Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,372,419 C→T 7.3% intergenic (+170/+22) USA300TCH1516_02262 → / ← pbuG hypothetical protein/Guanine/hypoxanthine permease PbuG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,372,4190CT 7.3% 469.2 / 15.1 154intergenic (+170/+22)USA300TCH1516_02262/pbuGhypothetical protein/Guanine/hypoxanthine permease PbuG
Reads supporting (aligned to +/- strand):  ref base C (75/67);  new base T (8/4);  total (83/71)
Fisher's exact test for biased strand distribution p-value = 3.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

ATATAGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCC  >  USA300TCH1516_ALE/2372279‑2372557
                                                                                                                                            |                                                                                                                                          
atataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGc                                                                                                                                            <  1:248716/141‑1 (MQ=255)
atataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCCCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGc                                                                                                                                            <  3:349753/141‑1 (MQ=255)
 tataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCa                                                                                                                                           <  1:911269/141‑1 (MQ=255)
  ataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATTGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAACGACTGCCAATGATTGGTAGt                                                                                                                                         <  1:390554/141‑1 (MQ=255)
  ataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAg                                                                                                                                          >  3:13670/1‑141 (MQ=255)
  ataGAAGAAACACCACCAACAAATTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTGTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAg                                                                                                                                          <  2:1032706/141‑1 (MQ=255)
    agaagaAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTGCCAATGATTGGTAGTc                                                                                                                                        <  1:544803/141‑1 (MQ=255)
    agaagaAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTc                                                                                                                                        <  3:364676/141‑1 (MQ=255)
    agaagaAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTc                                                                                                                                        <  3:222193/141‑1 (MQ=255)
    agaagaAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTc                                                                                                                                        >  2:385555/1‑141 (MQ=255)
     gaagaaACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTGCAAATGATTGGTAGTCg                                                                                                                                       <  1:34412/141‑1 (MQ=255)
        gaaACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGttt                                                                                                                                    <  3:457567/141‑1 (MQ=255)
         aaaCACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGtttt                                                                                                                                   <  2:368400/141‑1 (MQ=255)
           acacGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATTGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAACGACTACCAATCATTGGCAGTCGTTTTTtg                                                                                                                                <  3:296177/141‑1 (MQ=255)
            cacGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGttttttt                                                                                                                                >  1:424540/1‑140 (MQ=255)
                tCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTc                                                                                                                            <  3:140215/141‑1 (MQ=255)
                tCCAACAATGTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTc                                                                                                                            <  2:511111/141‑1 (MQ=255)
                 ccAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCt                                                                                                                           >  1:463309/1‑141 (MQ=255)
                    acaaTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGa                                                                                                                        <  2:529466/141‑1 (MQ=255)
                    acaaTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGa                                                                                                                        <  1:608287/141‑1 (MQ=255)
                      aaTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGAtt                                                                                                                      >  2:485978/1‑141 (MQ=255)
                       aTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGAttt                                                                                                                     <  1:449743/141‑1 (MQ=255)
                       aTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGAttt                                                                                                                     <  3:355513/141‑1 (MQ=255)
                               agaacTAAATTTATATCAGATATTTAAAGACATTCGTAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACAACAAAAAAAAAAACGACTACCAATCATTGGTAGTCGTTTTTTGTGTCTGGATTTAACCATGa                                                                                                             <  2:949168/141‑1 (MQ=255)
                                 aagTATATTTATATCTGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAAc                                                                                                           <  4:414496/141‑1 (MQ=255)
                                  agTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACa                                                                                                          >  3:258959/1‑141 (MQ=255)
                                      tatTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCACTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAata                                                                                                      <  2:53050/141‑1 (MQ=255)
                                      tatTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCGTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAata                                                                                                      <  2:580305/141‑1 (MQ=255)
                                       atTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAatat                                                                                                     >  1:82913/1‑141 (MQ=255)
                                            tatCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGt                                                                                                >  1:437556/1‑141 (MQ=255)
                                             atCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTa                                                                                               >  2:556446/1‑141 (MQ=255)
                                               cGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACa                                                                                             <  2:693046/141‑1 (MQ=255)
                                               cGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACa                                                                                             >  2:1019913/1‑141 (MQ=255)
                                                 gATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAga                                                                                           <  1:1034633/141‑1 (MQ=255)
                                                    atTTAAAAACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTTGATTTAACCATGAACAAATATGAAGTACAgaatg                                                                                        <  3:38778/141‑1 (MQ=255)
                                                       tAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAgaatgaat                                                                                     >  1:704675/1‑141 (MQ=255)
                                                         aaGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAg                                                                                   >  1:675255/1‑141 (MQ=255)
                                                          aGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGt                                                                                  >  1:380331/1‑141 (MQ=255)
                                                          aGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGt                                                                                  >  3:394392/1‑141 (MQ=255)
                                                          aGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGt                                                                                  >  2:716727/1‑141 (MQ=255)
                                                          aGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGt                                                                                  >  2:974573/1‑141 (MQ=255)
                                                          aGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAAGCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGt                                                                                  <  2:150646/141‑1 (MQ=255)
                                                           gACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTa                                                                                 <  2:407392/141‑1 (MQ=255)
                                                             cATTCATAATATCGCTTTATGTTACTCCGCTTCTCATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATCCAAATATACGAAGGATAGATTGAATAGTAcc                                                                               <  2:273899/141‑1 (MQ=255)
                                                              aTTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCa                                                                              >  1:795616/1‑141 (MQ=255)
                                                               ttCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCAt                                                                             >  4:284212/1‑141 (MQ=255)
                                                               ttCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCAt                                                                             <  1:940632/141‑1 (MQ=255)
                                                                  ataataTCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTaa                                                                          >  2:757580/1‑141 (MQ=255)
                                                                  ataataTCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTaa                                                                          >  3:9481/1‑141 (MQ=255)
                                                                      tatCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAataca                                                                      <  1:532265/141‑1 (MQ=255)
                                                                       atCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacat                                                                     >  4:375738/1‑141 (MQ=255)
                                                                       atCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacat                                                                     >  2:232284/1‑141 (MQ=255)
                                                                        tCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacata                                                                    >  1:996454/1‑141 (MQ=255)
                                                                         cGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacatac                                                                   >  2:618568/1‑141 (MQ=255)
                                                                           cTTTATGTTATTTCGCTTCATATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATacatacat                                                                 >  2:600321/1‑141 (MQ=255)
                                                                            tttATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACAtt                                                                <  1:32818/141‑1 (MQ=255)
                                                                            tttATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACAtt                                                                <  2:800451/141‑1 (MQ=255)
                                                                            tttATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACAtt                                                                >  1:359594/1‑141 (MQ=255)
                                                                              tATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacatccgataa                                                              <  3:23847/141‑8 (MQ=255)
                                                                              tATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTAt                                                              <  1:480354/141‑1 (MQ=255)
                                                                                     tttCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGt                                                       <  1:1002308/141‑1 (MQ=255)
                                                                                     tttCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGt                                                       <  2:342022/141‑1 (MQ=255)
                                                                                      ttCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTa                                                      <  2:1058931/141‑1 (MQ=255)
                                                                                            tcttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTActtctt                                                <  3:407604/141‑1 (MQ=255)
                                                                                              ttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTa                                              <  1:576115/141‑1 (MQ=255)
                                                                                              ttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTa                                              <  2:442963/141‑1 (MQ=255)
                                                                                              ttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTa                                              <  2:770633/141‑1 (MQ=255)
                                                                                              ttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTAGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAAAATGCCTAGTACCATTAATACATACATTATCGGATGTACTTCTTTa                                              <  2:227168/141‑1 (MQ=255)
                                                                                               gagtttATCCTTACTTCGAAGAAGGAAACGACTAGCACTCATTGGCTGTCGTTTTTTGTGTCTGGATTTAACATTGAACAAATATGAAGTACAGAATGAATAGTACTAGTAATACATACAATATAGTAAGTAATGCTTTaa                                             >  4:286810/4‑140 (MQ=255)
                                                                                                 ttttATCCTTACTACAAAGAAAAAAGCGACTACCAATGATTGGTAGTCGTTTTTTGTGTCTGGCTTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGt                                           >  2:922532/1‑141 (MQ=255)
                                                                                                   ttATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGttt                                         <  2:179059/141‑1 (MQ=255)
                                                                                                     aTCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGttttt                                       <  1:211058/141‑1 (MQ=255)
                                                                                                     aTCCTTACGTCAAAGAAAAAAACGACTACCAATCATCGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAACACATACATTATCGGATGTACTTCTTTATGttctt                                       >  4:279444/1‑141 (MQ=255)
                                                                                                        cTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTag                                    >  1:999136/1‑141 (MQ=255)
                                                                                                         ttACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTaga                                   >  2:284068/1‑141 (MQ=255)
                                                                                                         ttACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTaga                                   >  1:660536/1‑141 (MQ=255)
                                                                                                          tACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTagag                                  >  1:271770/1‑141 (MQ=255)
                                                                                                           aCTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAgaga                                 >  2:1062404/1‑141 (MQ=255)
                                                                                                           aCTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAgaga                                 >  2:326698/1‑141 (MQ=255)
                                                                                                                aaaGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGc                            <  1:410136/141‑1 (MQ=255)
                                                                                                                aaaGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGc                            >  1:1077095/1‑141 (MQ=255)
                                                                                                                aaaGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATACTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGc                            >  2:208722/1‑141 (MQ=255)
                                                                                                                aaaGAAAAAAACGACTACCAATCATCGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGc                            <  1:407660/141‑1 (MQ=255)
                                                                                                                  aGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCAt                          >  1:703687/1‑141 (MQ=255)
                                                                                                                   gAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCAtt                         <  4:385297/141‑1 (MQ=255)
                                                                                                                     aaaaaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGt                       <  2:445094/141‑1 (MQ=255)
                                                                                                                      aaaaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTa                      >  3:368362/1‑141 (MQ=255)
                                                                                                                      aaaaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTa                      >  3:17465/1‑141 (MQ=255)
                                                                                                                       aaaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTaa                     >  2:993986/1‑141 (MQ=255)
                                                                                                                        aaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAAt                    <  2:726372/141‑1 (MQ=255)
                                                                                                                          aCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTg                  >  1:1080401/1‑141 (MQ=255)
                                                                                                                          aCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTg                  <  4:173222/141‑1 (MQ=255)
                                                                                                                             aCTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGAt               >  3:301596/1‑141 (MQ=255)
                                                                                                                              ctgccAATGATTGGTAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATa              >  3:232817/4‑141 (MQ=255)
                                                                                                                               tACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAg             >  1:905071/1‑141 (MQ=255)
                                                                                                                                     tgaTTGGTAGTCGTTTTTTTTTTTTGTTTTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTCTCGGATGTACTTCTTTATGTTTTTTACAGATAAGCATTTTAATTGTTTAGAaaata       >  4:407902/3‑141 (MQ=255)
                                                                                                                                     tgaTTGGTAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAaaata       >  1:619210/3‑141 (MQ=255)
                                                                                                                                      cATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATGAATACATATATGATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAaaataa      >  1:214198/1‑141 (MQ=255)
                                                                                                                                        ttGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAaaataaat    >  4:118427/1‑141 (MQ=255)
                                                                                                                                          ggCAGTCGTTTTTTTTGTTTGGTTTTAACCATGAAAAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTTGATAGAAAATAAATcc  >  4:64660/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                          
ATATAGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCC  >  USA300TCH1516_ALE/2372279‑2372557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: