Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,880,657 C→T 5.8% R1521H (CGC→CAC) ‡ ebh_2 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,880,6570CT 5.8% 440.1 / 8.7 137R1521H (CGC→CAC) ‡ebh_2Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base C (66/63);  new base T (6/2);  total (72/65)
Fisher's exact test for biased strand distribution p-value = 2.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 148 total aligned reads displayed.

CATTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATA  >  USA300TCH1516_ALE/1880517‑1880797
                                                                                                                                            |                                                                                                                                            
cATTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTcgc                                                                                                                                              >  1:130580/1‑141 (MQ=255)
 aTTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTcgcg                                                                                                                                             <  2:111363/141‑1 (MQ=255)
  tttGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCgcgc                                                                                                                                            >  3:77994/1‑141 (MQ=255)
  tttGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCgcgc                                                                                                                                            >  1:308242/1‑141 (MQ=255)
  tttGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCgcgc                                                                                                                                            <  2:382856/141‑1 (MQ=255)
   ttGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGCTTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCt                                                                                                                                           >  1:1027333/1‑141 (MQ=255)
   ttGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCt                                                                                                                                           <  1:887249/141‑1 (MQ=255)
   ttGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCAGCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCt                                                                                                                                           <  1:135666/141‑1 (MQ=255)
    tGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTGGCTCAATTTCGCGCtt                                                                                                                                          >  3:298063/1‑141 (MQ=255)
      tttCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCtttt                                                                                                                                        >  1:627211/1‑141 (MQ=255)
          aaCACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGc                                                                                                                                    >  2:700881/1‑141 (MQ=255)
            cacGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCATTTTCGCGCTTTTTAGCtt                                                                                                                                  >  2:56047/1‑141 (MQ=255)
             acGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTc                                                                                                                                 <  1:841723/141‑1 (MQ=255)
              cGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCa                                                                                                                                <  2:337175/141‑1 (MQ=255)
                tttCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCata                                                                                                                              >  1:393618/1‑141 (MQ=255)
                   cacaTCATGATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTTTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttt                                                                                                                           >  4:11291/1‑140 (MQ=255)
                    acaTCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgt                                                                                                                          <  3:248864/141‑1 (MQ=255)
                    acaTCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgt                                                                                                                          <  2:903915/141‑1 (MQ=255)
                    acaTCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgt                                                                                                                          >  2:608253/1‑141 (MQ=255)
                    acaTCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgt                                                                                                                          <  2:234660/141‑1 (MQ=255)
                    acaTCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgt                                                                                                                          <  2:165126/141‑1 (MQ=255)
                      atcatTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgttg                                                                                                                        <  1:1043791/141‑1 (MQ=255)
                         attattCGCTGTGGGTTGGTCTATGTTTTGTAATGCACGTTTTTCATTATTCTCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCATTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgtgtctt                                                                                                                     >  2:222451/1‑136 (MQ=255)
                             ttCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgtt                                                                                                                         >  3:142168/1‑133 (MQ=255)
                             ttCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgtt                                                                                                                         <  4:142168/133‑1 (MQ=255)
                              tCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTg                                                                                                                <  2:826172/141‑1 (MQ=255)
                              tCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTg                                                                                                                <  1:986575/141‑1 (MQ=255)
                               cGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGc                                                                                                               >  2:610558/1‑141 (MQ=255)
                               cGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGc                                                                                                               >  4:124267/1‑141 (MQ=255)
                               cGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGc                                                                                                               >  1:1048444/1‑141 (MQ=255)
                                 cTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCAt                                                                                                             >  3:365137/1‑141 (MQ=255)
                                   aTTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCAtct                                                                                                           >  2:392132/1‑141 (MQ=255)
                                         tGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTagca                                                                                                     >  3:317975/1‑141 (MQ=255)
                                         tGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTagca                                                                                                     >  1:1014219/1‑141 (MQ=255)
                                           aTCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTagcagc                                                                                                   <  1:1002883/141‑1 (MQ=255)
                                              gATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAgctgc                                                                                                <  1:591143/141‑1 (MQ=255)
                                               aTGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAgctgct                                                                                               <  2:57089/141‑1 (MQ=255)
                                               aTGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAgctgct                                                                                               <  4:449292/141‑1 (MQ=255)
                                               aTGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAgctgct                                                                                               <  4:449295/141‑1 (MQ=255)
                                                tGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGctc                                                                                              >  1:137034/1‑141 (MQ=255)
                                                tGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGctc                                                                                              >  1:351674/1‑141 (MQ=255)
                                                 gTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGctct                                                                                             <  2:259944/141‑1 (MQ=255)
                                                     tGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACa                                                                                         <  2:671002/141‑1 (MQ=255)
                                                     tGTAATGCACGTATTTCATTACTCGCTAATTGACTTAAAGCAACTTGTTATTCTTCATCAGTCGCATCTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTAATATTATTACTCAAATGCATCTCTAGCAGATGATCTAACa                                                                                         >  2:288019/1‑141 (MQ=255)
                                                      gTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACaa                                                                                        <  2:63437/141‑1 (MQ=255)
                                                       tAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAat                                                                                       <  2:885875/141‑1 (MQ=255)
                                                        aaTGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAata                                                                                      >  3:174575/1‑141 (MQ=255)
                                                          tGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATg                                                                                    <  2:966987/141‑1 (MQ=255)
                                                           gCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATgg                                                                                   >  1:624967/1‑141 (MQ=255)
                                                             aCGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTc                                                                                 <  1:781186/141‑1 (MQ=255)
                                                             aCGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTc                                                                                 <  4:2737/141‑1 (MQ=255)
                                                                 ttttCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGc                                                                             <  1:581151/141‑1 (MQ=255)
                                                                 ttttCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGc                                                                             >  1:770762/1‑141 (MQ=255)
                                                                    tCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTc                                                                          >  3:250195/1‑141 (MQ=255)
                                                                      attattCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCgctac                                                                        >  4:302775/1‑137 (MQ=255)
                                                                      attattCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCgctac                                                                        >  1:266696/1‑137 (MQ=255)
                                                                      attattCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCgctac                                                                        >  4:417665/1‑137 (MQ=255)
                                                                        tattCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTa                                                                      >  1:599773/1‑141 (MQ=255)
                                                                         tttttttAATTGATTTAAAGCAACTTGTTTTTTTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCTCGCTTTTTAGCTTTATATTGTTTCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATTGTCTGGCGTCACTaa                                                                     >  4:307760/7‑141 (MQ=255)
                                                                            cGCTAATTGATTTAAAGCAACTTGTTTTTTTTTTTTTTTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCTTCTCTAGCTTCTGCTCTTTCAATATGGTCTGTCGTCACTAAAGc                                                                  >  2:638967/1‑141 (MQ=255)
                                                                            cGCTAATTGATTTAAAGCAACTTGTTTTTTTTCTTTTTTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCGCTACTAAAGc                                                                  >  2:553394/1‑141 (MQ=255)
                                                                            cGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGc                                                                  <  2:52810/141‑1 (MQ=255)
                                                                              cTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCaa                                                                <  3:169884/141‑1 (MQ=255)
                                                                               tAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAAt                                                               >  2:750399/1‑141 (MQ=255)
                                                                                aaTTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATg                                                              <  2:707457/141‑1 (MQ=255)
                                                                                          aaGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAgcg                                                    <  3:48522/141‑1 (MQ=255)
                                                                                               aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTTATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                               >  2:907367/1‑141 (MQ=255)
                                                                                               aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                               >  3:418040/1‑141 (MQ=255)
                                                                                               aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                               >  3:152818/1‑141 (MQ=255)
                                                                                               aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                               >  1:388911/1‑141 (MQ=255)
                                                                                               aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                               >  1:461685/1‑141 (MQ=255)
                                                                                               aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                               <  2:1027333/141‑1 (MQ=255)
                                                                                               aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                               >  4:397000/1‑141 (MQ=255)
                                                                                               aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                               >  4:363787/1‑141 (MQ=255)
                                                                                                  tGTTTTTCTTCATCAGGCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACtt                                            >  4:204606/1‑141 (MQ=255)
                                                                                                   gTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACttg                                           >  1:68614/1‑141 (MQ=255)
                                                                                                    tttttCTTCATCAGTCGCATGTTCCGCTTGCTCGATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACttgt                                          <  2:369565/141‑1 (MQ=255)
                                                                                                              tcaGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTGCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTAtcatca                                >  1:1062966/1‑141 (MQ=255)
                                                                                                                  tCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACtt                            >  1:531389/1‑141 (MQ=255)
                                                                                                                   cGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAAcaag                           <  1:755526/141‑4 (MQ=255)
                                                                                                                   cGCATGTTCCGCTTGCCCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCACCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCCAGCGCTTGACTTGTTGTACCCTCAACctg                           >  2:688455/1‑141 (MQ=255)
                                                                                                                    gCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgt                          <  2:132540/141‑1 (MQ=255)
                                                                                                                     cATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgtt                         <  3:399199/141‑1 (MQ=255)
                                                                                                                     cATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgtt                         <  4:362767/141‑1 (MQ=255)
                                                                                                                     cATGTTCCGCTCGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgtt                         <  3:365245/141‑1 (MQ=255)
                                                                                                                      aTGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgttg                        <  3:339573/141‑1 (MQ=255)
                                                                                                                       tGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACACTATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGa                       >  3:335211/1‑141 (MQ=255)
                                                                                                                        gTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGAt                      <  2:736455/141‑1 (MQ=255)
                                                                                                                           ccGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTg                   >  2:979980/1‑141 (MQ=255)
                                                                                                                              cTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTc                >  1:753207/1‑141 (MQ=255)
                                                                                                                                 gCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCtat             <  2:1075052/141‑1 (MQ=255)
                                                                                                                                  cTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCtatt            <  2:316563/141‑1 (MQ=255)
                                                                                                                                    cAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCtattat          >  3:26570/1‑141 (MQ=255)
                                                                                                                                    cAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCtattat          >  2:393019/1‑141 (MQ=255)
                                                                                                                                     aaTTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTattatt         <  2:46876/141‑1 (MQ=255)
                                                                                                                                      aTTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTc        <  4:26701/141‑1 (MQ=255)
                                                                                                                                      aTTTCGCGCTTTTTAGCTTCATATTGTGGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATTGTCATTCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTc        >  1:702942/1‑141 (MQ=255)
                                                                                                                                            cgcTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAata  >  1:649541/1‑141 (MQ=255)
                                                                                                                                            cgcTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAata  >  1:649408/1‑141 (MQ=255)
                                                                                                                                            cgcTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGCCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAata  >  1:70011/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CATTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATA  >  USA300TCH1516_ALE/1880517‑1880797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: