Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,622,140 A→T 5.0% T402T (ACA→ACT zwf → Glucose‑6‑phosphate 1‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,622,1400AT 5.0% 380.5 / 6.7 119T402T (ACA→ACTzwfGlucose‑6‑phosphate 1‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (51/62);  new base T (3/3);  total (54/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 123 total aligned reads displayed.

GAATTTAAAGAAGTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACATGGAA  >  USA300TCH1516_ALE/1622000‑1622280
                                                                                                                                            |                                                                                                                                            
gAATTTAAAGAAGTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACa                                                                                                                                              <  1:735604/141‑1 (MQ=255)
 aaTTTAAAGAAGTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAg                                                                                                                                             <  4:69899/141‑1 (MQ=255)
 aaTTTAAAGAAGTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAg                                                                                                                                             <  4:69825/141‑1 (MQ=255)
  aTTTAAAGAAGTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGa                                                                                                                                            >  1:803531/1‑141 (MQ=255)
      aaaGAAGTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCt                                                                                                                                        <  1:587902/141‑1 (MQ=255)
      aaaGAAGTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGAGTATCTTTACATCTAAATGCTAAGCAAAATACACACGGTATCGACACAGAACCt                                                                                                                                        >  2:1031839/1‑141 (MQ=255)
       aagaagTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTg                                                                                                                                       >  2:714729/1‑141 (MQ=255)
         gaagTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTc                                                                                                                                     >  2:943728/1‑141 (MQ=255)
         gaagTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTc                                                                                                                                     <  2:405940/141‑1 (MQ=255)
            gTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCaa                                                                                                                                  >  1:44539/1‑141 (MQ=255)
            gTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCaa                                                                                                                                  >  1:25333/1‑141 (MQ=255)
            gTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCaa                                                                                                                                  >  1:1023138/1‑141 (MQ=255)
                cAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGt                                                                                                                              <  3:456504/141‑1 (MQ=255)
                cAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGt                                                                                                                              <  3:97094/141‑1 (MQ=255)
                  aTGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCt                                                                                                                            <  3:427208/141‑1 (MQ=255)
                     aaCTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTAc                                                                                                                         <  1:1082762/141‑1 (MQ=255)
                       cTTATACTATGAAACTGATAAACTATTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACTGAACCTGTTCAATTGTCTTattc                                                                                                                       >  2:788888/1‑138 (MQ=255)
                        ttATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCa                                                                                                                      <  3:352037/141‑1 (MQ=255)
                          ataCTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAAt                                                                                                                    >  2:875151/1‑141 (MQ=255)
                            aCTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGa                                                                                                                  <  2:399538/141‑1 (MQ=255)
                             cTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAg                                                                                                                 <  1:525690/141‑1 (MQ=255)
                               aTGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAgcg                                                                                                               <  1:893319/141‑1 (MQ=255)
                                 gAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCt                                                                                                             >  4:9338/1‑141 (MQ=255)
                                 gAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCt                                                                                                             >  2:399748/1‑141 (MQ=255)
                                 gAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCt                                                                                                             >  1:60011/1‑141 (MQ=255)
                                 gAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCt                                                                                                             <  1:594330/141‑1 (MQ=255)
                                    aCTAATAAACTGGTATATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCaa                                                                                                          <  2:753470/141‑1 (MQ=255)
                                      tGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGa                                                                                                        >  2:177892/1‑141 (MQ=255)
                                      tGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGa                                                                                                        <  1:89650/141‑1 (MQ=255)
                                       gATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGAt                                                                                                       >  3:437488/1‑141 (MQ=255)
                                        aTAAACTATTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACTGAACCTGTTCAATTGTCTTATTCAATGAGCGCTCAAGATa                                                                                                      <  2:817910/141‑1 (MQ=255)
                                           aaCTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATaaaa                                                                                                   >  2:234192/1‑141 (MQ=255)
                                                 tAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATa                                                                                             >  1:713672/1‑141 (MQ=255)
                                                 tAGATTCAAACCGATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTAGATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGAGAAAATGGATa                                                                                             <  4:348451/141‑1 (MQ=255)
                                                  aGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATAc                                                                                            >  4:76243/1‑141 (MQ=255)
                                                  aGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGCATCTTTACATCTAAATGCTCAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAACGAGCGCTCAAGATAAAATGAATAc                                                                                            >  4:209425/1‑141 (MQ=255)
                                                  aGATTCAAACCTATTAGTAAGCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATAc                                                                                            <  1:649773/141‑1 (MQ=255)
                                                    aTTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACTGAACCTGTTCAATTGTCTTATTCAATGAGCGCTCAAGATAAAATGAATACTg                                                                                          <  4:138571/141‑1 (MQ=255)
                                                      tCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTa                                                                                        >  3:14120/1‑141 (MQ=255)
                                                       cAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAg                                                                                       >  1:1022720/1‑141 (MQ=255)
                                                         aaCCTATTAGTAATCAATATCCAACCTAATGGAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGAt                                                                                     >  2:263853/1‑141 (MQ=255)
                                                         aaCCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGAt                                                                                     >  2:693033/1‑141 (MQ=255)
                                                            cTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCa                                                                                  >  1:865889/1‑141 (MQ=255)
                                                             tATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCat                                                                                 >  1:891985/1‑141 (MQ=255)
                                                             tATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCat                                                                                 >  1:1050397/1‑141 (MQ=255)
                                                             tATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAAATGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCat                                                                                 >  2:1039651/1‑141 (MQ=255)
                                                              aTTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCata                                                                                <  3:278787/141‑1 (MQ=255)
                                                                     aTCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAAt                                                                         <  2:12759/141‑1 (MQ=255)
                                                                     aTCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAAt                                                                         >  1:420882/1‑141 (MQ=255)
                                                                     aTCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAAt                                                                         >  4:264750/1‑141 (MQ=255)
                                                                       cAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCt                                                                       <  3:368359/141‑1 (MQ=255)
                                                                       cAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCt                                                                       <  2:173591/141‑1 (MQ=255)
                                                                         atatCCAACCTACTGAAGGCGTATCTTTACATATAAATGCTAAGAAAAATACACAAGGTATAGAAACAGGACCTGTTCAACTGTCTTACTCAATGAGCGCTCTAGATAAAATGAATACTGTAGATGCACATGAAGATCtat                                                                     <  2:912738/141‑1 (MQ=255)
                                                                             ccAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTattatt                                                                 <  2:363306/141‑1 (MQ=255)
                                                                               aaCCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGCTGCATATGAAAATCTATTATTTg                                                               >  4:201586/1‑141 (MQ=255)
                                                                                 ccTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGAt                                                             <  3:41729/141‑1 (MQ=255)
                                                                                  cTAATGAAGGTGTATCTTTACATCAAAAAAAAAAAAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGAtt                                                            <  2:773735/141‑1 (MQ=255)
                                                                                     aTGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTc                                                         >  1:395206/1‑141 (MQ=255)
                                                                                     aTGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTc                                                         >  2:865873/1‑141 (MQ=255)
                                                                                      tGAAGGTGTATGTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCt                                                        <  2:967928/141‑1 (MQ=255)
                                                                                      tGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCt                                                        <  2:284679/141‑1 (MQ=255)
                                                                                      tGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCt                                                        <  1:671835/141‑1 (MQ=255)
                                                                                      tGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGATCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCt                                                        <  1:1084550/141‑1 (MQ=255)
                                                                                       gAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCtt                                                       <  4:319692/141‑1 (MQ=255)
                                                                                       gAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCtt                                                       <  2:23911/141‑1 (MQ=255)
                                                                                       gAAGGGGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCtt                                                       <  2:585330/141‑1 (MQ=255)
                                                                                          ggTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTaaa                                                    >  2:664526/1‑141 (MQ=255)
                                                                                           gtgtATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAg                                                   <  2:649796/141‑1 (MQ=255)
                                                                                           gtgtATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTCGATTGTCTTAAAg                                                   >  1:962447/1‑141 (MQ=255)
                                                                                             gtATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGt                                                 <  1:653161/141‑1 (MQ=255)
                                                                                               aTCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGa                                               <  1:307853/141‑1 (MQ=255)
                                                                                               aTCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGa                                               <  1:725544/141‑1 (MQ=255)
                                                                                               aTCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCCCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGa                                               <  1:997680/141‑1 (MQ=255)
                                                                                                tCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGAt                                              >  1:984389/1‑141 (MQ=255)
                                                                                                   ttACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGcc                                           >  1:788482/1‑141 (MQ=255)
                                                                                                    gaCATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCa                                          <  2:692019/140‑1 (MQ=255)
                                                                                                         cTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTaaa                                     >  1:274492/1‑140 (MQ=255)
                                                                                                          tAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACt                                    >  1:718257/1‑141 (MQ=255)
                                                                                                          tAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACt                                    <  2:564366/141‑1 (MQ=255)
                                                                                                           aaaTACTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACtt                                   <  3:108332/141‑1 (MQ=255)
                                                                                                            aaTGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTc                                  >  2:848917/1‑141 (MQ=255)
                                                                                                             aTGCTAAGAAAAATACACAAGGTATCGAAACAGAGCCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCa                                 <  1:814681/141‑1 (MQ=255)
                                                                                                                cTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGc                              <  2:513389/141‑1 (MQ=255)
                                                                                                                   aGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACt                           >  2:207428/1‑141 (MQ=255)
                                                                                                                       aaaTACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGga                       <  4:412939/141‑1 (MQ=255)
                                                                                                                       aaaTACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAACACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTGCACGCACTGGga                       <  1:263853/141‑1 (MQ=255)
                                                                                                                           acacAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGgaagaa                   >  3:382513/1‑141 (MQ=255)
                                                                                                                           acacAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGgaagaa                   >  2:788986/1‑141 (MQ=255)
                                                                                                                            cacaAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAAt                  <  3:347049/141‑1 (MQ=255)
                                                                                                                            cacaAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAAt                  >  2:740303/1‑141 (MQ=255)
                                                                                                                             acaAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAAtt                 <  3:175570/141‑1 (MQ=255)
                                                                                                                             acaAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAAtt                 <  1:397715/141‑1 (MQ=255)
                                                                                                                             acaAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGATCAAGATAATATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAAtt                 <  2:529916/141‑1 (MQ=255)
                                                                                                                                aGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTaaa              >  1:796104/1‑141 (MQ=255)
                                                                                                                                   tATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATc           <  1:560306/141‑1 (MQ=255)
                                                                                                                                      cGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAAc        <  2:15003/141‑1 (MQ=255)
                                                                                                                                        aaaCAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACAt      >  1:337084/1‑141 (MQ=255)
                                                                                                                                         aaCAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACATg     <  2:713672/141‑1 (MQ=255)
                                                                                                                                            aGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACATGGaa  >  4:126563/1‑141 (MQ=255)
                                                                                                                                            aGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACATGGaa  <  1:164800/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GAATTTAAAGAAGTACCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAATATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATCGAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTAGATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCACTGGGAAGAATTAAAATCAACATGGAA  >  USA300TCH1516_ALE/1622000‑1622280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: