| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,250,361 | C→T | 6.1% | A44V (GCA→GTA) | ftsY → | Signal recognition particle receptor FtsY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,250,361 | 0 | C | T | 6.1% | 349.6 / 7.9 | 114 | A44V (GCA→GTA) | ftsY | Signal recognition particle receptor FtsY |
| Reads supporting (aligned to +/- strand): ref base C (68/39); new base T (5/2); total (73/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
AGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATGGGTGCTAAATTTAAAGCA > USA300TCH1516_ALE/1250221‑1250500 | aggagCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGc < 1:888442/141‑1 (MQ=255)aggagCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGc < 2:172001/141‑1 (MQ=255) ggagCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCa < 4:201906/141‑1 (MQ=255) ggagCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCa < 3:392303/141‑1 (MQ=255) ggagCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCa < 3:218103/141‑1 (MQ=255) gagCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCaa > 4:287613/1‑141 (MQ=255) cAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATg > 2:1012213/1‑141 (MQ=255) cAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATg < 2:636150/141‑1 (MQ=255) aatGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGAttt < 1:1023821/141‑1 (MQ=255) aatGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGAttt < 1:329207/141‑1 (MQ=255) tGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAg < 3:344285/141‑1 (MQ=255) tGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAg < 1:778274/141‑1 (MQ=255) gCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTATGAAGGTTCAACGCAGGACGCAAATGATTTAGCAg > 4:382902/1‑141 (MQ=255) cTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGa > 1:435170/1‑141 (MQ=255) ttttttAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGaa < 4:313086/141‑1 (MQ=255) tttttAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGaaa < 2:246601/141‑1 (MQ=255) ttttAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGaaaa < 1:151863/141‑1 (MQ=255) tttAAACGCTTAAATGATATTTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAAt > 2:767331/1‑141 (MQ=255) ttAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATg > 1:719583/1‑141 (MQ=255) ttAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATg > 2:599893/1‑141 (MQ=255) ttAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATg < 4:163416/141‑1 (MQ=255) tAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGc < 2:104817/141‑1 (MQ=255) aaaCGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCt > 1:287988/1‑141 (MQ=255) aaaCGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCt > 2:951133/1‑141 (MQ=255) aCGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGaaaaaaaaaa < 2:658128/141‑7 (MQ=255) cGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGaa > 1:637603/1‑141 (MQ=255) tcTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCAGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAg > 2:249573/2‑141 (MQ=255) cTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACGAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGt < 2:571867/141‑1 (MQ=255) cTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGGCGCAAATGATTTAGCAGAAAATGCTGAAGt < 1:869607/141‑1 (MQ=255) aaGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTAAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGaaaa > 1:10705/1‑141 (MQ=255) aGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGaaaaa > 1:470079/1‑141 (MQ=255) aGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGaaaaa > 1:339607/1‑141 (MQ=255) gATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAaag < 4:30901/141‑1 (MQ=255) aaGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAAGAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGTAAATGATTTAGCAGAAAATGCTGAAGTGAAAaagaag > 4:427034/1‑141 (MQ=255) aaGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAAGAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGTAAATGATTTAGCAGAAAATGCTGAAGTGAAAaagaag > 1:447427/1‑141 (MQ=255) aaGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAaagaag < 1:64085/141‑1 (MQ=255) gTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGcc > 1:152161/1‑141 (MQ=255) tttGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCa > 1:28576/1‑141 (MQ=255) tGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGCGAAAAAGAAGCCACg > 2:670190/1‑141 (MQ=255) gCAACAAATAAAGGAAATGAAGAAGTTAAATGCTTAACAGAAGAACAAGGTAAAGAAAAATTAGAAGATACACATTCTGACGGCTCCACGCAGGATGCAAATGATTTAGCAGAAACTGCTGAAGCGCAAAAGAAGCCACgg > 4:278979/1‑140 (MQ=255) caacaaATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCa > 2:803000/1‑141 (MQ=255) caacaaATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCa > 2:723980/1‑141 (MQ=255) caacaaATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCa > 2:1007214/1‑141 (MQ=255) aaaTAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGtt < 4:384052/141‑1 (MQ=255) aaTAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTg > 4:394908/1‑141 (MQ=255) aTAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGa > 1:620032/1‑141 (MQ=255) tAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACGAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGaaaa > 4:4285/1‑122 (MQ=255) aTGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGACGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCgg > 1:1027991/1‑141 (MQ=255) aTGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCgg > 1:479417/1‑141 (MQ=255) tGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGa > 2:161609/1‑141 (MQ=255) agaagTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGAttt > 3:55953/1‑141 (MQ=255) agaagTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACAATCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGAttt < 2:731156/141‑1 (MQ=255) agTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTtga < 3:90504/141‑1 (MQ=255) tAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTtgatga > 3:230534/1‑141 (MQ=255) aaTCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACg > 1:1042885/1‑141 (MQ=255) ccTTAAGCGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGTAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATg > 1:859345/1‑141 (MQ=255) ccTTAAGAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGTAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATg > 3:373106/1‑141 (MQ=255) ccTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTGCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATg < 2:190379/141‑1 (MQ=255) tAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCt < 1:117502/141‑1 (MQ=255) tAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCt > 1:27715/1‑141 (MQ=255) aaCAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCtt < 3:156088/141‑1 (MQ=255) gaagaaCAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGTAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAata < 3:156480/141‑1 (MQ=255) gaaCAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGA‑TTTGATGACGATGGCTTAATATCaa > 2:337744/1‑141 (MQ=255) aaCAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCaa > 3:369141/1‑141 (MQ=255) aCAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAAt > 3:57978/1‑141 (MQ=255) cAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAAtt > 2:824945/1‑141 (MQ=255) aaGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTg < 1:367081/141‑1 (MQ=255) cAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGAt > 1:1024939/1‑141 (MQ=255) aCAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTATTATCAATTGAAGATTTTg < 2:354340/141‑1 (MQ=255) aCAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTg > 4:37410/1‑141 (MQ=255) aaaTTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTgaa < 2:762880/141‑1 (MQ=255) aaTTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTgaag > 4:188404/1‑141 (MQ=255) aTTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTgaaga > 2:617486/1‑141 (MQ=255) ttAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTgaagaa < 2:949525/141‑1 (MQ=255) tAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGaaa > 3:263567/1‑141 (MQ=255) gaagaTACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAAtt > 2:284942/1‑141 (MQ=255) agaTACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGa > 3:427596/1‑141 (MQ=255) tACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGc < 1:189226/141‑1 (MQ=255) cacaTTCTGAATGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTc < 1:426134/141‑1 (MQ=255) caTTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGTTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCaa > 2:484302/1‑141 (MQ=255) caTTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCaa > 1:59959/1‑141 (MQ=255) caTTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCaa > 1:422166/1‑141 (MQ=255) caTTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCaa > 1:994225/1‑141 (MQ=255) caTTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCaa > 2:650145/1‑141 (MQ=255) tCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCaaaaa > 1:1000499/1‑141 (MQ=255) gAAGGTTCAACGCAGGACGTAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATg > 4:268782/1‑141 (MQ=255) gAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATg > 2:577339/1‑141 (MQ=255) aGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATggg > 4:308848/1‑141 (MQ=255) aGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATggg > 1:244098/1‑141 (MQ=255) gTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATGGGTg < 1:363255/141‑1 (MQ=255) gTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATGGGTg > 3:424451/1‑141 (MQ=255) tCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATGGGTGCt < 3:329534/141‑1 (MQ=255) cAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATGGGTGCTa > 1:856223/1‑141 (MQ=255) cGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATGGGTGCTAAAt > 1:638758/1‑141 (MQ=255) cGCAGCACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCGCAAAAAATGGGTGCTAAAt < 3:278979/141‑1 (MQ=255) aGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATGGGTGCTAAATTTa > 2:598803/1‑141 (MQ=255) ggACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATGGGTGCTAAATTTaa > 2:1027543/1‑141 (MQ=255) gACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATGGGTGCTAAATTTaaa < 1:179291/141‑1 (MQ=255) cGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATGGGTGCTAAATTTAAAGc < 2:795276/141‑1 (MQ=255) gCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATGGGTGCTAAATTTAAAGCa > 2:954163/1‑141 (MQ=255) | AGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAAGCTCAAAAAATGGGTGCTAAATTTAAAGCA > USA300TCH1516_ALE/1250221‑1250500 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |