Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,781,166 A→G 5.3% intergenic (‑138/+58) infC ← / ← lysP_2 Translation initiation factor IF‑3/Lysine‑specific permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,781,1660AG 5.3% 395.1 / 9.7 133intergenic (‑138/+58)infC/lysP_2Translation initiation factor IF‑3/Lysine‑specific permease
Reads supporting (aligned to +/- strand):  ref base A (71/54);  new base G (5/2);  total (77/56)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01

Only 100 of 140 total aligned reads displayed.

AAAAGGAAGAGCAGGTATAAAATACCCGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTCGTTTTATATCGAAGCT  >  USA300TCH1516_ALE/1781028‑1781306
                                                                                                                                          |                                                                                                                                            
aaaaGGAAGAGCAGGTATAAAATACCCGCTCTTCCTTATACACAGTTATCTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGttatt                                                                                                                                            >  2:720104/1‑141 (MQ=255)
   aGGAAGAGCAGGTATAAAATACCCGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATCCCACGTTATTCAt                                                                                                                                         >  4:250971/1‑141 (MQ=255)
    ggAAGAGCGGGTATTTTATACCTGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATc                                                                                                                                        >  2:758041/1‑141 (MQ=255)
    ggAAGAGCAGGTATAAAATACCCGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGAGTCGCGACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATc                                                                                                                                        >  1:564766/1‑141 (MQ=255)
     gAAGAGCAGGTATAAAATACCCGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCa                                                                                                                                       >  1:452658/1‑141 (MQ=255)
      aaGAGCAGGTATAAAATACCCGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCat                                                                                                                                      >  2:1023866/1‑141 (MQ=255)
       agagCAGGTATAAAATACCCGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCata                                                                                                                                     <  2:480471/141‑1 (MQ=255)
        gagCAGGGCTAAAATACCCGCTCTTCCTTATACACAGGTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTTAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTCTTAataataa                                                                                                                                    >  4:431114/1‑140 (MQ=255)
          gCAGGTATAAAATACCCGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATca                                                                                                                                  <  2:2434/141‑1 (MQ=255)
             ggTATAAAATACCCGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATcaaca                                                                                                                               <  1:339130/141‑1 (MQ=255)
              gTATAAAATACCCGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATcaacaa                                                                                                                              <  2:495390/141‑1 (MQ=255)
               tattttaTACCTGCTCTTCCTTATACACAGTTATGTGTAAGGTAATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAAt                                                                                                                             >  1:792219/7‑141 (MQ=255)
                ataAAATACCCGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAAtt                                                                                                                            <  2:51772/141‑1 (MQ=255)
                 ttttaTACCTGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTc                                                                                                                           >  2:1082867/5‑141 (MQ=255)
                 taAAATACCCGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTc                                                                                                                           >  4:384098/1‑141 (MQ=255)
                   aaaTACCCGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTcac                                                                                                                         <  1:681432/141‑1 (MQ=255)
                    aaTACCCGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTGCGTATTCAACGTTATTAATCATATCAACAATTcaca                                                                                                                        <  4:242449/141‑1 (MQ=255)
                       aCCCGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACAttt                                                                                                                     >  4:341475/1‑141 (MQ=255)
                       aCCCGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACAttt                                                                                                                     >  1:845323/1‑141 (MQ=255)
                        cccGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTa                                                                                                                    >  2:394373/1‑141 (MQ=255)
                            ctctTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTc                                                                                                                >  3:445249/1‑141 (MQ=255)
                            ctctTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTc                                                                                                                >  3:94534/1‑141 (MQ=255)
                                tCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAaca                                                                                                            <  1:438917/141‑1 (MQ=255)
                                  cTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACt                                                                                                          >  2:315909/1‑141 (MQ=255)
                                   ttATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACta                                                                                                         <  3:380974/141‑1 (MQ=255)
                                     ataCACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACtata                                                                                                       >  3:394846/1‑141 (MQ=255)
                                      taCACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATaa                                                                                                      <  2:46152/141‑1 (MQ=255)
                                          caGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGt                                                                                                  >  1:426012/1‑141 (MQ=255)
                                           aGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTa                                                                                                 <  1:52600/141‑1 (MQ=255)
                                            taactgtgTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTaa                                                                                                >  1:794629/5‑141 (MQ=255)
                                             ttATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACCGGTGAGCAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTGTTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAat                                                                                               <  1:213205/141‑1 (MQ=255)
                                                tgtgTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTCCTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAattat                                                                                            >  1:480456/1‑141 (MQ=255)
                                                         tgATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATtatttttatttt                                                                                   >  1:85936/1‑141 (MQ=255)
                                                           aTTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTCTTTTTATTTTaa                                                                                 >  1:125179/1‑141 (MQ=255)
                                                           aTTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTaa                                                                                 >  3:407396/1‑141 (MQ=255)
                                                           aTTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTaa                                                                                 <  2:413239/141‑1 (MQ=255)
                                                           aTTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTaa                                                                                 >  4:122159/1‑141 (MQ=255)
                                                            ttAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGAGCTTATAATTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAAc                                                                                >  4:198124/1‑141 (MQ=255)
                                                             tAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGGGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAAc                                                                                                     <  3:302529/119‑1 (MQ=255)
                                                              aaCCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCt                                                                              <  2:1028768/141‑1 (MQ=255)
                                                               aCCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCtt                                                                             >  4:23640/1‑141 (MQ=255)
                                                                   gCCACCTGCTTTATGTGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACACTTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTAt                                                                         <  1:550674/141‑1 (MQ=255)
                                                                   gCCAACTGCTTTATGTGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACACTTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTAt                                                                         <  1:550733/141‑1 (MQ=255)
                                                                    ccAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTAtt                                                                        <  1:1038824/141‑1 (MQ=255)
                                                                    ccAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTAtt                                                                        <  1:1038761/141‑1 (MQ=255)
                                                                    ccAACTGCTTTACGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAATACTATAACTGTAATTATTTTTATTTTAACCTTTTAtt                                                                        <  1:784200/141‑1 (MQ=255)
                                                                     cAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTAttt                                                                       >  2:684761/1‑141 (MQ=255)
                                                                      aaCTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTGTTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTc                                                                      <  3:18919/141‑1 (MQ=255)
                                                                      aaCTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTc                                                                      >  1:843205/1‑141 (MQ=255)
                                                                         tGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATCACTGTAATTATTTTTATTTTAACCTTTTATTTCATc                                                                   <  2:956077/141‑1 (MQ=255)
                                                                            tttATGCGTCGCTACAGGTGAGAAGCGGGTTCTTCTGTTGGTTTCTTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTTAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTACATCCAt                                                                <  3:359609/141‑1 (MQ=255)
                                                                            tttATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCAt                                                                >  4:50335/1‑141 (MQ=255)
                                                                                   gTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACg                                                         <  2:447177/141‑1 (MQ=255)
                                                                                      gCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCt                                                      <  1:1023866/141‑1 (MQ=255)
                                                                                          cAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGAc                                                  >  2:253759/1‑141 (MQ=255)
                                                                                               gagaAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTaaa                                             >  2:72246/1‑141 (MQ=255)
                                                                                                agaAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAAt                                            <  3:122159/141‑1 (MQ=255)
                                                                                                 gaAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTGTTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATc                                           >  2:133608/1‑141 (MQ=255)
                                                                                                 gaAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATc                                           <  2:1057476/141‑1 (MQ=255)
                                                                                                    gcacccgCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTAc                                        >  1:1001444/7‑141 (MQ=255)
                                                                                                    gcacccgCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTAc                                        >  1:1001411/7‑141 (MQ=255)
                                                                                                    gCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTGTTAATCATATCAACAATTCACATTTAAGTCAACACTATAGCTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTAc                                        >  2:574716/1‑141 (MQ=255)
                                                                                                     cGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACt                                       <  4:394846/141‑1 (MQ=255)
                                                                                                     cGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACt                                       <  2:989420/141‑1 (MQ=255)
                                                                                                     cGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGGAAATCTACt                                       <  1:956619/141‑1 (MQ=255)
                                                                                                      gggTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACtt                                      >  1:936856/1‑141 (MQ=255)
                                                                                                      gggTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACtt                                      >  3:311996/1‑141 (MQ=255)
                                                                                                      gggTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACtt                                      >  3:299676/1‑141 (MQ=255)
                                                                                                        gTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGt                                    >  1:325745/1‑141 (MQ=255)
                                                                                                        gTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGt                                    <  2:478520/141‑1 (MQ=255)
                                                                                                        gTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGt                                    <  2:709043/141‑1 (MQ=255)
                                                                                                         tGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATGTCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGtt                                   >  3:266340/1‑141 (MQ=255)
                                                                                                          gCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTCGTTc                                  >  1:771052/1‑141 (MQ=255)
                                                                                                           cTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCt                                 >  2:79304/1‑141 (MQ=255)
                                                                                                           cTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCt                                 >  3:329959/1‑141 (MQ=255)
                                                                                                           cTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCt                                 >  2:71930/1‑141 (MQ=255)
                                                                                                               tACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATg                             <  3:236538/141‑1 (MQ=255)
                                                                                                                aCTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAATAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTTACACGTCTTGAAGTAAGTCTACTTTTTCTAATgg                            >  4:128953/1‑141 (MQ=255)
                                                                                                                    ggTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAAtt                        <  1:210927/141‑1 (MQ=255)
                                                                                                                     gTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAAttt                       >  2:297426/1‑141 (MQ=255)
                                                                                                                     gTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAAttt                       <  1:867262/141‑1 (MQ=255)
                                                                                                                         gtttcgtATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAAttttttt                   >  4:321971/1‑141 (MQ=255)
                                                                                                                         gtttcgtATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAAttttttt                   >  3:187254/1‑141 (MQ=255)
                                                                                                                          tttcgtATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTc                  <  3:431114/141‑1 (MQ=255)
                                                                                                                             cgtATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTCGtt               >  2:947361/1‑141 (MQ=255)
                                                                                                                              gtATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTCGttt              >  4:252203/1‑141 (MQ=255)
                                                                                                                              gtATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTCGttt              <  1:394373/141‑1 (MQ=255)
                                                                                                                                aTTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTCGTTTta            <  1:449002/141‑1 (MQ=255)
                                                                                                                                   cAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTCGTTTTatat         >  4:402778/1‑141 (MQ=255)
                                                                                                                                   cAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTCGTTTTatat         >  2:924333/1‑141 (MQ=255)
                                                                                                                                    aaCGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTCGTTTTATATc        >  1:516115/1‑141 (MQ=255)
                                                                                                                                     aCGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTCGTTTTATATCg       >  1:777570/1‑141 (MQ=255)
                                                                                                                                     aCGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTCGTTTTATATCg       >  1:469959/1‑141 (MQ=255)
                                                                                                                                      cGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTCGTTTTATATCGa      <  1:816586/141‑1 (MQ=255)
                                                                                                                                       gTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTCGTTTTATATCGaa     <  1:478001/141‑1 (MQ=255)
                                                                                                                                       gTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTCGTTTTATATCGaa     <  3:27616/141‑1 (MQ=255)
                                                                                                                                        ttattaATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTCGTTTTATATCGAAg    >  1:887988/1‑141 (MQ=255)
                                                                                                                                        ttattaATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTCGTTTTATATCGAAg    >  2:362568/1‑141 (MQ=255)
                                                                                                                                          attaATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACTCGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTCGTTTTATATCGAAGCt  <  2:465314/141‑1 (MQ=255)
                                                                                                                                          attaATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTCGTTTTATATCGAAGCt  >  1:822457/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                            
AAAAGGAAGAGCAGGTATAAAATACCCGCTCTTCCTTATACACAGTTATGTGTAATGTGATTAACCTGCCAACTGCTTTATGCGTCGCTACAGGTGAGAAGCGGGTGCTTCTACTTGGTTCGTTTCGTATTCAACGTTATTAATCATATCAACAATTCACATTTAAGTCAACACTATAACTGTAATTATTTTTATTTTAACCTTTTATTTCATCCATTGACACGTCTTGACGTAAATCTACTTGTTCTAATGGAATTTTTTTCGTTTTATATCGAAGCT  >  USA300TCH1516_ALE/1781028‑1781306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: