BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,346,067	T→C	5.6%	intergenic (+290/+259)	USA300HOU_RS06685 → / ← ltaE	hypothetical protein/Low specificity L‑threonine aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,346,067	0	T	C	5.6%	320.9 / 6.7	106	intergenic (+290/+259)	USA300HOU_RS06685/ltaE	hypothetical protein/Low specificity L‑threonine aldolase
Reads supporting (aligned to +/- strand): ref base T (39/61); new base C (3/3); total (42/64)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 106 total aligned reads displayed.

TGATGCACACCTATAAAAATCTTAATCATCGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAAT  >  USA300TCH1516_ALE/1345929‑1346205

tgatgCACACCTATAAAAATCTTAATCATCGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTAc                                                                                                                                          >  4:413951/1‑141 (MQ=255)

 gatgCACACCTATAAAAATCTTAATCATCGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACt                                                                                                                                         >  2:706152/1‑141 (MQ=255)

     cacaCCTATAAAAATCTTAATCATCGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCa                                                                                                                                     >  1:991958/1‑141 (MQ=255)

          cTATAAAAATCTTAAACATAGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATaaaa                                                                                                                                <  2:376679/141‑1 (MQ=255)

           tataAAAATCTTAATCATCGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAAt                                                                                                                               <  2:880084/141‑1 (MQ=255)

           tataAAAATCTTAATCATCGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAAt                                                                                                                               <  2:880118/141‑1 (MQ=255)

            atatAAATCTTAATCATCGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATa                                                                                                                              <  2:945654/141‑1 (MQ=255)

            ataAAAATCTTAATCATCGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATa                                                                                                                              >  1:329421/1‑141 (MQ=255)

              aacaaTCTTAATCATCGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAAc                                                                                                                            <  1:1068728/138‑1 (MQ=255)

                aaaTCTTAATCATCGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTa                                                                                                                          <  1:380830/141‑1 (MQ=255)

                   tcctAATCATCGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTTCATTTAAACTAAACATAGATATTCTTACAAATATATACTTAGTAATTCGATTGTTAATACATCACTTTCATAAAATAACTATaa                                                                                                                       >  2:66421/4‑141 (MQ=255)

                   tCTTAATCATCGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACaa                                                                                                                       <  1:604415/141‑1 (MQ=255)

                   ccatattcatcGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACaa                                                                                                                       <  1:817421/135‑1 (MQ=255)

                    cTTAATCATCGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAAc                                                                                                                      >  2:402476/1‑141 (MQ=255)

                    cTTAATCATCGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAAc                                                                                                                      >  2:433767/1‑141 (MQ=255)

                         tcatcGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACcatca                                                                                                                 <  1:55831/141‑1 (MQ=255)

                            tcGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCataa                                                                                                              >  3:118646/1‑141 (MQ=255)

                            tcGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCataa                                                                                                              >  4:166742/1‑141 (MQ=255)

                              gAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCataata                                                                                                            >  1:559928/1‑141 (MQ=255)

                               aaaaGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATAc                                                                                                           <  2:1069780/141‑1 (MQ=255)

                                  aGTCCCAACCATATCCATATTACAAACCTAAATAGTGCCTTCCATTTAAACTAAACATCGATATTCCTACCAATATACCCTTAGTAATCCGCTCGCTAAAACATTCCCTCCACAAACTAACTACCACAATCATACTACTtc                                                                                                        <  2:270113/141‑1 (MQ=255)

                                  aGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTtc                                                                                                        <  1:533199/141‑1 (MQ=255)

                                     cacaAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCTATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCa                                                                                                     <  1:201497/141‑1 (MQ=255)

                                     cacaAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCa                                                                                                     >  3:97718/1‑141 (MQ=255)

                                     cacaAACATCTCCATACTACACACATAAATTGTGCATTCCTTTTAGACTAAACATCGATATTCCGACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCa                                                                                                     <  1:313913/141‑1 (MQ=255)

                                        aaaCATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAAt                                                                                                  >  2:54466/1‑141 (MQ=255)

                                        aaaCATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACCTCTCAAAt                                                                                                  >  2:54695/1‑141 (MQ=255)

                                           cATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATgtc                                                                                               <  2:686777/141‑1 (MQ=255)

                                            aTCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATgtcg                                                                                              <  1:843123/141‑1 (MQ=255)

                                            aTCGCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATgtcg                                                                                              <  4:434577/141‑1 (MQ=255)

                                             tctcCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATgtcgt                                                                                             <  2:194921/141‑1 (MQ=255)

                                             tctcCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATgtcgt                                                                                             <  3:142062/141‑1 (MQ=255)

                                             gctcCATATTAAAAACTTAAATAGTGCATTCCATGTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATgtcgt                                                                                             <  2:894006/140‑1 (MQ=255)

                                               tcCATATTAAAAACTTAAATAGTGCATTTCATTTAAACTAAACATAGATATTCTTACAAATATATACTTAGTAATTCGATTGTTAATACATCACTTTCATAAAATAACTATAACCATCATAATACTTCTCAAATCTCGTCa                                                                                           <  1:98612/141‑1 (MQ=255)

                                                 cATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCaaa                                                                                         <  2:138366/141‑1 (MQ=255)

                                                 cATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCaaa                                                                                         >  1:618811/1‑141 (MQ=255)

                                                 cATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCaaa                                                                                         >  1:618470/1‑141 (MQ=255)

                                                 cATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCaaa                                                                                         >  1:321391/1‑141 (MQ=255)

                                                  atatTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAAt                                                                                        <  2:29488/141‑1 (MQ=255)

                                                   tatTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAAtt                                                                                       >  1:533758/1‑141 (MQ=255)

                                                   tatTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAAtt                                                                                       >  1:533612/1‑141 (MQ=255)

                                                     ttAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTaa                                                                                     >  4:176066/1‑141 (MQ=255)

                                                         aaaCTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTa                                                                                 <  2:355721/141‑1 (MQ=255)

                                                          aaCTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTaa                                                                                >  4:304593/1‑141 (MQ=255)

                                                             ttAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTCCCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAATATTTCTTCATCCATTATATTACTTCTCAAATGTCGGCAAATTAAAGTAAATa                                                                             <  1:285437/141‑1 (MQ=255)

                                                              tAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATAc                                                                            >  1:88080/1‑141 (MQ=255)

                                                                 aTAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGc                                                                         <  2:113192/141‑1 (MQ=255)

                                                                  tAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGca                                                                        <  2:1082230/141‑1 (MQ=255)

                                                                      gCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACaaa                                                                    >  4:208040/1‑141 (MQ=255)

                                                                       cATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAAt                                                                   <  1:403475/141‑1 (MQ=255)

                                                                         ttCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTa                                                                 <  3:405371/141‑1 (MQ=255)

                                                                           ccATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAAc                                                               <  4:410247/141‑1 (MQ=255)

                                                                           ccATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAAc                                                               <  1:6605/141‑1 (MQ=255)

                                                                           ccATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAAc                                                               <  1:671758/141‑1 (MQ=255)

                                                                              tttAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCGCAAATGTCTTCAAATTAAAGTAAATACAGCACAAATTAACAtt                                                            <  1:414640/141‑1 (MQ=255)

                                                                                taaactaaacATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACAtttt                                                          >  2:639927/1‑141 (MQ=255)

                                                                                   actaaacATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATCATCCTTCTAACCTGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTaa                                                       >  3:41042/1‑141 (MQ=255)

                                                                                   actaaacATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTaa                                                       >  1:265815/1‑141 (MQ=255)

                                                                                   actaaacATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTAAAACAATCATCATCATTCCCAAATGTCGCCAAATTAAAGTAAATACAGCACAAATTAACATTTTTaa                                                       >  3:41019/1‑141 (MQ=255)

                                                                                          aTCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAAtatata                                                >  2:320232/1‑141 (MQ=255)

                                                                                           tCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATataa                                               <  2:85197/141‑1 (MQ=255)

                                                                                            cGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATataaa                                              <  4:12739/141‑1 (MQ=255)

                                                                                            cGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATataaa                                              <  1:931818/141‑1 (MQ=255)

                                                                                            cGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTAAAATTAATGTAACTCTAACACAAATTAACATTTTTAAATATataaa                                              <  2:48479/141‑1 (MQ=255)

                                                                                            cGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACAACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATataaa                                              >  3:400922/1‑141 (MQ=255)

                                                                                               tatTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATataaaata                                           >  2:401372/1‑141 (MQ=255)

                                                                                               tagtCCGACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATataaaata                                           >  2:482505/4‑141 (MQ=255)

                                                                                                atTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATataaaataa                                          <  1:54466/141‑1 (MQ=255)

                                                                                                atTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACCTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATataaaataa                                          <  1:54695/141‑1 (MQ=255)

                                                                                                   ccTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAAc                                       <  2:180508/141‑1 (MQ=255)

                                                                                                    cTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACa                                      <  3:107377/141‑1 (MQ=255)

                                                                                                     tACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACaa                                     <  1:964809/141‑1 (MQ=255)

                                                                                                     tACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACaa                                     <  4:420517/141‑1 (MQ=255)

                                                                                                      aCCAATATAGACTTAGTAATTCGATTTTTAATACATTACATTCCTAAAATAAATACATCCATCATAATACTTCTCAAATGTCTTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAg                                    <  4:46837/141‑1 (MQ=255)

                                                                                                       ccAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTACATACAGCACAAATAAACATTTTTAAATATATAAAATAAAACAAGa                                   >  3:225402/1‑141 (MQ=255)

                                                                                                         aaTATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATa                                 >  1:1017898/1‑141 (MQ=255)

                                                                                                          atatatACTTAGTAATTCGATTGTTAATACATCACTTTCATAAAATAACTATAACCATCATAATACTTCTCAAATCTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATAc                                <  2:484242/141‑1 (MQ=255)

                                                                                                          atatAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATAc                                >  1:297008/1‑141 (MQ=255)

                                                                                                            ataGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTc                              <  2:240360/141‑1 (MQ=255)

                                                                                                             taGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCa                             >  3:261149/1‑141 (MQ=255)

                                                                                                              ataCTTAGTAATTCGATTGTTAATACATCACTTTCATAAAATAACTATAACCATCATAATACTTCTCAAATCTCGTCAAATTAAAGTAAATACGGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACtaca                            <  1:478743/139‑4 (MQ=255)

                                                                                                              aGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCaa                            >  3:84723/1‑141 (MQ=255)

                                                                                                              aGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCaa                            <  1:453197/141‑1 (MQ=255)

                                                                                                              aGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCaa                            <  3:276919/141‑1 (MQ=255)

                                                                                                                 cTTAGTAATTCGATTGTTAATACATCACTTTCATAAAATAACTATAACCATCATAATACTTCTCAAATCTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCaataa                         >  2:671660/1‑141 (MQ=255)

                                                                                                                   tAGTAATTCGATTGTTAATACATTACTTTCATAGAATAGCTACGACCATCATAATAGTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATc                       <  1:644260/141‑1 (MQ=255)

                                                                                                                     gTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTg                     >  1:458594/1‑141 (MQ=255)

                                                                                                                           cGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTAc               <  2:438336/141‑1 (MQ=255)

                                                                                                                           cGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTAc               <  1:371647/141‑1 (MQ=255)

                                                                                                                           cGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTAc               <  4:383155/141‑1 (MQ=255)

                                                                                                                           cGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTCCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTAc               <  1:695818/141‑1 (MQ=255)

                                                                                                                             aTTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCt             <  3:273495/141‑1 (MQ=255)

                                                                                                                             aTTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATCAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCt             >  4:315769/1‑141 (MQ=255)

                                                                                                                                gTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGt          <  2:1042032/141‑1 (MQ=255)

                                                                                                                                   aaTACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGtttt       <  2:599781/141‑1 (MQ=255)

                                                                                                                                   aaTACATCACTTTCATAAAATAACTATAACCATCATAATACTTCTCAAATCTCGTCAAAATAAAGAAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACAAAATAATATGTAGTATCTTGtttt       >  3:121214/1‑141 (MQ=255)

                                                                                                                                      aCATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGa    >  1:4469/1‑141 (MQ=255)

                                                                                                                                       cATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGaa   <  4:56009/141‑1 (MQ=255)

                                                                                                                                        aTTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGaat  <  4:227670/141‑1 (MQ=255)

                                                                                                                                        aTTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGaat  <  2:168663/141‑1 (MQ=255)

TGATGCACACCTATAAAAATCTTAATCATCGAAAAGTCACAAACATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAAT  >  USA300TCH1516_ALE/1345929‑1346205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`