Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | USA300TCH1516_ALE | 70,101 | A→T | 5.0% | I228N (ATT→AAT) | argF_1 ← | Ornithine carbamoyltransferase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | USA300TCH1516_ALE | 70,101 | 0 | A | T | 5.0% | 445.9 / 11.9 | 140 | I228N (ATT→AAT) | argF_1 | Ornithine carbamoyltransferase |
Reads supporting (aligned to +/- strand): ref base A (61/72); new base T (3/4); total (64/76) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 |
Only 100 of 141 total aligned reads displayed.
AGCAATGTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATACAAGATGGAAAGT > USA300TCH1516_ALE/69964‑70240 | aGCAATGTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTa < 4:137744/141‑1 (MQ=255) aagTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGTTAAGGTTCTAATAATTTGATACGTTTTTCCCAAACGTCATCAGGTTCACCCATAGATACCCAAACATCTGATGTAATTTACac < 2:922607/139‑2 (MQ=255) aTGTTCAACAATTGTATGCGGATCACATCTTTTTCTGCTTCAATTTTTTCTTACTCGGTATGTTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATc < 1:1024855/141‑1 (MQ=21) aTGTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATNGATACCCAAACANCTG‑TGTAAATTACATc < 3:311517/141‑1 (MQ=255) aTGTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGATGTAATTTACac < 2:801425/141‑2 (MQ=255) aTGTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGATGTAATTTACac < 2:801245/141‑2 (MQ=255) aTGTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGATGTAATTTACac < 2:756828/141‑2 (MQ=255) aTGTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATc < 1:113073/141‑1 (MQ=255) aTGTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATc < 3:18107/141‑1 (MQ=255) tCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGAt > 1:45352/1‑141 (MQ=255) cAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATc < 1:395196/141‑1 (MQ=255) aaaaTTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCt < 3:126235/141‑1 (MQ=255) aaaTTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCtt > 4:430037/1‑141 (MQ=255) aaTTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCttt < 4:75381/141‑1 (MQ=255) aTTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTg > 1:114391/1‑141 (MQ=255) tGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGac < 2:771716/141‑1 (MQ=255) tGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGac > 2:129927/1‑141 (MQ=255) tGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGac > 4:246322/1‑141 (MQ=255) tATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGacac < 2:167638/141‑1 (MQ=255) aTGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACAcc > 3:431847/1‑141 (MQ=255) aTGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACAcc > 1:141502/1‑141 (MQ=255) ttACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTtcatca < 1:178913/141‑1 (MQ=255) gTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATatca > 3:193022/1‑141 (MQ=255) tttttttCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATatcat > 1:267287/1‑141 (MQ=255) tCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTTTTTTTTTTTTTCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACATCTTCATCAATATCATCTGTta > 2:2745/1‑141 (MQ=255) cATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTtat > 1:946915/1‑141 (MQ=255) aaTTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAgg < 2:721304/141‑1 (MQ=255) aaTTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAgg < 4:267318/141‑1 (MQ=255) aTTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGa < 1:142216/141‑1 (MQ=255) ttCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTTTTTTTTTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATTAGGAt > 2:449786/1‑141 (MQ=255) ttCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAggct > 1:469791/1‑139 (MQ=255) aGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTcacc < 2:243604/141‑1 (MQ=255) gTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTcacca < 1:42326/141‑1 (MQ=255) ttACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTcaccac > 3:416375/1‑141 (MQ=255) tACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCaccacc > 1:101566/1‑141 (MQ=255) tACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCaccacc < 4:394621/141‑1 (MQ=255) acacGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCg < 2:601651/141‑1 (MQ=255) acGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACAACAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGtt < 2:539178/141‑1 (MQ=255) gTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCGCCACCGttttt < 1:1074672/141‑1 (MQ=255) gTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGttttt > 4:454984/1‑141 (MQ=255) gTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGttttt < 3:124128/141‑1 (MQ=255) aTGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGt < 4:143102/141‑1 (MQ=255) aTGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGt > 2:246563/1‑141 (MQ=255) ggTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCATCACCGTTTTTGTCa > 2:436211/1‑141 (MQ=255) ttCTAATAATTTGCTACTTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGc < 1:332776/141‑1 (MQ=255) ttCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGc < 2:221320/141‑1 (MQ=255) tCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCt < 1:470171/141‑1 (MQ=255) tCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCt < 1:1066742/141‑1 (MQ=255) cTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCta > 3:332740/1‑141 (MQ=255) taataaTTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCtat < 4:402217/141‑1 (MQ=255) aataatTTGATACGTTTTTTTTTTTTTTTTTCAGGTTCACCCATAGATACCCAAACATCTG‑TGCAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCTTTTTTGTCATCtata > 2:513869/1‑141 (MQ=255) ataatTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTatat < 2:282451/141‑1 (MQ=255) atTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCAt > 1:229068/1‑141 (MQ=255) tttGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCAtt < 3:228605/141‑1 (MQ=255) tGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTac > 2:708982/1‑141 (MQ=255) gATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTaca < 1:835602/141‑1 (MQ=255) gATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTaca > 2:404119/1‑141 (MQ=255) aTACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTacac > 3:48071/1‑141 (MQ=255) tACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACAcc < 1:355487/141‑1 (MQ=255) aCGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCg > 2:325584/1‑141 (MQ=255) gTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGAt < 2:579452/141‑1 (MQ=255) tttttCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTAAGCTATATCATTACACCGAtt > 1:533044/1‑141 (MQ=255) tCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTtaat < 2:278736/141‑1 (MQ=255) tCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTtaat > 2:294750/1‑141 (MQ=255) tCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTtaat < 2:807408/141‑1 (MQ=255) ccAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTtaataa > 4:277620/1‑141 (MQ=255) aaaCTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAAtt < 1:3260/141‑1 (MQ=255) aaCTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTTATCAATATTATCAGTTATAAGGATATCACCACCGTTTTTGTCAGCTATATCATTACACCTTTTTATTAAttt > 4:344840/1‑140 (MQ=255) catcaGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTTTTTTTTTTTTCATTACACCGATTGAATAATTCATCAg > 4:381182/1‑141 (MQ=255) catcaGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAg < 1:825268/141‑1 (MQ=255) catcaGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAg < 2:141502/141‑1 (MQ=255) catcaGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAg < 2:141416/141‑1 (MQ=255) catcaGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAg < 1:960285/141‑1 (MQ=255) atcaGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATCTCATTACACCGATTTAATAATTCATCAGt > 4:307/1‑141 (MQ=255) atcaGGTTCACCCATAGATACCCAAACATCAG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATGTAATAATTCATCAGt > 4:386307/1‑141 (MQ=255) tcaGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGtt > 2:523938/1‑141 (MQ=255) tcaGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGtt > 1:493912/1‑141 (MQ=255) tcaGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGtt > 1:51131/1‑141 (MQ=255) caGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTg > 2:723690/1‑141 (MQ=255) aGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTgg > 4:437680/1‑141 (MQ=255) ttCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGAtt < 1:594467/141‑1 (MQ=255) cACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATGCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTa > 4:112968/1‑141 (MQ=255) aCCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAg < 4:413225/141‑1 (MQ=255) tAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCtt < 1:1022847/141‑1 (MQ=255) tAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCtt > 1:772205/1‑141 (MQ=255) aTACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAg < 3:290515/141‑1 (MQ=255) tACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAgg < 1:524229/141‑1 (MQ=255) cccAAATATCTT‑TGTAAATTACATCATATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGAc < 2:93528/141‑1 (MQ=255) ccAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACa < 4:158567/141‑1 (MQ=255) ccAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACa < 1:736682/141‑1 (MQ=255) aaaCATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATa > 2:559167/1‑141 (MQ=255) aCATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATACa < 2:362374/141‑1 (MQ=255) aTCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATACAAg > 1:130796/1‑141 (MQ=255) tctgatgtAATTTACA‑CAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATACAAGa > 1:477118/6‑141 (MQ=255) tCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATACAAGa < 2:587084/141‑1 (MQ=255) ctgatgtAATTTACA‑CAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATACAAGAt > 1:257326/5‑141 (MQ=255) tg‑tgTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATACAAGATg > 1:225661/1‑141 (MQ=255) tg‑tgTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAACTCATCAGTTGGATTTAGTTCTTTAGGACATACAAGATg < 1:844869/141‑1 (MQ=255) tg‑tgTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACAACGATCTAATACTTCATCAGTTGGATTTAGTACTTTAGGACATACAAGATg < 2:377391/141‑1 (MQ=255) aaTTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATACAAGATGGAAAGt < 1:367602/141‑1 (MQ=255) | AGCAATGTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTG‑TGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATACAAGATGGAAAGT > USA300TCH1516_ALE/69964‑70240 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |