BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,792,748	A→T	8.3%	S367T (TCG→ACG)	polA_2 ←	DNA polymerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,792,748	0	A	T	8.3%	319.3 / 4.0	111	S367T (TCG→ACG)	polA_2	DNA polymerase I
Reads supporting (aligned to +/- strand): ref base A (49/50); new base T (10/2); total (59/52)
Fisher's exact test for biased strand distribution p-value = 3.29e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

ATTGAACATCACTAATCGTACGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCAAATAGTTACCACCGTCTAATTCG  >  USA300TCH1516_ALE/1792610‑1792884

aTTGAACATCACTAATCGTACGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAAt                                                                                                                                        >  3:44617/1‑141 (MQ=255)

     aCATCACTAATCGTACGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGa                                                                                                                                   >  1:775914/1‑141 (MQ=255)

       aTCACTAATCGTACGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGAtt                                                                                                                                 <  4:371569/141‑1 (MQ=255)

       aTCACTAATCGTACGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGAtt                                                                                                                                 >  4:137565/1‑141 (MQ=255)

        tCACTAATCGTACGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGAttt                                                                                                                                <  1:195129/141‑1 (MQ=255)

            tAATCGTACGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTa                                                                                                                            <  1:489704/141‑1 (MQ=255)

            tAATCGTACGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTa                                                                                                                            <  2:910515/141‑1 (MQ=255)

             aaTCGTACGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTaa                                                                                                                           >  2:124409/1‑141 (MQ=255)

             aaTCGTACGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTaa                                                                                                                           >  1:706793/1‑141 (MQ=255)

             aaTCGTACGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTaa                                                                                                                           >  2:592163/1‑141 (MQ=255)

             aaTCGTACGAGATGGATCAATTATACAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATTCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGTATTCGGATTTTCTaa                                                                                                                           <  4:42797/141‑1 (MQ=255)

               tCGTACGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAAcc                                                                                                                         <  2:497552/141‑1 (MQ=255)

               tCGTACGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAACATCATATACGACTTTTTTCGAATTCGGATTTTCTAAcc                                                                                                                         <  2:247934/141‑1 (MQ=255)

                cGTACGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCa                                                                                                                        >  2:965611/1‑141 (MQ=255)

                  tACGAGATGGATCAATTATATAACTTGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATg                                                                                                                      <  2:1022608/141‑1 (MQ=255)

                  tACGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATTTCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGTATTCGGATTTTCTAACCATg                                                                                                                      <  1:403584/141‑1 (MQ=255)

                   aCGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGa                                                                                                                     <  1:1069436/141‑1 (MQ=255)

                   aCGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGa                                                                                                                     >  1:829542/1‑141 (MQ=255)

                    cGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGaa                                                                                                                    >  4:209206/1‑141 (MQ=255)

                      agaTGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAAc                                                                                                                  <  1:526200/141‑1 (MQ=255)

                        aTGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTGGAATATCAATCCCCAATCTATGTGATGCTACATAGGTTTTTTTAGCATCATATACGACTTTCTTCGAATTCGGATTTTCTATCCATGAAAcgg                                                                                                                <  4:142214/141‑3 (MQ=255)

                          ggATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGt                                                                                                              <  4:30436/141‑1 (MQ=255)

                          ggATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGt                                                                                                              >  4:332330/1‑141 (MQ=255)

                          ggATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGt                                                                                                              <  4:56289/141‑1 (MQ=255)

                            aTCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTc                                                                                                            <  2:922394/141‑1 (MQ=255)

                            aTCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTc                                                                                                            >  2:307784/1‑141 (MQ=255)

                               aaTTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGAc                                                                                                         >  2:737174/1‑141 (MQ=255)

                               aaTTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGAc                                                                                                         <  3:394924/141‑1 (MQ=255)

                                  tatataACTGACCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATa                                                                                                      <  1:7133/141‑1 (MQ=255)

                                     ataACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCACTCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAatt                                                                                                   <  1:1028274/141‑1 (MQ=255)

                                     ataACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATaaat                                                                                                   <  1:628095/141‑3 (MQ=255)

                                          tGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAAttattta                                                                                              <  1:887116/141‑1 (MQ=255)

                                            gCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCTTTTTTGTATTTTCTAACCATGAAACTAGTTCGACATAATTATTTCTg                                                                                            >  4:219677/1‑141 (MQ=255)

                                            gCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATg                                                                                            >  2:876383/1‑141 (MQ=255)

                                                   taataTCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCg                                                                                     >  2:183255/1‑141 (MQ=255)

                                                   taataTCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCg                                                                                     >  3:148076/1‑141 (MQ=255)

                                                      tatCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCat                                                                                  >  3:75015/1‑141 (MQ=255)

                                                      tatCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCat                                                                                  >  1:44364/1‑141 (MQ=255)

                                                        tCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGCCATAATTATTTATGTCATCCGCatta                                                                                >  1:641824/1‑141 (MQ=255)

                                                        tCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCatta                                                                                <  2:52406/141‑1 (MQ=255)

                                                         cGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGTATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCattaa                                                                               >  2:613448/1‑141 (MQ=255)

                                                         cGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCattaa                                                                               >  1:866621/1‑141 (MQ=255)

                                                             agaaaTATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTATCATCATATACGACTTTTTTCTTTTTCTGATTTTCTTACCATGAAACTCGTTCGACATAATTATTTATCTCATCCGCattatttaa                                                                           >  4:51648/1‑140 (MQ=255)

                                                                aaTATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCTGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAat                                                                        >  2:1055627/1‑141 (MQ=255)

                                                                 atatTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGTATTTTCTAACTATGAAACTAGTTCGACATAATTATTTATGTCATCCTCATTAATTACAata                                                                       >  4:304192/1‑141 (MQ=255)

                                                                          atatCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCa                                                              >  3:457529/1‑141 (MQ=255)

                                                                          atatCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCa                                                              <  4:356922/141‑1 (MQ=255)

                                                                            atCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCAcc                                                            >  3:149130/1‑141 (MQ=255)

                                                                             tCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCt                                                           >  2:645840/1‑141 (MQ=255)

                                                                             tCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCt                                                           >  1:943872/1‑141 (MQ=255)

                                                                             tCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCt                                                           <  1:506180/141‑1 (MQ=255)

                                                                              cAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTg                                                          <  1:193571/141‑1 (MQ=255)

                                                                                   cccAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGtaaat                                                     >  3:407642/1‑141 (MQ=255)

                                                                                      aaTCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGtaaataaa                                                  <  1:863427/141‑1 (MQ=255)

                                                                                       aTCTATGTGATGCTACATATGTTGTTTTATCATCTTGTACTTCTTTTTTCTTGTTCGGATTTTCTAACCATGAAACTAGTTTGACATAATTATTTATGTCATCTGCATTTATTGCATTATGTTTCTCTCCTGttaataatt                                                 >  4:224403/1‑139 (MQ=255)

                                                                                        tCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTTTTATTTTTTTTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAaga                                                >  4:440234/1‑141 (MQ=255)

                                                                                        tCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAaga                                                <  1:437538/141‑1 (MQ=255)

                                                                                         cTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAAATCGGATTTTCTAACCATTAACCTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAACCAAagag                                               >  2:469557/1‑141 (MQ=255)

                                                                                          tATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAgaga                                              <  1:1015081/141‑1 (MQ=255)

                                                                                            tgtgATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAAt                                            >  2:704318/1‑141 (MQ=255)

                                                                                            tgtgATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAAt                                            <  4:147038/141‑1 (MQ=255)

                                                                                            tgtgATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAAt                                            <  4:147024/141‑1 (MQ=255)

                                                                                            tgtgATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAAt                                            >  2:90106/1‑141 (MQ=255)

                                                                                            tgtgATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAAt                                            >  1:89912/1‑141 (MQ=255)

                                                                                             gtgATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTGCAATATGTTTCTCACCTGTAAATAAAGAGAAtt                                           >  2:401691/1‑141 (MQ=255)

                                                                                               gATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAAtttt                                         <  2:166457/141‑1 (MQ=255)

                                                                                                   cTACATGTGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTaaaa                                     <  1:458701/141‑1 (MQ=255)

                                                                                                   cTACATATGTTTTTTTAGCATCATATACGACTTTTTTTTTTTTTTTTTTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAAttttttaa                                     >  2:422283/1‑137 (MQ=255)

                                                                                                     aCATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAata                                   >  4:442028/1‑141 (MQ=255)

                                                                                                          tGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCCTGCAACTAGTTCGAAATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATattatt                              <  1:648101/141‑1 (MQ=255)

                                                                                                           gTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTAttt                             <  4:397835/141‑1 (MQ=255)

                                                                                                            tttttttAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTc                            >  1:245487/1‑141 (MQ=255)

                                                                                                            tttttttAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTc                            >  1:245453/1‑141 (MQ=255)

                                                                                                             ttttttATCATCATATACGACTTTTTTCGTATTCGGATTTTTTAACCATGAAACTAGTTCGACATTATTATTTATGTCATCCGCATTAATTACTATATTTTTTTTACCTGTTAATTAAGTTAATTTTAAAATATTAttttt                           >  2:654434/1‑139 (MQ=255)

                                                                                                             ttttttAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCg                           <  2:992407/141‑1 (MQ=255)

                                                                                                              tttttAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTTACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGc                          >  2:930822/1‑141 (MQ=255)

                                                                                                              tttttAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGc                          <  4:436107/141‑1 (MQ=255)

                                                                                                              tttttAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGc                          <  2:489443/141‑1 (MQ=255)

                                                                                                              tttttAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGc                          <  3:172714/141‑1 (MQ=255)

                                                                                                              tatgtAGCATCATATACGACTTTTTTTTTTTTTTTTTTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTctc                          >  2:231872/5‑139 (MQ=255)

                                                                                                               ttttAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCa                         >  3:264695/1‑141 (MQ=255)

                                                                                                                 ttAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCaaa                       <  4:60751/141‑1 (MQ=255)

                                                                                                                  tAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCAAAt                      <  3:313391/141‑1 (MQ=255)

                                                                                                                  tAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCAAAt                      >  1:136428/1‑141 (MQ=255)

                                                                                                                  tAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCAAAt                      >  1:942549/1‑141 (MQ=255)

                                                                                                                   aGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCAAATa                     >  1:890845/1‑141 (MQ=255)

                                                                                                                    tcatcatATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCAAATAg                    >  2:307544/2‑141 (MQ=255)

                                                                                                                    gCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCAAATAg                    >  1:179888/1‑141 (MQ=255)

                                                                                                                         atatACGACTTTTTTCGTTTTTGTTTTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCTCAAATAGTTacc               >  4:256127/1‑141 (MQ=255)

                                                                                                                         atatACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCAAATAGTTacc               <  1:455195/141‑1 (MQ=255)

                                                                                                                         atatACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCAAATAGTTacc               >  1:496339/1‑141 (MQ=255)

                                                                                                                          tataCGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCAAATAGTTacca              <  3:263514/141‑1 (MQ=255)

                                                                                                                          tataCGACTTTTTTAGAATTCGGATTTTCTAACCATGAAACTATTGCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCAAATAGTTacca              <  1:77623/141‑1 (MQ=255)

                                                                                                                            taCGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCAAATAGTTaccacc            <  1:635131/141‑1 (MQ=255)

                                                                                                                               gACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCAAATAGTTACCACCGTc         >  4:61977/1‑141 (MQ=255)

                                                                                                                                 cTTTTTTCGAATTCGGATTTTCTCACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTTGATAAAGAGAATTTTAAAATATTATATCGCAAATAGTTACAACCGTCTa       <  2:923579/141‑1 (MQ=255)

                                                                                                                                 cTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCAAATAGTTACCACCGTCTa       <  1:930864/141‑1 (MQ=255)

                                                                                                                                   tttttCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCAAATAGTTACCACCGTCTAAt     <  2:462929/141‑1 (MQ=255)

                                                                                                                                     tcacGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTGATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCAAATAGTTACCACCGTCTAATTc   <  1:714095/138‑1 (MQ=255)

                                                                                                                                      ttCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCAAATAGTTACCACCGTCTAATTCg  >  1:1000011/1‑141 (MQ=255)

ATTGAACATCACTAATCGTACGAGATGGATCAATTATATAACTGGCCAACATAATATCGAAAGAAATATTTTGAATATCAATCCCCAATCTATGTGATGCTACATATGTTTTTTTAGCATCATATACGACTTTTTTCGAATTCGGATTTTCTAACCATGAAACTAGTTCGACATAATTATTTATGTCATCCGCATTAATTACAATATGTTTCTCACCTGTAAATAAAGAGAATTTTAAAATATTATTTCGCAAATAGTTACCACCGTCTAATTCG  >  USA300TCH1516_ALE/1792610‑1792884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`