Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,391,457 C→T 5.4% A95A (GCG→GCA moaE ← Molybdopterin synthase catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,391,4570CT 5.4% 404.7 / 8.5 129A95A (GCG→GCAmoaEMolybdopterin synthase catalytic subunit
Reads supporting (aligned to +/- strand):  ref base C (69/52);  new base T (4/3);  total (74/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

Only 100 of 131 total aligned reads displayed.

TCATAATTCCCTTTTTGATGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAATATACGCTTCATA  >  USA300TCH1516_ALE/2391317‑2391597
                                                                                                                                            |                                                                                                                                            
tcATAATTCCCTTTTTGATGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAAcc                                                                                                                                              >  1:505833/1‑141 (MQ=255)
 cATAATTCCCTTTTTGATGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCg                                                                                                                                             <  2:72016/141‑1 (MQ=255)
   tAATTCCCTTTTTGATGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACTGCa                                                                                                                                           >  1:803811/1‑141 (MQ=255)
    aaTTCCCTTTTTGATGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCaa                                                                                                                                          <  1:3922/141‑1 (MQ=255)
     aTTCCCTTTTTGATGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAAt                                                                                                                                         <  1:1075853/141‑1 (MQ=255)
     aTTCCCTTTTTGATGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATGGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAAt                                                                                                                                         >  2:98682/1‑141 (MQ=255)
        cccTTTTTGATGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTaa                                                                                                                                      <  1:1048056/141‑1 (MQ=255)
        cccTTTTTGATGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTaa                                                                                                                                      <  3:436311/141‑1 (MQ=255)
        cccTTTTTGATGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTaa                                                                                                                                      >  1:298890/1‑141 (MQ=255)
         ccTTTTTGATGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAAt                                                                                                                                     >  1:583136/1‑141 (MQ=255)
         ccTTTTTGATGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAAt                                                                                                                                     >  1:403704/1‑141 (MQ=255)
          cTTTTTGATGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGTTGAAGAAACCGCAATTAATa                                                                                                                                    >  2:642704/1‑141 (MQ=255)
          cTTTTTGATGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATa                                                                                                                                    >  1:842756/1‑141 (MQ=255)
          cTTTTTGATGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATa                                                                                                                                    >  1:454147/1‑141 (MQ=255)
           tttttGATGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACTGCAATTAATAc                                                                                                                                   >  2:60147/1‑141 (MQ=255)
                gATGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGa                                                                                                                              >  1:854631/1‑141 (MQ=255)
                  tGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGata                                                                                                                            >  3:110123/1‑141 (MQ=255)
                   gCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCCATTAATACAGCGatat                                                                                                                           >  3:150671/1‑141 (MQ=255)
                   gCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGatat                                                                                                                           <  1:1060841/141‑1 (MQ=255)
                   gCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGatat                                                                                                                           >  1:743337/1‑141 (MQ=255)
                   gCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGatat                                                                                                                           >  4:272466/1‑141 (MQ=255)
                    tttttttcATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATTTTCATTTTCTCTATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATc                                                                                                                          >  2:342018/8‑141 (MQ=255)
                    cccTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATc                                                                                                                          <  3:435756/141‑1 (MQ=255)
                            aTTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAAttt                                                                                                                  <  4:251073/141‑1 (MQ=255)
                               ttGAACCATCTTCCCAAATTTCTTTTTACAAAATCGGAACAATTTCTTTTATACGCTCAATTGTATATTCATTTGCTCGATAGGCCTCTTTACGTTGCGGTGAAGAAACCGCAATTAATACAGCGATATGTGGAATTTGTa                                                                                                               >  2:981673/1‑141 (MQ=255)
                                tGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTaa                                                                                                              >  1:894833/1‑141 (MQ=255)
                                 gAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAAt                                                                                                             >  2:121955/1‑141 (MQ=255)
                                  aaCCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATg                                                                                                            <  1:745341/141‑1 (MQ=255)
                                    ccATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGc                                                                                                          <  2:97508/141‑1 (MQ=255)
                                    ccATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGc                                                                                                          <  2:642713/141‑1 (MQ=255)
                                    ccATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGc                                                                                                          >  2:799914/1‑141 (MQ=255)
                                    ccATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGc                                                                                                          <  1:175861/141‑1 (MQ=255)
                                     cATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGcc                                                                                                         >  2:900929/1‑141 (MQ=255)
                                     cATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGcc                                                                                                         >  2:395997/1‑141 (MQ=255)
                                      aTCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGccc                                                                                                        >  1:237003/1‑141 (MQ=255)
                                      aTCTACCCAAATTTCTTTTTTCCAAATCGGAACAACTTCTTTTATATGCTAAATTGCATATTCACTTCCTCGATACGCATCTTTACGATGCGGTGAAGAAACAGCAATTCATACAGCGATATCTGAATTTTGTTATgtcgc                                                                                                        >  2:1033289/1‑137 (MQ=255)
                                        cTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTa                                                                                                      <  2:681519/141‑1 (MQ=255)
                                             cAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTa                                                                                                 >  3:18423/1‑141 (MQ=255)
                                             cAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTa                                                                                                 >  1:920831/1‑141 (MQ=255)
                                              aaaTTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTAt                                                                                                >  1:751863/1‑141 (MQ=255)
                                               aaTTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATg                                                                                               >  3:192605/1‑141 (MQ=255)
                                               aaTTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATg                                                                                               <  2:225154/141‑1 (MQ=255)
                                                aTTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGa                                                                                              <  3:156041/141‑1 (MQ=255)
                                                     ttttttCCAAATCGGAACAATTTCTTTTATACGCTCCATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAAt                                                                                         <  1:650477/141‑1 (MQ=255)
                                                     ttttttCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAAt                                                                                         >  2:335322/1‑141 (MQ=255)
                                                     ttttttCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAAt                                                                                         >  1:304445/1‑141 (MQ=255)
                                                       ttttCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATAc                                                                                       <  1:686980/141‑1 (MQ=255)
                                                       ttttCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATAc                                                                                       >  1:931669/1‑141 (MQ=255)
                                                        tttCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACt                                                                                      <  2:836959/141‑1 (MQ=255)
                                                          tCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCg                                                                                    >  3:345739/1‑141 (MQ=255)
                                                           ccAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGt                                                                                   >  2:517730/1‑141 (MQ=255)
                                                              aaTCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTGTGAACAATACTCGTTAt                                                                                >  1:8793/1‑141 (MQ=255)
                                                              aaTCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTAt                                                                                >  1:887505/1‑141 (MQ=255)
                                                              aaTCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTAt                                                                                >  1:587140/1‑141 (MQ=255)
                                                               aTCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGCACAATACTCGTTATc                                                                               <  2:496144/141‑1 (MQ=255)
                                                               aTCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATc                                                                               >  3:71583/1‑141 (MQ=255)
                                                                    aaCAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATATGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAACTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTcc                                                                          >  4:242323/1‑141 (MQ=255)
                                                                    aaCAATTTCTTGTCTTCGCTCCATTGCATATTCATTTGCTGGAGAGGCATCTTTACGATGCGGTGGAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTATTCGCCCTATTCTATGAACAATACTCGTTATCGTTcc                                                                          <  4:132978/141‑1 (MQ=255)
                                                                      cAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAg                                                                        >  1:353933/1‑141 (MQ=255)
                                                                        aTTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACTGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGc                                                                      <  4:170766/141‑1 (MQ=255)
                                                                        aTTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGc                                                                      <  1:501209/141‑1 (MQ=255)
                                                                         tttCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGcc                                                                     <  2:901350/141‑1 (MQ=255)
                                                                          ttCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCa                                                                    >  1:108258/1‑141 (MQ=255)
                                                                            cTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCAtt                                                                  >  2:128434/1‑141 (MQ=255)
                                                                                tataCGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTc                                                              >  3:364840/1‑141 (MQ=255)
                                                                                   aCGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCatt                                                           >  1:80014/1‑141 (MQ=255)
                                                                                   aCGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCatt                                                           <  2:893407/141‑1 (MQ=255)
                                                                                     gCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCattta                                                         >  1:608857/1‑141 (MQ=255)
                                                                                           ttGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCAt                                                   >  1:818681/1‑141 (MQ=255)
                                                                                              cATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCAtctc                                                >  2:514328/1‑141 (MQ=255)
                                                                                              cATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCAtctc                                                >  1:39214/1‑141 (MQ=255)
                                                                                              cATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCAtctc                                                >  1:346687/1‑141 (MQ=255)
                                                                                              cATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAAGTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCAtctc                                                >  4:124698/1‑141 (MQ=255)
                                                                                               atatTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTcc                                               >  1:747835/1‑141 (MQ=255)
                                                                                                   tCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAAtt                                           <  3:313197/141‑1 (MQ=255)
                                                                                                      tttGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATATCCAATTtgt                                        >  2:373740/1‑141 (MQ=255)
                                                                                                          cTCGATAGGCATCTTTACGATGCGGTGAAGAAACTGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCa                                    >  1:38951/1‑141 (MQ=255)
                                                                                                            cGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAAt                                  <  4:147563/141‑1 (MQ=255)
                                                                                                               tAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTc                               >  4:35810/1‑141 (MQ=255)
                                                                                                               tAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTc                               >  2:276603/1‑141 (MQ=255)
                                                                                                                   cATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCtttt                           <  3:185543/141‑1 (MQ=255)
                                                                                                                   cATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCcttt                           <  2:140846/141‑1 (MQ=255)
                                                                                                                    aTCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTc                          >  1:636553/1‑141 (MQ=255)
                                                                                                                      cTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAg                        <  1:342018/141‑1 (MQ=255)
                                                                                                                      cTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAg                        >  1:174239/1‑141 (MQ=255)
                                                                                                                       tttACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGc                       <  2:505833/141‑1 (MQ=255)
                                                                                                                           cGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCAtt                   >  2:747930/1‑141 (MQ=255)
                                                                                                                             aTGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCATTTTGTGCCAATTTCTTTTCAGCCATTgg                 <  1:869265/141‑1 (MQ=255)
                                                                                                                             aTGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTgg                 <  1:268491/141‑1 (MQ=255)
                                                                                                                             aTGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTACGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTgg                 >  2:384167/1‑141 (MQ=255)
                                                                                                                              tGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGa                <  3:411277/141‑1 (MQ=255)
                                                                                                                               gCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGaa               <  3:223756/141‑1 (MQ=255)
                                                                                                                                cGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAat              <  2:237003/141‑1 (MQ=255)
                                                                                                                                 ggTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGCata             >  4:328972/1‑141 (MQ=255)
                                                                                                                                  gTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAatat            >  1:795080/1‑141 (MQ=255)
                                                                                                                                  gTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAatat            <  3:16050/141‑1 (MQ=255)
                                                                                                                                       gaaACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAATATACGCt       <  4:199335/141‑1 (MQ=255)
                                                                                                                                         aaCCGCAATTAATACAGCGATATCTGGAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAATATACGCTTc     <  2:861079/141‑1 (MQ=255)
                                                                                                                                          aCCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCACTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCACCTATTTGTTCCAATTTCTTTTCAGGCATTGGAATATACGCTTCa    <  4:314926/141‑1 (MQ=255)
                                                                                                                                            cGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAATATACGCTTCata  >  2:202862/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TCATAATTCCCTTTTTGATGCCCTTGCCATTTTGAACCATCTTCCCAAATTTCTTTTTTCCAAATCGGAACAATTTCTTTTATACGCTCAATTGCATATTCATTTGCTCGATAGGCATCTTTACGATGCGGTGAAGAAACCGCAATTAATACAGCGATATCTGAAATTTGTAATGGCCCTATTCTATGAACAATACTCGTTATCGTTCCAGGCCATTTTTCATTTATTTCATCTCCAATTTGTGCCAATTTCTTTTCAGCCATTGGAATATACGCTTCATA  >  USA300TCH1516_ALE/2391317‑2391597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: