BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	90,249	Δ1 bp	5.8%	coding (858/870 nt)	USA300TCH1516_00080 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	90,249	0	T	.	5.8%	325.2 / 14.2	103	coding (858/870 nt)	USA300TCH1516_00080	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (49/48); new base . (4/2); total (53/50)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

Only 100 of 110 total aligned reads displayed.

AATAAAATAGAAGTATTAGATACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAATGATGTCAGAATTGAAGTG  >  USA300TCH1516_ALE/90109‑90389

aaTAAAATAGATGTATTAGTTACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATGTTTAGAACTAAGTAATATGATACTCGTGTTAGATGATATGCTCGACGTCAat                                                                                                                                              <  1:509298/141‑1 (MQ=255)

 aTAAAATAGAAGTATTAGATACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAata                                                                                                                                             >  2:963235/1‑141 (MQ=255)

     aaTAGAAGTATTAGATACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTNTTAGATGATATGCTCGACGTCAatatata                                                                                                                                         <  1:730766/141‑1 (MQ=255)

      aTAGAAGTATTAGATACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAatatatat                                                                                                                                        >  1:89070/1‑141 (MQ=255)

         gAAGTATTAGATACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAatatatatata                                                                                                                                     >  1:1022016/1‑141 (MQ=255)

         gAAGTATTAGATACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAatatatatata                                                                                                                                     <  3:143274/141‑1 (MQ=255)

                  aaTACTTTATTGCTAAAATAACTATCGCATTCTTCAACTACTGAAAATATATCTTTAGATATATCAAGATTTTTACAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCtt                                                                                                                            <  2:1057845/140‑1 (MQ=255)

                   aTACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCttt                                                                                                                           >  2:189458/1‑141 (MQ=255)

                     aCTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTa                                                                                                                         <  1:1056710/141‑1 (MQ=255)

                      cTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCA‑‑ATATATATATAAATTCTTTTaaaa                                                                                                                      >  1:96225/1‑141 (MQ=255)

                        ttATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTaaaa                                                                                                                      >  1:387671/1‑141 (MQ=255)

                          aTTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAaat                                                                                                                    <  1:167352/141‑1 (MQ=255)

                            tGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAaataa                                                                                                                  <  1:871182/141‑1 (MQ=255)

                             gCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAaataat                                                                                                                 >  2:823451/1‑141 (MQ=255)

                              cTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAataata                                                                                                                <  1:375243/141‑1 (MQ=255)

                                aaaaaaaaTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACa                                                                                                              >  3:402638/1‑141 (MQ=255)

                                     caCTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAACTAATCCATTaga                                                                                                         >  2:482289/2‑141 (MQ=255)

                                     aaCTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTaga                                                                                                         >  2:599819/1‑141 (MQ=255)

                                     aaCTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCA‑‑ATATATATATAAATTCTTTTAAAAATAATACATTAgaga                                                                                                       <  1:182354/141‑1 (MQ=255)

                                      aCTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTagag                                                                                                        >  4:181363/1‑141 (MQ=255)

                                      aCTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTagag                                                                                                        <  3:143999/141‑1 (MQ=255)

                                        tATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGAc                                                                                                      >  2:669060/1‑141 (MQ=255)

                                        tATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGAc                                                                                                      >  2:494869/1‑141 (MQ=255)

                                        tATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGAc                                                                                                      >  2:1056556/1‑141 (MQ=255)

                                         aTCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCGATATAAATATATAAATTCTTTTAAAAATAATACATTATAGAcg                                                                                                     >  3:85819/1‑140 (MQ=255)

                                          tCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCACTATATATATATAAATTCTTTTAAAAATAATACATTAGAGACAt                                                                                                    >  1:581450/1‑141 (MQ=255)

                                          tCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACAt                                                                                                    >  2:755390/1‑141 (MQ=255)

                                          tCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACAt                                                                                                    <  2:29544/141‑1 (MQ=255)

                                          tCGAATACTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACAt                                                                                                    <  2:527883/141‑1 (MQ=255)

                                                tcttcAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATg                                                                                              <  1:47802/141‑1 (MQ=255)

                                                 cttcAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGc                                                                                             >  2:360220/1‑141 (MQ=255)

                                                   tcAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGa                                                                                           >  1:665142/1‑141 (MQ=255)

                                                     aaCTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACt                                                                                         >  3:126910/1‑141 (MQ=255)

                                                     aaCTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACt                                                                                         <  1:40339/141‑1 (MQ=255)

                                                      aCTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTg                                                                                        >  2:994102/1‑141 (MQ=255)

                                                      aCTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTg                                                                                        >  1:273867/1‑141 (MQ=255)

                                                       cTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCA‑‑ATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATc                                                                                     >  2:379026/1‑141 (MQ=255)

                                                          cAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATcc                                                                                    <  1:691025/141‑1 (MQ=255)

                                                          cAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATcc                                                                                    <  4:161211/141‑1 (MQ=255)

                                                          cAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAAAACTAAAAAAAATAATACATTAGAGACATTTGATGCGACTGATcc                                                                                    <  4:131023/141‑1 (MQ=255)

                                                           aGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCt                                                                                   >  4:404558/1‑141 (MQ=255)

                                                           aGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCt                                                                                   <  1:1085216/141‑1 (MQ=255)

                                                             aaaaTATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGa                                                                                 <  1:679353/141‑1 (MQ=255)

                                                                atatatCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGt                                                                              <  2:1085988/141‑1 (MQ=255)

                                                                   tatCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGa                                                                           >  1:46197/1‑141 (MQ=255)

                                                                   tatCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGa                                                                           <  3:296566/141‑1 (MQ=255)

                                                                    atCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGAt                                                                          <  1:983921/141‑1 (MQ=255)

                                                                     tCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGATACATTTGATGCGACTGATTCTTAAGTTGAtt                                                                         >  2:616729/1‑141 (MQ=255)

                                                                        ataGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACa                                                                      <  4:399626/141‑1 (MQ=255)

                                                                        ataGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACa                                                                      >  2:223826/1‑141 (MQ=255)

                                                                          aGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATc                                                                    <  2:701879/141‑1 (MQ=255)

                                                                            atatatCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCAc                                                                  <  1:622681/141‑1 (MQ=255)

                                                                            atatatCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCAc                                                                  <  4:30859/141‑1 (MQ=255)

                                                                             tatatCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACt                                                                 <  1:596348/141‑1 (MQ=255)

                                                                             tatatCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACt                                                                 <  1:1046517/141‑1 (MQ=255)

                                                                             tatatCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACt                                                                 <  2:1058534/141‑1 (MQ=255)

                                                                                 tCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGaaa                                                             >  2:266508/1‑141 (MQ=255)

                                                                                  cAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCA‑‑ATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAg                                                          <  2:494446/141‑1 (MQ=255)

                                                                                   aaGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAaataa                                                                                                                  <  2:244926/86‑1 (MQ=255)

                                                                                   aaGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAaataa                                                                                                                  >  1:244926/1‑86 (MQ=255)

                                                                                    aGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAg                                                          <  2:379436/141‑1 (MQ=255)

                                                                                      aTTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGga                                                        <  1:931653/141‑1 (MQ=255)

                                                                                       tttttAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGgag                                                       >  1:961426/1‑141 (MQ=255)

                                                                                       tttttAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGgag                                                       <  3:384465/141‑1 (MQ=255)

                                                                                         tttAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTAAATCAATGACATAGGAGAt                                                     >  2:1024299/1‑141 (MQ=255)

                                                                                           tAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTa                                                   >  4:248260/1‑141 (MQ=255)

                                                                                           tAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTa                                                   >  4:248212/1‑141 (MQ=255)

                                                                                           tAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCA‑‑ATATATATATAAATTCTTTGAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACt                                                 >  3:156800/1‑141 (MQ=255)

                                                                                            agcaCTAAGTAATATGAAACTCTTGTTAGATGATATGCTCGACGCCAATATATATATATAAATTATTTTAAATATAATACATTAGAGACATTTGATGCGACTGCTCCTGAAGTTGATTCCATCACTAAAATAGGAGACTAc                                                  >  2:829538/4‑141 (MQ=255)

                                                                                            aGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGGTTAc                                                  >  1:832496/1‑141 (MQ=255)

                                                                                            aGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTCGATTACATCACTGAAATAGGAGATTAc                                                  >  2:727680/1‑141 (MQ=255)

                                                                                             gAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACt                                                 <  2:903026/141‑1 (MQ=255)

                                                                                              aaCTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGCGATTACtt                                                >  2:361839/1‑141 (MQ=255)

                                                                                              aaCTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACtt                                                >  2:201215/1‑141 (MQ=255)

                                                                                                cTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACttat                                              >  2:38842/1‑141 (MQ=255)

                                                                                                 tAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACttatt                                             >  1:673641/1‑141 (MQ=255)

                                                                                                 tAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACttatt                                             >  3:421269/1‑141 (MQ=255)

                                                                                                   aGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTaa                                           <  1:189458/141‑1 (MQ=255)

                                                                                                   aGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACAGTAGAGACATTTGATGCGACTAATTCTGAAGTTGATTACATCACTGTAATAGGAGATTACTTATTaa                                           >  2:822349/1‑141 (MQ=255)

                                                                                                    gTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTaaa                                          >  1:521263/1‑141 (MQ=255)

                                                                                                       atatGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAata                                       >  2:1065551/1‑141 (MQ=255)

                                                                                                         atGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATg                                     <  1:313279/141‑1 (MQ=255)

                                                                                                          tGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGa                                    <  4:396112/141‑1 (MQ=255)

                                                                                                            aTACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACg                                  <  1:593049/141‑1 (MQ=255)

                                                                                                                tctTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTaa                              >  4:304577/1‑141 (MQ=255)

                                                                                                                 ctTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAAt                             <  1:944355/141‑1 (MQ=255)

                                                                                                                 ctTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAAt                             <  1:1009026/141‑1 (MQ=255)

                                                                                                                    gTTAGATGATATGCTCGACGTCCATCTATATATATAAATTCTTTTAAAAATAATCCCTTCGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGCGATTACTTATTACACTATGACGTTAATTCt                          >  4:318768/1‑141 (MQ=255)

                                                                                                                    gTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCt                          <  4:66808/141‑1 (MQ=255)

                                                                                                                      tAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCtat                        <  1:1056556/141‑1 (MQ=255)

                                                                                                                         atgatATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATaaa                     >  2:956550/1‑141 (MQ=255)

                                                                                                                            atatGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAatga                  <  3:305833/141‑1 (MQ=255)

                                                                                                                                gCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAATGATGTc              <  3:63339/141‑1 (MQ=255)

                                                                                                                                gCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAATGATGTc              >  4:307099/1‑141 (MQ=255)

                                                                                                                                gCTCGACGTCA‑‑ATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAATGATGTCAg            >  2:781790/1‑141 (MQ=255)

                                                                                                                                  tCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGTTGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGAAGATTACTTATTAAAATCTGACGTTAATTCTATAAATGGTGTCAg            >  2:894467/1‑141 (MQ=255)

                                                                                                                                  tCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAatgatg                <  1:515086/137‑1 (MQ=255)

                                                                                                                                  tCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAatgatg                >  2:515086/1‑137 (MQ=255)

                                                                                                                                  tCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAATGATGTCAg            <  2:974700/141‑1 (MQ=255)

                                                                                                                                            tatatatatataAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAATGATGTCAGAATTGAAGTg  >  1:346832/1‑141 (MQ=255)

AATAAAATAGAAGTATTAGATACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAATGATGTCAGAATTGAAGTG  >  USA300TCH1516_ALE/90109‑90389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`