Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,394,482 T→A 6.2% D27V (GAT→GTT)  moaB ← Molybdenum cofactor biosynthesis protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,394,4820TA 6.2% 349.1 / 10.5 113D27V (GAT→GTT) moaBMolybdenum cofactor biosynthesis protein B
Reads supporting (aligned to +/- strand):  ref base T (43/62);  new base A (3/4);  total (46/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

Only 100 of 113 total aligned reads displayed.

AATATCTTCTTCTAACCACTTCTTCACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCTTTTTTTATCCAAACAATAAATTCATTAGATG  >  USA300TCH1516_ALE/2394343‑2394618
                                                                                                                                           |                                                                                                                                        
aaTATCTTCTTCTAACCACTTCTTCACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATc                                                                                                                                         <  3:416347/141‑1 (MQ=255)
   atCTTCTTCTAACCACTTCTTCACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCaaa                                                                                                                                      >  2:1044734/1‑141 (MQ=255)
    tcttcttctAACCACTTCTTCACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAg                                                                                                                                     <  1:808695/141‑1 (MQ=255)
     cttcttctAACCACTTCTTCACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCATCGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTAACAAAGt                                                                                                                                    <  2:190245/141‑1 (MQ=255)
      ttcttctAACCACTTCTTCACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCACACGCATTGACCACCTTTATCTGTATCAAAGtc                                                                                                                                   >  4:344963/1‑141 (MQ=255)
        cttctAACCACTTCTTCACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGtctc                                                                                                                                 >  2:1066157/1‑141 (MQ=255)
         ttctAACCACTTCTTCACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTctct                                                                                                                                >  2:321194/1‑141 (MQ=255)
         ttctAACCACTTCTTCACCGGCGTCGTGATGGCTACTTTTTCATCTTTCACAAGTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTctct                                                                                                                                <  2:466821/141‑1 (MQ=255)
           ctAACCACTTCTTCACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAg                                                                                                                              <  1:145016/141‑1 (MQ=255)
             aaCCACTTCTTCACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTa                                                                                                                            >  3:295560/1‑141 (MQ=255)
             aaCCACTTCTTCACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTa                                                                                                                            >  3:193404/1‑141 (MQ=255)
               ccACTTCTTCACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATc                                                                                                                          <  4:406999/141‑1 (MQ=255)
                  cttcttCACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGa                                                                                                                       >  2:207101/1‑141 (MQ=255)
                   ttcttcACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGAt                                                                                                                      >  4:273519/1‑141 (MQ=255)
                     cttcACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATAc                                                                                                                    <  3:94052/141‑1 (MQ=255)
                     cttcACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATAc                                                                                                                    >  3:17055/1‑141 (MQ=255)
                     cttcACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTAACAAAGTCTCTAGTATCTGATAc                                                                                                                    >  2:83706/1‑141 (MQ=255)
                     cttcACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATAGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATAc                                                                                                                    <  2:934094/141‑1 (MQ=255)
                       tcACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCg                                                                                                                  >  1:908846/1‑141 (MQ=255)
                       tcACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCg                                                                                                                  >  1:976775/1‑141 (MQ=255)
                          ccTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTa                                                                                                               >  1:159780/1‑141 (MQ=255)
                          ccTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTa                                                                                                               >  1:618717/1‑141 (MQ=255)
                            tGCGTCGTGATGGCTACTTTTTCATCTTTCACACTTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAATGTCTCTAGTATCTCATACCCTTAGt                                                                                                             >  2:670676/1‑141 (MQ=255)
                                tcgtGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACgg                                                                                                         <  1:805212/141‑1 (MQ=255)
                                tcgtGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACgg                                                                                                         <  1:146087/141‑1 (MQ=255)
                                 cgtGATGTCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGgc                                                                                                        <  4:318495/141‑1 (MQ=255)
                                 cgtGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGgc                                                                                                        >  2:544159/1‑141 (MQ=255)
                                 cgtGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGgc                                                                                                        >  1:877313/1‑141 (MQ=255)
                                    gATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGgctgc                                                                                                     >  2:222373/1‑141 (MQ=255)
                                     aTGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGgctgct                                                                                                    <  4:433201/141‑1 (MQ=255)
                                           aCTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTAACAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACt                                                                                              <  1:411198/141‑1 (MQ=255)
                                            cTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATACTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTg                                                                                             >  1:623474/1‑141 (MQ=255)
                                                  cATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGAt                                                                                       <  2:225572/141‑1 (MQ=255)
                                                   atatttCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGAtt                                                                                      >  2:382213/4‑141 (MQ=255)
                                                    tCTTTCACAATTGTATAATGTGCGTCCCGCACTTCAACGTCATCCGCTTGTAATAGTTGTCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGCTAGTACGGCTGCTTTAACTGTACGGTTc                                                                                     >  2:392467/1‑141 (MQ=255)
                                                     cTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCa                                                                                    <  1:391917/141‑1 (MQ=255)
                                                        tCACAATTGTATAATGTGCGTGACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTGGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAAtt                                                                                 <  2:1027174/141‑1 (MQ=255)
                                                        tCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAAtt                                                                                 <  4:343406/141‑1 (MQ=255)
                                                          acaATTGTATAAGGTGCTGCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTTACCACCTTTATCTGTATCAAAGTCTCTATTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTa                                                                               <  4:177889/141‑1 (MQ=255)
                                                             aTTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACg                                                                            <  4:197108/141‑1 (MQ=255)
                                                               tGTATAATGTGCGTCANTCACTTCAACGTNATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGtt                                                                          >  1:626224/1‑141 (MQ=255)
                                                               tGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGtt                                                                          >  3:58212/1‑141 (MQ=255)
                                                               tGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGtt                                                                          >  3:406573/1‑141 (MQ=255)
                                                               tGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGtt                                                                          <  2:31368/141‑1 (MQ=255)
                                                                gTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGttt                                                                         <  2:988798/141‑1 (MQ=255)
                                                                gTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCCTTAACTGTACGATTCAATTTAACGttt                                                                         >  1:70399/1‑141 (MQ=255)
                                                                   taATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGa                                                                      >  1:81284/1‑141 (MQ=255)
                                                                   taATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTAACAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGa                                                                      >  2:348571/1‑141 (MQ=255)
                                                                     aTGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTCTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATg                                                                    >  2:955426/1‑141 (MQ=255)
                                                                       gtgCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGtt                                                                  <  1:1057322/141‑1 (MQ=255)
                                                                        tgCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTc                                                                 <  2:1018650/141‑1 (MQ=255)
                                                                          cGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGc                                                               >  1:249849/1‑141 (MQ=255)
                                                                            tcactcacTTCATCGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGccc                                                             <  2:532289/141‑1 (MQ=255)
                                                                            tcactcacTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGccc                                                             <  1:778182/141‑1 (MQ=255)
                                                                              actcactTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCat                                                           <  3:140876/141‑1 (MQ=255)
                                                                                    ttCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCGTCTGTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATAtt                                                     <  3:230184/141‑1 (MQ=255)
                                                                                    ttCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTCTCAAAGTCTCTAGTATCTGCCACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTACCGTTTTGATGTTCGCCCATAATAtt                                                     <  3:386570/141‑1 (MQ=255)
                                                                                      cAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTac                                                   <  4:223568/141‑1 (MQ=255)
                                                                                       aaCGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTaca                                                  <  1:59794/141‑1 (MQ=255)
                                                                                        ccGTCATCTGCTTGTAATAGTTGGCTCACGCATTGTCCACCTTTATCTGTATCAAAGTCTCTAGTATCTTATACCGTTAGTACGGCTGCTTTACCTGTACGATCCCATCTATGGTTTTGATGTTCGCCCATAATATTacac                                                 <  4:363549/140‑1 (MQ=255)
                                                                                        aCGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTacac                                                 <  1:656191/141‑1 (MQ=255)
                                                                                         cGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACt                                                <  3:19405/141‑1 (MQ=255)
                                                                                         cGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACt                                                >  1:537890/1‑141 (MQ=255)
                                                                                         cGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTAACAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACt                                                <  3:375684/141‑1 (MQ=255)
                                                                                           tCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTcc                                              <  1:116453/141‑1 (MQ=255)
                                                                                           tCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTcc                                              >  1:397837/1‑141 (MQ=255)
                                                                                           tCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATCTAACGTTTTGATGTTCGCCCATAATATTACACTcc                                              <  4:199927/141‑1 (MQ=255)
                                                                                             aTCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCtt                                            <  2:351245/141‑1 (MQ=255)
                                                                                                tGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTg                                         <  2:101565/141‑1 (MQ=255)
                                                                                                tGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTg                                         <  2:749623/141‑1 (MQ=255)
                                                                                                tGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTg                                         <  4:304616/141‑1 (MQ=255)
                                                                                                tGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTg                                         >  1:321165/1‑141 (MQ=255)
                                                                                                 gCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGt                                        >  1:156071/1‑141 (MQ=255)
                                                                                                   ttGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCt                                      <  1:222373/141‑1 (MQ=255)
                                                                                                      tAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTAt                                   <  3:26522/141‑1 (MQ=255)
                                                                                                        aTAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCt                                 <  2:860717/141‑1 (MQ=255)
                                                                                                          aGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTAGGATTCAATTTACAGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCttt                               >  1:702258/1‑141 (MQ=255)
                                                                                                           gTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCtttt                              <  3:159791/141‑1 (MQ=255)
                                                                                                           gTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCtttt                              <  1:1032888/141‑1 (MQ=255)
                                                                                                            ttGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCttttt                             <  1:187985/141‑1 (MQ=255)
                                                                                                                cgcacgcaTTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCttttttttt                         >  2:744790/1‑139 (MQ=255)
                                                                                                                   acgcaTTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCTTTTTTTATCCa                      >  1:596593/1‑141 (MQ=255)
                                                                                                                      caTTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCTTTTTTTATCCAAAc                   <  2:404773/141‑1 (MQ=255)
                                                                                                                        ttGACCACCTTTATCTGTATCCAAGTCTTTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCTTTTTTTATCCAAACaa                 >  1:27563/1‑141 (MQ=255)
                                                                                                                        ttGACCACCTTTATCTGTATCAAAGTCTCTGGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTAGCCCATAATATTACACTCCTTTTGTCTTATCTTTTTTTATCCAAACaa                 >  2:1013687/1‑141 (MQ=255)
                                                                                                                         tGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCTTTTTTTATCCAAACAAt                <  1:321194/141‑1 (MQ=255)
                                                                                                                         tGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCTTTTTTTATCCAAACAAt                <  2:449998/141‑1 (MQ=255)
                                                                                                                         tGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCTTTTTTTATCCAAACAAt                <  2:83626/141‑1 (MQ=255)
                                                                                                                          gACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCTTTTTTTATCCAAACAATa               <  1:930365/141‑1 (MQ=255)
                                                                                                                          gACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCTTTTTTTATCCAAACAATa               >  1:60608/1‑141 (MQ=255)
                                                                                                                          gACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCTTTTTTTATCCAAACAATa               <  4:114050/141‑1 (MQ=255)
                                                                                                                          gACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCTTTTTTTATCCAAACAATa               <  3:54040/141‑1 (MQ=255)
                                                                                                                            ccaccTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCTTTTTTTATCCAAACAATaaa             >  2:227325/1‑141 (MQ=255)
                                                                                                                            ccaccTTTATCTGTAACAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCTTTTTTTATCCAAACAATaaa             <  1:83706/141‑1 (MQ=255)
                                                                                                                                cTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTCACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTATTGTCTTATCTTTTTTTATCCAAACAATAAAttta         >  4:348247/1‑139 (MQ=255)
                                                                                                                                 tttATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCTTTTTTTATCCAAACAATAAATTCAt        <  3:69687/141‑1 (MQ=255)
                                                                                                                                   tATCTGTCTCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTTCGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACATTCCTTTAGTCTTATCTTTTTGTATCCGAACAATAAATTCATTa      <  4:425072/141‑1 (MQ=255)
                                                                                                                                    aTCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCTTTTTTTATCCAAACAATAAATTCATTAg     <  2:803962/141‑1 (MQ=255)
                                                                                                                                      cTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCTTTTTTTATCCAAACAATAAATTCATTAGAt   <  2:615486/141‑1 (MQ=255)
                                                                                                                                       tGTATCAAAGTCTCTAGTATCTGATCCCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTCTCTCTTTTTATCCTATTATTAAATTCATTAGAtg  <  2:545905/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
AATATCTTCTTCTAACCACTTCTTCACCTGCGTCGTGATGGCTACTTTTTCATCTTTCACAATTGTATAATGTGCGTCACTCACTTCAACGTCATCTGCTTGTAATAGTTGGCGCACGCATTGACCACCTTTATCTGTATCAAAGTCTCTAGTATCTGATACCGTTAGTACGGCTGCTTTAACTGTACGATTCAATTTAACGTTTTGATGTTCGCCCATAATATTACACTCCTTTTGTCTTATCTTTTTTTATCCAAACAATAAATTCATTAGATG  >  USA300TCH1516_ALE/2394343‑2394618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑