| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,712,214 | G→T | 5.9% | Q150K (CAA→AAA) | mntH_2 ← | Divalent metal cation transporter MntH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,712,214 | 0 | G | T | 5.9% | 412.0 / 2.3 | 130 | Q150K (CAA→AAA) | mntH_2 | Divalent metal cation transporter MntH |
| Reads supporting (aligned to +/- strand): ref base G (53/67); new base T (8/2); total (61/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 134 total aligned reads displayed.
GACTAATTTCATTGGATGTTCTGGCGCAAATGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCAATATTAAAAGCTAGACCACCAAAT > USA300TCH1516_ALE/1712074‑1712353 | aCTAATTTCATTGGATGTTCTGGCGCAAATGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTgg < 3:44733/141‑1 (MQ=255) aCTAATTTCATTGGATGTTCTGGCGCAAATGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTgg < 1:1000529/141‑1 (MQ=255) aTTTCATTGGATGTTCTGGCGCAAATGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCAc > 1:427978/1‑141 (MQ=255) tttCATTGGATGTTCTGGCGCAAATGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACt < 3:131733/141‑1 (MQ=255) tttCATTGGATGTTCTGGCGCAAATGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACt > 3:126630/1‑141 (MQ=255) tCATTGGATGTTCTGGCGCAAATGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACttt < 2:665154/141‑1 (MQ=255) cATTGGATGTTCTGGCGCAAATGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACtttt < 2:411194/141‑1 (MQ=255) cATTGGATGTTCTGGCGCAAATGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACtttt < 2:421449/141‑1 (MQ=255) tGGATGTTCTGGCGCAAATGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTttact > 1:392346/1‑141 (MQ=255) aTGTTCTGGCGCAAATGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACAACACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTttacttac > 2:186613/1‑141 (MQ=255) tCTGGCGCAAATGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAATATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAg < 1:477452/141‑1 (MQ=255) cTGGCGCAAATGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGa < 2:312741/141‑1 (MQ=255) cTGGCGCAAATGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGa > 1:749037/1‑141 (MQ=255) ggCGCAAATGTATGCACAAAAGCATCACCATAAGGTGGGTTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGAtt > 1:414429/1‑141 (MQ=255) aaaTGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATTACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAatat > 2:129933/1‑141 (MQ=255) aaaTGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAatat < 1:469462/141‑1 (MQ=255) aaaTGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAatat < 2:389039/141‑1 (MQ=255) aaaTGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAatat > 2:296307/1‑141 (MQ=255) aaTGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATAtt < 1:545588/141‑1 (MQ=255) tGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGc < 2:875085/141‑1 (MQ=255) tGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGc < 2:976390/141‑1 (MQ=255) gTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCa < 2:137681/141‑1 (MQ=255) tATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCaa > 4:131148/1‑141 (MQ=255) tGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAg > 2:744137/1‑141 (MQ=255) cacaAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGAt < 1:639834/141‑1 (MQ=255) cacaAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCAACACAATACCAAGAATCATTGAAACAACATCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGAt < 4:306476/141‑1 (MQ=255) caAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCCCAATACCAAGAATCATTGCAACAACGTCAATACTTTAATGGCAATCTTTACTTCCCCAGATTACTACTGCACAGATTg > 1:322355/1‑141 (MQ=255) caAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTTTCCACTTTTACTTACAAAGATTAATATTGCAAAGATTg > 2:225270/1‑141 (MQ=255) cATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTaa > 2:505902/1‑141 (MQ=255) aTCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAAt < 1:1055781/141‑1 (MQ=255) tCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTCCAAAGATTAATATTGCAAAGATTGCAGTAATa > 3:108718/1‑141 (MQ=255) cACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAg < 3:176233/141‑1 (MQ=255) aCCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGc > 3:247323/1‑141 (MQ=255) cATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTg < 1:118189/141‑1 (MQ=255) aTAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTgc < 4:212721/141‑1 (MQ=255) ggTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGccc < 2:518615/141‑1 (MQ=255) tGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTCTTTGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCa < 2:593227/141‑1 (MQ=255) ggATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTTTCCTTTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCAt > 4:319734/1‑141 (MQ=255) ggATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTTTCCTCTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCAt > 2:112456/1‑141 (MQ=255) ggATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCAt < 3:424633/141‑1 (MQ=255) ggATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCAt > 3:254566/1‑141 (MQ=255) aTTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCAttt < 4:257794/141‑1 (MQ=255) ttAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCAtttt < 4:33177/141‑1 (MQ=255) ttAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCAtttt > 1:204517/1‑141 (MQ=255) aGAAACAAACATCACATATGCCACAACTAAACTCATCACACTACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGAGTGCAGGAATAGCTGCGCCCCATTTTAc < 2:332964/141‑1 (MQ=255) acaaacaTCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCt > 2:906275/1‑141 (MQ=255) aaacaTCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTaa > 1:556360/1‑141 (MQ=255) aaacaTCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTaa > 2:10133/1‑141 (MQ=255) caTCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATcc < 1:1022767/141‑1 (MQ=255) caTCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTATTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATcc < 2:76517/141‑1 (MQ=255) aTCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCa > 1:170371/1‑141 (MQ=255) tCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCaa > 3:11864/1‑141 (MQ=255) cacaTATGCACCAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCaaa < 4:200148/141‑1 (MQ=255) gcaTATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAGTTTTTTGGCCACTTTTTCTTTCAATTTTTAATATTGCAAAGAGTGGAGTAATAGCTGTGTCCCATTTTACATCTAATCCaaaa > 1:542297/2‑141 (MQ=255) acaTATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCaaaa > 3:296839/1‑141 (MQ=255) acaTATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCaaaa > 3:279270/1‑141 (MQ=255) atatGCCACAACTAAAATCATCACAATAGGAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGAGTGCAGTAATATCTGCGTCCCATTTCACAACTAATCCaataat > 2:908238/1‑141 (MQ=255) atatGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAAt < 2:724455/141‑1 (MQ=255) atGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTg < 3:389957/141‑1 (MQ=255) atGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTg < 3:400250/141‑1 (MQ=255) ccACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGTCTACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCAt > 2:984093/1‑141 (MQ=255) cacaACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTTTTTTTTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCTACAAATTGCAtt > 4:368218/1‑141 (MQ=255) aCTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTtaaa < 1:47695/141‑1 (MQ=255) cTAAAATCATCACAATACCATGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTATTCCAAAAATTGCATTtaaac < 4:108992/141‑1 (MQ=255) tAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTtaaacc < 2:525373/141‑1 (MQ=255) aaaaTCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTtaaacct > 2:980545/1‑141 (MQ=255) aaaTCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTtaaaccta < 4:4858/141‑1 (MQ=255) aaTCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTtaaacctaa > 2:726310/1‑141 (MQ=255) atcatcACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTtaaacctaaa < 1:324120/141‑1 (MQ=255) tcatcaCAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTtaaacctaaac < 2:113301/141‑1 (MQ=255) tcatcaCAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTtaaacctaaac < 1:534408/141‑1 (MQ=255) atcaCAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTTTTTTTTTTTTTTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCATAAATTTCATTtaaacctaaacca > 4:70730/1‑140 (MQ=255) atcaCAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTTTTTATTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCg > 2:952369/1‑141 (MQ=255) aaTACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCAcc < 3:295610/141‑1 (MQ=255) aCCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGc < 1:803197/141‑1 (MQ=255) cAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCaa < 2:727481/141‑1 (MQ=255) cAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCaa > 3:293784/1‑141 (MQ=255) aGAATCATTGAAACAACGTCCATAATTTTTTTTTTTTTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATTTAATCCATATATTAAATTTAAACATAAACCGGCAACAGCAata > 2:987610/1‑141 (MQ=255) aGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAata < 3:375694/141‑1 (MQ=255) gAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAatat > 2:15489/1‑141 (MQ=255) aaTCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATAtt > 4:406024/1‑141 (MQ=255) cATTAAAACAACGTCCATAATTTTTTGGCCACTATTACTTACAAAGATTAATATTACAAAAATTGCAGTAATAGCTGCGCCCCATTTTACAACTAAACCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTAcc < 2:1032200/141‑1 (MQ=255) ttGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCaa > 1:830058/1‑141 (MQ=255) ttGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCaa < 2:156744/141‑1 (MQ=255) aaaCAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCAatat < 1:587920/141‑1 (MQ=255) aaaCAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCAatat < 2:716269/141‑1 (MQ=255) aaaCAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCAatat < 2:1001613/141‑1 (MQ=255) aacGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCAATATTaaa < 3:406598/141‑1 (MQ=255) acGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCAATATTaaaa > 3:173727/1‑141 (MQ=255) acGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCAATATTaaaa > 2:194795/1‑141 (MQ=255) gTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCAATATTAAAAGc > 3:230942/1‑141 (MQ=255) gTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCAATATTAAAAGc < 1:186613/141‑1 (MQ=255) ccATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTCAACCGCCATCAGCAATATTACCAATATTAAAAGCTa > 1:883344/1‑141 (MQ=255) ccATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCAATATTAAAAGCTa > 1:520558/1‑141 (MQ=255) cATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCAATATTAAAAGCTAg < 1:51231/141‑1 (MQ=255) aTAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCAATATTAAAAGCTAGa < 4:254566/141‑1 (MQ=255) aTAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCAATATTAAAAGCTAGa > 3:28160/1‑141 (MQ=255) aTAATTTTTTGGCCACTTTTACTTACAAAAATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTAAAAAAAAAAAAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCAATATTAAAAGCTAGa < 4:275066/141‑1 (MQ=255) aTAATTCTTTGGCCACTTTTACTTACGAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCAATATTAAAAGCTAGa < 2:859115/141‑1 (MQ=255) tGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCAATATTAAAAGCTAGACCACCAAAt > 3:26169/1‑141 (MQ=255) | GACTAATTTCATTGGATGTTCTGGCGCAAATGTATGCACAAAAGCATCACCATAAGGTGGATTAGAAACAAACATCACATATGCCACAACTAAAATCATCACAATACCAAGAATCATTGAAACAACGTCCATAATTTTTTGGCCACTTTTACTTACAAAGATTAATATTGCAAAGATTGCAGTAATAGCTGCGCCCCATTTTACATCTAATCCAAAAATTGCATTTAAACCTAAACCGGCACCAGCAATATTACCAATATTAAAAGCTAGACCACCAAAT > USA300TCH1516_ALE/1712074‑1712353 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |