New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 2527385 =122 (0.980)3 (0.030) 3/236 NT 2.9% intergenic (‑134/+83) nirC/USA300TCH1516_02411 Nitrite transporter NirC/hypothetical protein
?USA300TCH1516_ALE 2527456 = 87 (0.760)intergenic (‑205/+12) nirC/USA300TCH1516_02411 Nitrite transporter NirC/hypothetical protein
Rejected: Frequency below/above cutoff threshold.

TGCAATTGGTTACAGTGTATTTGCATATGGTTTACATTACTTTTCAAATTTAAAAAAGAATAAGTAATACTTTACATAAAGCGAGATAAAATGAGCGTCGTAATATCTTAAATCATAAAGATATTAGCCAATCACGCGATTTATCTCGCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  USA300TCH1516_ALE/2527534‑2527385
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tttatctcgctTTATGTAAAGTATTACTTATTCTTTTTTAAATTTGAAAAGTAATGTAAACCATATGCAAATACACTGTAACCAATTGCAGTACGTAAAATAAACCTTATAACTTTATTAGGTACACATTTTGACGTTACTGAAATAG  >  USA300TCH1516_ALE/2527456‑2527592
                                                                                                                                                                                                                                                                                               
TGCAATTGGTTACAGTGTATTTGCATATGGTTTACATTACTTTTCAAATTTAAAAAAGAATAAGTAATACTTTACATAAAGCGAGATAAAATGAGCGTCGTAATATCTTAAATCATAAAGATATTAGCCAATCACGCGATT                                                                                                                                                    <  3:394769/141‑1
 GCAATTGGTTACAGTGTATTTGCATATGGTTTACATTACTTTTCAAATTTAAAAAAGAATAAGTAATACTTTACATAAAGCGAGATAAAATGAGCGTCGTAATATCTTAAATCATAAAGATATTAGCCAATCACGCGATTT                                                                                                                                                   >  4:164570/1‑141
  CAATTGGTTACAGTGTATTTGCATATGGTTTACATTACTTTTCAAATTTAAAAAAGAATAAGTAATACTTTACATAAAGCGAGATAAAATGAGCGTCGTAATATCTTAAATCATAAAGATATTAGCCAATCACGCGATTTA                                                                                                                                                  <  4:86557/141‑1
  CAATTGGTTACAGTGTATTTGCATATGGTTTACATTACTTTTCAAATTTAAAAAAGAATAAGTAATACTTTACATAAAGCGAGATAAAATGAGCGTCGTAATATCTTAAATCATAAAGATATTAGCCAATCACGCGATTTA                                                                                                                                                  <  2:461901/141‑1
     TTGGTTACAGTGTATTTGCATATGGTTTACATTACTTTTCAAATTTAAAAAAGAATAAGTAATACTTTACATAAAGCGAGATAAAATGAGCGTCGTAATATCTTAAATCATAAAGATATTAGCCAATCACGCGATTTATCT                                                                                                                                               >  1:916890/1‑141
      TGGTTACAGTGTATTTGCATATGGTTTACATTACTTTTCAAATTTAAAAAAGAATAAGTAATACTTTACATAAAGCGAGATAAAATGAGCGTCGTAATATCTTAAATCATAAAGATATTAGCCAATCACGCGATTTATCTC                                                                                                                                              >  2:1057486/1‑141
       GGTTACAGTGTATTTGCATATGGTTTACATTACTTTTCAAATTTAAAAAAGAATAAGTAATACTTTACATAAAGCGAGATAAAATGAGCGTCGTAATATCTTAAATCATAAAGATATTAGCCAATCACGCGATTTATCTCG                                                                                                                                             <  1:452064/141‑1
       GGTTACAGTGTATTTGCATATGGTTTACATTACTTTTCAAATTTAAAAAAGAATAAGTAATACTTTACATAAAGCGAGATAAAATGAGCGTCGTAATATCTTAAATCATAAAGATATTAGCCAATCACGCGATTTATCTCG                                                                                                                                             >  1:468523/1‑141
       GGTTACAGTGTATTTGCATATGGTTTACATTACTTTTCAAATTTAAAAAAGAATAAGTAATACTTTACATAAAGCGAGATAAAATGAGCGTCGTAATATCTTAAATCATAAAGATATTAGCCAATCACGCGATTTATCTCG                                                                                                                                             >  3:257532/1‑141
        GTTACAGTGTATTTGCATATGGTTTACATTACTTTTCAAATTTAAAAAAGAATAAGTAATACTTTACATAAAGCGAGATAAAATGAGCGTCGTAATATCTTAAATCATAAAGATATTAGCCAATCACGCGATTTATCTCGC                                                                                                                                            <  1:324668/141‑1
        GTTACAGTGTATTTGCATATGGTTTACATTACTTTTCAAATTTAAAAAAGAATAAGTAATACTTTACATAAAGCGAGATAAAATGAGCGTCGTAATATCTTAAATCATAAAGATATTAGCCAATCACGCGATTTATCTCGC                                                                                                                                            <  3:222060/141‑1
         TTACAGTGTATTTGCATATGGTTTACATTACTTTTCAAATTTAAAAAAGAATAAGTAATACTTTACATAAAGCGAGATAAAATGAGAGTCGTAATATCTTAAATCATAAAGATATTAGCCAATCACGCGATTTATCTCGCT                                                                                                                                           >  2:862540/1‑141
                                                                   TACTTTACATAAAGCGAGATAAAATGAGCGTCGTAATATCTTAAATCATAAAGATATTAGCCAATCACGCGATTTATCTCGCTTTATGTAAAGTATTACTTATTCTTTTTTAAATTTGAAAAGTAATGTAAACCATATGCA                                                                                 >  1:536515/1‑141
                                                                            TAAAGCGAGATAAAATGAGCGTCGTAATATCTTAAATCATAAAGATATTAGCCAATCACGCGATTTATCTCGCTTTATGTAAAGTATTACTTATTCTTTTTTAAATTTGAAAAGTAATGTAAACCATATGCAAATACACTG                                                                        >  3:163143/1‑141
                                                                                    GATAAAATGAGCGTCGTAATATCTTAAATCATAAAGATATTAGCCAATCACGCGATTTATCTCGCTTTATGTAAAGTATTACTTATTCTTTTTTAAATTTGAAAAGTAATGTAAACCATATGCAAATACACTGTAACCAAT                                                                >  2:539257/1‑141
                                                                                                                                           TTTATCTCGCTTTATGTAAAGTATTACTTATTCTTTTTTAAATTTGAAAAGTAATGTAAACCATATGCAAATACACTGTAACCAATTGCAGTACGTAAAATAAACCTTATAACTTTATTAGGTACACATTTTGACGTTACT         <  3:259340/141‑1
                                                                                                                                           TTTATCTCGCTTTATGTAAAGTATTACTTATTCTTTTTTAAATTTGAAAAGTAATGTAAACCATATGCAAATACACTGTAACCAATTGCAGTACGTAAAATAAACCTTATAACTTTATTAGGTACACATTTTGACGTTACT         >  1:262161/1‑141
                                                                                                                                           TTTATCTCGCTTTATGTAAAGTATTACTTATTCTTTTTTAAATTTGAAAAGTAATGTAAACCATATGCAAATACACTGTAACCAATTGCAGTACGTAAAATAAACCTTATAACTTTATTAGGTACACATTTTGACGTTACT         <  2:602301/141‑1
                                                                                                                                           TTTATCTCGCTTTATGTAAAGTATTACTTATTCTTTTTTAAATTTGAAAAGTAATGTAAACCATATGCAAATACACTGTAACCAATTGCAGTACGTAAAATAAACCTTATAACTTTATTAGGTACACATTTTGACGTTACT         >  1:262198/1‑141
                                                                                                                                           TTTATCTCGCTTTATGTAAAGTATTACTTATTCTTTTTTAAATTTGAAAAGTAATGTAAACCATATGCAAATACACTGTAACCAATTGCAGTACGTAAAATAAACCTTATAACTTTATTAGGTACACATTTTGACGATACT         >  1:192024/1‑141
                                                                                                                                             TATCTCGCTTTATGTAAAGTATTACTTATTCTTTTTTAAGTTTGAAAAGTAATGTAAACCATATGCAAATACACTGTAACCAATTGCAGTACGTAAAATAAACCTTATAACTTTATTAGGTACACATTTTGACGTTACTGA       >  1:652707/1‑141
                                                                                                                                               TCTCGCTTTATGTAAAGTATTACTTATTCTTTTTTAAATTTGAAAAGTAATGTAAACCATATGCAAATACACTGTAACCAATTGCAGTACGTAAAATGAACCTTATAACTTTATTAGGTACACATTTTGACGTTACTGAAA     >  1:854825/1‑141
                                                                                                                                               TCTCGCTTTATGTAAAGTATTACTTATTCTTTTTTAAATTTGAAAAGTAATGTAAACCATATGCAAATACACTGTAACCAATTGCAGTACGTAAAATAAACCTTATAACTTTATTAGGTACACATTTTGACGTTACTGAAA     <  2:1048253/141‑1
                                                                                                                                               TCTCGCTTTATGTAAAGTATTACTTATTCTTTTTTAAATTTGAAAAGTAATGTAAACCATATGCAAATACACTGTAACCAATTGCAGTACGTAAAATAAACCTTATAACTTTATTAGGTACACATTTTGACGTTACTGAAA     <  4:363982/141‑1
                                                                                                                                                tttttTTTATGTAAAGTATTACTTATTCTTTTTTAAATTTGAAAAGTAATGTAAACCATATGCAAATACACTGTAACCCATTGCAGTACGTAAAATAAACCTTATAACTTTATTAGGTACACACTTTGACGTTACTGAAAT    >  2:317148/6‑141
                                                                                                                                                tTCGCTTTATGTAAAGTATTACTTATTCTTTTTTAAATTTGAAAAGTAATGTAAACCATATGCAAATACACTGTAACCAATTGCAGTACGTAAAATAAACCTTATAACTTTATTAGGTACACATTTTGACGTTACTGAAAT    <  4:274666/140‑1
                                                                                                                                                CTCGCTTTATGTAAAGTATTACTTATTCTTTTTTAAATTTGAAAAGTAATGTAAACCATATGCAAATACACTGTAACCAATTGCAGTACGTAAAATAAACCTTATAACTTTCTTAGGTACACATTTCTACGTTACTGAAAT    >  4:374077/1‑141
                                                                                                                                                  CGCTTTATGTAAAGTATTACTTATTCTTTTTTAAATTTGAAAAGTAATGTAAACCATATGCAAATACACTGTAACCAATTGCAGTACGTAAAATAAACCTTATAACTTTATTAGGTACACATTTTGACGTTACTGAAATAG  <  1:580923/141‑1
                                                                                                                                                  CGCTTTATGTAAAGTATTACTTATTCTTTTTTAAATTTGAAAAGTAATGTAAACCATATGCAAATACACTGTAACCAATTGCAGTACGTAAAATAAACCTTATAACTTTATTAGGTACACATTTTGACGTTACGGAAATAG  <  1:860003/141‑1
                                                                                                                                                                                                                                                                                               
TGCAATTGGTTACAGTGTATTTGCATATGGTTTACATTACTTTTCAAATTTAAAAAAGAATAAGTAATACTTTACATAAAGCGAGATAAAATGAGCGTCGTAATATCTTAAATCATAAAGATATTAGCCAATCACGCGATTTATCTCGCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  USA300TCH1516_ALE/2527534‑2527385
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tttatctcgctTTATGTAAAGTATTACTTATTCTTTTTTAAATTTGAAAAGTAATGTAAACCATATGCAAATACACTGTAACCAATTGCAGTACGTAAAATAAACCTTATAACTTTATTAGGTACACATTTTGACGTTACTGAAATAG  >  USA300TCH1516_ALE/2527456‑2527592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.