Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,284,800 T→C 6.2% N430D (AAT→GAT)  ebh_4 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,284,8000TC 6.2% 418.5 / 10.9 140N430D (AAT→GAT) ebh_4Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base T (69/62);  new base C (4/5);  total (73/67)
Fisher's exact test for biased strand distribution p-value = 7.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

TTTAGTGTGTGTACCACGTGCCGTTGTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGTTAGTTGCTTTCGGTGCTAAAT  >  USA300TCH1516_ALE/2284660‑2284940
                                                                                                                                            |                                                                                                                                            
tttAGTGTGTGTACCACGTGCCGTTGTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAatt                                                                                                                                              >  4:442593/1‑141 (MQ=255)
     tgtgtgtACCACGTGCCGTTGTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTaa                                                                                                                                         <  3:363080/141‑1 (MQ=255)
     tgtgtgtACCACGTGCCGTTGTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTaa                                                                                                                                         <  4:27751/141‑1 (MQ=255)
       tgtgtACCACGTGCCGTTGTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACg                                                                                                                                       <  1:33440/141‑1 (MQ=255)
          gtACCACGTGCCTCTGTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTaa                                                                                                                                    >  2:496382/1‑141 (MQ=255)
            aCCACGTGCCGTTGTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGt                                                                                                                                  <  2:467937/141‑1 (MQ=255)
            aCCACGTGCCGTTGTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGt                                                                                                                                  <  3:59237/141‑1 (MQ=255)
             ccACGTGCCGTTGTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGtt                                                                                                                                 >  2:797738/1‑141 (MQ=255)
               aCGTGCCGTTGTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTac                                                                                                                               >  2:571489/1‑141 (MQ=255)
               aCGTGCAGTTGTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTATCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTac                                                                                                                               >  2:85459/1‑141 (MQ=255)
                cGTGCCGTTGTCCCAACTTGGCTTGTAAATGTAACATTATTTGCAGTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGGCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATCATTAACGTAAGTTaca                                                                                                                              >  2:914396/1‑141 (MQ=255)
                cGTGCCGTTGTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGCATGTTGTAGTTAATGAATTATTAACGTAAGTTaca                                                                                                                              >  3:390767/1‑141 (MQ=255)
                cGTGCCGGTGACGCACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTATGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTaca                                                                                                                              >  2:282908/1‑141 (MQ=255)
                   gCCGTTGTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCt                                                                                                                           <  4:264203/141‑1 (MQ=255)
                   gCCGTTGTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCt                                                                                                                           >  4:439794/1‑141 (MQ=255)
                   gCCGTTGTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCt                                                                                                                           >  2:880744/1‑141 (MQ=255)
                   gCCGATGTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGATGTAGTTAATGAATTATTAACGTAAGTTACACCt                                                                                                                           >  1:1004996/1‑141 (MQ=255)
                      gTTGTCACACCTTGGCTTGTAAATGTCACTTTATTTGCATTTTGATAATAATTAACAGTCATATTTATCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTtgt                                                                                                                        <  1:169200/141‑1 (MQ=255)
                      gTTGTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTtgt                                                                                                                        >  2:955113/1‑141 (MQ=255)
                         gTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGt                                                                                                                     <  2:750572/141‑1 (MQ=255)
                          tCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTa                                                                                                                    <  3:66725/141‑1 (MQ=255)
                          tCACACCTTGGCTTGTAAATGTAACTTTATTTGCAGTTTGATTATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTa                                                                                                                    >  1:934507/1‑141 (MQ=255)
                           cacaCCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTaa                                                                                                                   <  2:195490/141‑1 (MQ=255)
                              acCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGt                                                                                                                <  3:220329/141‑1 (MQ=255)
                               ccTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGtt                                                                                                               <  2:248508/141‑1 (MQ=255)
                               ccTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGtt                                                                                                               >  4:124345/1‑141 (MQ=255)
                                    gCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGt                                                                                                                                  >  2:675730/1‑117 (MQ=255)
                                         tAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTCCATAtt                                                                                                     >  4:345671/1‑141 (MQ=255)
                                           aaTGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTa                                                                                                   >  4:348518/1‑141 (MQ=255)
                                             tGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTaaa                                                                                                 >  1:110561/1‑141 (MQ=255)
                                             tGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATAAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTaaa                                                                                                 <  2:581096/141‑1 (MQ=255)
                                              gTAACTTTATTTGCAGTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATCATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAAt                                                                                                >  1:965894/1‑141 (MQ=255)
                                                aaCTTTATTTGCAGTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATCATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTg                                                                                              <  2:319043/141‑1 (MQ=255)
                                                 aCTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGtt                                                                                                                                 <  4:289095/105‑1 (MQ=255)
                                                 aCTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGtt                                                                                                                                 >  3:289095/1‑105 (MQ=255)
                                                  cTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGAt                                                                                            <  1:265463/141‑1 (MQ=255)
                                                   tttATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATc                                                                                           >  1:114716/1‑141 (MQ=255)
                                                   tttATTTGCAGTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATCATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATc                                                                                           >  2:764832/1‑141 (MQ=255)
                                                    ttATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCt                                                                                          >  2:558391/1‑141 (MQ=255)
                                                      aTTTGCAGTTTTATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAAGGAATGTTGTCGTTAATGAATCATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGc                                                                                        <  1:377738/141‑1 (MQ=255)
                                                      aTTTGCAGTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATCATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGc                                                                                        <  3:165257/141‑1 (MQ=255)
                                                       tttGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCt                                                                                       <  3:440067/141‑1 (MQ=255)
                                                       tttGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCt                                                                                       >  1:390718/1‑141 (MQ=255)
                                                         tGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCttt                                                                                     >  4:24342/1‑141 (MQ=255)
                                                          gCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCtttt                                                                                    <  4:201267/141‑1 (MQ=255)
                                                           cATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTa                                                                                   <  2:1000032/141‑1 (MQ=255)
                                                           cATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTa                                                                                   <  2:1000261/141‑1 (MQ=255)
                                                               ttGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAAAGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTaaaaa                                                                               <  2:885685/141‑4 (MQ=255)
                                                                 gATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAg                                                                             >  1:221180/1‑141 (MQ=255)
                                                                 gATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAg                                                                             >  1:636747/1‑141 (MQ=255)
                                                                     aTAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCAcc                                                                         <  4:385202/141‑1 (MQ=255)
                                                                       aaTTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCaa                                                                       >  1:287593/1‑141 (MQ=255)
                                                                         ttAACAGTCATATTTTTCACTCCTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATCATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCaaaa                                                                     <  1:429206/141‑1 (MQ=255)
                                                                          tAACAGTCATATTGTTCTATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAAt                                                                    <  2:205311/141‑1 (MQ=255)
                                                                             cAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAGATTGATCTGCTTTTAATGAAGCACCAAAATTAc                                                                 >  1:92495/1‑141 (MQ=255)
                                                                              aGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTAcc                                                                >  2:978221/1‑141 (MQ=255)
                                                                               ttCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCa                                                               <  2:551468/140‑1 (MQ=255)
                                                                                tCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCAt                                                              >  1:729689/1‑141 (MQ=255)
                                                                                tCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCAt                                                              >  1:586556/1‑141 (MQ=255)
                                                                                   tatTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGt                                                           <  4:182115/141‑1 (MQ=255)
                                                                                    atTTTTCATTACTTTTCTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGtt                                                          >  2:556000/1‑141 (MQ=255)
                                                                                     tttttCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTa                                                         <  1:616030/141‑1 (MQ=255)
                                                                                     tttttCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTa                                                         <  1:978135/141‑1 (MQ=255)
                                                                                     tttttCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTa                                                         >  2:1042888/1‑141 (MQ=255)
                                                                                          cAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGAt                                                    <  3:112233/141‑1 (MQ=255)
                                                                                             tactgtactgTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTc                                                 <  1:1038623/141‑1 (MQ=255)
                                                                                             tactgtactgTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTc                                                 >  2:852545/1‑141 (MQ=255)
                                                                                              tctgttctgtCTTCATTACCATTCGGGAATGTTGTCGTTAATGAATTATTAACTTAAGTTACACCTTGTGTTAATGTTACTTTATATTTAAATTGATCTGCTTTTAATGAAGCATCAAAATTACCATAGTTACCGATTTCa                                                >  2:299012/2‑141 (MQ=255)
                                                                                                   actgtCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTAtt                                           <  2:338029/141‑1 (MQ=255)
                                                                                                    ctgtCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTa                                          >  2:326020/1‑141 (MQ=255)
                                                                                                    ctgtCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTCTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTa                                          >  1:854843/1‑141 (MQ=255)
                                                                                                      gtCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTTCTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAAt                                        >  2:625671/1‑141 (MQ=255)
                                                                                                      gtCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAAt                                        >  4:324973/1‑141 (MQ=255)
                                                                                                        cTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAg                                      <  3:305873/141‑1 (MQ=255)
                                                                                                         ttCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGt                                     >  2:621509/1‑141 (MQ=255)
                                                                                                            aTTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTCCCGATTTCAGTATTAATAGtata                                  <  1:1068349/141‑1 (MQ=255)
                                                                                                            aTTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGtata                                  <  3:428999/141‑1 (MQ=255)
                                                                                                              tACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATAtt                                <  1:92095/141‑1 (MQ=255)
                                                                                                              tACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATAtt                                >  4:322360/1‑141 (MQ=255)
                                                                                                              tACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATAtt                                >  2:206811/1‑141 (MQ=255)
                                                                                                                ccATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCt                              >  3:46719/1‑141 (MQ=255)
                                                                                                                ccATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCt                              <  1:599203/141‑1 (MQ=255)
                                                                                                                ccATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCt                              >  2:83175/1‑141 (MQ=255)
                                                                                                                       ggAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGt                       <  2:507340/141‑1 (MQ=255)
                                                                                                                         aaTGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGTTa                     <  2:738332/141‑1 (MQ=255)
                                                                                                                         aaTGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGTTa                     >  2:7398/1‑141 (MQ=255)
                                                                                                                          aTGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGTTAg                    >  2:474312/1‑141 (MQ=255)
                                                                                                                            gttgtAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGTTAGtt                  <  2:194992/141‑1 (MQ=255)
                                                                                                                            gttgtAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGTTAGtt                  <  3:90623/141‑1 (MQ=255)
                                                                                                                               gtAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGTTAGTTGCt               >  4:373597/1‑141 (MQ=255)
                                                                                                                                tAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGTTAGTTGCtt              >  2:13238/1‑141 (MQ=255)
                                                                                                                                 aGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGTTAGTTGCttt             >  1:189255/1‑141 (MQ=255)
                                                                                                                                 aGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGTTAGTTGCttt             >  1:142886/1‑141 (MQ=255)
                                                                                                                                  gTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGTTAGTTGCTTTc            >  4:249592/1‑141 (MQ=255)
                                                                                                                                     aaTGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGTTAGTTGCTTTCGGt         >  2:421710/1‑141 (MQ=255)
                                                                                                                                         aaTTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGTTAGTTGCTTTCGGTGCTa     <  1:326020/141‑1 (MQ=255)
                                                                                                                                          attattAACGTAAGTTACACCTTGTGGTAATGTTACTTCCTATTGAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGTTAGTTGCTTTCGGTGCTaa    >  1:792635/1‑141 (MQ=255)
                                                                                                                                            tattaACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGTTAGTTGCTTTCGGTGCTAAAt  <  1:734200/141‑1 (MQ=255)
                                                                                                                                            tattaACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGTTAGTTGCTTTCGGTGCTAAAt  >  2:701948/1‑141 (MQ=255)
                                                                                                                                            tattaACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATAACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGTTAGTTGCTTTCGGTGCTAAAt  <  2:802607/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TTTAGTGTGTGTACCACGTGCCGTTGTCACACCTTGGCTTGTAAATGTAACTTTATTTGCATTTTGATCATAATTAACAGTCATATTTTTCAATACTGTACTGTCTTCATTACCATTAGGGAATGTTGTAGTTAATGAATTATTAACGTAAGTTACACCTTGTGGTAATGTTACTTCATATTTAAATTGATCTGCTTTTAATGAAGCACCAAAATTACCATTGTTACCGATTTCAGTATTAATAGTATATTCTTTATTGTTAGTTGCTTTCGGTGCTAAAT  >  USA300TCH1516_ALE/2284660‑2284940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: