BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,120,664	G→A	7.5%	D37D (GAC→GAT)	USA300HOU_RS10785 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,120,664	0	G	A	7.5%	361.9 / 13.8	119	D37D (GAC→GAT)	USA300HOU_RS10785	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (63/47); new base A (6/3); total (69/50)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01

Only 100 of 120 total aligned reads displayed.

ATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTAT  >  USA300TCH1516_ALE/2120524‑2120802

aTGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATgg                                                                                                                                            >  2:587370/1‑141 (MQ=255)

   aCTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCt                                                                                                                                         <  1:592804/141‑1 (MQ=255)

   aCTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCt                                                                                                                                         <  2:1076496/141‑1 (MQ=38)

     tAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAg                                                                                                                                       <  4:117197/141‑1 (MQ=255)

       aGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGaa                                                                                                                                     <  1:503076/141‑1 (MQ=255)

        gttaaTTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAaac                                                                                                                                    >  2:1080817/5‑141 (MQ=38)

          tCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCGTAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGaacaa                                                                                                                                  <  1:852966/141‑1 (MQ=255)

               aGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCGGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCAtt                                                                                                                             <  1:281820/141‑1 (MQ=255)

                gTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCAttt                                                                                                                            >  1:872486/1‑141 (MQ=255)

                    gTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGAc                                                                                                                        <  3:286398/141‑1 (MQ=255)

                     tCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACg                                                                                                                       >  3:273068/1‑141 (MQ=255)

                      ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGAcac                                                                                                                      >  2:705909/1‑139 (MQ=255)

                      ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGc                                                                                                                      >  2:760113/1‑141 (MQ=255)

                      ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGc                                                                                                                      >  1:663286/1‑141 (MQ=255)

                          ttCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCtata                                                                                                                  >  2:538008/1‑141 (MQ=255)

                          ttCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCtata                                                                                                                  >  1:644608/1‑141 (MQ=255)

                           tCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACtatat                                                                                                                 >  1:734959/1‑140 (MQ=255)

                           tCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATAc                                                                                                                 <  2:1003342/141‑1 (MQ=255)

                               cTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTc                                                                                                             <  1:429292/141‑1 (MQ=255)

                               cTGTGTTATTTTCAATCCAATCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTc                                                                                                             <  3:179267/141‑1 (MQ=255)

                                 gtgtTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCgc                                                                                                           <  1:455632/141‑2 (MQ=255)

                                    ttATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTc                                                                                                        <  2:629719/141‑1 (MQ=255)

                                      aTTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCtt                                                                                                      <  1:299598/141‑1 (MQ=255)

                                       ttttCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTa                                                                                                     <  4:322472/141‑1 (MQ=255)

                                           cAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAActc                                                                                                 >  1:372840/1‑141 (MQ=255)

                                            aaTCCATTCATACACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAActct                                                                                                <  3:321195/141‑1 (MQ=255)

                                            aaTCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCGCTTCTTAActct                                                                                                <  2:82870/141‑1 (MQ=255)

                                             aTCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCtt                                                                                               <  1:846708/141‑1 (MQ=255)

                                              tCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTa                                                                                              >  1:475000/1‑141 (MQ=255)

                                              tCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTa                                                                                              >  1:992887/1‑141 (MQ=255)

                                                  ttCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTg                                                                                          <  1:641314/141‑1 (MQ=255)

                                                   tCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGa                                                                                         >  4:147125/1‑141 (MQ=255)

                                                   tCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGa                                                                                         <  3:281012/141‑1 (MQ=255)

                                                      tAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGaaaa                                                                                      >  2:1046637/1‑141 (MQ=255)

                                                        aaCACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATc                                                                                    <  2:730749/141‑1 (MQ=255)

                                                          cacTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGGTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCaa                                                                                  >  1:432525/1‑141 (MQ=255)

                                                              ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                                                              >  1:1050983/1‑141 (MQ=255)

                                                              ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                                                              >  2:1054135/1‑141 (MQ=255)

                                                              ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                                                              >  2:101574/1‑141 (MQ=255)

                                                              ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                                                              >  1:940092/1‑141 (MQ=255)

                                                              ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                                                              >  2:944847/1‑141 (MQ=255)

                                                                gTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCAt                                                                            <  1:929619/141‑1 (MQ=255)

                                                                gTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCAt                                                                            >  3:271611/1‑141 (MQ=255)

                                                                        atatCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACatta                                                                    <  2:669992/141‑1 (MQ=255)

                                                                        atatCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACatta                                                                    <  1:460266/141‑1 (MQ=255)

                                                                            cTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACattaatta                                                                >  4:190496/1‑141 (MQ=255)

                                                                            cTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACattaatta                                                                <  4:228471/141‑1 (MQ=255)

                                                                              aaaGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAattatt                                                              <  1:522513/141‑1 (MQ=255)

                                                                               aaGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTAttt                                                             >  2:1058932/1‑141 (MQ=255)

                                                                                aGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTAtttt                                                            <  1:576778/141‑1 (MQ=255)

                                                                                 gCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTAttttt                                                           >  1:712845/1‑141 (MQ=255)

                                                                                    gtgtATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGa                                                        >  4:128022/1‑141 (MQ=255)

                                                                                    gtgtATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGa                                                        >  2:409349/1‑141 (MQ=255)

                                                                                           gATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGt                                                 <  4:264319/141‑1 (MQ=255)

                                                                                               gTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAcc                                             <  1:617052/141‑1 (MQ=255)

                                                                                                ttCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAccc                                            >  2:60905/1‑141 (MQ=255)

                                                                                                 tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCTTTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCACAGTCTTTTACATTAATTATTTTTCGACATACGGCACCCg                                           <  4:174304/141‑1 (MQ=255)

                                                                                                 tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCg                                           >  2:883004/1‑141 (MQ=255)

                                                                                                 tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCg                                           >  2:927714/1‑141 (MQ=255)

                                                                                                 tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCg                                           >  2:625438/1‑141 (MQ=255)

                                                                                                  ctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGt                                          >  2:723508/1‑141 (MQ=255)

                                                                                                  ctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGt                                          >  2:723512/1‑141 (MQ=255)

                                                                                                   tcgtcaGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCGCTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGtt                                         >  1:22892/4‑141 (MQ=255)

                                                                                                    catcaGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGttt                                        >  4:421057/1‑141 (MQ=255)

                                                                                                     atcaGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGtttt                                       >  1:29322/1‑141 (MQ=255)

                                                                                                       caGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGt                                     <  1:34641/141‑1 (MQ=255)

                                                                                                        aGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCTTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTa                                    >  1:340122/1‑141 (MQ=255)

                                                                                                         gAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTaa                                   <  2:379579/141‑1 (MQ=255)

                                                                                                           aaCTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATa                                 <  4:248214/141‑1 (MQ=255)

                                                                                                              tagtagCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCGCTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGTTTTGTAATACAt                              >  1:715115/1‑141 (MQ=255)

                                                                                                              tAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAcc                                             <  1:1074934/126‑1 (MQ=255)

                                                                                                              tAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAcc                                             >  2:1074934/1‑126 (MQ=255)

                                                                                                               aGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACAtt                             >  1:1014721/1‑141 (MQ=255)

                                                                                                               aGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACAtt                             <  1:923004/141‑1 (MQ=255)

                                                                                                               aGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACAtt                             >  2:993461/1‑141 (MQ=255)

                                                                                                               aGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACAtt                             >  3:57508/1‑141 (MQ=255)

                                                                                                                gTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTg                            >  2:176986/1‑141 (MQ=255)

                                                                                                                  aaCCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGtt                          >  1:26639/1‑141 (MQ=255)

                                                                                                                  aaCCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGtt                          >  3:427037/1‑141 (MQ=255)

                                                                                                                       gTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGt                     >  1:572862/1‑141 (MQ=255)

                                                                                                                       gTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGt                     >  1:789354/1‑141 (MQ=255)

                                                                                                                         cAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcc                   >  2:400888/1‑141 (MQ=255)

                                                                                                                          aaTTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcct                  >  2:780143/1‑141 (MQ=255)

                                                                                                                           aTTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcctc                 <  3:403180/141‑1 (MQ=255)

                                                                                                                           aTTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATAAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcctc                 <  1:590673/141‑1 (MQ=255)

                                                                                                                            ttGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcctcc                <  1:635701/141‑1 (MQ=255)

                                                                                                                             tGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTcctcct               <  2:205492/141‑1 (MQ=255)

                                                                                                                              gAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCtt              >  2:114196/1‑141 (MQ=255)

                                                                                                                               aaTTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTg             >  1:1084372/1‑141 (MQ=255)

                                                                                                                                 tttCCTCCCTAGTCTAGAACAACCATTTCGACGCAATACGCTCGCTTCTTAACTCTTAATTGAAAAGCAAAGCCATGTACCTTAGTTCTTTTTCGCCATTCGTCACCCGTTTCGTAACGCATTGTTCTCGTCCTCCTTGTc           <  2:777534/141‑1 (MQ=255)

                                                                                                                                  ttCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGGCATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCa          <  1:842338/141‑1 (MQ=255)

                                                                                                                                  ttCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCa          <  3:124859/141‑1 (MQ=255)

                                                                                                                                   tCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCCTCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTATTACATTGTTCTCGTCCTCCTTGTCAt         >  1:217886/1‑141 (MQ=255)

                                                                                                                                   tCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCAt         >  1:962909/1‑141 (MQ=255)

                                                                                                                                    ccTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTGATACATTGTTCTCGTCCTCCTTGTCAtt        >  1:755978/1‑141 (MQ=255)

                                                                                                                                        cATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAACATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTAGTGTAGTACAGTGTTCCTGACGTCTTTGTCATTATCt    >  2:271327/1‑141 (MQ=255)

                                                                                                                                        cATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCt    <  1:766597/141‑1 (MQ=255)

                                                                                                                                        cATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCt    >  4:290744/1‑141 (MQ=255)

                                                                                                                                          tGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCtat  >  1:861688/1‑141 (MQ=255)

                                                                                                                                          tGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCtat  >  4:229346/1‑141 (MQ=255)

ATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTAT  >  USA300TCH1516_ALE/2120524‑2120802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`