mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | PAL24_S24_L007_R1_001.good.fq | 7,032,137 | 638,565,345 | 100.0% | 90.8 bases | 91 bases | 98.8% |
| errors | PAL24_S24_L007_R2_001.good.fq | 7,032,137 | 638,565,345 | 100.0% | 90.8 bases | 91 bases | 97.9% |
| total | 14,064,274 | 1,277,130,690 | 100.0% | 90.8 bases | 91 bases | 98.3% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | USA300TCH1516_ALE | 2,872,915 | 407.7 | 3.9 | 93.5% | Staphylococcus species strain strain. |
| coverage | distribution | CP000731 | 27,041 | 1548.1 | 8.3 | 3.3% | Staphylococcus aureus subsp. aureus USA300_TCH1516 plasmid |
| coverage | distribution | NC_012417 | 3,125 | 13299.7 | 53.3 | 3.2% | Staphylococcus aureus subsp. aureus USA300_TCH1516 plasmid |
| total | 2,903,081 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 100066 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 4 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 1082 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.071 |
| reference sequence | pr(no read start) |
|---|---|
| USA300TCH1516_ALE | 0.22392 |
| CP000731 | 0.03427 |
| NC_012417 | 0.02685 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 02:14:53 08 Oct 2019 | 02:17:45 08 Oct 2019 | 2 minutes 52 seconds |
| Read alignment to reference genome | 02:17:45 08 Oct 2019 | 02:35:44 08 Oct 2019 | 17 minutes 59 seconds |
| Preprocessing alignments for candidate junction identification | 02:35:44 08 Oct 2019 | 02:38:14 08 Oct 2019 | 2 minutes 30 seconds |
| Preliminary analysis of coverage distribution | 02:38:14 08 Oct 2019 | 02:42:49 08 Oct 2019 | 4 minutes 35 seconds |
| Identifying junction candidates | 02:42:49 08 Oct 2019 | 02:44:43 08 Oct 2019 | 1 minute 54 seconds |
| Re-alignment to junction candidates | 02:44:43 08 Oct 2019 | 02:51:56 08 Oct 2019 | 7 minutes 13 seconds |
| Resolving best read alignments | 02:51:56 08 Oct 2019 | 02:56:29 08 Oct 2019 | 4 minutes 33 seconds |
| Creating BAM files | 02:56:29 08 Oct 2019 | 03:00:38 08 Oct 2019 | 4 minutes 9 seconds |
| Tabulating error counts | 03:00:38 08 Oct 2019 | 03:02:42 08 Oct 2019 | 2 minutes 4 seconds |
| Re-calibrating base error rates | 03:02:42 08 Oct 2019 | 03:02:44 08 Oct 2019 | 2 seconds |
| Examining read alignment evidence | 03:02:44 08 Oct 2019 | 03:21:08 08 Oct 2019 | 18 minutes 24 seconds |
| Polymorphism statistics | 03:21:08 08 Oct 2019 | 03:21:08 08 Oct 2019 | 0 seconds |
| Output | 03:21:08 08 Oct 2019 | 03:22:33 08 Oct 2019 | 1 minute 25 seconds |
| Total | 1 hour 7 minutes 40 seconds | ||
