Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | USA300TCH1516_ALE | 1,622,140 | A→T | 5.0% | T402T (ACA→ACT) | zwf → | Glucose‑6‑phosphate 1‑dehydrogenase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | USA300TCH1516_ALE | 1,622,140 | 0 | A | T | 5.0% | 380.5 / 6.7 | 119 | T402T (ACA→ACT) | zwf | Glucose‑6‑phosphate 1‑dehydrogenase |
Reads supporting (aligned to +/- strand): ref base A (51/62); new base T (3/3); total (54/65) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01 |
Only 100 of 123 total aligned reads displayed.
GAATTTAAAGAAGTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACATGGAA > USA300TCH1516_ALE/1622000‑1622280 | gAATTTAAAGAAGTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACa < 1:735604/141‑1 (MQ=255) aaTTTAAAGAAGTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAg < 4:69899/141‑1 (MQ=255) aaTTTAAAGAAGTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAg < 4:69825/141‑1 (MQ=255) aTTTAAAGAAGTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGa > 1:803531/1‑141 (MQ=255) aaaGAAGTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCt < 1:587902/141‑1 (MQ=255) aaaGAAGTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGAGTATCTTTACATCTAAATGCTAAGCAAAATACACACGGTATCGACACAGAACCt > 2:1031839/1‑141 (MQ=255) aagaagTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTg > 2:714729/1‑141 (MQ=255) gaagTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTc > 2:943728/1‑141 (MQ=255) gaagTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTc < 2:405940/141‑1 (MQ=255) gTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCaa > 1:44539/1‑141 (MQ=255) gTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCaa > 1:25333/1‑141 (MQ=255) gTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCaa > 1:1023138/1‑141 (MQ=255) cAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGt < 3:456504/141‑1 (MQ=255) cAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGt < 3:97094/141‑1 (MQ=255) aTGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCt < 3:427208/141‑1 (MQ=255) aaCTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTAc < 1:1082762/141‑1 (MQ=255) cTTATACTATGAAACTGATAAACTATTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACTGAACCTGTTCAATTGTCTTattc > 2:788888/1‑138 (MQ=255) ttATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCa < 3:352037/141‑1 (MQ=255) ataCTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAAt > 2:875151/1‑141 (MQ=255) aCTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGa < 2:399538/141‑1 (MQ=255) cTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAg < 1:525690/141‑1 (MQ=255) aTGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAgcg < 1:893319/141‑1 (MQ=255) gAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCt > 4:9338/1‑141 (MQ=255) gAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCt > 2:399748/1‑141 (MQ=255) gAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCt > 1:60011/1‑141 (MQ=255) gAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCt < 1:594330/141‑1 (MQ=255) aCTAATAAACTGGTATATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCaa < 2:753470/141‑1 (MQ=255) tGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGa > 2:177892/1‑141 (MQ=255) tGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGa < 1:89650/141‑1 (MQ=255) gATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGAt > 3:437488/1‑141 (MQ=255) aTAAACTATTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACTGAACCTGTTCAATTGTCTTATTCAATGAGCGCTCAAGATa < 2:817910/141‑1 (MQ=255) aaCTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATaaaa > 2:234192/1‑141 (MQ=255) tAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATa > 1:713672/1‑141 (MQ=255) tAGATTCAAACCGATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTAGATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGAGAAAATGGATa < 4:348451/141‑1 (MQ=255) aGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATAc > 4:76243/1‑141 (MQ=255) aGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGCATCTTTACATCTAAATGCTCAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAACGAGCGCTCAAGATAAAATGAATAc > 4:209425/1‑141 (MQ=255) aGATTCAAACCTATTAGTAAGCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATAc < 1:649773/141‑1 (MQ=255) aTTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACTGAACCTGTTCAATTGTCTTATTCAATGAGCGCTCAAGATAAAATGAATACTg < 4:138571/141‑1 (MQ=255) tCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTa > 3:14120/1‑141 (MQ=255) cAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAg > 1:1022720/1‑141 (MQ=255) aaCCTATTAGTAATCAATATCCAACCTAATGGAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGAt > 2:263853/1‑141 (MQ=255) aaCCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGAt > 2:693033/1‑141 (MQ=255) cTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCa > 1:865889/1‑141 (MQ=255) tATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCat > 1:891985/1‑141 (MQ=255) tATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCat > 1:1050397/1‑141 (MQ=255) tATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAAATGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCat > 2:1039651/1‑141 (MQ=255) aTTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCata < 3:278787/141‑1 (MQ=255) aTCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAAt < 2:12759/141‑1 (MQ=255) aTCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAAt > 1:420882/1‑141 (MQ=255) aTCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAAt > 4:264750/1‑141 (MQ=255) cAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCt < 3:368359/141‑1 (MQ=255) cAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCt < 2:173591/141‑1 (MQ=255) atatCCAACCTACTGAAGGCGTATCTTTACATATAAATGCTAAGAAAAATACACAAGGTATAGAAACAGGACCTGTTCAACTGTCTTACTCAATGAGCGCTCTAGATAAAATGAATACTGTAGATGCACATGAAGATCtat < 2:912738/141‑1 (MQ=255) ccAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTattatt < 2:363306/141‑1 (MQ=255) aaCCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGCTGCATATGAAAATCTATTATTTg > 4:201586/1‑141 (MQ=255) ccTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGAt < 3:41729/141‑1 (MQ=255) cTAATGAAGGTGTATCTTTACATCAAAAAAAAAAAAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGAtt < 2:773735/141‑1 (MQ=255) aTGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTc > 1:395206/1‑141 (MQ=255) aTGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTc > 2:865873/1‑141 (MQ=255) tGAAGGTGTATGTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCt < 2:967928/141‑1 (MQ=255) tGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCt < 2:284679/141‑1 (MQ=255) tGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCt < 1:671835/141‑1 (MQ=255) tGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGATCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCt < 1:1084550/141‑1 (MQ=255) gAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCtt < 4:319692/141‑1 (MQ=255) gAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCtt < 2:23911/141‑1 (MQ=255) gAAGGGGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCtt < 2:585330/141‑1 (MQ=255) ggTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTaaa > 2:664526/1‑141 (MQ=255) gtgtATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAg < 2:649796/141‑1 (MQ=255) gtgtATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTCGATTGTCTTAAAg > 1:962447/1‑141 (MQ=255) gtATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGt < 1:653161/141‑1 (MQ=255) aTCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGa < 1:307853/141‑1 (MQ=255) aTCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGa < 1:725544/141‑1 (MQ=255) aTCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCCCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGa < 1:997680/141‑1 (MQ=255) tCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGAt > 1:984389/1‑141 (MQ=255) ttACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGcc > 1:788482/1‑141 (MQ=255) gaCATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCa < 2:692019/140‑1 (MQ=255) cTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTaaa > 1:274492/1‑140 (MQ=255) tAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACt > 1:718257/1‑141 (MQ=255) tAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACt < 2:564366/141‑1 (MQ=255) aaaTACTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACtt < 3:108332/141‑1 (MQ=255) aaTGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTc > 2:848917/1‑141 (MQ=255) aTGCTAAGAAAAATACACAAGGTATCGAAACAGAGCCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCa < 1:814681/141‑1 (MQ=255) cTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGc < 2:513389/141‑1 (MQ=255) aGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACt > 2:207428/1‑141 (MQ=255) aaaTACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGga < 4:412939/141‑1 (MQ=255) aaaTACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAACACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTGCACGCACTGGga < 1:263853/141‑1 (MQ=255) acacAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGgaagaa > 3:382513/1‑141 (MQ=255) acacAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGgaagaa > 2:788986/1‑141 (MQ=255) cacaAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAAt < 3:347049/141‑1 (MQ=255) cacaAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAAt > 2:740303/1‑141 (MQ=255) acaAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAAtt < 3:175570/141‑1 (MQ=255) acaAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAAtt < 1:397715/141‑1 (MQ=255) acaAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGATCAAGATAATATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAAtt < 2:529916/141‑1 (MQ=255) aGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTaaa > 1:796104/1‑141 (MQ=255) tATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATc < 1:560306/141‑1 (MQ=255) cGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAAc < 2:15003/141‑1 (MQ=255) aaaCAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACAt > 1:337084/1‑141 (MQ=255) aaCAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACATg < 2:713672/141‑1 (MQ=255) aGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACATGGaa > 4:126563/1‑141 (MQ=255) aGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACATGGaa < 1:164800/141‑1 (MQ=255) | GAATTTAAAGAAGTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACATGGAA > USA300TCH1516_ALE/1622000‑1622280 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |