Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,429,273 G→A 5.7% T328T (ACG→ACA ybiT → putative ABC transporter ATP‑binding protein YbiT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,429,2730GA 5.7% 411.1 / 8.0 132T328T (ACG→ACAybiTputative ABC transporter ATP‑binding protein YbiT
Reads supporting (aligned to +/- strand):  ref base G (65/58);  new base A (2/6);  total (68/64)
Fisher's exact test for biased strand distribution p-value = 1.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 132 total aligned reads displayed.

CAACAAGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTGAAATGGAACCAGACGAA  >  USA300TCH1516_ALE/1429134‑1429408
                                                                                                                                           |                                                                                                                                       
caacaaGTCGTAAAAAACACCTCGAGAAAATAGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGa                                                                                                                                        >  2:401612/1‑141 (MQ=255)
  acaaGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGAtt                                                                                                                                      <  1:1081037/141‑1 (MQ=255)
  acaaGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGAtt                                                                                                                                      >  1:672409/1‑141 (MQ=255)
   caaGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTg                                                                                                                                     <  1:1033760/141‑1 (MQ=255)
   caaGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTg                                                                                                                                     <  1:719082/141‑1 (MQ=255)
   caaGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTg                                                                                                                                     >  1:683442/1‑141 (MQ=255)
   caaGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTg                                                                                                                                     >  4:174101/1‑141 (MQ=255)
     aGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGAc                                                                                                                                   <  2:945703/141‑1 (MQ=255)
       tCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACgg                                                                                                                                 <  1:904222/141‑1 (MQ=255)
       tCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACgg                                                                                                                                 <  1:567573/141‑1 (MQ=255)
        cGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGt                                                                                                                                <  4:54792/141‑1 (MQ=255)
         gTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTg                                                                                                                               <  2:737849/141‑1 (MQ=255)
         gTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTg                                                                                                                               <  1:206527/141‑1 (MQ=255)
         gTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTg                                                                                                                               <  2:267290/141‑1 (MQ=255)
          tAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGgaga                                                                                                                              <  2:633220/141‑1 (MQ=255)
          tAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGa                                                                                                                              >  2:279877/1‑141 (MQ=255)
          tAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGa                                                                                                                              <  1:875220/141‑1 (MQ=255)
          aaaaaaaCAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGa                                                                                                                              <  2:472001/140‑1 (MQ=255)
             aaaaCAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGaaaa                                                                                                                           <  2:118552/141‑1 (MQ=255)
               aacaacTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAg                                                                                                                         >  1:627437/1‑141 (MQ=255)
                acaacTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGt                                                                                                                        <  4:275489/141‑1 (MQ=255)
                acaacTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGAAGGTGAAAAAGt                                                                                                                        <  4:275488/141‑1 (MQ=255)
                     tCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAg                                                                                                                   >  3:228294/1‑141 (MQ=255)
                       gagaAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGat                                                                                                                 <  2:111781/141‑1 (MQ=255)
                         gaAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAGTCGGTAATGACTTACTAATCGTTCGAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGataa                                                                                                               <  1:932967/141‑1 (MQ=255)
                           aaaTTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACGCCTGAGCGTGAGATTGGTAACGACTTATTAATCGTTCAAAATCTTTCTAAAACAATTGACGGCGAAAAAGTATTAGataatg                                                                                                             >  2:189526/1‑140 (MQ=255)
                             aTTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATAtt                                                                                                           <  2:211845/141‑1 (MQ=255)
                               tGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATttc                                                                                                         <  1:891554/141‑1 (MQ=255)
                                gAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATttca                                                                                                        >  2:779475/1‑141 (MQ=255)
                                aaaTTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACGCCTGAGCGTGAGATTGGTAACGACTTATTAATCGTTCAAAATCTTTCTAAAACAAAAAAAAAAAAAAAAGTATTAGataatgtatca                                                                                                        <  2:199998/140‑7 (MQ=255)
                                 aaTTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGataat                                                                                                              <  3:296426/134‑1 (MQ=255)
                                 aaTTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGataat                                                                                                              >  4:296426/1‑134 (MQ=255)
                                  aTTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGAAAAAAAAAAAAAAAGTATTAGATAATATttcatt                                                                                                      <  2:358202/141‑1 (MQ=255)
                                     aGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTcac                                                                                                   >  4:135777/1‑141 (MQ=255)
                                     aGATGATATTCAACCATCATCAAGAAGATATCCTTTCGATAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTcac                                                                                                   >  1:575996/1‑141 (MQ=255)
                                       atgatATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACaa                                                                                                 >  4:37001/1‑141 (MQ=255)
                                        tgatATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAAt                                                                                                <  1:101113/141‑1 (MQ=255)
                                        tgatATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAAt                                                                                                <  1:334100/141‑1 (MQ=255)
                                          atatTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGa                                                                                              >  2:552735/1‑141 (MQ=255)
                                            atTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAAt                                                                                            >  3:204369/1‑141 (MQ=255)
                                               cAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACGCCTGAGCGTGAGATTGGTAACGACTTATTAATCGTTCAAAATCTTTCTAAAACAATTGACGGCGAAAAAGTATTAGATAATGTATCATTCACAATGAATCCa                                                                                         <  3:408802/141‑1 (MQ=255)
                                               cAACCAGCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAGGTATTAGATAATATTTCATTCACAATGAATCCa                                                                                         <  4:306316/141‑1 (MQ=255)
                                                  ccATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAAt                                                                                      >  4:291834/1‑141 (MQ=255)
                                                      catcaAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATa                                                                                  >  2:1059263/1‑141 (MQ=255)
                                                      catcaAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATa                                                                                  >  2:574568/1‑141 (MQ=255)
                                                         caAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAg                                                                               >  1:234166/1‑141 (MQ=255)
                                                              agaTATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAAtt                                                                          <  1:154796/141‑1 (MQ=255)
                                                               gaTATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAAttt                                                                         <  1:490401/141‑1 (MQ=255)
                                                                atatCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAAtttt                                                                        <  1:244848/141‑1 (MQ=255)
                                                                  atCCTTTCGTTAAATTCACGCCTGAGCGTGAGATTGGTAACGACTTATTAATCGTTCAAAATCTTTCTAAAACAATTGACGGCGAAAAAGTATTAGATAATGTATCATTCACAATGAATCCAAATGATAAAGCGATTTTaa                                                                      <  1:848168/141‑1 (MQ=255)
                                                                   tCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAAt                                                                     >  2:259668/1‑141 (MQ=255)
                                                                    ccTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAAtt                                                                    <  2:902480/141‑1 (MQ=255)
                                                                     cTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTg                                                                   >  2:86066/1‑141 (MQ=255)
                                                                     cTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTg                                                                   >  4:75560/1‑141 (MQ=255)
                                                                        tCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTgggg                                                                >  1:352392/1‑141 (MQ=255)
                                                                          gTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCACTTTTAATTGGGGAt                                                              >  1:484736/1‑141 (MQ=255)
                                                                              aaTTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTg                                                          >  1:229797/1‑141 (MQ=255)
                                                                              aaTTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTg                                                          >  1:822838/1‑141 (MQ=255)
                                                                              aaTTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTg                                                          <  2:924553/141‑1 (MQ=255)
                                                                              aaTTCACACATGAGCGCGAAATGGGTGATCACTAACTAATCGTTGAGAAGCACTCTGAAAACATTGACGGGGAAAGAGTAGTGGATAATATGACATTCACAATGAATCCAAATGACAAAGCACTTATAATCgggaaaaatg                                                          >  4:178613/1‑134 (MQ=255)
                                                                               aTTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGa                                                         >  1:871347/1‑141 (MQ=255)
                                                                                 tCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGaaa                                                       <  1:515204/141‑1 (MQ=255)
                                                                                  cacaCCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAAt                                                      <  3:392553/141‑1 (MQ=255)
                                                                                   acacCTTAGCGCGCAATCGGTAATGACAAACTAATCGCTCAAAATCTATCTAAAACGATTGACAGAGAAAAAGTATTAAATAATATTTCATTCACAATGAATCCAAATGATAAAGCAAGTTTACTTGGGGATAGTGAAAtt                                                     >  4:313397/1‑141 (MQ=255)
                                                                                   acacCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAAtt                                                     >  2:689660/1‑141 (MQ=255)
                                                                                   acacCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAAtt                                                     >  1:67312/1‑141 (MQ=255)
                                                                                    cacCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTg                                                    >  1:1071463/1‑141 (MQ=255)
                                                                                     acCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGc                                                   >  1:542789/1‑141 (MQ=255)
                                                                                       cTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGa                                                 >  2:42286/1‑141 (MQ=255)
                                                                                       cTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGa                                                 >  1:160609/1‑141 (MQ=255)
                                                                                                aaaTCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACAt                                        <  1:8644/141‑1 (MQ=255)
                                                                                                aaaTCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACAt                                        <  1:984905/141‑1 (MQ=255)
                                                                                                aaaTCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGCGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACAt                                        <  2:321206/141‑1 (MQ=255)
                                                                                                 aaTCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACAtt                                       >  4:441569/1‑141 (MQ=255)
                                                                                                   tCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGc                                     <  3:81506/141‑1 (MQ=255)
                                                                                                   tCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGc                                     <  3:81528/141‑1 (MQ=255)
                                                                                                    cGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCt                                    >  1:495338/1‑141 (MQ=255)
                                                                                                    cGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTACATTCACAATGAATACAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCt                                    >  2:772499/1‑141 (MQ=255)
                                                                                                     ggTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCtt                                   >  4:145349/1‑141 (MQ=255)
                                                                                                     ggTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCtt                                   >  3:457133/1‑141 (MQ=255)
                                                                                                        aaTGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTaaa                                >  3:217169/1‑141 (MQ=255)
                                                                                                        aaTGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAAATTTAATAGGGGATAGTGAAATTGCGAAAACCACATTGCTTaaa                                >  3:128393/1‑141 (MQ=255)
                                                                                                         aTGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTaaaa                               <  2:951223/141‑1 (MQ=255)
                                                                                                               tACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAg                         <  4:146270/141‑1 (MQ=255)
                                                                                                               tACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAg                         >  1:776761/1‑141 (MQ=255)
                                                                                                                aCTAATCGTTCAAAATCTATCTAAAACGATTGACGGCGAAAGAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCACTTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAGAATATTAGc                        >  2:513863/1‑141 (MQ=255)
                                                                                                                   aaTCTTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCgg                     <  4:398140/141‑1 (MQ=255)
                                                                                                                    aTCGTTCAAAATCTATCTAAACCGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTTCTAAAACCACATTGCTTAAAATATTAGCCGGt                    <  1:575220/141‑1 (MQ=255)
                                                                                                                    aTCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGt                    <  2:470882/141‑1 (MQ=255)
                                                                                                                    aTCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGt                    >  2:361221/1‑141 (MQ=255)
                                                                                                                     tCGTTCAAAATCTTTCTAAAACAATTGACGGCGAAAAAGTATTAGATAATGTATCATTCACAATGAATCCAAATGATAAAGCGATTTTAATTGGAGATAGTGAAATTGCAAAAACAACATTACTTAAAATATTAGctggcg                   <  1:613572/141‑6 (MQ=255)
                                                                                                                     tCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTg                   <  2:1005855/141‑1 (MQ=255)
                                                                                                                     tCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTg                   <  4:70894/141‑1 (MQ=255)
                                                                                                                      cGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTGa                  >  1:116740/1‑141 (MQ=255)
                                                                                                                       gTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTGaa                 >  1:317030/1‑141 (MQ=255)
                                                                                                                          cAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTGAAATg              >  4:175153/1‑141 (MQ=255)
                                                                                                                             aaTCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTGAAATGGaa           >  1:1051524/1‑141 (MQ=255)
                                                                                                                                 tatctaAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTGAAATGGAACCAg       >  1:18693/1‑141 (MQ=255)
                                                                                                                                 tatctaAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTGAAATGGAACCAg       >  3:311174/1‑141 (MQ=255)
                                                                                                                                     taAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTGAAATGGAACCAGACGa   >  2:641598/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                       
CAACAAGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTGAAATGGAACCAGACGAA  >  USA300TCH1516_ALE/1429134‑1429408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: