Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,779,888 G→A 5.2% A280V (GCA→GTA)  arcD_2 ← Arginine/ornithine antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,779,8880GA 5.2% 1313.2 / 9.9 390A280V (GCA→GTA) arcD_2Arginine/ornithine antiporter
Reads supporting (aligned to +/- strand):  ref base G (167/198);  new base A (23/2);  total (190/200)
Fisher's exact test for biased strand distribution p-value = 3.75e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 400 total aligned reads displayed.

GCAAGCAGTGTCCATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCTAATACAGTTAATAAGAAATAGA  >  USA300TCH1516_ALE/2779798‑2779978
                                                                                          |                                                                                          
gcaagcaGTGTCCATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTg                                                                                            <  1:60919/91‑1 (MQ=255)
 caagcaGTGTCCATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGc                                                                                           >  1:4466533/1‑91 (MQ=255)
  aagcaGTTTACATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCa                                                                                          <  1:4105002/91‑1 (MQ=255)
  aagcaGTGTCCATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCa                                                                                          <  1:2964430/91‑1 (MQ=255)
  aagcaGTGTCCATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCa                                                                                          >  2:4805582/1‑91 (MQ=255)
   agcaGTGTCCATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATCGTTGCaa                                                                                         >  2:5586640/1‑91 (MQ=255)
   agcaGTGTCCATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCaa                                                                                         <  2:572413/91‑1 (MQ=255)
   agcaGTGTCCATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCaa                                                                                         <  2:6122773/91‑1 (MQ=255)
    gcaGTGTCCATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAg                                                                                        <  2:5127137/91‑1 (MQ=255)
     caGTGTCCATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTCCAATAGTTGCAAGc                                                                                       >  2:5352814/1‑91 (MQ=255)
     caGTGTCCATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGc                                                                                       <  1:4789851/91‑1 (MQ=255)
     caGTGTCCATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGc                                                                                       <  1:6661045/91‑1 (MQ=255)
     caGTGTCCATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGc                                                                                       >  1:5426738/1‑91 (MQ=255)
      aGTGTCCATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCa                                                                                      >  1:1635937/1‑91 (MQ=255)
       gtgtCCATCCTAACCATGCACCTAGTACCGAAATAATTAAACCGATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCAc                                                                                     >  1:5463449/1‑91 (MQ=255)
         gtCCATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCt                                                                                   >  1:2294451/1‑91 (MQ=255)
           ccATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCtgt                                                                                 <  2:665717/91‑1 (MQ=255)
           ccATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCtgt                                                                                 >  1:2909165/1‑91 (MQ=255)
             aTCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGc                                                                               <  2:2308876/91‑1 (MQ=255)
             aTCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGc                                                                               >  1:3115276/1‑91 (MQ=255)
             aTCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGc                                                                               <  2:2697049/91‑1 (MQ=255)
              tCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACGACGCCCAATAGTTGCAAGCACCTGTGcc                                                                              >  2:2019484/1‑91 (MQ=255)
              tCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACGCCCAATCATTACAAACACCAGTGcc                                                                              >  2:1954654/1‑91 (MQ=255)
              tCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGcc                                                                              <  1:6610074/91‑1 (MQ=255)
              tCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGcc                                                                              <  1:5766276/91‑1 (MQ=255)
               ccTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACACGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCa                                                                             >  2:3152616/1‑91 (MQ=255)
                  aaCCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATAc                                                                          >  2:1762516/1‑91 (MQ=255)
                   aCCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACt                                                                         >  1:1381036/1‑91 (MQ=255)
                   aCCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACt                                                                         >  2:4736567/1‑91 (MQ=255)
                   aCCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACt                                                                         >  1:4162152/1‑91 (MQ=255)
                    ccATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACtt                                                                        >  1:5595406/1‑91 (MQ=255)
                    ccATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGCAGATCCCCAACCACCTCCAATAGTTGCAAGCACCTGTGCCATACtt                                                                        >  2:6789753/1‑91 (MQ=255)
                     cATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCTCGGACCACGTCCAATCATTACAAGAAACAGTGCCATACTTg                                                                       >  2:3367636/1‑91 (MQ=255)
                      aTGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTgg                                                                      <  2:1658748/91‑1 (MQ=255)
                        gCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCTCGAACGACCCCGAATAATTAAAAGCAACTGTGCCATACTTGGCg                                                                    >  2:641884/1‑91 (MQ=255)
                        gCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCg                                                                    <  1:6559200/91‑1 (MQ=255)
                          aCCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGaa                                                                  >  1:5892381/1‑91 (MQ=255)
                          aCCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATACTTGCAAGCACCTCTGCCATGCTTGGCGaa                                                                  <  1:6413013/91‑1 (MQ=255)
                                 aCCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCCCCAATAATTACAAGCACCAGTGCCATACTTGGCGAATCTAAtt                                                           >  2:3706246/1‑91 (MQ=255)
                                  ccGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTg                                                          >  1:2015364/1‑91 (MQ=255)
                                   cGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGc                                                         >  1:5018578/1‑91 (MQ=255)
                                   cGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGc                                                         >  1:1952525/1‑91 (MQ=255)
                                       ataatTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGaaa                                                     <  1:1235917/91‑1 (MQ=255)
                                       ataatTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGaaa                                                     <  2:2872706/91‑1 (MQ=255)
                                       ataatTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGaaa                                                     <  2:4994948/91‑1 (MQ=255)
                                       ataatTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGaaa                                                     <  1:2157051/91‑1 (MQ=255)
                                       ataatTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGaaa                                                     >  1:3397498/1‑91 (MQ=255)
                                        taatTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAat                                                    >  1:1854928/1‑91 (MQ=255)
                                        taatTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAat                                                    <  1:2807091/91‑1 (MQ=255)
                                          atTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAatat                                                  >  2:3015252/1‑91 (MQ=255)
                                           ttAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATg                                                 <  2:6354285/91‑1 (MQ=255)
                                            tAAACCAATATTTACAAGTGTAGATCTCGGTGGTCGCCCTATAATTAAAAAAAACTGTGCCATACTTGGCGAATCTTATTGCGAAATATGa                                                >  2:1601670/1‑91 (MQ=255)
                                            tAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCAAAatcata                                                <  1:4727478/91‑5 (MQ=255)
                                             acaCCAATATTTACAAGTGTAGATCGCCAACCACACACAATCATTACAAGCACCTATGCCATACTTGGCGCATCTAATTGCGAAATATGAt                                               >  2:5852345/3‑91 (MQ=255)
                                             aaaCCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGAt                                               >  1:73770/1‑91 (MQ=255)
                                              aaCCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGAtt                                              >  1:4256762/1‑91 (MQ=255)
                                               aCCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGAttt                                             >  2:5516235/1‑91 (MQ=255)
                                                ccAATATTTACAAGTGTAGATCCCCAACCACCTCCAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGAtttt                                            >  2:1413097/1‑91 (MQ=255)
                                                 cAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTg                                           <  2:2427556/91‑1 (MQ=255)
                                                   atatTTACAAGTGTAGATCCCGAAGCACGCCGAATAATTACAAACAACTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCa                                         >  2:3960474/1‑91 (MQ=255)
                                                   atatTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCa                                         >  1:3950872/1‑91 (MQ=255)
                                                    tatTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCaa                                        <  2:4163158/91‑1 (MQ=255)
                                                       ttACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAAt                                     <  1:1464257/91‑1 (MQ=255)
                                                        tACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATc                                    <  1:7763/91‑1 (MQ=255)
                                                        tACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATc                                    <  1:1771967/91‑1 (MQ=255)
                                                        tACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATc                                    <  1:459837/91‑1 (MQ=255)
                                                         aCAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCa                                   <  2:4666450/91‑1 (MQ=255)
                                                          cAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCAc                                  <  2:1130470/91‑1 (MQ=255)
                                                          cAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCAc                                  >  1:4532467/1‑91 (MQ=255)
                                                           aaGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACg                                 <  1:3015252/91‑1 (MQ=255)
                                                              tgtAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCt                              <  2:2201844/91‑1 (MQ=255)
                                                               gtAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCtt                             <  1:2486434/91‑1 (MQ=255)
                                                                   aTCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGc                         <  1:3675839/91‑1 (MQ=255)
                                                                   aTCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGc                         <  2:716038/91‑1 (MQ=255)
                                                                   aTCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGc                         <  1:5491257/91‑1 (MQ=255)
                                                                   aTCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGc                         <  2:3602224/91‑1 (MQ=255)
                                                                   aTCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGc                         <  1:896429/91‑1 (MQ=255)
                                                                    tCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCt                        >  1:6824570/1‑91 (MQ=255)
                                                                    tCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCt                        <  2:4094335/91‑1 (MQ=255)
                                                                    tCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCt                        <  2:5918879/91‑1 (MQ=255)
                                                                    tCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCt                        <  2:348140/91‑1 (MQ=255)
                                                                    tCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCt                        <  1:5142822/91‑1 (MQ=255)
                                                                      cccAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCTaa                      <  2:1198757/91‑1 (MQ=255)
                                                                         aaCCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCTAATAc                   <  2:527058/91‑1 (MQ=255)
                                                                           ccaccTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCTAATACAg                 >  1:533558/1‑91 (MQ=255)
                                                                           ccaccTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCTAATACAg                 <  1:272629/91‑1 (MQ=255)
                                                                               cTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCTAATACAGTtaa             <  1:1565281/91‑1 (MQ=255)
                                                                               cTACAATAGTCGCAAGCACCTGTGCCATACTTGACGAATCTAATTGCGAAAAATGATATTGCAAAATCACGCCTTGAGCTAATACAGTtaa             <  2:5554904/91‑1 (MQ=255)
                                                                                tACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCTAATACAGTtaat            <  1:2095783/91‑1 (MQ=255)
                                                                                tACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCTAATACAGTtaat            >  2:4272705/1‑91 (MQ=255)
                                                                                 aCAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCTAATACAGTtaata           >  1:3334632/1‑91 (MQ=255)
                                                                                 aCAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCTAATACAGTtaata           >  1:6315242/1‑91 (MQ=255)
                                                                                      aGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCTAATACAGTTAATAAGaaa      >  2:884477/1‑91 (MQ=255)
                                                                                      aGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCTAATACAGTTAATAAGaaa      >  1:3819048/1‑91 (MQ=255)
                                                                                      aGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGAGTTTGCAAAATCACGCCTTGAGCTAATACAGTTAATAAGaaa      <  2:6467173/91‑1 (MQ=255)
                                                                                       gTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCTAATACAGTTAATAAGAAAt     >  2:1080169/1‑91 (MQ=255)
                                                                                        ttGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCTAATACAGTTAATAAGAAATa    <  2:2593904/91‑1 (MQ=255)
                                                                                        ttGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCTAATACAGTTAATAAGAAATa    >  2:6830255/1‑91 (MQ=255)
                                                                                        ttGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCTAATACAGTTAATAAGAAATa    <  1:1118695/91‑1 (MQ=255)
                                                                                          gCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCTAATACAGTTAATAAGAAATAGa  <  2:4549813/91‑1 (MQ=255)
                                                                                          |                                                                                          
GCAAGCAGTGTCCATCCTAACCATGCACCTAGTACCGAAATAATTAAACCAATATTTACAAGTGTAGATCCCCAACCACCTACAATAGTTGCAAGCACCTGTGCCATACTTGGCGAATCTAATTGCGAAATATGATTTTGCAAAATCACGCCTTGAGCTAATACAGTTAATAAGAAATAGA  >  USA300TCH1516_ALE/2779798‑2779978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: