Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,218,734 A→C 5.1% intergenic (+225/+38) USA300HOU_RS06065 → / ← USA300HOU_RS06070 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,218,7340AC 5.1% 334.0 / 8.6 110intergenic (+225/+38)USA300HOU_RS06065/USA300HOU_RS06070hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (56/47);  new base C (3/3);  total (59/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

TTAGCATTTTAGTTTCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCTGATGGTT  >  USA300TCH1516_ALE/1218594‑1218872
                                                                                                                                            |                                                                                                                                           
ttAGCATTTTAGTTTCACTCATTTTTAAAATCACAAAGATAAGTTGTAAATTAAATGATGATACGTTATTTAAAAACACGGTACTTCGTTTCAATGAACGCATTAAATAATGAATAAACTCCTTACCATATGAAAGGacta                                                                                                                                             <  4:306150/141‑1 (MQ=255)
  aGCATTTTAGTTTCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGactact                                                                                                                                           <  2:475379/141‑1 (MQ=255)
  aGCATTTTAGTTTCACCCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGactact                                                                                                                                           <  1:486890/141‑1 (MQ=255)
    cATTTTAGTTTCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTActtt                                                                                                                                         >  4:59915/1‑141 (MQ=255)
     aTTTTAGTTTCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTActttc                                                                                                                                        <  3:132265/141‑1 (MQ=255)
     aTTTTAGTTTCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTActttc                                                                                                                                        <  2:436891/141‑1 (MQ=255)
     aTTTTAGTTTCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTActttc                                                                                                                                        <  1:467574/141‑1 (MQ=255)
        ttAGTTTCACTCATTTTTAAAATCACAAATATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGAATActttcttt                                                                                                                                    >  4:457665/1‑141 (MQ=255)
        ttAGTTTCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTActttcttt                                                                                                                                    >  4:14754/1‑141 (MQ=255)
         tAGTTTCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCtttt                                                                                                                                   <  4:270968/141‑1 (MQ=255)
         tAGTTTCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCtttt                                                                                                                                   <  1:403078/141‑1 (MQ=255)
          aGTTTCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTtg                                                                                                                                  >  4:134466/1‑141 (MQ=255)
           gTTTCACTCGTTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTtgt                                                                                                                                 >  1:957632/1‑141 (MQ=255)
            tttCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACAAAAGAAAGTAGTCctttcttatggtg                                                                                                                                <  1:950053/141‑7 (MQ=37)
               cACTCATTTTTAAAATCACAAATAAAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTGGTAATTAACGCGTTAAATAAGAAATAGACGCCTCACCATGCGGATTGACTTCTTTCTTTtgctttg                                                                                                                             <  2:331144/141‑6 (MQ=255)
               cACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGTAGTCCTTTCTTAtggtgag                                                                                                                             >  2:512678/1‑136 (MQ=38)
               cACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAgg                                                                                                                             >  4:132042/1‑141 (MQ=255)
                  tCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGgtgt                                                                                                                          <  2:263923/141‑1 (MQ=255)
                      ttttAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGTAGTCCTTTCTTATGGTGAGGTGTTTa                                                                                                                       <  1:512678/141‑1 (MQ=38)
                       tttAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTAtt                                                                                                                     <  1:1082121/141‑1 (MQ=255)
                        ttAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTAttt                                                                                                                    <  2:966766/141‑1 (MQ=255)
                        ttAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTAttt                                                                                                                    <  1:690849/141‑1 (MQ=255)
                         tAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTg                                                                                                                   <  4:335197/141‑1 (MQ=255)
                         tAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTg                                                                                                                   >  2:898991/1‑141 (MQ=255)
                         tAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTg                                                                                                                   >  1:417153/1‑141 (MQ=255)
                           aaaTCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACCCGATACTTCGTTTCAATGAACGCCTTCAATAATAAATAAACACCTCACCATAAGCAAGGACTACTTTCCTTTGTGAGGTGCTTCTTTGTc                                                                                                                 >  1:490520/1‑141 (MQ=255)
                             aTCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGa                                                                                                               >  1:191457/1‑141 (MQ=255)
                             aTCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGa                                                                                                               >  1:787930/1‑141 (MQ=255)
                               tttttaGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAGCACGATACTTCGTTTCAATGCACGCATTATTTAATATATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTTTTTGTCGACa                                                                                                             >  2:840904/6‑141 (MQ=255)
                                 caAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAAc                                                                                                           >  1:12854/1‑141 (MQ=255)
                                     gATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGa                                                                                                       >  3:16950/1‑141 (MQ=255)
                                      aTAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGAt                                                                                                      >  1:299360/1‑141 (MQ=255)
                                       tAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGAtt                                                                                                     >  2:605128/1‑141 (MQ=255)
                                        aaTTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTa                                                                                                    >  2:699643/1‑141 (MQ=255)
                                         aTTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTAt                                                                                                   <  1:497336/141‑1 (MQ=255)
                                                aCTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGtt                                                                                            <  1:1052210/141‑1 (MQ=255)
                                                  ttAAATGATGATACGTTATTTAAAAACAAGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACCTTCTTTTGTGAGGTGTTTACTTGTCGACAACTTGATTATGATCGTATc                                                                                          <  4:149490/141‑1 (MQ=255)
                                                    aaaTGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCAt                                                                                        >  2:172847/1‑141 (MQ=255)
                                                           gatACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGa                                                                                 >  3:89806/1‑141 (MQ=255)
                                                           gatACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGa                                                                                 >  1:7134/1‑141 (MQ=255)
                                                                  tATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGCGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGtt                                                                          <  1:549178/141‑1 (MQ=255)
                                                                    tttAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACCACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCa                                                                        <  1:345013/141‑1 (MQ=255)
                                                                    tttAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCa                                                                        <  1:345001/141‑1 (MQ=255)
                                                                        aaaaCACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAAt                                                                    <  4:207614/141‑1 (MQ=255)
                                                                         aaaCACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATc                                                                   >  2:247612/1‑141 (MQ=255)
                                                                         aaaCACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATc                                                                   <  2:498664/141‑1 (MQ=255)
                                                                           acacGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAg                                                                 <  2:983649/141‑1 (MQ=255)
                                                                           acacGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAg                                                                 >  3:300908/1‑141 (MQ=255)
                                                                            cacGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCGCAAAAGAAAGTAGTCCTTTCTTATGGTGAGGTGTTTATTTGTCGACAACTTGATTATAATTGTTTCATTTTTTGAATAAGTTCATAATCAgg                                                                <  2:296676/141‑1 (MQ=25)
                                                                              cGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGACAGGACTCCTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATGGTTTCATTTTTTGAATAAGTTCATACTCAgtcg                                                              >  2:752447/1‑138 (MQ=255)
                                                                              cGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGgtg                                                              >  1:1020274/1‑141 (MQ=255)
                                                                                 tACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAg                                                           <  1:11803/141‑1 (MQ=255)
                                                                                 tACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAg                                                           >  1:972385/1‑141 (MQ=255)
                                                                                 tACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAg                                                           <  4:452262/141‑1 (MQ=255)
                                                                                   cTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCa                                                         >  4:64577/1‑141 (MQ=255)
                                                                                     tCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATa                                                       >  2:220865/1‑141 (MQ=255)
                                                                                      cGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATAc                                                      <  3:64577/141‑1 (MQ=255)
                                                                                      cGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATAc                                                      <  1:925660/141‑1 (MQ=255)
                                                                                      cGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATAc                                                      <  2:364981/141‑1 (MQ=255)
                                                                                      cGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATAc                                                      <  4:153835/141‑1 (MQ=255)
                                                                                        tttCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACaa                                                    >  4:253721/1‑141 (MQ=255)
                                                                                           cAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGt                                                 <  4:211827/141‑1 (MQ=255)
                                                                                            aaTGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGtt                                                <  1:25775/141‑1 (MQ=255)
                                                                                             aTGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGttt                                               >  2:165322/1‑141 (MQ=255)
                                                                                                 aCGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTg                                           >  1:797330/1‑141 (MQ=255)
                                                                                                 aCGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTg                                           >  4:39696/1‑141 (MQ=255)
                                                                                                   gCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGAt                                         <  3:159747/141‑1 (MQ=255)
                                                                                                    cATTAAATAATAAATAACCACCTCACCATAAGAAAGAACTACTTTCTTTGGTGAGGTGTTTATTTGTCGACAACTTGATTATGAGTGTTTCATTTTTTGAATAAGTTCATAATCAGGTTTAGCATACAAAGTTTTTTGAtt                                        <  2:535074/141‑1 (MQ=255)
                                                                                                    cATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGAtt                                        >  3:36364/1‑141 (MQ=255)
                                                                                                    cATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGAtt                                        >  3:36358/1‑141 (MQ=255)
                                                                                                    cATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGAtt                                        <  1:1020932/141‑1 (MQ=255)
                                                                                                    cATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGAtt                                        <  1:1078256/141‑1 (MQ=255)
                                                                                                     aTTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTTAATAATTTTATAATCAGGTGTAGCATACAAAGTTTTTTGATTg                                       >  2:584137/1‑141 (MQ=255)
                                                                                                     aTTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTg                                       >  1:345057/1‑141 (MQ=255)
                                                                                                      ttAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGt                                      <  1:359457/141‑1 (MQ=255)
                                                                                                        aaataataaataaACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCa                                    >  1:433713/1‑141 (MQ=255)
                                                                                                           cgacaaataaaCACCTCACAAAAGAAAGTAGTCCTTTCTTATGGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGTTTGTCatat                                 >  4:441493/5‑141 (MQ=21)
                                                                                                            aataaataaACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATg                                >  1:569662/1‑141 (MQ=255)
                                                                                                                 ataaaCACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACa                           >  2:582626/1‑141 (MQ=255)
                                                                                                                  taaaCACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACaa                          >  1:37217/1‑141 (MQ=255)
                                                                                                                       acCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTTAATATTTTCATAATCATGTGTAGCATACAAAGTTTTTTGATTTTTATATGTTACAAACCCa                     >  2:978113/1‑141 (MQ=255)
                                                                                                                       acCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCa                     >  2:565242/1‑141 (MQ=255)
                                                                                                                        ccTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAg                    <  3:201457/141‑1 (MQ=255)
                                                                                                                         cTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTTTATTTTTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAgg                   >  2:387159/1‑141 (MQ=255)
                                                                                                                            aCCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCtt                >  3:332281/1‑141 (MQ=255)
                                                                                                                              cATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTg              <  2:149359/141‑1 (MQ=255)
                                                                                                                                  aGATAGGACTACATTCTTTTGTGATTTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCAcc          <  1:97993/141‑1 (MQ=255)
                                                                                                                                  aGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCAcc          <  2:1040425/141‑1 (MQ=255)
                                                                                                                                  aGAAAGGACTACTGTCTTGTGGGTGGGGTTTATTTGTCGACAACTTGATTATGATTGTTTTATTTTTTTAATAAGTTCATAATCAGGTGTAGCAGACAAAGTTTTTTGATTGTCATATGTGACAAACCCAGGCATTGCAcc          >  4:271442/1‑141 (MQ=255)
                                                                                                                                   gAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCt         <  1:827470/141‑1 (MQ=255)
                                                                                                                                   gAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCt         <  1:510008/141‑1 (MQ=255)
                                                                                                                                   gAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCt         <  2:933193/141‑1 (MQ=255)
                                                                                                                                    aaaGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCTg        <  1:743703/141‑1 (MQ=255)
                                                                                                                                    aaaGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCTg        >  2:238366/1‑141 (MQ=255)
                                                                                                                                    aaaGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCTg        >  2:867119/1‑141 (MQ=255)
                                                                                                                                     aaGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCTGa       <  1:749704/141‑1 (MQ=255)
                                                                                                                                     aaGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCAAACAAAGTTTTTTGATTTTCATATGTTACAAACCCAGGCTTTGCACCTGa       >  1:541790/1‑141 (MQ=255)
                                                                                                                                       ggACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCTGATg     >  3:373356/1‑141 (MQ=255)
                                                                                                                                        gACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCTGATgg    <  4:174414/141‑1 (MQ=255)
                                                                                                                                          ctactTTCTTTTGTGGGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCTGATGGtt  <  2:759257/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TTAGCATTTTAGTTTCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCTGATGGTT  >  USA300TCH1516_ALE/1218594‑1218872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: