                                   atatGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCAttt                                                                                                          >  1:994010/1‑141 (MQ=255)

                                     atGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCAttttt                                                                                                        <  3:290683/141‑1 (MQ=255)

                                      tGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTc                                                                                                       >  1:892974/1‑141 (MQ=255)

                                        gACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCaa                                                                                                     <  1:280796/141‑1 (MQ=255)

                                        gACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCaa                                                                                                     >  4:233041/1‑141 (MQ=255)

                                             ttAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAaataat                                                                                                <  1:688836/141‑1 (MQ=255)

                                               aaCCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGc                                                                                              <  1:421141/141‑1 (MQ=255)

                                                   tttttATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCtttt                                                                                          <  2:610239/140‑1 (MQ=255)

                                                   gTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTNGATGAAACCATTNTTCAAATAATGCtttt                                                                                          <  1:730903/141‑1 (MQ=255)

                                                   gTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCtttt                                                                                          <  1:786207/141‑1 (MQ=255)

                                                      ttATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTc                                                                                       >  4:135381/1‑141 (MQ=255)

                                                      ttATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTc                                                                                       >  3:376598/1‑141 (MQ=255)

                                                      ttATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTc                                                                                       >  3:413061/1‑141 (MQ=255)

                                                       tataTTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCg                                                                                      <  3:191351/141‑1 (MQ=255)

                                                        atatTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGt                                                                                     <  4:17865/141‑1 (MQ=255)

                                                           ttCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACCCTTTGGTACTAGCGTCTTTAGGATGAAACCATTTATCAAATAATGCTTTTGTCGtata                                                                                  <  3:178073/141‑1 (MQ=255)

                                                           ttCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGtata                                                                                  >  2:507368/1‑141 (MQ=255)

                                                                cTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCAt                                                                             <  4:412415/141‑1 (MQ=255)

                                                                  aaTGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCa                                                                           <  1:794541/141‑1 (MQ=255)

                                                                  aaTGTATAGCTGGTCATTGTAGGTGTTGTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCa                                                                           <  2:150976/141‑1 (MQ=255)

                                                                   aTGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTCGCGTCTTTTGGATGAACCCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCaa                                                                          <  4:146341/141‑1 (MQ=255)

                                                                   aTGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCaa                                                                          >  1:366712/1‑141 (MQ=255)

                                                                       ataGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAacac                                                                      <  1:482107/141‑1 (MQ=255)

                                                                        taGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACAcc                                                                     <  1:1023512/141‑1 (MQ=255)

                                                                           cTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCttt                                                                  >  1:660753/1‑141 (MQ=255)

                                                                            tGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCtttt                                                                 <  3:182998/141‑1 (MQ=255)

                                                                                 aTTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATc                                                            <  3:27675/141‑1 (MQ=255)

                                                                                  ttGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCg                                                           >  2:724492/1‑141 (MQ=255)

                                                                                    gTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGt                                                         >  3:130352/1‑141 (MQ=255)

                                                                                     tAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATAAATCACCTTTTCAATCGGTg                                                        >  1:826644/1‑141 (MQ=255)

                                                                                       ggTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGCAACCATTTTTCCAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCt                                                      <  2:226852/141‑1 (MQ=255)

                                                                                       ggTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCt                                                      <  2:488853/141‑1 (MQ=255)

                                                                                           ttttAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCTTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTa                                                  >  2:462877/1‑141 (MQ=255)

                                                                                           ttttAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTa                                                  <  3:278886/141‑1 (MQ=255)

                                                                                              tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                               >  1:271390/1‑141 (MQ=255)

                                                                                              tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                               <  2:190226/141‑1 (MQ=255)

                                                                                               aaTCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGt                                              >  2:46564/1‑141 (MQ=255)

                                                                                                 tCGCATAAAATGCATCACCGCCTGGAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTc                                            <  1:897861/141‑1 (MQ=255)

                                                                                                 tCGCATAAAATGCATCACCGCCTGGAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTc                                            <  1:897939/141‑1 (MQ=255)

                                                                                                 acGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTc                                            <  1:908253/140‑1 (MQ=255)

                                                                                                   gCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCTGTGCTTCTCtctttctt                                          >  4:285549/1‑141 (MQ=255)

                                                                                                    cATAAAATGCATCACCGCCTGAAACAGCATTAACGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCATTCTTg                                         >  2:582142/1‑141 (MQ=255)

                                                                                                     aTAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGa                                        >  4:182347/1‑141 (MQ=255)

                                                                                                      tAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGaa                                       >  4:244566/1‑141 (MQ=255)

                                                                                                       aaaaTGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGaaa                                      <  3:171301/141‑1 (MQ=255)

                                                                                                          aTGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGaaaaaa                                   >  2:929869/1‑141 (MQ=255)

                                                                                                          aTGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGaaaaaa                                   >  1:358462/1‑141 (MQ=255)

                                                                                                            gCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAaata                                 >  4:264661/1‑141 (MQ=255)

                                                                                                            gCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAaata                                 >  1:393203/1‑141 (MQ=255)

                                                                                                               tCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAtt                              >  1:1020457/1‑141 (MQ=255)

                                                                                                               tCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAtt                              <  3:135799/141‑1 (MQ=255)

                                                                                                                cACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAttt                             >  4:419341/1‑141 (MQ=255)

                                                                                                                cACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAttt                             <  3:296216/141‑1 (MQ=255)

                                                                                                                 aCCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTATCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAtttt                            <  1:210974/141‑1 (MQ=255)

                                                                                                                 aCCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAtttt                            <  3:88914/141‑1 (MQ=255)

                                                                                                                 aCCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTATTCGAAAAATAAtttt                            >  1:332266/1‑141 (MQ=255)

                                                                                                                        gAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATATTGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCt                     >  2:651658/1‑141 (MQ=255)

                                                                                                                        gAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCgg                                                          <  4:191142/104‑1 (MQ=255)

                                                                                                                        gAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCt                     >  3:37384/1‑141 (MQ=255)

                                                                                                                          aaCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTc                   >  2:429856/1‑141 (MQ=255)

                                                                                                                          aaCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTc                   >  1:336439/1‑141 (MQ=255)

                                                                                                                          aaCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTc                   >  4:79316/1‑141 (MQ=255)

                                                                                                                          aaCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTc                   <  2:726621/141‑1 (MQ=255)

                                                                                                                           aCAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCa                  <  2:1045680/141‑1 (MQ=255)

                                                                                                                           aCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCa                  >  2:623621/1‑141 (MQ=255)

                                                                                                                             aGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAAc                >  3:336365/1‑141 (MQ=255)

                                                                                                                                aTTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTTTTTTTTTTTTTTTCACATATAGGCATCAAACACCTTTTCAATCGGTTTTTCTATCGATCTTGTAATCATACTTTTATCATCTACAACtat             >  2:1045045/1‑139 (MQ=255)

                                                                                                                                aTTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTTTTTTTTTTTCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACttt             >  2:350338/1‑141 (MQ=255)

                                                                                                                                   aaaGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAAt          >  1:364319/1‑141 (MQ=255)

                                                                                                                                   aaaGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAAt          <  1:1061227/141‑1 (MQ=255)

                                                                                                                                    aaacGACACACTTTTGTACTACCCTCTTTTTGATTAAACCATTATTCAATTAATGCTTTTGTCTTATTTTCCTCAAACACCTTTTCAATCGCTACTTCTACAGATCAAAAAAAAATAATTTTATTATCTTCAACTTTAATc         <  2:1015214/138‑1 (MQ=255)

                                                                                                                                    aaGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATc         <  3:285443/141‑1 (MQ=255)

                                                                                                                                    aaGCGAAACACTTTGGTACTAGAGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTAAAGATCATGAAAAAATAATTTTATTATCTTCAACTTTAATc         <  4:18536/141‑1 (MQ=255)

                                                                                                                                     aGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCg        >  1:463584/1‑141 (MQ=255)

                                                                                                                                      gCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGt       >  2:196261/1‑141 (MQ=255)

                                                                                                                                       cGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTc      <  1:963827/141‑1 (MQ=255)

                                                                                                                                        gAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCa     >  1:253099/1‑141 (MQ=255)

                                                                                                                                          aaCACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATc   <  2:626878/141‑1 (MQ=255)

                                                                                                                                          aaCACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTATAAAAAAAAAAAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATc   <  2:239270/141‑1 (MQ=255)

TGAGGTGTCGCAAATGAGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCT  >  USA300TCH1516_ALE/2527924‑2528203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`