BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,504,140	T→A	5.7%	R384R (CGA→CGT)	steT ←	Serine/threonine exchanger SteT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,504,140	0	T	A	5.7%	381.7 / 7.8	121	R384R (CGA→CGT)	steT	Serine/threonine exchanger SteT
Reads supporting (aligned to +/- strand): ref base T (60/54); new base A (4/3); total (64/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 123 total aligned reads displayed.

AACTGGTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑TCCCATTGACATCATGATGATAGCGATTATAAG  >  USA300TCH1516_ALE/1504002‑1504280

aaCTGGTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCg                                                                                                                                             >  1:952006/1‑141 (MQ=255)

aaCTGGTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCg                                                                                                                                             <  2:870133/141‑1 (MQ=255)

 aCTGGTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGt                                                                                                                                            <  4:234666/141‑1 (MQ=255)

 aCTGGTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGt                                                                                                                                            <  1:229978/141‑1 (MQ=255)

  cTGGTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGtt                                                                                                                                           <  2:904203/141‑1 (MQ=255)

    ggTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTcc                                                                                                                                         >  1:650753/1‑141 (MQ=255)

    ggTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTcc                                                                                                                                         >  1:1087433/1‑141 (MQ=255)

    ggTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTcc                                                                                                                                         >  1:192132/1‑141 (MQ=255)

    ggTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTcc                                                                                                                                         >  1:408223/1‑141 (MQ=255)

        taCCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCatat                                                                                                                                     <  2:544815/141‑1 (MQ=255)

          ccAAGTGCTGCTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATAttt                                                                                                                                   >  1:367734/1‑141 (MQ=255)

            aaGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTgg                                                                                                                                 >  2:678084/1‑141 (MQ=255)

             aGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGt                                                                                                                                >  1:793945/1‑141 (MQ=255)

              gTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGtt                                                                                                                               <  1:329296/141‑1 (MQ=255)

              gTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGtt                                                                                                                               >  1:44913/1‑141 (MQ=255)

                  tGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACg                                                                                                                           >  2:529038/1‑141 (MQ=255)

                  tGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACg                                                                                                                           >  4:164144/1‑141 (MQ=255)

                   gTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGt                                                                                                                          <  2:916121/141‑1 (MQ=255)

                   gTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATCAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGACGCTCCATATTTGGTTCACGt                                                                                                                          >  4:123162/1‑141 (MQ=255)

                    ttattaGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGtt                                                                                                                         <  1:41295/141‑1 (MQ=255)

                    ttattaGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGtt                                                                                                                         <  1:638362/141‑1 (MQ=255)

                           aaTTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTaa                                                                                                                  >  3:199976/1‑141 (MQ=255)

                           aaTTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATCAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGACGCTCCATATTTGGTTCACGTTTTCTTaa                                                                                                                  >  1:1050395/1‑141 (MQ=255)

                           aaTTCCAATGATTGATAATATAAAATGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAACGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTaa                                                                                                                  <  4:411540/141‑1 (MQ=255)

                              tCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAat                                                                                                               >  3:406652/1‑141 (MQ=255)

                              tCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAat                                                                                                               <  2:619403/141‑1 (MQ=255)

                                cAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAatta                                                                                                             <  1:124976/141‑1 (MQ=255)

                                cAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAatta                                                                                                             >  2:651244/1‑141 (MQ=255)

                                    gATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTAc                                                                                                         >  1:507770/1‑141 (MQ=255)

                                     aTTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTAcc                                                                                                        <  1:1082750/141‑1 (MQ=255)

                                       tGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGc                                                                                                      <  1:51982/141‑1 (MQ=255)

                                       tGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGc                                                                                                      >  1:751886/1‑141 (MQ=255)

                                          tAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGcaac                                                                                                   >  1:764531/1‑141 (MQ=255)

                                          tAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGcaac                                                                                                   >  2:681587/1‑141 (MQ=255)

                                           aaTATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGcaaca                                                                                                  <  1:724588/141‑1 (MQ=255)

                                           aaTATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGcaaca                                                                                                  <  2:146999/141‑1 (MQ=255)

                                             tataAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACaaa                                                                                                <  2:44913/141‑1 (MQ=255)

                                              ataAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAAt                                                                                               <  3:373424/141‑1 (MQ=255)

                                               taAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATg                                                                                              <  4:364818/141‑1 (MQ=255)

                                                aaaTTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACCAATGa                                                                                             <  4:116096/141‑1 (MQ=255)

                                                 aaTTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGAc                                                                                            >  2:393951/1‑141 (MQ=255)

                                                 aaTTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGAc                                                                                            >  2:566191/1‑141 (MQ=255)

                                                 aaTTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGAc                                                                                            <  3:356365/141‑1 (MQ=255)

                                                   ttGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACAt                                                                                          <  2:550179/141‑1 (MQ=255)

                                                     gtgtAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATAc                                                                                        >  1:498133/1‑141 (MQ=255)

                                                       ttAAACAGTGTATTAATTAATACAAATGATACTGCCAAAATAGAAATTAAAGGTATGAACGGATATAAAGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACaa                                                                                      <  4:429788/140‑1 (MQ=255)

                                                       gtAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGGCATACaa                                                                                      <  4:143643/141‑1 (MQ=255)

                                                       gtAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACaa                                                                                      >  3:278280/1‑141 (MQ=255)

                                                        tAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAAt                                                                                     >  2:190905/1‑141 (MQ=255)

                                                        tAAACAGTGTATTAATCAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGACGCTCCATATTTGGTTCACGTTTTCTTAAAATTATTACAGCAACAAATGACATACAAt                                                                                     <  4:113951/141‑1 (MQ=255)

                                                         aaaCAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATa                                                                                    >  4:77405/1‑141 (MQ=255)

                                                          aaCAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAg                                                                                   >  2:452120/1‑141 (MQ=255)

                                                           aCAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGa                                                                                  <  4:365310/141‑1 (MQ=255)

                                                              gtgtATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGaaca                                                                               <  2:952006/141‑1 (MQ=255)

                                                               tgtATTAATTAAAACGAATGGTCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGaacaa                                                                              >  4:204531/1‑141 (MQ=255)

                                                               tgtATTAATCAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGACGCTCCATATTTGGTTCACGTTTTCTTAAAATTATTACAGCAACAAATGACATACAATAGaacaa                                                                              >  1:367876/1‑141 (MQ=255)

                                                                  attaattaATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCa                                                                           >  2:241012/1‑141 (MQ=255)

                                                                   ttaattaaTACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCaa                                                                          <  3:361371/141‑1 (MQ=255)

                                                                   ttaattaaTACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCaa                                                                          >  2:145591/1‑141 (MQ=255)

                                                                   ttaattaaTACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCaa                                                                          >  1:415225/1‑141 (MQ=255)

                                                                           tACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACaaa                                                                  >  2:285653/1‑141 (MQ=255)

                                                                            aCAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAg                                                                 >  4:124308/1‑141 (MQ=255)

                                                                            aCAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAg                                                                 <  3:127766/141‑1 (MQ=255)

                                                                               aaTGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGAtt                                                              <  1:815184/141‑1 (MQ=255)

                                                                               aaTGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGAtt                                                              <  3:440193/141‑1 (MQ=255)

                                                                                aTGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTa                                                             >  1:318215/1‑141 (MQ=255)

                                                                                aTGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTa                                                             >  2:424227/1‑141 (MQ=255)

                                                                                   aTCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACa                                                          >  3:37701/1‑141 (MQ=255)

                                                                                   aTCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACa                                                          <  1:811994/141‑1 (MQ=255)

                                                                                   aTCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGACGCTCCATATTTGGTTCACGtt                                                                                                                         <  2:755239/78‑1 (MQ=255)

                                                                                     ccTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACata                                                        <  4:372941/141‑1 (MQ=255)

                                                                                     ccTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACata                                                        <  2:872088/141‑1 (MQ=255)

                                                                                      cTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACatat                                                       >  1:534391/1‑141 (MQ=255)

                                                                                      cTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACatat                                                       >  1:998499/1‑141 (MQ=255)

                                                                                          cAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGt                                                   <  2:276057/141‑1 (MQ=255)

                                                                                             aaTAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTAGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTATAAt                                                >  4:416071/1‑141 (MQ=255)

                                                                                                 gCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTa                                            >  2:946546/1‑141 (MQ=255)

                                                                                                 gCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTa                                            >  2:1031004/1‑141 (MQ=255)

                                                                                                 gCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTa                                            >  3:441035/1‑141 (MQ=255)

                                                                                                   aaTTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATc                                          <  3:89416/141‑1 (MQ=255)

                                                                                                             aTGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCACATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑Tccc                               <  1:28906/141‑1 (MQ=255)

                                                                                                             aTGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑Tccc                               <  1:199642/141‑1 (MQ=255)

                                                                                                             aTGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑Tccc                               <  3:189423/141‑1 (MQ=255)

                                                                                                                aTCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑TCCCAtt                            <  2:609532/141‑1 (MQ=255)

                                                                                                                  cGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑TCCCATTGa                          >  2:346584/1‑141 (MQ=255)

                                                                                                                    gATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑TCCCATTGAca                        >  3:341654/1‑141 (MQ=255)

                                                                                                                    gATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑TCCCATTGAca                        >  2:846250/1‑141 (MQ=255)

                                                                                                                      tataACGGTACTTTATATGGACGCTCCATATTTGGTTCACGTTTTCTTAAAATTATTACAGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATGTTTGTTATTGTATCAAATGC‑TCCCATTGAcatc                      <  2:952616/141‑1 (MQ=255)

                                                                                                                          aCGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑TCCCATTGACATCatga                  <  1:205366/141‑1 (MQ=255)

                                                                                                                          aCGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑TCCCATTGACATCatga                  >  3:192452/1‑141 (MQ=255)

                                                                                                                          aCGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCAAATGC‑TCCCATTGACATCatga                  >  1:1012104/1‑141 (MQ=255)

                                                                                                                           cGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑TCCCATTGACATCatgat                 <  4:398433/141‑1 (MQ=255)

                                                                                                                           cGGTACTCTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATC‑AATGCGCCCCATTGACATCatgat                 <  2:1087433/141‑1 (MQ=255)

                                                                                                                                  ttATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑TCCCATTGACATCATGATGATAGCg          >  3:427120/1‑141 (MQ=255)

                                                                                                                                  ttATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑TCCCATTGACATCATGATGATAGCg          >  1:839624/1‑141 (MQ=255)

                                                                                                                                   tataTGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑TCCCATTGACATCATGATGATAGCGa         <  2:456386/141‑1 (MQ=255)

                                                                                                                                   tataTGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑TCCCATTGACATCATGATGATAGCGa         <  2:408223/141‑1 (MQ=255)

                                                                                                                                   tataTGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑TCCCATTGACATCATGATGATAGCGa         <  2:192132/141‑1 (MQ=255)

                                                                                                                                     tatGGTCGTTCCATATTTGGTTCACGTTTTCTTGAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑TCCCATTGACATCATGATGATAGCGAtt       >  1:562821/1‑141 (MQ=255)

                                                                                                                                        ggTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑TCCCATTGACATCATGATGATAGCGATtata    <  2:803272/141‑1 (MQ=255)

AACTGGTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGC‑TCCCATTGACATCATGATGATAGCGATTATAAG  >  USA300TCH1516_ALE/1504002‑1504280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`