BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,306,583	C→T	7.6%	P216S (CCA→TCA)	phaB →	Acetoacetyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,306,583	0	C	T	7.6%	338.3 / 10.6	112	P216S (CCA→TCA)	phaB	Acetoacetyl‑CoA reductase
Reads supporting (aligned to +/- strand): ref base C (65/38); new base T (3/6); total (68/44)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

TCGCACCTGGATTTGTAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTG  >  USA300TCH1516_ALE/1306446‑1306719

  gCACCTGGATTTGTAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAAt                                                                                                                                     >  2:765980/1‑141 (MQ=255)

  gCACCTGGATTTGTAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACACGCGCCTATTTATATCACTCAAAt                                                                                                                                     <  4:240275/141‑1 (MQ=255)

   cACCTGGATTTGTAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATg                                                                                                                                    >  2:446716/1‑141 (MQ=255)

         gATTTGTAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGaa                                                                                                                              >  1:884214/1‑141 (MQ=255)

          aTTTGTAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAg                                                                                                                             <  1:49685/141‑1 (MQ=255)

          aTTTGTAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAg                                                                                                                             <  2:569440/141‑1 (MQ=255)

            ttGTAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAgtg                                                                                                                           <  4:177304/141‑1 (MQ=255)

             tGTAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAgtgt                                                                                                                          <  1:765980/141‑1 (MQ=255)

               tAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTa                                                                                                                        <  1:936843/141‑1 (MQ=255)

               tAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTa                                                                                                                        >  1:540848/1‑141 (MQ=255)

               tAGCCGGTAATATGGCAAGGGAGTGGCAAGAAGATGAACTTCAAGCAATGACAACTGAGTTACCACAACAGCGAATGGCTTTACCGAGTGAGGTTGCTCATAAATGGGCCTATGTATATCACTCAATAGCTAGAAGCGTTa                                                                                                                        >  2:684482/1‑141 (MQ=255)

                        atatGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGACTTACCACAACAGCGAGTGGGTGTACCGAGTGTGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACta                                                                                                               >  1:319457/1‑141 (MQ=255)

                        atatGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACta                                                                                                               >  2:375113/1‑141 (MQ=255)

                        atatGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACta                                                                                                               >  2:973600/1‑141 (MQ=255)

                          gtGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACTCAAATGCTAGAAGCGTTACTGGAACtata                                                                                                             >  4:442267/2‑141 (MQ=255)

                          atGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACtata                                                                                                             >  3:424364/1‑141 (MQ=255)

                          atGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACtata                                                                                                             >  3:159852/1‑141 (MQ=255)

                           tGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACACGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATAc                                                                                                            >  4:395999/1‑141 (MQ=255)

                            ggCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACa                                                                                                           <  1:380711/141‑1 (MQ=255)

                            ggCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACa                                                                                                           >  2:567712/1‑141 (MQ=255)

                             gCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACTCAAATGCTAGAAGCGTTACTGGAACTATACAg                                                                                                          <  3:69960/141‑1 (MQ=255)

                             gCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAg                                                                                                          <  2:944064/141‑1 (MQ=255)

                                agtgagtgGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGaaa                                                                                                       <  1:790453/141‑1 (MQ=255)

                                 gtgagtgGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAg                                                                                                      >  2:969494/1‑141 (MQ=255)

                                   gagtgGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGtt                                                                                                    >  2:108504/1‑141 (MQ=255)

                                    agtgGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTa                                                                                                   <  2:170679/141‑1 (MQ=255)

                                    agtgGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTa                                                                                                   <  4:106880/141‑1 (MQ=255)

                                           agaagaTGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGc                                                                                            <  4:287526/141‑1 (MQ=255)

                                                 tGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGta                                                                                      >  1:57012/1‑141 (MQ=255)

                                                  gAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGtat                                                                                     >  2:190013/1‑141 (MQ=255)

                                                   aaCTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACTCAAATGCTAGAAGCGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGtata                                                                                    <  1:677556/141‑1 (MQ=255)

                                                   aaCTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGtata                                                                                    >  1:489955/1‑141 (MQ=255)

                                                   aaCTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGtata                                                                                    >  3:243786/1‑141 (MQ=255)

                                                   aaCTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGtata                                                                                    <  1:15042/141‑1 (MQ=255)

                                                   aaCTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGtata                                                                                    >  2:1019494/1‑141 (MQ=255)

                                                   aaCTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGG‑TTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTCTACCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTatat                                                                                   >  1:18934/1‑141 (MQ=255)

                                                    aCTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTatat                                                                                   >  4:190087/1‑141 (MQ=255)

                                                        cAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTaa                                                                               >  2:720479/1‑141 (MQ=255)

                                                        cAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTaa                                                                               <  1:37042/141‑1 (MQ=255)

                                                         aaGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTaaa                                                                              >  2:29396/1‑141 (MQ=255)

                                                           gCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAat                                                                            >  4:286076/1‑141 (MQ=255)

                                                             aaTGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAatat                                                                          >  3:301015/1‑141 (MQ=255)

                                                               tGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAg                                                                        <  1:145736/141‑1 (MQ=255)

                                                               tGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAg                                                                        >  2:968674/1‑141 (MQ=255)

                                                               tGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTCGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGCAAGTTAATGGTGCTTGGTATATTTAAACtataa                                                                        >  4:263416/1‑140 (MQ=255)

                                                                gATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGa                                                                       <  1:137233/141‑1 (MQ=255)

                                                                 aTAACTGAATTACCACAACAGCGATTGGATTTACCGAGTGATGTTGCTCATCCAGGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGaa                                                                      >  2:311822/1‑141 (MQ=255)

                                                                  tAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAAc                                                                     >  1:137871/1‑141 (MQ=255)

                                                                  tAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAAc                                                                     <  3:426602/141‑1 (MQ=255)

                                                                  tAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAAc                                                                     >  1:45460/1‑141 (MQ=255)

                                                                    aCTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACCTGCGCCAATTTATATCAACCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTa                                                                   <  1:917988/141‑1 (MQ=255)

                                                                        aaTTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTAtttt                                                               <  2:187773/141‑1 (MQ=255)

                                                                              cacaACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAg                                                         >  2:666515/1‑141 (MQ=255)

                                                                                caacaGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTACAATATAGAACTATTATTATAAGCa                                                       >  4:191595/1‑141 (MQ=255)

                                                                                caacaGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCa                                                       <  1:2602/141‑1 (MQ=255)

                                                                                 aacaGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCaa                                                      <  3:222125/141‑1 (MQ=255)

                                                                                 aacaGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCaa                                                      >  3:246732/1‑141 (MQ=255)

                                                                                   caGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAAt                                                    >  4:399042/1‑141 (MQ=255)

                                                                                       gATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAACTGttt                                                >  2:215619/1‑141 (MQ=255)

                                                                                         ttGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGTAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAGCTATTTTTATTAGCAAATGttttt                                              <  2:914314/141‑1 (MQ=255)

                                                                                         ttGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGttttt                                              <  1:479477/141‑1 (MQ=255)

                                                                                         ttGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGttttt                                              <  2:356524/141‑1 (MQ=255)

                                                                                          tGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTa                                             <  1:567712/141‑1 (MQ=255)

                                                                                           ggTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCTCCCACATGCTAGCCGTGTTCCAGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTac                                            <  1:1029169/141‑2 (MQ=255)

                                                                                             ttttACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACTCAAATGCTAGAAGCGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTAtga                                          <  2:575272/141‑1 (MQ=255)

                                                                                                tACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAGATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTAtgattg                                       <  2:107224/141‑1 (MQ=255)

                                                                                                tACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTAtgattg                                       <  1:637513/141‑1 (MQ=255)

                                                                                                 aCCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTAtgattga                                      <  3:63861/141‑1 (MQ=255)

                                                                                                  ccGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTAtgattgat                                     >  2:89544/1‑141 (MQ=255)

                                                                                                   cGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACTCAAATGCTAGAAGCGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATg                                    <  2:805180/141‑1 (MQ=255)

                                                                                                   cGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATg                                    <  2:817969/141‑1 (MQ=255)

                                                                                                      gTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTta                                 >  4:62501/1‑141 (MQ=255)

                                                                                                      gTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTta                                 >  2:678695/1‑141 (MQ=255)

                                                                                                         aGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTataa                              >  1:980572/1‑141 (MQ=255)

                                                                                                          ggTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTataat                             >  2:625311/1‑141 (MQ=255)

                                                                                                           gTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAACTGTTTTTATGATTGATGTTataata                            >  3:267842/1‑141 (MQ=255)

                                                                                                           gTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTataata                            <  2:744705/141‑1 (MQ=255)

                                                                                                             tGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATg                          >  2:971732/1‑141 (MQ=255)

                                                                                                             tGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGATAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAAAGTTTTTTTGATTGATGTTATAatatt                          >  2:507928/1‑140 (MQ=255)

                                                                                                              gCTCATACATGCGCCTATTTATATCACTCAAATGCTAGAAGCGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGa                         >  1:980406/1‑141 (MQ=255)

                                                                                                              gCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTAtgattgat                                     >  2:225080/1‑129 (MQ=255)

                                                                                                              gCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTAtgattgat                                     <  1:225080/129‑1 (MQ=255)

                                                                                                              gCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGa                         >  4:47936/1‑141 (MQ=255)

                                                                                                              gCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGa                         <  4:224973/141‑1 (MQ=255)

                                                                                                                tCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAat                       >  1:78048/1‑141 (MQ=255)

                                                                                                                         cgcCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATg              >  2:32014/1‑141 (MQ=255)

                                                                                                                         cgcCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATg              >  4:128461/1‑141 (MQ=255)

                                                                                                                         cgcCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATg              >  4:457111/1‑141 (MQ=255)

                                                                                                                         cgcCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATg              >  1:253619/1‑141 (MQ=255)

                                                                                                                         cgcCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATg              >  2:1031563/1‑141 (MQ=255)

                                                                                                                         cgcCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATg              >  1:995070/1‑141 (MQ=255)

                                                                                                                         cgcCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATg              >  1:675943/1‑141 (MQ=255)

                                                                                                                           ccTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGaa            >  2:688183/1‑141 (MQ=255)

                                                                                                                           ccTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGaa            >  2:300217/1‑141 (MQ=255)

                                                                                                                            cTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAAt           <  1:952735/141‑1 (MQ=255)

                                                                                                                            cTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAAt           <  2:550882/141‑1 (MQ=255)

                                                                                                                              aTTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAGCTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCa         <  2:325213/141‑1 (MQ=255)

                                                                                                                                   tatCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAAtt    >  2:199517/1‑141 (MQ=255)

                                                                                                                                     tCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTg  >  2:866068/1‑141 (MQ=255)

                                                                                                                                     tCACCCAAACGCTAGAAGTGTTACTGGAACTATACATAAAGTAACTGGTGCGTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGCTTTTATGATTGATGTTCTAATATGAATATTACGATGAATCATaactcg  >  2:805067/1‑137 (MQ=255)

TCGCACCTGGATTTGTAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTG  >  USA300TCH1516_ALE/1306446‑1306719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`