BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,110,686	C→T	5.6%	V23V (GTG→GTA)	USA300HOU_RS10685 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,110,686	0	C	T	5.6%	375.7 / 8.4	124	V23V (GTG→GTA)	USA300HOU_RS10685	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (59/58); new base T (5/2); total (64/60)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.08e-01

Only 100 of 128 total aligned reads displayed.

TTTAAGTCATGAGCAAACTCCGGATGATGTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTA  >  USA300TCH1516_ALE/2110546‑2110826

tttAAGTCATGAGCAAACTCCGGATGATGTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGc                                                                                                                                              <  1:806934/141‑1 (MQ=255)

    aGTCATGAGCAAACTCCGGATGATGTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCActc                                                                                                                                          <  2:1048323/141‑1 (MQ=255)

     gTCATGAGCAAACTCCGGATGATGTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCActct                                                                                                                                         <  1:30898/141‑1 (MQ=255)

      tCATGAGCAAACTCCGGATGATGTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCtt                                                                                                                                        <  4:337289/141‑1 (MQ=255)

       cATGAGCAAACTCCTGATGATGTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGCCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCttt                                                                                                                                       <  2:1018754/141‑1 (MQ=255)

         tGAGCAATCTCCGGATGATGTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCttttt                                                                                                                                     <  2:775423/141‑1 (MQ=255)

         tGAGCAAACTCCGGATGATGTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCttttt                                                                                                                                     <  2:665916/141‑1 (MQ=255)

           aGCAAAATCCGGATGATGTTCTAGCGATAATATGTGATCTACATCCAACGACTTACGCTTACTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTGGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCt                                                                                                                                   >  4:376217/1‑141 (MQ=255)

             cAAACTCCGGATGATGTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCttg                                                                                                                                 <  2:845162/141‑1 (MQ=255)

              aaaCTCCGGATGATGTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCTTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCttgt                                                                                                                                <  4:447977/141‑1 (MQ=255)

                 cTCCGGATGATGTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTtgttgt                                                                                                                             >  3:80514/1‑141 (MQ=255)

                   ccGGATGATGTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGttt                                                                                                                           >  3:358685/1‑141 (MQ=255)

                   ccGGATGATGTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGttt                                                                                                                           <  1:970529/141‑1 (MQ=255)

                    cGGATGATGTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTg                                                                                                                          >  1:802619/1‑141 (MQ=255)

                     ggATGATGTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGc                                                                                                                         <  1:777020/141‑1 (MQ=255)

                     ggATGATGTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGc                                                                                                                         <  3:290599/141‑1 (MQ=255)

                      gatgatGTTCTAGCGATAATATATGATCTACATCCAACGACTTATGTTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGTACTCTTTTTCTTGTTGTTTGcc                                                                                                                        >  2:837062/1‑141 (MQ=255)

                       atgatgTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACGTTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTCGCCa                                                                                                                       <  2:331468/141‑1 (MQ=255)

                          atgTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTc                                                                                                                    <  1:277104/141‑1 (MQ=255)

                            gTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCtt                                                                                                                  <  2:1037459/141‑1 (MQ=255)

                              tCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTa                                                                                                                >  1:189366/1‑141 (MQ=255)

                               cTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAg                                                                                                               <  3:290156/141‑1 (MQ=255)

                                  gCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACt                                                                                                            <  1:185916/141‑1 (MQ=255)

                                     ataataTATGATCTACATCCAACGACTTATGTTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGTACTCTTTTTCTTGTTTTTTGCCATTCTTTAGACTtat                                                                                                         >  2:178135/1‑141 (MQ=255)

                                     ataataTATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTtat                                                                                                         <  4:41112/141‑1 (MQ=255)

                                      taataTATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTtata                                                                                                        <  4:291217/141‑1 (MQ=255)

                                      taataTATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTtata                                                                                                        >  4:34439/1‑141 (MQ=255)

                                      taataTATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTtata                                                                                                        >  1:625255/1‑141 (MQ=255)

                                      taataTATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTtata                                                                                                        >  4:148722/1‑141 (MQ=255)

                                      taataTATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTtata                                                                                                        >  4:47177/1‑141 (MQ=255)

                                       aataTATGATCTACATCCAACGACTTTCGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAg                                                                                                       <  1:553793/141‑1 (MQ=255)

                                        atatatGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGa                                                                                                      >  1:747081/1‑141 (MQ=255)

                                        atatatGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGa                                                                                                      >  3:27779/1‑141 (MQ=255)

                                         tatatGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTGGCCGTCTCTCTTACATTTTGGGCATTCATAATTATCGCTTGCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGaa                                                                                                     >  3:42729/1‑141 (MQ=255)

                                          atatGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTTTTGTTTGCCACTCTTTAGACTTATAGAAt                                                                                                    >  2:519903/1‑141 (MQ=255)

                                           tatGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATc                                                                                                   >  2:977683/1‑141 (MQ=255)

                                            atGATCTACATCCAACGACTTACGCTTGCTGTTTTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAGTTATCTCTTTCTAGCAATCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCg                                                                                                  <  4:232839/141‑1 (MQ=255)

                                                tCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATg                                                                                              >  4:431619/1‑141 (MQ=255)

                                                   aCATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGt                                                                                           >  4:191836/1‑141 (MQ=255)

                                                       ccAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGt                                                                                       >  2:18372/1‑141 (MQ=255)

                                                        cAACGGCTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTc                                                                                      <  4:134538/141‑1 (MQ=255)

                                                                tatgtttgcttTTGTCATATGTCGTTAACTTGCGGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGTACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATACGTTCGTCTTTAGtca                                                                              >  1:561478/6‑141 (MQ=255)

                                                                tatgtttgcttTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGTACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATACGTTCGTCTTTAGtca                                                                              >  1:1034043/6‑141 (MQ=255)

                                                                  cGCTTTCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGtcatc                                                                            <  2:28452/141‑1 (MQ=255)

                                                                  cGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGtcatc                                                                            >  3:164987/1‑141 (MQ=255)

                                                                  cGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGtcatc                                                                            >  1:1085776/1‑141 (MQ=255)

                                                                    cttgcttTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTcatcat                                                                          <  1:21008/141‑1 (MQ=255)

                                                                     ttgcttTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTATTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATa                                                                         <  4:402381/141‑1 (MQ=255)

                                                                       gcttTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAAt                                                                       <  1:234228/141‑1 (MQ=255)

                                                                          tttGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGtt                                                                    >  4:277360/1‑141 (MQ=255)

                                                                           ttGTCATATGTCGTTAACTTGCCTTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGttt                                                                   <  2:277902/141‑1 (MQ=255)

                                                                           ttGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGttt                                                                   <  2:658703/141‑1 (MQ=255)

                                                                             gTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATACTTATCTCTTTCTAGCGCTATTTTTCTTGTTGTTGGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCa                                                                 <  3:244797/141‑1 (MQ=255)

                                                                              taatatGACGTTAACTTTCCTTCTGTATTACTTTTTTAACATTCATTATTATCTCTTTCTATCGCTCTTTTTTTTGTTGTTTGCTATGCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCAc                                                                <  3:153469/139‑1 (MQ=255)

                                                                              tCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCAc                                                                >  2:671672/1‑141 (MQ=255)

                                                                              tCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCAc                                                                >  3:375983/1‑141 (MQ=255)

                                                                               cATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCAcc                                                               <  2:129095/141‑1 (MQ=255)

                                                                                         tAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACt                                                     >  1:751584/1‑141 (MQ=255)

                                                                                         tAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCAGTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACt                                                     >  3:291519/1‑141 (MQ=255)

                                                                                           aCTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTa                                                   <  4:361183/141‑1 (MQ=255)

                                                                                           aCTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTa                                                   <  2:290721/141‑1 (MQ=255)

                                                                                            cTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTaa                                                  >  2:144563/1‑141 (MQ=255)

                                                                                             ttGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAag                                                 <  1:818509/141‑1 (MQ=255)

                                                                                             ttGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTAATCATAATGTTTCACCTTATATAACTTAag                                                 >  3:434581/1‑141 (MQ=255)

                                                                                              tGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAagt                                                >  2:196767/1‑141 (MQ=255)

                                                                                                ccGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATATAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAagtag                                              >  1:737520/1‑141 (MQ=255)

                                                                                                ccGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAagtag                                              >  1:675810/1‑141 (MQ=255)

                                                                                                    ctctctTACATTGTTGACATTCATCATTATCTATTTCTAGCACTCTTTTTCTTGTTGTTAGCAATTATTAAGACTTCTAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATc                                          <  1:542177/141‑1 (MQ=255)

                                                                                                     tctctTACATTGTTGACATTCATAATTATCTCTTTCTAGTACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATACGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCa                                         <  1:654429/141‑1 (MQ=255)

                                                                                                     tctctTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCa                                         <  2:141345/141‑1 (MQ=255)

                                                                                                      ctctTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCaa                                        >  2:787294/1‑141 (MQ=255)

                                                                                                         ttACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGAc                                     >  2:903821/1‑141 (MQ=255)

                                                                                                          tACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACg                                    <  2:485115/141‑1 (MQ=255)

                                                                                                          tACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACg                                    <  1:256077/141‑1 (MQ=255)

                                                                                                                gttgACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCta                              >  2:752090/1‑141 (MQ=255)

                                                                                                                 ttgACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGtt                                                                                          <  2:109583/80‑1 (MQ=255)

                                                                                                                 ttgACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGtt                                                                                          >  1:109583/1‑80 (MQ=255)

                                                                                                                  tgACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCtata                            >  2:687251/1‑141 (MQ=255)

                                                                                                                    aCATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACt                          >  1:688672/1‑141 (MQ=255)

                                                                                                                        tCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGAt                      <  2:620170/141‑1 (MQ=255)

                                                                                                                         cATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCGTCTATACTTGAtg                     <  4:384730/141‑1 (MQ=255)

                                                                                                                         cATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGAtg                     >  3:342047/1‑141 (MQ=255)

                                                                                                                           tAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGAtgtg                   >  2:1050680/1‑141 (MQ=255)

                                                                                                                           tAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGAtgtg                   <  1:244546/141‑1 (MQ=255)

                                                                                                                            aaTTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATgtgt                  >  1:218253/1‑141 (MQ=255)

                                                                                                                             aTTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTgta                 <  3:387626/141‑1 (MQ=255)

                                                                                                                              ttATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTgtag                <  4:4807/141‑1 (MQ=255)

                                                                                                                               tATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTgtagt               >  4:231816/1‑141 (MQ=255)

                                                                                                                               tATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTgtagt               >  2:834486/1‑141 (MQ=255)

                                                                                                                               tATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTgtagt               >  4:51776/1‑141 (MQ=255)

                                                                                                                                aTCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTgtagta              >  1:676676/1‑141 (MQ=255)

                                                                                                                                 tctcTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTaa             <  1:34243/141‑1 (MQ=255)

                                                                                                                                   tctTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATg           >  1:94169/1‑141 (MQ=255)

                                                                                                                                   tctTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATg           >  1:1015532/1‑141 (MQ=255)

                                                                                                                                   tctTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATg           >  2:721332/1‑141 (MQ=255)

                                                                                                                                      ttCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTAt        <  3:323439/141‑1 (MQ=255)

                                                                                                                                          aGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTAc    >  2:1049158/1‑141 (MQ=255)

                                                                                                                                           ccACTCTTTTTCTTGTTGTTTGCCATTCTTTATACTTAAAGAATCTTATGCGTTCGTTTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTATTATCAAGACTTATCTATACTTTATGTGTAGTAATGTATTTACt   <  4:244237/140‑1 (MQ=255)

                                                                                                                                            cACTCTTTTTTTTTTTTTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTa  >  2:8131/1‑141 (MQ=255)

                                                                                                                                            cACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTa  >  3:102359/1‑141 (MQ=255)

TTTAAGTCATGAGCAAACTCCGGATGATGTTCTAGCGATAATATATGATCTACATCCAACGACTTACGCTTGCTTTTGTCATATGTCGTTAACTTGCCGTCTCTCTTACATTGTTGACATTCATAATTATCTCTTTCTAGCACTCTTTTTCTTGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTA  >  USA300TCH1516_ALE/2110546‑2110826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`