| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,326,827 | A→G | 6.1% | H369H (CAT→CAC) | lacG ← | 6‑phospho‑beta‑galactosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,326,827 | 0 | A | G | 6.1% | 408.4 / 11.9 | 145 | H369H (CAT→CAC) | lacG | 6‑phospho‑beta‑galactosidase |
| Reads supporting (aligned to +/- strand): ref base A (65/70); new base G (2/7); total (68/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
Only 100 of 149 total aligned reads displayed.
TTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCTTTAAGTTGGTATTTAGAACC > USA300TCH1516_ALE/2326687‑2326967 | tttGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCATATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTa > 2:366152/1‑141 (MQ=255) tGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATg > 1:190020/1‑141 (MQ=255) gCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGa < 2:683561/141‑1 (MQ=255) cATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGt < 1:1007939/141‑1 (MQ=255) aTCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGtt < 1:126043/141‑1 (MQ=255) tCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAAGCATTTTCAGTGATATAAATCTTATgatagata > 2:626412/1‑141 (MQ=255) tataATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAgg < 2:71574/141‑1 (MQ=255) taataGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGAt < 2:113547/141‑1 (MQ=255) taataGAATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGAGTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGAt < 2:1080328/141‑1 (MQ=255) ataGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATaa > 1:1019027/1‑141 (MQ=255) aGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATc > 3:435658/1‑141 (MQ=255) aGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATc > 1:887392/1‑141 (MQ=255) gCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCt < 2:1015011/141‑1 (MQ=255) cATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTGTGATAGTTAGGATAATCtt < 1:44337/141‑1 (MQ=255) cTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTaa > 3:435288/1‑141 (MQ=255) tGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGTTAGTTAGGATAATCTTTaac > 3:275529/1‑141 (MQ=255) gCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTaaca > 3:86410/1‑141 (MQ=255) cAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTaacaa > 3:210664/1‑141 (MQ=255) cAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTaacaa > 3:216572/1‑141 (MQ=255) aTAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAacaaca < 4:419094/141‑1 (MQ=255) tAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAacac > 1:168426/1‑141 (MQ=255) cAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGAt < 3:157888/141‑1 (MQ=255) cAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGAt > 2:319001/1‑141 (MQ=255) aaaTGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGAtt > 2:867103/1‑141 (MQ=255) aaTGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGAttt > 1:762449/1‑141 (MQ=255) tgttgtCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGa < 3:425240/141‑1 (MQ=255) tgttgtCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGa < 4:336240/141‑1 (MQ=255) gttgtCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTTTTTTTTTTTAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGAt > 2:1059160/1‑141 (MQ=255) tgtCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCACTTTCAGTGATATAAATCTTATTATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCa > 2:574112/1‑141 (MQ=255) tttACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCatat < 4:341726/140‑1 (MQ=255) cTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTGTGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCatat < 2:623195/141‑1 (MQ=255) cTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACGGGACGCATGATTTGATCatat < 2:545770/141‑1 (MQ=255) cTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCatat < 1:940091/141‑1 (MQ=255) cTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCatat < 2:131702/141‑1 (MQ=255) cTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCatat < 4:220379/141‑1 (MQ=255) cTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCatat < 3:221051/141‑1 (MQ=255) tACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATaa > 3:178716/1‑141 (MQ=255) cATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAAc < 1:91665/141‑1 (MQ=255) aaTCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCtt < 2:333706/141‑1 (MQ=255) cAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAg > 1:1021290/1‑141 (MQ=255) cAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAg > 1:714673/1‑141 (MQ=255) tACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGAt < 4:61767/141‑1 (MQ=255) aCGTGCATCATCATGAACTGTTTTTTCATATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATAATAGTTACGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTCAGGAtt > 2:86292/1‑140 (MQ=255) aCGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATa < 2:69208/141‑1 (MQ=255) gTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGa > 2:421110/1‑141 (MQ=255) tGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGat > 2:591148/1‑141 (MQ=255) ggCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTGGCTTTTGTATAAATCGTATGAGATTTGTGGTAATCGTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGat > 1:270144/2‑141 (MQ=255) gCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGatc < 4:435288/141‑1 (MQ=255) gCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGatc < 3:308533/141‑1 (MQ=255) gCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGatc > 3:454881/1‑141 (MQ=255) catcatcatGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGatca < 2:44293/141‑1 (MQ=255) catGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTGTGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATccagt > 3:338100/1‑141 (MQ=255) tGAACTGCTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATccagtcc > 3:401927/1‑141 (MQ=255) gAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATccagtccc < 1:220112/141‑1 (MQ=255) aCTGTTTTTTTTGTTTTATTAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATccagtcccag > 2:978842/1‑141 (MQ=255) aCTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATccagtcccag < 1:986273/141‑1 (MQ=255) aCTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATccagtcccag < 3:84195/141‑1 (MQ=255) aCTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATccagtcccag < 1:1070960/141‑1 (MQ=255) cTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATccagtcccagt > 3:395805/1‑141 (MQ=255) tGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATccagtcccagtc < 2:140495/141‑1 (MQ=255) ttttttCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGATGATAGATCATCCAGTCCCAGTCtg > 1:553380/1‑141 (MQ=255) ttCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCg < 2:444356/141‑1 (MQ=255) cAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAg > 1:846514/1‑141 (MQ=255) cAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAg < 1:605894/141‑1 (MQ=255) aGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAgg > 2:497495/1‑141 (MQ=255) tCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACg < 2:249165/141‑1 (MQ=255) tCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACg < 2:269714/141‑1 (MQ=255) aTAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCg > 3:150224/1‑141 (MQ=255) aaaTTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGAc < 1:905802/141‑1 (MQ=255) aaTTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACa > 3:285630/1‑141 (MQ=255) aTTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACAt < 2:700055/141‑1 (MQ=255) aTTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACAt < 1:245027/141‑1 (MQ=255) tCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAAt > 3:51385/1‑141 (MQ=255) tttATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTc < 3:136344/141‑1 (MQ=255) tttATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCGTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTc < 2:151305/141‑1 (MQ=255) ttATATCCTAAACCATTTTCAGTGATATAAATCTTGTGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCa < 1:934814/141‑1 (MQ=255) tataTCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCAc < 3:252116/141‑1 (MQ=255) ccTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGt < 1:1035907/141‑1 (MQ=255) aaaCCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCg > 3:104260/1‑141 (MQ=255) ccATTCTCAGTGATATAAAGCTGATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGAACAGACCATCCAGTCCCAGTCTGTGCGAGGTACGCCGACACCAAACTCACGTAGTCCGaca > 2:133312/1‑141 (MQ=255) aTTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGTTAGATCATCCAGTCCCAGTCTGTGCAAGGTAAGTCGACATCAAATTCACGTTGTCCGACAcc < 4:212191/141‑1 (MQ=255) tttCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCGAGTCCCAGTCTGTGCGAGGTACGTCGAAATCAAATTCACGTTGTCCGACACCtt > 2:861186/1‑141 (MQ=255) tttCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCtt < 4:401927/141‑1 (MQ=255) tttCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCtt > 1:886248/1‑141 (MQ=255) gTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCTTTAAGt < 3:26057/141‑1 (MQ=255) tGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCTTTAAGtt > 1:129218/1‑141 (MQ=255) gATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCTTTAAGTTg > 2:9263/1‑141 (MQ=255) gATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCTTTAAGTTg < 3:415404/141‑1 (MQ=255) atatAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCTTTAAGTTgg < 2:144729/141‑1 (MQ=255) tataAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCTTTAAGTTGGt < 3:164220/141‑1 (MQ=255) ataAATCNTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCTTTAAGCTGGTa < 2:814688/141‑1 (MQ=255) aaaTCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCTTTAAGTTGGTAtt < 2:773111/141‑1 (MQ=255) aaaTCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCTTTAAGTTGGTAtt < 4:305118/141‑1 (MQ=255) tCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCTTTAAGTTGGTATTTAg > 1:814703/1‑141 (MQ=255) tCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCTTTAAGTTGGTATTTAg < 1:278717/141‑1 (MQ=255) cTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCTTTAAGTTGGTATTTAGa > 4:179109/1‑141 (MQ=255) ttATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCTTTAAGTTGGTATTTAGaa > 2:296560/1‑141 (MQ=255) tATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCTTTAAGTTGGTATTTAGAAc > 2:359415/1‑141 (MQ=255) aTGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCTTTAAGTTGGTATTTAGAAcc < 1:301641/141‑1 (MQ=255) aTGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCTTTAAGTTGGTATTTAGAAcc > 2:406628/1‑141 (MQ=255) | TTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGGATAATCTTTAACAACACGCATGATTTGATCATATAAACCTTGAGGATAGATCATCCAGTCCCAGTCTGTGCGAGGTACGTCGACATCAAATTCACGTTGTCCGACACCTTTAAGTTGGTATTTAGAACC > USA300TCH1516_ALE/2326687‑2326967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |