BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	90,248	Δ1 bp	5.8%	coding (857/870 nt)	USA300TCH1516_00080 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	90,248	0	A	.	5.8%	328.0 / 14.3	103	coding (857/870 nt)	USA300TCH1516_00080	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (48/48); new base . (4/2); total (53/50)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

Only 100 of 109 total aligned reads displayed.

AATAAAATAGAAGTATTAGATACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAATGATGTCAGAATT  >  USA300TCH1516_ALE/90109‑90383

aaTAAAATAGATGTATTAGTTACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATGTTTAGAACTAAGTAATATGATACTCGTGTTAGATGATATGCTCGACGTCAat                                                                                                                                        <  1:509298/141‑1 (MQ=255)

 aTAAAATAGAAGTATTAGATACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAata                                                                                                                                       >  2:963235/1‑141 (MQ=255)

     aaTAGAAGTATTAGATACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTNTTAGATGATATGCTCGACGTCAatatata                                                                                                                                   <  1:730766/141‑1 (MQ=255)

      aTAGAAGTATTAGATACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAatatatat                                                                                                                                  >  1:89070/1‑141 (MQ=255)

         gAAGTATTAGATACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAatatatatata                                                                                                                               >  1:1022016/1‑141 (MQ=255)

         gAAGTATTAGATACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAatatatatata                                                                                                                               <  3:143274/141‑1 (MQ=255)

                  aaTACTTTATTGCTAAAATAACTATCGCATTCTTCAACTACTGAAAATATATCTTTAGATATATCAAGATTTTTACAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCtt                                                                                                                      <  2:1057845/140‑1 (MQ=255)

                   aTACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCttt                                                                                                                     >  2:189458/1‑141 (MQ=255)

                     aCTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTa                                                                                                                   <  1:1056710/141‑1 (MQ=255)

                      cTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCA‑‑ATATATATATAAATTCTTTTaaaa                                                                                                                >  1:96225/1‑141 (MQ=255)

                        ttATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTaaaa                                                                                                                >  1:387671/1‑141 (MQ=255)

                          aTTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAaat                                                                                                              <  1:167352/141‑1 (MQ=255)

                            tGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAaataa                                                                                                            <  1:871182/141‑1 (MQ=255)

                             gCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAaataat                                                                                                           >  2:823451/1‑141 (MQ=255)

                              cTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAataata                                                                                                          <  1:375243/141‑1 (MQ=255)

                                aaaaaaaaTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACa                                                                                                        >  3:402638/1‑141 (MQ=255)

                                     caCTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAACTAATCCATTaga                                                                                                   >  2:482289/2‑141 (MQ=255)

                                     aaCTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTaga                                                                                                   >  2:599819/1‑141 (MQ=255)

                                     aaCTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCA‑‑ATATATATATAAATTCTTTTAAAAATAATACATTAgaga                                                                                                 <  1:182354/141‑1 (MQ=255)

                                        tATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGAc                                                                                                >  2:669060/1‑141 (MQ=255)

                                        tATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGAc                                                                                                >  2:1056556/1‑141 (MQ=255)

                                        tATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGAc                                                                                                >  2:494869/1‑141 (MQ=255)

                                        tATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGAc                                                                                                >  1:515594/1‑141 (MQ=255)

                                         aTCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCGATATAAATATATAAATTCTTTTAAAAATAATACATTATAGAcg                                                                                               >  3:85819/1‑140 (MQ=255)

                                          tCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCACTATATATATATAAATTCTTTTAAAAATAATACATTAGAGACAt                                                                                              >  1:581450/1‑141 (MQ=255)

                                          tCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACAt                                                                                              <  2:29544/141‑1 (MQ=255)

                                          tCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACAt                                                                                              >  2:755390/1‑141 (MQ=255)

                                          tCGAATACTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACAt                                                                                              <  2:527883/141‑1 (MQ=255)

                                           cGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACAtt                                                                                             <  1:268253/141‑1 (MQ=255)

                                                tcttcAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATg                                                                                        <  1:47802/141‑1 (MQ=255)

                                                 cttcAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGc                                                                                       >  2:360220/1‑141 (MQ=255)

                                                   tcAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGa                                                                                     >  1:665142/1‑141 (MQ=255)

                                                     aaCTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACt                                                                                   >  3:126910/1‑141 (MQ=255)

                                                     aaCTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACt                                                                                   <  1:40339/141‑1 (MQ=255)

                                                      aCTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTg                                                                                  >  2:994102/1‑141 (MQ=255)

                                                      aCTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTg                                                                                  >  1:273867/1‑141 (MQ=255)

                                                       cTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCA‑‑ATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATc                                                                               >  2:379026/1‑141 (MQ=255)

                                                         aCAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATc                                                                               <  1:805919/141‑1 (MQ=255)

                                                          cAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATcc                                                                              <  4:161211/141‑1 (MQ=255)

                                                          cAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATcc                                                                              <  1:691025/141‑1 (MQ=255)

                                                          cAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAAAACTAAAAAAAATAATACATTAGAGACATTTGATGCGACTGATcc                                                                              <  4:131023/141‑1 (MQ=255)

                                                           aGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCt                                                                             <  1:1085216/141‑1 (MQ=255)

                                                           aGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCt                                                                             >  4:404558/1‑141 (MQ=255)

                                                             aaaaTATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGa                                                                           <  1:679353/141‑1 (MQ=255)

                                                              aaaTATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGaa                                                                          <  2:563858/141‑1 (MQ=255)

                                                                atatatCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGt                                                                        <  2:1085988/141‑1 (MQ=255)

                                                                   tatCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGa                                                                     <  3:296566/141‑1 (MQ=255)

                                                                   tatCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGa                                                                     <  2:852691/141‑1 (MQ=255)

                                                                   tatCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGa                                                                     >  1:46197/1‑141 (MQ=255)

                                                                    atCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGAt                                                                    <  1:983921/141‑1 (MQ=255)

                                                                     tCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGATACATTTGATGCGACTGATTCTTAAGTTGAtt                                                                   >  2:616729/1‑141 (MQ=255)

                                                                        ataGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACa                                                                >  2:223826/1‑141 (MQ=255)

                                                                        ataGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACa                                                                <  4:399626/141‑1 (MQ=255)

                                                                          aGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATc                                                              <  2:701879/141‑1 (MQ=255)

                                                                            atatatCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCAc                                                            <  1:622681/141‑1 (MQ=255)

                                                                            atatatCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCAc                                                            <  4:30859/141‑1 (MQ=255)

                                                                             tatatCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACt                                                           <  1:1046517/141‑1 (MQ=255)

                                                                             tatatCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACt                                                           <  2:1058534/141‑1 (MQ=255)

                                                                                 tCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGaaa                                                       >  2:266508/1‑141 (MQ=255)

                                                                                   aaGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAaataa                                                                                                            <  2:244926/86‑1 (MQ=255)

                                                                                   aaGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAaataa                                                                                                            >  1:244926/1‑86 (MQ=255)

                                                                                    aGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAg                                                    <  2:379436/141‑1 (MQ=255)

                                                                                      aTTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGga                                                  <  1:931653/141‑1 (MQ=255)

                                                                                       tttttAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGgag                                                 <  3:384465/141‑1 (MQ=255)

                                                                                       tttttAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGgag                                                 >  1:961426/1‑141 (MQ=255)

                                                                                         tttAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTAAATCAATGACATAGGAGAt                                               >  2:1024299/1‑141 (MQ=255)

                                                                                           tAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTa                                             >  4:248212/1‑141 (MQ=255)

                                                                                           tAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTa                                             >  4:248260/1‑141 (MQ=255)

                                                                                           tAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCA‑‑ATATATATATAAATTCTTTGAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACt                                           >  3:156800/1‑141 (MQ=255)

                                                                                            agcaCTAAGTAATATGAAACTCTTGTTAGATGATATGCTCGACGCCAATATATATATATAAATTATTTTAAATATAATACATTAGAGACATTTGATGCGACTGCTCCTGAAGTTGATTCCATCACTAAAATAGGAGACTAc                                            >  2:829538/4‑141 (MQ=255)

                                                                                            aGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGGTTAc                                            >  1:832496/1‑141 (MQ=255)

                                                                                            aGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCAATGAAATAGGAGATTAc                                            >  2:315130/1‑141 (MQ=255)

                                                                                            aGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTCGATTACATCACTGAAATAGGAGATTAc                                            >  2:727680/1‑141 (MQ=255)

                                                                                             gAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACt                                           <  2:903026/141‑1 (MQ=255)

                                                                                              aaCTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGCGATTACtt                                          >  2:361839/1‑141 (MQ=255)

                                                                                                cTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACttat                                        >  2:38842/1‑141 (MQ=255)

                                                                                                 tAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACttatt                                       >  1:673641/1‑141 (MQ=255)

                                                                                                 tAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACttatt                                       >  3:421269/1‑141 (MQ=255)

                                                                                                   aGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTaa                                     <  1:189458/141‑1 (MQ=255)

                                                                                                    gTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTaaa                                    >  1:521263/1‑141 (MQ=255)

                                                                                                       atatGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAata                                 >  2:1065551/1‑141 (MQ=255)

                                                                                                          tGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGa                              <  4:396112/141‑1 (MQ=255)

                                                                                                            aTACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACg                            <  1:593049/141‑1 (MQ=255)

                                                                                                                tctTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTaa                        >  4:304577/1‑141 (MQ=255)

                                                                                                                 ctTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAAt                       <  1:1009026/141‑1 (MQ=255)

                                                                                                                    gTTAGATGATATGCTCGACGTCCATCTATATATATAAATTCTTTTAAAAATAATCCCTTCGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGCGATTACTTATTACACTATGACGTTAATTCt                    >  4:318768/1‑141 (MQ=255)

                                                                                                                    gTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCt                    <  4:66808/141‑1 (MQ=255)

                                                                                                                    gTTAGATGATATGCTCGACGTCAATATATATATATAAATGCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCt                    >  4:206311/1‑141 (MQ=255)

                                                                                                                      tAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCtat                  <  1:1056556/141‑1 (MQ=255)

                                                                                                                         atgatATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATaaa               >  2:956550/1‑141 (MQ=255)

                                                                                                                            atatGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAatga            <  3:305833/141‑1 (MQ=255)

                                                                                                                                gCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAATGATGTc        <  3:63339/141‑1 (MQ=255)

                                                                                                                                gCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAATGATGTc        >  4:307099/1‑141 (MQ=255)

                                                                                                                                gCTCGACGTCA‑‑ATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAATGATGTCAg      >  2:781790/1‑141 (MQ=255)

                                                                                                                                  tCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGTTGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGAAGATTACTTATTAAAATCTGACGTTAATTCTATAAATGGTGTCAg      >  2:894467/1‑141 (MQ=255)

                                                                                                                                  tCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAatgatg          <  1:515086/137‑1 (MQ=255)

                                                                                                                                  tCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAatgatg          >  2:515086/1‑137 (MQ=255)

                                                                                                                                  tCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAATGATGTCAg      <  2:974700/141‑1 (MQ=255)

                                                                                                                                   cGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAATGATGTCAGa     <  2:105678/141‑1 (MQ=255)

                                                                                                                                      cGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAATGATGTCAGAAtt  <  3:181363/141‑1 (MQ=255)

AATAAAATAGAAGTATTAGATACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGACGTCAATATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCTATAAATGATGTCAGAATT  >  USA300TCH1516_ALE/90109‑90383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`