BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,997,022	G→C	6.2%	noncoding (52/93 nt)	USA300TCH1516_01869 ←	tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,997,022	0	G	C	6.2%	384.1 / 13.2	122	noncoding (52/93 nt)	USA300TCH1516_01869	tRNA‑Ser
Reads supporting (aligned to +/- strand): ref base G (43/71); new base C (5/3); total (48/74)
Fisher's exact test for biased strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01

Only 100 of 122 total aligned reads displayed.

CTATAAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGTCTCGACGGGAATCGAACCCGCGATCTCCTGCGTGACAGGC  >  USA300TCH1516_ALE/1996883‑1997158

cTATAAAATATTGCGGGAGGCGGATTTGAACCCCCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGc                                                                                                                                         <  2:788675/141‑1 (MQ=255)

cTATAAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGc                                                                                                                                         <  1:247355/141‑1 (MQ=255)

 tataAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGcc                                                                                                                                        <  3:394936/141‑1 (MQ=255)

 tataAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGcc                                                                                                                                        >  2:836417/1‑141 (MQ=255)

 tataAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGcc                                                                                                                                        >  1:743008/1‑141 (MQ=255)

   taAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTCAAGGCCCt                                                                                                                                      >  2:1032454/1‑141 (MQ=255)

    aaaaTATTGCGGGAGGCGGATTTGAACCACCGCCATTCGGGTTATGAGCCCGACGAGCTACCGAACGGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTg                                                                                                                                     <  4:300688/141‑1 (MQ=255)

    aaaaTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTg                                                                                                                                     <  1:326345/141‑1 (MQ=255)

    aaaaTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTg                                                                                                                                     >  1:611747/1‑141 (MQ=255)

      aaTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTc                                                                                                                                   <  3:90983/141‑1 (MQ=255)

         atTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGt                                                                                                                                >  3:410994/1‑141 (MQ=255)

         atTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGt                                                                                                                                >  4:143293/1‑141 (MQ=255)

          ttGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGtt                                                                                                                               <  1:72584/141‑1 (MQ=255)

          ttGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGtt                                                                                                                               >  3:72184/1‑141 (MQ=255)

           tGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCggagt                                                                                                                              >  4:144772/1‑138 (MQ=255)

           tGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGttt                                                                                                                              >  1:60018/1‑141 (MQ=255)

           tGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGACGGctt                                                                                                                              >  1:80145/1‑141 (MQ=255)

            gCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGtttt                                                                                                                             >  2:435653/1‑141 (MQ=255)

            gCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGtttt                                                                                                                             >  1:103548/1‑141 (MQ=255)

             cGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTc                                                                                                                            <  4:123639/141‑1 (MQ=255)

              gggAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCa                                                                                                                           <  2:931510/141‑1 (MQ=255)

                gAGGCGGAGTTGAACCACCGGCCTTCGGGTTATGAGCCCGACGAGCCACCGGGCTGCTCCATCCCGCTATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTGAGGCCCTGTCGGTTGTCAAg                                                                                                                         <  2:752298/141‑1 (MQ=37)

                  ggCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCAGCGATACTCCAAAATAATGGCGGAGGCAGAGGGATTCGAACCCCAGCGGCAAGTTAAGGCCCTGTCGGTTTTCAAGAc                                                                                                                       <  1:432395/141‑1 (MQ=25)

                     ggATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGa                                                                                                                    >  4:119265/1‑141 (MQ=255)

                        tttGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATcc                                                                                                                 <  1:810456/141‑1 (MQ=255)

                        tttGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATcc                                                                                                                 >  3:303292/1‑141 (MQ=255)

                        tttGAACCACCGACCTTCATCGGGTGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATcc                                                                                                                 <  1:826289/141‑1 (MQ=37)

                        ttggAACCACCCACCTTCGGGTTAGGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATcc                                                                                                                 <  2:650327/138‑1 (MQ=255)

                          tGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCt                                                                                                               <  1:654525/141‑1 (MQ=255)

                            aaCCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTc                                                                                                             >  3:196092/1‑141 (MQ=255)

                             accaccGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCa                                                                                                            <  3:180798/141‑1 (MQ=255)

                               caccGCCCTTCGGTTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCTCGATAATAAAAAATAATGGCGGAGGAAGTGGTGTTCGAACCCCCGCTGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGc                                                                                                          <  2:38021/141‑1 (MQ=37)

                               caccGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATcc                                                                                                                 >  2:116370/1‑134 (MQ=255)

                               caccGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATcc                                                                                                                 <  1:116370/134‑1 (MQ=255)

                               caccGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGc                                                                                                          <  2:878613/141‑1 (MQ=255)

                               caccGACCCCCGGGTTACGAGCCCGACGGGCTACCGAACTGCCCCATCCCGCGATAGTACAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGc                                                                                                          <  2:60018/141‑1 (MQ=18)

                                accGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCATCAGcg                                                                                                         >  4:353539/1‑140 (MQ=255)

                                  cGACCGTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTACAAACAGCAATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCgg                                                                                                       <  2:493725/141‑1 (MQ=25)

                                    aCCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGAc                                                                                                     <  1:250867/141‑1 (MQ=255)

                                    aCCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGAc                                                                                                     >  4:162331/1‑141 (MQ=255)

                                    aCCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGAc                                                                                                     >  2:558100/1‑141 (MQ=255)

                                       ttggggTGATGAGCCCGACCAGCTACCGAACTGCTCCATCGCGCAATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGNACCCNCGCGGCCNGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTg                                                                                                  <  4:372326/138‑1 (MQ=21)

                                       ttCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCTATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTg                                                                                                  <  1:556841/141‑1 (MQ=255)

                                       ttCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTg                                                                                                  <  2:288749/141‑1 (MQ=255)

                                           ggTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTa                                                                                              <  2:178279/141‑1 (MQ=255)

                                            gTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTAt                                                                                             <  4:168370/141‑1 (MQ=255)

                                              tATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATtc                                                                                           <  2:819647/141‑1 (MQ=255)

                                               aTGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCt                                                                                                                                      >  3:380623/1‑97 (MQ=255)

                                               aTGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCt                                                                                                                                      <  4:380623/97‑1 (MQ=255)

                                               aTGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCCGCCGGACTTGGGTATtcc                                                                                          >  1:1045828/1‑141 (MQ=255)

                                               aTGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATtcc                                                                                          <  2:1030110/141‑1 (MQ=255)

                                                   gCCTGACAAGCTGCCGAGCTGCCCCATCCCGCGAGAATAAAAAAGAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCa                                                                                      <  4:196092/141‑1 (MQ=18)

                                                     ccGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTGCAAGACCGATCCCTTCAGCCGGACGGGGGTATTCCTCCagg                                                                                    >  3:229219/1‑139 (MQ=255)

                                                                ccGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATtatatggac                                                                         <  1:558100/141‑6 (MQ=14)

                                                                  gAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAAt                                                                       <  1:829734/141‑1 (MQ=255)

                                                                  gAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAAt                                                                       <  3:364108/141‑1 (MQ=255)

                                                                       gCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTc                                                                                                                                   >  1:605526/1‑76 (MQ=17)

                                                                       gCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTc                                                                                                                                   <  2:605526/76‑1 (MQ=17)

                                                                        cTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATATGTACCTCGcagg                                                                 >  2:647338/1‑138 (MQ=255)

                                                                        cTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTat                                                                 <  1:737741/141‑1 (MQ=255)

                                                                         tCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTgtt                                                                >  3:156484/1‑141 (MQ=255)

                                                                          ctgtCCGGCCATAATGCAAAATAATGGTGGAGGAAGAGGGAGTCGCCCCCGCGCGGCCCGTTGAGGCGCTGTCGGTTTTCGATGCCGATCCCTTCAACCGGACTTGCGTTTTCCTCCATTATTATAGGTACATCGCTatta                                                               <  4:216080/138‑1 (MQ=255)

                                                                          ccttCCCGCGATAAGAAAAAGTGTTGGCGGAGCGTGAGGGGTGGGAGGCCGTGGTGCCGGTGAATGGCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTatta                                                               <  1:99129/138‑1 (MQ=12)

                                                                          ccATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTatca                                                               <  2:763342/141‑3 (MQ=255)

                                                                           cATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTattaa                                                              <  3:56851/141‑1 (MQ=255)

                                                                            aTCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAACTCGCTgttaat                                                             >  4:432149/1‑141 (MQ=255)

                                                                            aTCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTattaat                                                             <  1:771383/141‑1 (MQ=255)

                                                                            aTCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTattaat                                                             >  1:152218/1‑141 (MQ=255)

                                                                                 gcgATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATaa                                                        >  1:568700/1‑141 (MQ=255)

                                                                                 gcgATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATaa                                                        >  1:568578/1‑141 (MQ=255)

                                                                                  cgATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATaaa                                                       >  1:258015/1‑141 (MQ=255)

                                                                                  cgATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATaaa                                                       <  2:72924/141‑1 (MQ=255)

                                                                                  cgATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATaaa                                                       <  4:420696/141‑1 (MQ=255)

                                                                                    ataataAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAAt                                                     <  2:960054/141‑1 (MQ=255)

                                                                                    ataataAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAAt                                                     <  1:720807/141‑1 (MQ=255)

                                                                                    ataataAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAAt                                                     <  2:856823/141‑1 (MQ=255)

                                                                                    ataataAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAAt                                                     <  2:139947/141‑1 (MQ=255)

                                                                                    ataataAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAAt                                                     <  2:638053/141‑1 (MQ=255)

                                                                                    ataataAAAAATAATGGCCGAGGAAGAGGGAGTCGAACCCCCGCGGCCCGTTAAGGCCGTGTCGGTTTTCAAGTCCGAGCCNTTCANCCGGACTNGGGTATTCCTCCAGTATTATAGGTAAATCGTCATGAATTATaaagt                                                     <  4:451084/141‑3 (MQ=255)

                                                                                        taaAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTaaa                                                 <  1:622072/141‑1 (MQ=255)

                                                                                          aaaaaTAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATg                                               >  3:270947/1‑141 (MQ=255)

                                                                                            aaaTAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGc                                             <  4:300254/141‑1 (MQ=255)

                                                                                             aataatGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAAGCGCTATTAATTATAAAATTAAATGGCg                                            <  3:104083/141‑1 (MQ=255)

                                                                                             aactaaggCGGTGGAAGAGCGATTCGAGCCACGGCGGCCCGGTAAGGCCCTGTCGGGTTGCAAGACCGATCCCATCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAGATTAAATGGCg                                            <  2:40795/135‑1 (MQ=21)

                                                                                              ataatGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCgg                                           <  1:892095/141‑1 (MQ=255)

                                                                                              ataatGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCgg                                           <  1:547303/141‑1 (MQ=255)

                                                                                              ataatGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGCTTTTTTTTACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTGTAGGTAAATCGCTATTAATTATAAAATTAAATGGCgg                                           <  1:782614/141‑1 (MQ=25)

                                                                                                 atGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGtct                                        >  3:100184/1‑141 (MQ=255)

                                                                                                  ggtcGGAGGAAGAGGGATTCGACCGCCCGCGGCCCGTTAAGGCCCTGGCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGtctc                                       <  1:274238/138‑1 (MQ=255)

                                                                                                       gaggaAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGTCTCGACgg                                  <  1:382673/141‑1 (MQ=255)

                                                                                                                 gATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAGTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGTCTCGACGGGAATCGAAcc                        <  4:410994/141‑1 (MQ=255)

                                                                                                                  aTTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTAATATAGGTAAATAGACCCTAAGAATAAAATTAAATGGCGGTCTCGACGGGAATCGAAccc                       <  1:520611/141‑1 (MQ=21)

                                                                                                                   ttCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGTCTCGTCGGGAATCGAACCcg                      >  1:899916/1‑141 (MQ=255)

                                                                                                                         cccccGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAACTGGCGGTCTCGACGGGAATCGACCCCGCGAtct                >  3:59193/1‑141 (MQ=255)

                                                                                                                         cccccGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAATATTATATGTAAATCGCTATTCTCGATCCAGCTACATGACGGTCTCCACGGGAATCGCACCCACGAtca                >  2:962950/1‑140 (MQ=17)

                                                                                                                           cccGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGTCTCGACGGGAATCGAACCCGCGATCTcc              <  2:611747/141‑1 (MQ=255)

                                                                                                                           cccGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGTCTCGACGGGAATCGAACCCGCGATCTcc              >  1:425512/1‑141 (MQ=255)

                                                                                                                                      ttcaGGCCCTGTCGCTTTTCCAGACCGGCCCCTTCAGCCGCACCAAGGTATTCCTCCACTATTATAGGTAAAGCGCTATTAATTATAAGATTAAATGGCGGTCTCGACGGGAATCGAACCCGCGATCTCCTGCGTGAcagg   <  1:1082373/138‑1 (MQ=25)

                                                                                                                                      ttAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATAATTATAGGTAAATCGCTATTATTTATACAATTAAATGGCGGTCTCGACGGGAATCGAACCCCCGATCTCCTGCGTGAccgg   >  2:79405/1‑141 (MQ=37)

                                                                                                                                       tAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGGAAATCGCTATTAATTATAAAATTAAATGGCGGTCTCGACGGGAATCGAACCCGCGAACTCCTGCGTGCcaggc  >  4:131564/1‑141 (MQ=255)

CTATAAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGTCTCGACGGGAATCGAACCCGCGATCTCCTGCGTGACAGGC  >  USA300TCH1516_ALE/1996883‑1997158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`