BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,341,933	G→A	5.4%	intergenic (+341/‑171)	USA300HOU_RS06615 → / → USA300TCH1516_01244	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,341,933	0	G	A	5.4%	347.5 / 6.1	112	intergenic (+341/‑171)	USA300HOU_RS06615/USA300TCH1516_01244	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (49/56); new base A (4/2); total (54/58)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

Only 100 of 112 total aligned reads displayed.

ATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTAATAATAATAC  >  USA300TCH1516_ALE/1341794‑1342071

aTAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGt                                                                                                                                           <  4:43887/141‑1 (MQ=255)

aTAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGt                                                                                                                                           >  3:47157/1‑141 (MQ=255)

    aaaaGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGa                                                                                                                                       <  2:622039/141‑1 (MQ=255)

     aaaGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAg                                                                                                                                      <  1:893794/141‑1 (MQ=255)

       aGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTg                                                                                                                                    <  3:99036/141‑1 (MQ=255)

       aGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTg                                                                                                                                    <  1:238312/141‑1 (MQ=255)

        gATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGa                                                                                                                                   >  3:53035/1‑141 (MQ=255)

            ggAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGa                                                                                                                               <  3:377929/141‑1 (MQ=255)

            ggAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGa                                                                                                                               >  3:442273/1‑141 (MQ=255)

            ggAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGa                                                                                                                               >  1:288414/1‑141 (MQ=255)

                  gATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATaaa                                                                                                                         >  2:427820/1‑141 (MQ=255)

                    tCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTAAAAGTCGAATTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAaaaaaaaaaaaa                                                                                                                       <  4:208160/141‑1 (MQ=39)

                      gTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAg                                                                                                                     <  2:135506/141‑1 (MQ=255)

                       tCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTTTTTTTTTTTTTTTTTTTGTTCTTTTTCTTTTTTTTTTTTTATCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGTAATAAAAAAGc                                                                                                                    >  4:115792/1‑141 (MQ=255)

                       tCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGc                                                                                                                    >  3:182271/1‑141 (MQ=255)

                            aaaTGCTCCCTTCAAAGTTTTCATTTTTGCAATGTCGACGTCGAAGGGGGCGTTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGGGAAGGAAATAAAAAAGCTCaaa                                                                                                               >  4:329570/1‑141 (MQ=255)

                              aTGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATg                                                                                                             <  1:444294/141‑1 (MQ=255)

                              aTGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATg                                                                                                             <  2:1065953/141‑1 (MQ=255)

                                gCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTa                                                                                                           <  3:205155/141‑1 (MQ=255)

                                gCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTa                                                                                                           >  1:87425/1‑141 (MQ=255)

                                 cTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTAc                                                                                                          >  1:693775/1‑141 (MQ=255)

                                   cccTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCa                                                                                                        >  3:421918/1‑141 (MQ=255)

                                     cTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCaaa                                                                                                      <  2:424719/141‑1 (MQ=255)

                                      ttCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAAt                                                                                                     >  1:941329/1‑141 (MQ=255)

                                      ataaaaGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGAAAAAAAAAAAAAAAAGCTCAAATGTACCAAAt                                                                                                     <  2:754648/138‑1 (MQ=255)

                                         aaaGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGt                                                                                                  <  3:253673/141‑1 (MQ=255)

                                          aaGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGtt                                                                                                 <  1:330067/141‑1 (MQ=255)

                                             ttttCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGCTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCCCAAATGTACCAAATTGTTAAt                                                                                              >  1:1060926/1‑141 (MQ=255)

                                               ttCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCt                                                                                            >  1:1056057/1‑141 (MQ=255)

                                               ttCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCt                                                                                            >  1:1003280/1‑141 (MQ=255)

                                                tCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCtt                                                                                           >  1:533733/1‑141 (MQ=255)

                                                  aTTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTtaa                                                                                         >  1:630735/1‑141 (MQ=255)

                                                   ttttttCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTtaat                                                                                        >  2:875874/1‑141 (MQ=255)

                                                    tttttCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTtaata                                                                                       <  1:538264/141‑1 (MQ=255)

                                                    tttttCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATCGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTtaata                                                                                       >  2:934058/1‑141 (MQ=255)

                                                       ttttttGTCGACAACGCAGGCGGCATTTTCATTAAATTGTTATAGCTTTTTATCTTTGTATAATGAACATATTATTTTTATTTTTTTCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAAt                                                                                    >  2:1080651/6‑141 (MQ=255)

                                                       ttCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAAt                                                                                    <  2:566413/141‑1 (MQ=255)

                                                        tCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAAtc                                                                                   >  2:1084416/1‑141 (MQ=255)

                                                            tGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTa                                                                               >  1:658064/1‑141 (MQ=255)

                                                             gtcgtcTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTAc                                                                              <  2:753710/141‑1 (MQ=255)

                                                             gTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTAc                                                                              <  3:136970/141‑1 (MQ=255)

                                                             gTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTAc                                                                              <  1:207381/141‑1 (MQ=255)

                                                              tCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACt                                                                             >  4:454277/1‑141 (MQ=255)

                                                               cGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACtt                                                                            <  1:885126/141‑1 (MQ=255)

                                                                gtgttCGAAGGGGGCATTTTCATTAAATTGTTGTAGGTGTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGTAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACttt                                                                           <  2:942859/138‑1 (MQ=255)

                                                                  cTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTtat                                                                         >  1:352606/1‑141 (MQ=255)

                                                                   ttCGAAGGGGGCGTTTTCATTAAATTGTTATAGCTTTTTATATTTGTATGATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTtata                                                                        <  2:649145/141‑1 (MQ=255)

                                                                   ttCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTtata                                                                        >  1:185597/1‑141 (MQ=255)

                                                                   ttCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTtata                                                                        <  1:328185/141‑1 (MQ=255)

                                                                    tCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATaa                                                                       <  2:236199/141‑1 (MQ=255)

                                                                     cGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATaaa                                                                      <  2:45979/141‑1 (MQ=255)

                                                                         gggggCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGAtt                                                                  >  1:872028/1‑141 (MQ=255)

                                                                          ggggCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTg                                                                 >  1:1065926/1‑141 (MQ=255)

                                                                          ggggCATTTCCATTATATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGATGAGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTg                                                                 >  2:985215/1‑141 (MQ=255)

                                                                              cATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATg                                                             <  4:403850/141‑1 (MQ=255)

                                                                                 tttCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGAc                                                          >  2:212326/1‑141 (MQ=255)

                                                                                  ttCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACa                                                         <  1:81477/141‑1 (MQ=255)

                                                                                      ttataTTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGATGAGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCg                                                     >  1:85716/5‑141 (MQ=255)

                                                                                      ttAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCg                                                     <  1:646146/141‑1 (MQ=255)

                                                                                       tAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGAGAAAGAAAAAAAAAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGa                                                    <  2:134415/141‑1 (MQ=255)

                                                                                        ataTTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGATGAGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAg                                                   <  2:483140/139‑1 (MQ=255)

                                                                                           ttGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGt                                                >  3:275210/1‑141 (MQ=255)

                                                                                             gTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTa                                              >  4:105970/1‑141 (MQ=255)

                                                                                              ttATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTaa                                             >  2:625314/1‑141 (MQ=255)

                                                                                              ttATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTaa                                             >  1:916562/1‑141 (MQ=255)

                                                                                               tataGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAt                                            >  4:297739/1‑141 (MQ=255)

                                                                                                ataGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAtc                                           <  2:335370/141‑1 (MQ=255)

                                                                                                 taGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAtca                                          <  1:913020/141‑1 (MQ=255)

                                                                                                  aGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAATCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAtcag                                         >  3:354052/1‑141 (MQ=255)

                                                                                                   gCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAtcagt                                        <  2:468720/141‑1 (MQ=255)

                                                                                                       tttATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCagg                                    <  1:20806/141‑1 (MQ=255)

                                                                                                       tttATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCagg                                    <  1:886013/141‑1 (MQ=255)

                                                                                                       tttATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCagg                                    <  2:352606/141‑1 (MQ=255)

                                                                                                       tttATATTTGTATAATGAACATATAAGTTTAAGAAGATGAGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCagg                                    >  3:182714/1‑141 (MQ=255)

                                                                                                       tttATATTTGTATAATGAACATATAAGTTTAAGAAGATGAGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCagg                                    >  2:285706/1‑141 (MQ=255)

                                                                                                          gtatTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAGTGGACATTCGAGCGTTAATCAGTCaggagg                                 <  2:491687/140‑1 (MQ=255)

                                                                                                          atatTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCaggagg                                 >  2:33906/1‑141 (MQ=255)

                                                                                                          atatTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCaggagg                                 <  2:70138/141‑1 (MQ=255)

                                                                                                           tatTTGTATAATGAACATATAAGTTTAAGAAGATGAGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAggg                                <  1:190675/141‑1 (MQ=255)

                                                                                                             tttGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGAc                              >  1:684973/1‑141 (MQ=255)

                                                                                                              ttGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACt                             <  2:217378/141‑1 (MQ=255)

                                                                                                               tGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACtt                            <  1:684333/141‑1 (MQ=255)

                                                                                                                gTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGaaagt                           <  3:238977/141‑5 (MQ=255)

                                                                                                                gTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACttt                           <  4:450547/141‑1 (MQ=255)

                                                                                                                     aTGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACAATCGAGCGTTAATCAGTTAGGAGGGACTTTCactc                      >  2:403135/1‑141 (MQ=255)

                                                                                                                      tGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCcctcc                     >  3:105333/1‑141 (MQ=255)

                                                                                                                       gAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCcctcct                    <  1:315297/141‑1 (MQ=255)

                                                                                                                               aaGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTta            >  2:890315/1‑141 (MQ=255)

                                                                                                                                aGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaa           >  1:271063/1‑141 (MQ=255)

                                                                                                                                  tttAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaata         >  2:512776/1‑141 (MQ=255)

                                                                                                                                    tAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaataat       <  3:424575/141‑1 (MQ=255)

                                                                                                                                    tAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaataat       <  2:630735/141‑1 (MQ=255)

                                                                                                                                    tAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaataat       <  2:985648/141‑1 (MQ=255)

                                                                                                                                    tAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaataat       <  1:82241/141‑1 (MQ=255)

                                                                                                                                      agaagGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaataataa     >  4:25543/1‑141 (MQ=255)

                                                                                                                                      agaagGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaataataa     >  2:600604/1‑141 (MQ=255)

                                                                                                                                       gaagGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTaataataat    >  2:298622/1‑141 (MQ=255)

                                                                                                                                       gaagGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTaataataat    >  1:320746/1‑141 (MQ=255)

                                                                                                                                        aagGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTAataataata   >  1:737256/1‑141 (MQ=255)

                                                                                                                                         agGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTATTCAGTCAGGAGGGACTTTCCCTCCTACAATTTAATAATAATAc  >  4:425258/1‑141 (MQ=255)

ATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTAATAATAATAC  >  USA300TCH1516_ALE/1341794‑1342071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`