BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,199,472	G→A	5.3%	Q39Q (CAG→CAA)	USA300HOU_RS05985 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,199,472	0	G	A	5.3%	345.7 / 5.0	110	Q39Q (CAG→CAA)	USA300HOU_RS05985	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (51/53); new base A (4/2); total (55/55)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 112 total aligned reads displayed.

TTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGACCTAATGCTGAAAGAAAAAGAGCAATCATTTCGCC  >  USA300TCH1516_ALE/1199332‑1199612

tttttCTGAATATAAGGGCAATAGATGATATTTTATAATTTTTTTAAGGTAGTGCTTAACATAGATCTTTACCAAGACTCCATCTACGAACAATACGAATTGATTAATCACCTCACGGCTAAACGTGATCACGCGGAACAg                                                                                                                                              <  4:109915/141‑1 (MQ=255)

 ttttCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGc                                                                                                                                             <  4:410218/141‑1 (MQ=255)

 ttttCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGc                                                                                                                                             <  2:433792/141‑1 (MQ=255)

  tttCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCa                                                                                                                                            >  1:164676/1‑141 (MQ=255)

    tCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCAtt                                                                                                                                          >  1:941744/1‑141 (MQ=255)

    tCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAACAtt                                                                                                                                          >  3:112605/1‑141 (MQ=255)

     cTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAGATGTGATCAAGCGGAACAGCATTa                                                                                                                                         >  1:756252/1‑141 (MQ=255)

     cTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTa                                                                                                                                         <  4:160747/141‑1 (MQ=255)

      tGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTAt                                                                                                                                        >  1:1072217/1‑141 (MQ=255)

          tataAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCAc                                                                                                                                    <  2:19737/141‑1 (MQ=255)

          tataAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAAAACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCAc                                                                                                                                    <  4:434381/141‑1 (MQ=255)

          tataAGGGAAATAGATGGTATTTTATAATTTTTTTAAAGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCAc                                                                                                                                    <  2:213945/141‑1 (MQ=255)

           ataAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCAcc                                                                                                                                   <  1:1044987/141‑1 (MQ=255)

             aaGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAg                                                                                                                                 >  1:187239/1‑141 (MQ=255)

                ggCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTAt                                                                                                                              >  1:1022010/1‑141 (MQ=255)

                 gCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTAtt                                                                                                                             >  1:927407/1‑141 (MQ=255)

                   aaTAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTaa                                                                                                                           <  1:1003237/141‑1 (MQ=255)

                   aaTAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTaa                                                                                                                           <  1:905904/141‑1 (MQ=255)

                    aTAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGGTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAAc                                                                                                                          <  2:78858/141‑1 (MQ=255)

                     tAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACg                                                                                                                         <  2:371297/141‑1 (MQ=255)

                     tAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACg                                                                                                                         <  1:55956/141‑1 (MQ=255)

                        agggTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCAt                                                                                                                      >  2:482461/3‑141 (MQ=255)

                        aTGGTATTTTATAATTTTTTTATGGTTGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCAt                                                                                                                      >  2:959798/1‑141 (MQ=255)

                        aTGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCAt                                                                                                                      <  2:693485/141‑1 (MQ=255)

                         tGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCAtt                                                                                                                     >  2:844793/1‑141 (MQ=255)

                         tGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCAtt                                                                                                                     <  1:300516/141‑1 (MQ=255)

                          ggTATTTTATAATTTTTTTTTTGTTGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCAttt                                                                                                                    >  2:296728/1‑141 (MQ=255)

                          ggTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCAttt                                                                                                                    >  1:317753/1‑141 (MQ=255)

                                ttATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGa                                                                                                              >  3:85568/1‑141 (MQ=255)

                                  ataATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGAtt                                                                                                            >  4:387814/1‑140 (MQ=255)

                                  ataATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATc                                                                                                            <  1:1019459/141‑1 (MQ=255)

                                    aaTTTTTTTAAAGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTATCGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCa                                                                                                          >  2:571881/1‑141 (MQ=255)

                                       ttttttAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAaga                                                                                                       >  3:253960/1‑141 (MQ=255)

                                          tttAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCaacatgg                                                                                                    >  4:17709/1‑136 (MQ=255)

                                           ttAAGGTAGTGATTAACATTGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGc                                                                                                   <  3:302964/141‑1 (MQ=255)

                                             aaGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCaa                                                                                                 >  2:374/1‑141 (MQ=255)

                                             aaGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAACATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCaa                                                                                                 <  1:1076438/141‑1 (MQ=255)

                                              aGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAat                                                                                                >  1:106059/1‑141 (MQ=255)

                                              aGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAat                                                                                                <  3:240592/141‑1 (MQ=255)

                                               ggTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAata                                                                                               <  1:353028/141‑1 (MQ=255)

                                                      aTTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTg                                                                                        <  2:449311/141‑1 (MQ=255)

                                                       ttAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTgg                                                                                       <  4:357456/141‑1 (MQ=255)

                                                       ttAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTgg                                                                                       <  4:187924/141‑1 (MQ=255)

                                                       ttAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTgg                                                                                       <  4:357464/141‑1 (MQ=255)

                                                       ttAACATAGATATTTAGCAACACATTAGACAGGAAGAATAAGAATTAATGGATCAGCTAACGGATAAATGTGATAAAGCGGAACAGCATTATGAACCAGTATTAACGAACTTTTTAGGTCCAAGTGGGCAATAGATATTgg                                                                                       <  4:350364/141‑1 (MQ=255)

                                                         aaCATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGaa                                                                                     <  4:124389/141‑1 (MQ=255)

                                                          aCATAGATATTTATTAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGTAg                                                                                    <  4:209982/141‑1 (MQ=255)

                                                          aCATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAg                                                                                    <  1:128863/141‑1 (MQ=255)

                                                          aCATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAg                                                                                    >  2:421767/1‑141 (MQ=255)

                                                           cATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGt                                                                                   >  2:177743/1‑141 (MQ=255)

                                                           cATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGt                                                                                   >  1:998217/1‑141 (MQ=255)

                                                               gATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGGGAtt                                                                               >  3:106673/1‑141 (MQ=255)

                                                                  atTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTtgt                                                                            <  1:849856/141‑1 (MQ=255)

                                                                    ttATCAACGCTTTAGACAGGAAGAATACGCATTAATTGATCAGCTAACGGAAAAATGTGAACACGCGCAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTgg                                                                          >  4:318740/1‑141 (MQ=255)

                                                                    ttATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGAAAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCACTATATATTGGAAGTGATTTGTgg                                                                          >  4:318780/1‑141 (MQ=255)

                                                                     tATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGc                                                                         >  1:971597/1‑141 (MQ=255)

                                                                     tATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGc                                                                         <  4:330811/141‑1 (MQ=255)

                                                                     tATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGc                                                                         >  2:184379/1‑141 (MQ=255)

                                                                     tATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGc                                                                         >  1:16956/1‑141 (MQ=255)

                                                                       tCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAg                                                                       <  1:651985/141‑1 (MQ=255)

                                                                        cAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGt                                                                      <  1:637181/141‑1 (MQ=255)

                                                                           cacTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTAt                                                                   >  3:214709/1‑141 (MQ=255)

                                                                            acTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATg                                                                  <  2:300143/141‑1 (MQ=255)

                                                                            acTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATg                                                                  <  2:148534/141‑1 (MQ=255)

                                                                            acTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATg                                                                  >  2:759778/1‑141 (MQ=255)

                                                                              gtgaGACAGGAAGAAGACGAATTGAGTGATCAGCTAACGGAGGAATGGGATCAAGCGGAACGACAGGAGGCACCAGTATTAACGCACTTTTTAGATCGAAGAGGGCAATATATGCTGGAAGTGATTTGTGGCAGTTTTggg                                                                <  2:1062382/138‑3 (MQ=255)

                                                                                tAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGCGATTTGTGGCAGTTATGAAGa                                                              >  1:143729/1‑141 (MQ=255)

                                                                                  gACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGAtt                                                            >  1:173610/1‑141 (MQ=255)

                                                                                   aCAGGAAGAATACGAATTAATTGATCAGCTAAAGGATAAATGTGCTCAAGCGGAACAACATTATGCACAAGTAATAACGCATTTTTTAGATCCAAGAGGGAAATATATATTGGATGTAATATGTGATAGTTATGAAGAttt                                                           >  4:109043/1‑141 (MQ=255)

                                                                                     aGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTaa                                                         >  3:224771/1‑141 (MQ=255)

                                                                                       gaagaaTACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAAc                                                       >  2:115889/1‑141 (MQ=255)

                                                                                          gaaTACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCACGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTACACGTa                                                    <  2:270563/141‑1 (MQ=255)

                                                                                           aaTACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTAt                                                   <  2:119884/141‑1 (MQ=255)

                                                                                            aTACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATc                                                  <  2:908563/141‑1 (MQ=255)

                                                                                              aCGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCtt                                                >  2:708499/1‑141 (MQ=255)

                                                                                              aCGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCtt                                                >  3:129512/1‑141 (MQ=255)

                                                                                               cGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCttt                                               <  4:249239/141‑1 (MQ=255)

                                                                                                     aattGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTAtgg                                         <  1:92379/141‑1 (MQ=255)

                                                                                                     aagtGATCAGCTAACGGATAAAGGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTAtgg                                         <  1:789349/138‑1 (MQ=255)

                                                                                                        tGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTAtggtgg                                      <  2:536326/141‑1 (MQ=255)

                                                                                                         gATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGa                                     >  1:64740/1‑141 (MQ=255)

                                                                                                           tCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGAcc                                   <  4:102557/141‑1 (MQ=255)

                                                                                                            cAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGACCt                                  <  4:413182/141‑1 (MQ=255)

                                                                                                                    ggATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGACCTAATGCTga                          >  1:1019095/1‑141 (MQ=255)

                                                                                                                    ggATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGACCTAATGCTga                          >  1:672243/1‑141 (MQ=255)

                                                                                                                    ggATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGACCTAATGCTga                          >  2:324279/1‑141 (MQ=255)

                                                                                                                     gATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGACCTAATGCTgaa                         <  3:442416/141‑1 (MQ=255)

                                                                                                                     gATAAATGTGATCAAGCGGAACAGCATTATGAACCAGTATTAACGCATTTTTTAGATGCAAGAGGGCAATATGTAGTGGAAGTGATTTGTGGCAGTTATGAAGATTTAATCGTATCTTTTTATGGTGGTTCTAATGCTgaa                         <  2:54507/141‑1 (MQ=255)

                                                                                                                      aaaaaTGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGACCTAATGCTgaaa                        >  2:1061903/3‑141 (MQ=255)

                                                                                                                      aTAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGACCTAATGCTgaaa                        <  1:950698/141‑1 (MQ=255)

                                                                                                                      aTAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGACCTAATGCTgaaa                        <  1:63414/141‑1 (MQ=255)

                                                                                                                      aTAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGACCTAATGCTgaaa                        <  4:375749/141‑1 (MQ=255)

                                                                                                                        aaaTGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGACCTAATGCTgaaaga                      <  1:674896/141‑1 (MQ=255)

                                                                                                                             tgATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGACCTAATGCTGAAAGAAAAag                 >  1:692512/1‑141 (MQ=255)

                                                                                                                                tgaaggagAACAGCAGTATGCAACAGTATTAACGAAATTTTTTGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGACTGATTTGTATCTTTTTAAGTGCGACATTATGCTGAAAGATAAAGAGc              <  2:923611/134‑1 (MQ=255)

                                                                                                                                    gCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGACCTAATGCTGAAAGAAAAAGAGCAATc          >  2:742504/1‑141 (MQ=255)

                                                                                                                                         aCAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGACCTAATGCTGAAAGAAAAAGAGCAATCATTTc     >  4:131124/1‑141 (MQ=255)

                                                                                                                                         aCAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGACCTAATGCTGAAAGAAAAAGAGCAATCATTTc     >  1:908723/1‑141 (MQ=255)

                                                                                                                                           aGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGACCTAATGCTGAAAGAAAAAGAGCAATCATTTCGc   >  2:173399/1‑141 (MQ=255)

                                                                                                                                            gCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGACCTAATGCTGAAAGAAAAAGAGCAATCATTTCGcc  >  4:302088/1‑141 (MQ=255)

TTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATACGAATTAATTGATCAGCTAACGGATAAATGTGATCAAGCGGAACAGCATTATGCACCAGTATTAACGCATTTTTTAGATCCAAGAGGGCAATATATATTGGAAGTGATTTGTGGCAGTTATGAAGATTTAAACGTATCTTTTTATGGTGGACCTAATGCTGAAAGAAAAAGAGCAATCATTTCGCC  >  USA300TCH1516_ALE/1199332‑1199612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`