| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,622,149 | C→T | 5.8% | V405V (GTC→GTT) | zwf → | Glucose‑6‑phosphate 1‑dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,622,149 | 0 | C | T | 5.8% | 360.0 / 4.9 | 114 | V405V (GTC→GTT) | zwf | Glucose‑6‑phosphate 1‑dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (48/59); new base T (3/4); total (51/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 118 total aligned reads displayed.
GAAGTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACATGGAAATTTGTTGA > USA300TCH1516_ALE/1622009‑1622289 | gaagTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTc > 2:943728/1‑141 (MQ=255)gaagTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTc < 2:405940/141‑1 (MQ=255) aagTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTcc < 2:1071997/141‑1 (MQ=255) gTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCaa > 1:1023138/1‑141 (MQ=255) gTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCaa > 1:44539/1‑141 (MQ=255) cAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGt < 3:456504/141‑1 (MQ=255) cAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGt < 3:97094/141‑1 (MQ=255) cAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGt < 1:765194/141‑1 (MQ=255) cAATGAACTTATACTATGAAACTAATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAAAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGt < 2:859434/141‑1 (MQ=255) aaCTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTAc < 1:1082762/141‑1 (MQ=255) cTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTc < 1:927045/141‑1 (MQ=255) cTTATACTATGAAACTGATAAACTATTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACTGAACCTGTTCAATTGTCTTattc > 2:788888/1‑138 (MQ=255) cTTATACTATGAAACTGATAAACTATTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACTGAACCTGTTCAATTGTCTTattc > 4:21170/1‑138 (MQ=255) ttATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCa < 3:352037/141‑1 (MQ=255) ataCTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAAt > 2:875151/1‑141 (MQ=255) ataCTATGAAACTGATAAACTATTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACTGAACCTGTTCAATTGTCTTATTCAAt > 2:407767/1‑141 (MQ=255) aCTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGa < 2:399538/141‑1 (MQ=255) cTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAg < 1:525690/141‑1 (MQ=255) aTGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAgcg < 1:893319/141‑1 (MQ=255) gAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCt > 4:9338/1‑141 (MQ=255) gAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCt < 1:594330/141‑1 (MQ=255) gAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCt > 1:60011/1‑141 (MQ=255) aCTAATAAACTGGTATATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCaa < 2:753470/141‑1 (MQ=255) tGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGa > 2:177892/1‑141 (MQ=255) tGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGa < 1:89650/141‑1 (MQ=255) gATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGAt > 3:437488/1‑141 (MQ=255) aaCTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATaaaa > 2:234192/1‑141 (MQ=255) aaCTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATaaaa > 2:205467/1‑141 (MQ=255) aGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATAc > 4:76243/1‑141 (MQ=255) aGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGCATCTTTACATCTAAATGCTCAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAACGAGCGCTCAAGATAAAATGAATAc > 4:209425/1‑141 (MQ=255) aGATTCAAACCTATTAGTAAGCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATAc < 1:649773/141‑1 (MQ=255) aTTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACTGAACCTGTTCAATTGTCTTATTCAATGAGCGCTCAAGATAAAATGAATACTg < 4:138571/141‑1 (MQ=255) tCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTa > 3:14120/1‑141 (MQ=255) cAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAg > 1:1022720/1‑141 (MQ=255) aaCCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGAt > 1:44431/1‑141 (MQ=255) aaCCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGAt > 2:693033/1‑141 (MQ=255) cTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCa > 2:713341/1‑141 (MQ=255) cTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCa > 1:865889/1‑141 (MQ=255) tATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCat > 1:891985/1‑141 (MQ=255) tATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCat > 1:1050397/1‑141 (MQ=255) tATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAAATGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCat > 2:1039651/1‑141 (MQ=255) aTTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCata < 3:278787/141‑1 (MQ=255) gTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGaa < 2:26550/141‑1 (MQ=255) aaTCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTAGCGAAACTGAACCTGTTCGATTGTCTTATTCAATGAGCGCTCAAGATAAAAGGAATACTGTAGATGCATATGaaaa < 2:623476/141‑1 (MQ=255) aTCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAAt < 2:12759/141‑1 (MQ=255) aTCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAAt > 4:264750/1‑141 (MQ=255) aTCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAAt > 1:420882/1‑141 (MQ=255) cAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCt < 2:173591/141‑1 (MQ=255) cAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCt < 3:368359/141‑1 (MQ=255) atatCCAACCTACTGAAGGCGTATCTTTACATATAAATGCTAAGAAAAATACACAAGGTATAGAAACAGGACCTGTTCAACTGTCTTACTCAATGAGCGCTCTAGATAAAATGAATACTGTAGATGCACATGAAGATCtat < 2:912738/141‑1 (MQ=255) ccAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTattatt < 2:363306/141‑1 (MQ=255) aaCCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGCTGCATATGAAAATCTATTATTTg > 4:201586/1‑141 (MQ=255) aaCCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTg > 1:280454/1‑141 (MQ=255) ccTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGAt < 3:41729/141‑1 (MQ=255) cTAATGAAGGTGTATCTTTACATCAAAAAAAAAAAAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGAtt < 2:773735/141‑1 (MQ=255) aTGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTc > 2:865873/1‑141 (MQ=255) aTGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTc > 1:395206/1‑141 (MQ=255) tGAAGGTGTATGTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCt < 2:967928/141‑1 (MQ=255) tGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCt < 2:284679/141‑1 (MQ=255) tGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCt < 1:671835/141‑1 (MQ=255) tGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGATCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCt < 1:1084550/141‑1 (MQ=255) gAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCtt < 4:319692/141‑1 (MQ=255) gAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCtt > 3:94553/1‑141 (MQ=255) gAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCtt < 2:23911/141‑1 (MQ=255) ggTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTaaa > 2:664526/1‑141 (MQ=255) gtgtATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAg < 2:649796/141‑1 (MQ=255) gtATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGt < 1:653161/141‑1 (MQ=255) aTCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGa < 1:307853/141‑1 (MQ=255) aTCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGa < 1:725544/141‑1 (MQ=255) aTCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCCCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGa < 1:997680/141‑1 (MQ=255) tCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGAt > 1:984389/1‑141 (MQ=255) ttACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGcc > 1:788482/1‑141 (MQ=255) gaCATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCa < 2:692019/140‑1 (MQ=255) cTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTaaa > 1:274492/1‑140 (MQ=255) cTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAAc > 4:420022/1‑141 (MQ=255) tAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACt > 1:718257/1‑141 (MQ=255) tAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACt < 2:564366/141‑1 (MQ=255) aaaTACTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACtt < 3:108332/141‑1 (MQ=255) aaTGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTc > 2:848917/1‑141 (MQ=255) cTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGc < 2:513389/141‑1 (MQ=255) aGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACt > 2:207428/1‑141 (MQ=255) aaaTACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGga < 4:412939/141‑1 (MQ=255) aaaTACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAACACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTGCACGCACTGGga < 1:263853/141‑1 (MQ=255) acacAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGgaagaa > 3:382513/1‑141 (MQ=255) acacAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGgaagaa > 2:788986/1‑141 (MQ=255) cacaAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAAt > 2:740303/1‑141 (MQ=255) cacaAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAAt < 3:347049/141‑1 (MQ=255) acaAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAAtt < 3:175570/141‑1 (MQ=255) acaAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAAtt < 1:659926/141‑1 (MQ=255) acaAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGATCAAGATAATATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAAtt < 2:529916/141‑1 (MQ=255) aGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTaaa > 1:796104/1‑141 (MQ=255) tATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATc < 1:560306/141‑1 (MQ=255) gAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACa > 1:8486/1‑141 (MQ=255) aaaCAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACAt > 1:337084/1‑141 (MQ=255) aGAACCTGTCCAATTGTCTTACTCAATGTGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACaaattt < 3:403348/141‑6 (MQ=255) aGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACATGGaa < 1:164800/141‑1 (MQ=255) aaCCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACATGGAAAt < 2:51916/141‑1 (MQ=255) ccTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACATGGAAAttt > 4:373078/1‑141 (MQ=255) tCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACATGGAAATttgttg < 4:103562/141‑1 (MQ=255) ccAATTGTCTTACCCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACATGGAAATTTGTTGa > 2:330265/1‑141 (MQ=255) | GAAGTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACATGGAAATTTGTTGA > USA300TCH1516_ALE/1622009‑1622289 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |