Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,429,264 A→T 5.5% L325L (CTA→CTT ybiT → putative ABC transporter ATP‑binding protein YbiT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,429,2640AT 5.5% 393.2 / 9.6 128L325L (CTA→CTTybiTputative ABC transporter ATP‑binding protein YbiT
Reads supporting (aligned to +/- strand):  ref base A (60/60);  new base T (2/5);  total (63/65)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01

Only 100 of 137 total aligned reads displayed.

TCTAAACAAGCAACAAGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTGAAATGGAACCAG  >  USA300TCH1516_ALE/1429124‑1429403
                                                                                                                                            |                                                                                                                                           
  taaaCAAGCAACAAGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAatctatc                                                                                                                                           <  1:72972/141‑1 (MQ=255)
  taaaCAAGCAACAAGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAatctatc                                                                                                                                           >  3:354622/1‑141 (MQ=255)
      cAAGCAACAAGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTaaa                                                                                                                                       >  4:13960/1‑141 (MQ=255)
      cAAGCAACAAGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATCACTTACTAATCGTTCAAAATCTATCTaaa                                                                                                                                       <  2:48554/141‑1 (MQ=255)
        aGCAACAAGTCGTAAAAAACAACTCGTGAAAATTGAAGTAGAGGATAGTCGACCACCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAAc                                                                                                                                     >  3:409888/1‑141 (MQ=255)
        aGCAACAAGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAAc                                                                                                                                     <  4:192067/141‑1 (MQ=255)
        aGCAACAAGTCGTAAAAAACAACTCGAGAAAATTCAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAAc                                                                                                                                     <  1:1004774/141‑1 (MQ=255)
            acaaGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGAtt                                                                                                                                 <  4:147229/141‑1 (MQ=255)
            acaaGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGAtt                                                                                                                                 >  1:672409/1‑141 (MQ=255)
            acaaGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGAtt                                                                                                                                 <  1:1081037/141‑1 (MQ=255)
            acaaGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGAtt                                                                                                                                 <  1:82787/141‑1 (MQ=255)
             caaGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTg                                                                                                                                >  4:174101/1‑141 (MQ=255)
             caaGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTg                                                                                                                                >  1:683442/1‑141 (MQ=255)
             caaGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTg                                                                                                                                <  1:719082/141‑1 (MQ=255)
             caaGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTg                                                                                                                                <  1:1033760/141‑1 (MQ=255)
              aaGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGa                                                                                                                               >  1:626926/1‑141 (MQ=255)
               aGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGAc                                                                                                                              <  1:369278/141‑1 (MQ=255)
               aGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGAc                                                                                                                              <  2:945703/141‑1 (MQ=255)
                gTCGTAAAAAACAACTTGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACGCCTGAGCGTGAGATTGGTAACGACTTATTAATCGTTCAAAATCTTTCTAAAACAATTGACg                                                                                                                             >  4:47267/1‑141 (MQ=255)
                 tCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACgg                                                                                                                            <  1:567573/141‑1 (MQ=255)
                 tCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACgg                                                                                                                            <  1:904222/141‑1 (MQ=255)
                   gTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTg                                                                                                                          <  2:737849/141‑1 (MQ=255)
                   gTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTg                                                                                                                          <  2:267290/141‑1 (MQ=255)
                   gTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTg                                                                                                                          <  1:206527/141‑1 (MQ=255)
                    tAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGgaga                                                                                                                         <  2:633220/141‑1 (MQ=255)
                    tAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGa                                                                                                                         <  1:875220/141‑1 (MQ=255)
                    tAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGa                                                                                                                         >  2:279877/1‑141 (MQ=255)
                    aaaaaaaCAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGa                                                                                                                         <  2:472001/140‑1 (MQ=255)
                       aaaaCAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGaaaa                                                                                                                      <  1:22444/141‑1 (MQ=255)
                       aaaaCAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGaaaa                                                                                                                      <  2:118552/141‑1 (MQ=255)
                         aacaacTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAg                                                                                                                    >  1:872668/1‑141 (MQ=255)
                          acaacTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGAAGGTGAAAAAGt                                                                                                                   <  4:275488/141‑1 (MQ=255)
                               tCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAg                                                                                                              >  3:228294/1‑141 (MQ=255)
                                 gagaAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGat                                                                                                            <  2:111781/141‑1 (MQ=255)
                                   gaAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAACTCACACCTGAGCGCGAAGTCGGTAATGACTTACTAATCGTTCTAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGataa                                                                                                          <  1:932670/141‑1 (MQ=255)
                                       aTTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATAtt                                                                                                      <  2:211845/141‑1 (MQ=255)
                                          aaaTTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACGCCTGAGCGTGAGATTGGTAACGACTTATTAATCGTTCAAAATCTTTCTAAAACAAAAAAAAAAAAAAAAGTATTAGataatgtatca                                                                                                   <  2:199998/140‑7 (MQ=255)
                                           aaTTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGataat                                                                                                         <  3:296426/134‑1 (MQ=255)
                                            aTTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGAAAAAAAAAAAAAAAGTATTAGATAATATttcatt                                                                                                 <  2:358202/141‑1 (MQ=255)
                                               aGATGATATTCAACCATCATCAAGAAGATATCCTTTCGATAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTcac                                                                                              >  1:575996/1‑141 (MQ=255)
                                                  tgatATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAAt                                                                                           >  2:940710/1‑141 (MQ=255)
                                                  tgatATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAAt                                                                                           <  1:101113/141‑1 (MQ=255)
                                                  tgatATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAAt                                                                                           <  1:334100/141‑1 (MQ=255)
                                                      atTTAACCATCATCAAGAAGATATAAATTAGTTAAATTGACACCTGAGCGGGAAACCGGTAAAGAGTTAATAATCGTTCAAAAGCTATCTAAAACAAAAAAAAAAAAAAAAGTATTAGATAATATTTCATTCACAATGAAt                                                                                       <  4:352039/141‑1 (MQ=255)
                                                      atTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAAt                                                                                       >  3:197769/1‑141 (MQ=255)
                                                      atTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAAt                                                                                       >  3:204369/1‑141 (MQ=255)
                                                         cAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACGCCTGAGCGTGAGATTGGTAACGACTTATTAATCGTTCAAAATCTTTCTAAAACAATTGACGGCGAAAAAGTATTAGATAATGTATCATTCACAATGAATCCa                                                                                    <  3:408802/141‑1 (MQ=255)
                                                         cAACCAGCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAGGTATTAGATAATATTTCATTCACAATGAATCCa                                                                                    <  4:306316/141‑1 (MQ=255)
                                                            ccATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAAt                                                                                 >  4:291834/1‑141 (MQ=255)
                                                                catcaAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATa                                                                             >  2:574568/1‑141 (MQ=255)
                                                                   caAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAg                                                                          >  4:292272/1‑141 (MQ=255)
                                                                   caAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAg                                                                          >  1:234166/1‑141 (MQ=255)
                                                                        agaTATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAAtt                                                                     <  1:154796/141‑1 (MQ=255)
                                                                         gaTATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAAttt                                                                    <  1:490401/141‑1 (MQ=255)
                                                                          atatCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAAtttt                                                                   <  1:244848/141‑1 (MQ=255)
                                                                            atCCTTTCGTTAAATTCACGCCTGAGCGTGAGATTGGTAACGACTTATTAATCGTTCAAAATCTTTCTAAAACAATTGACGGCGAAAAAGTATTAGATAATGTATCATTCACAATGAATCCAAATGATAAAGCGATTTTaa                                                                 <  1:848168/141‑1 (MQ=255)
                                                                             tCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAAt                                                                >  2:259668/1‑141 (MQ=255)
                                                                              ccTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAAtt                                                               >  3:435963/1‑141 (MQ=255)
                                                                               cTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTg                                                              >  2:86066/1‑141 (MQ=255)
                                                                               cTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTg                                                              >  4:75560/1‑141 (MQ=255)
                                                                                  tCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTgggg                                                           >  1:352392/1‑141 (MQ=255)
                                                                                     ttAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATa                                                        >  1:25587/1‑141 (MQ=255)
                                                                                        aaTTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTg                                                     <  2:924553/141‑1 (MQ=255)
                                                                                        aaTTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTg                                                     >  1:822838/1‑141 (MQ=255)
                                                                                         aTTCACACTTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCAGAATGAATCGAAATGATAAAGCAATTTTAATTTGGGATAGTGa                                                    >  2:698921/1‑141 (MQ=255)
                                                                                         aTTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGa                                                    >  1:871347/1‑141 (MQ=255)
                                                                                             acacCTTAGCGCGCAATCGGTAATGACAAACTAATCGCTCAAAATCTATCTAAAACGATTGACAGAGAAAAAGTATTAAATAATATTTCATTCACAATGAATCCAAATGATAAAGCAAGTTTACTTGGGGATAGTGAAAtt                                                >  4:313397/1‑141 (MQ=255)
                                                                                             acacCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAAtt                                                >  1:67312/1‑141 (MQ=255)
                                                                                              cacCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTg                                               >  1:1071463/1‑141 (MQ=255)
                                                                                               acCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGc                                              >  1:542789/1‑141 (MQ=255)
                                                                                                 cTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGa                                            >  2:42286/1‑141 (MQ=255)
                                                                                                 cTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGa                                            >  1:160609/1‑141 (MQ=255)
                                                                                                          aaaTCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACAt                                   <  1:984905/141‑1 (MQ=255)
                                                                                                          aaaTCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACAt                                   >  1:571792/1‑141 (MQ=255)
                                                                                                          aaaTCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGCGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACAt                                   <  2:321206/141‑1 (MQ=255)
                                                                                                           aaTCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACAtt                                  >  4:441569/1‑141 (MQ=255)
                                                                                                             tCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGc                                <  3:81528/141‑1 (MQ=255)
                                                                                                             tCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGc                                <  3:81506/141‑1 (MQ=255)
                                                                                                              cGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCt                               >  1:495338/1‑141 (MQ=255)
                                                                                                              cGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTACATTCACAATGAATACAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCt                               >  2:772499/1‑141 (MQ=255)
                                                                                                               ggTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCtt                              >  4:145349/1‑141 (MQ=255)
                                                                                                                 tAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTaa                            <  3:249980/141‑1 (MQ=255)
                                                                                                                  aaTGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAAATTTAATAGGGGATAGTGAAATTGCGAAAACCACATTGCTTaaa                           >  3:128393/1‑141 (MQ=255)
                                                                                                                          aCTAATCGTTCAAAATCTATCTAAAACGATTGACGGCGAAAGAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCACTTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAGAATATTAGc                   >  2:513863/1‑141 (MQ=255)
                                                                                                                             aaTCTTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCgg                <  4:398140/141‑1 (MQ=255)
                                                                                                                             aaTCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCgg                <  4:129289/141‑1 (MQ=255)
                                                                                                                              aTCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGt               >  2:361221/1‑141 (MQ=255)
                                                                                                                              aTCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGt               <  2:470882/141‑1 (MQ=255)
                                                                                                                               tCGTTCAAAATCTTTCTAAAACAATTGACGGCGAAAAAGTATTAGATAATGTATCATTCACAATGAATCCAAATGATAAAGCGATTTTAATTGGAGATAGTGAAATTGCAAAAACAACATTACTTAAAATATTAGctggcg              <  1:613572/141‑6 (MQ=255)
                                                                                                                               tCGTTCAAAATCTTTCTAAAACAAAAAAAAAAAAAAAAGTATTAGATAATGTATCATTCACAATGAATCCAAATGATAAAGCGATTTTAATTGGAGATAGTGAAATTGCAAAAACAACATTACTTAAAATATTAGctggcg              <  2:792299/141‑6 (MQ=255)
                                                                                                                               tCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTg              <  2:1005855/141‑1 (MQ=255)
                                                                                                                               tCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTg              <  4:70894/141‑1 (MQ=255)
                                                                                                                                 gTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATTTTAGCCGGTGaa            <  2:5925/141‑1 (MQ=255)
                                                                                                                                   tCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTGAAAt          >  1:1075504/1‑141 (MQ=255)
                                                                                                                                    cAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTGAAATg         >  4:175153/1‑141 (MQ=255)
                                                                                                                                     aaaaTCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTGAAATgg        <  1:159902/141‑1 (MQ=255)
                                                                                                                                        atctatctAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTGAAATGGAAc     >  2:16221/1‑141 (MQ=255)
                                                                                                                                          ctatctaAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTGAAATGGAACCa   >  4:184544/1‑141 (MQ=255)
                                                                                                                                          ctatctaAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTGAAATGGAACCa   >  2:1047007/1‑141 (MQ=255)
                                                                                                                                           tatctaAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTGAAATGGAACCAg  >  3:311174/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TCTAAACAAGCAACAAGTCGTAAAAAACAACTCGAGAAAATTGAATTAGATGATATTCAACCATCATCAAGAAGATATCCTTTCGTTAAATTCACACCTGAGCGCGAAATCGGTAATGACTTACTAATCGTTCAAAATCTATCTAAAACGATTGACGGTGAAAAAGTATTAGATAATATTTCATTCACAATGAATCCAAATGATAAAGCAATTTTAATTGGGGATAGTGAAATTGCGAAAACCACATTGCTTAAAATATTAGCCGGTGAAATGGAACCAG  >  USA300TCH1516_ALE/1429124‑1429403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: