breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 71,272 G→T 17.8% A232A (GCC→GCA thiB ← thiamine‑, thiamine pyrophosphate‑, thiamine monophosphate‑binding ABC transporter periplasmic protein; ThiBPQ thiamine, thiamine pyrophosphate importer
RA 93,623 C→A 6.2% T17N (ACC→AAC)  murD → UDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase
JC 101,845 Δ3 bp 8.6% coding (54‑56/1152 nt) ftsZ → GTP‑binding tubulin‑like cell division protein
RA 108,141 G→A 12.4% Q65* (CAG→TAG)  yacG ← DNA gyrase inhibitor
RA 123,694 G→T 15.4% G505C (GGT→TGT)  aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 148,663 G→A 9.9% A19V (GCC→GTC)  yadL ← putative fimbrial‑like adhesin protein
RA 157,254 C→A 6.0% intergenic (‑163/+15) dksA ← / ← sfsA transcriptional regulator of rRNA transcription, DnaK suppressor protein/sugar fermentation stimulation protein A
RA 158,095 C→A 8.4% G142C (GGT→TGT)  ligT ← 2'‑5' RNA ligase
RA 170,808 G→A 6.5% R188C (CGC→TGC)  hemL ← glutamate‑1‑semialdehyde aminotransferase (aminomutase)
RA 241,185 G→T 9.7% P124P (CCG→CCT yafJ → putative amidotransfease
RA 263,261 G→C 6.1% G152G (GGC→GGG yafZ ← CP4‑6 prophage; conserved protein
RA 263,270 C→A 5.6% V149V (GTG→GTT yafZ ← CP4‑6 prophage; conserved protein
RA 263,285 G→A 5.2% D144D (GAC→GAT yafZ ← CP4‑6 prophage; conserved protein
RA 274,938 T→A 11.9% Q152L (CAG→CTG)  insB1 ← IS1 transposase B
RA 302,432 C→T 5.5% A33T (GCA→ACA)  ecpD ← polymerized tip adhesin of ECP fibers
RA 320,638 C→T 5.8% T146T (ACG→ACA ykgH ← putative inner membrane protein
RA 325,307 G→T 6.2% R45L (CGC→CTC)  betT → choline transporter of high affinity
RA 335,730 A→G 5.0% intergenic (+276/‑146) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein
RA 339,232 C→T 5.6% A213V (GCG→GTG)  yahK → aldehyde reductase, NADPH‑dependent, Zn‑containing, broad specificity
RA 349,643 G→T 23.8% L409L (CTG→CTT prpE → propionate‑‑CoA ligase
RA 370,501 G→A 8.2% intergenic (+164/‑414) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,524 T→G 9.3% intergenic (+187/‑391) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,527 C→T 9.1% intergenic (+190/‑388) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 392,189 G→A 6.2% G32D (GGT→GAT)  sbmA → microcin B17 transporter
RA 425,406 C→T 11.1% A149V (GCG→GTG)  secF → SecYEG protein translocase auxillary subunit
RA 459,946 C→T 8.9% A30V (GCC→GTC)  fadM → long‑chain acyl‑CoA thioesterase III
RA 469,361 Δ1 bp 7.8% coding (940/1287 nt) amtB → ammonium transporter
RA 485,431 C→A 7.1% intergenic (+77/+135) mscK → / ← ybaM mechanosensitive channel protein, intermediate conductance, K+ regulated/DUF2496 family protein
RA 515,928 G→A 5.7% W19* (TGG→TGA ybbP → putative ABC transporter permease
RA 546,431 G→T 7.4% V179V (GTG→GTT ybcF → putative carbonate kinase
RA 552,654 C→T 5.5% A182T (GCA→ACA)  folD ← bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase/ 5,10‑methylene‑tetrahydrofolate cyclohydrolase
RA 574,569 G→A 9.3% intergenic (‑220/+71) borD ← / ← ybcV DLP12 prophage; putative lipoprotein/DLP12 prophage; uncharacterized protein
RA 577,301 T→C 6.8% pseudogene (186/438 nt) tfaD → pseudogene, DLP12 prophage; tail fiber assembly protein family;Phage or Prophage Related
RA 612,363 G→T 6.1% L917F (TTG→TTT entF → enterobactin synthase multienzyme complex component, ATP‑dependent
RA 645,627 G→A 6.5% T107I (ACC→ATC)  citE ← citrate lyase, citryl‑ACP lyase (beta) subunit
RA 697,304 C→T 7.8% E302K (GAG→AAG)  nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
RA 707,683 C→A 8.7% P192P (CCG→CCT ybfF ← acyl‑CoA esterase
RA 726,319 G→C 5.4% A427A (GCG→GCC rhsC → Rhs family putative polymorphic toxin
RA 727,369 A→G 5.4% A777A (GCA→GCG rhsC → Rhs family putative polymorphic toxin
RA 727,375 A→G 6.4% G779G (GGA→GGG rhsC → Rhs family putative polymorphic toxin
RA 728,212 G→A 9.8% R1058R (AGG→AGA rhsC → Rhs family putative polymorphic toxin
RA 730,479 C→G 5.6% pseudogene (891/1521 nt) rhsO → pseudogene, Rhs family protein
RA 732,499 T→C 6.3% pseudogene (219/1137 nt) ybfL → pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
RA 749,689 G→A 5.2% T79I (ACC→ATC)  gltA ← citrate synthase
RA 776,600:1 +T 14.0% intergenic (+1/‑3) valZ → / → lysY tRNA‑Val/tRNA‑Lys
RA 797,038 G→T 6.9% R275L (CGC→CTC)  ybhH → putative PrpF family isomerase
RA 808,322 G→A 6.3% P199P (CCG→CCA bioD → dethiobiotin synthetase
RA 830,550 Δ1 bp 10.5% coding (2025/2151 nt) dinG → ATP‑dependent DNA helicase
RA 859,249:1 +C 8.7% coding (152/663 nt) fsaA → fructose‑6‑phosphate aldolase 1
RA 859,251 G→T 8.6% G52C (GGT→TGT)  fsaA → fructose‑6‑phosphate aldolase 1
RA 866,850 C→T 5.2% P143L (CCG→CTG)  gsiC → glutathione transporter, permease component, ABC superfamily
RA 895,178 C→A 6.5% intergenic (+77/+122) rlmC → / ← artJ 23S rRNA m(5)U747 methyltransferase, SAM‑dependent/arginine binding protein, periplasmic
RA 910,659 G→A 9.2% intergenic (‑346/+149) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 968,114 C→T 6.7% W248* (TGG→TGA ycbC ← DUF218 superfamily protein
RA 1,045,283 T→G 11.0% intergenic (‑83/‑6) gfcA ← / → insA O‑antigen capsule production threonine‑rich inner membrane protein/IS1 repressor TnpA
RA 1,067,977 C→A 11.8% D115Y (GAT→TAT)  rutB ← ureidoacrylate amidohydrolase
RA 1,108,540 G→A 9.6% R741H (CGC→CAC)  opgH → membrane glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist
RA 1,186,948 C→T 9.9% P165P (CCG→CCA purB ← adenylosuccinate lyase
RA 1,218,996 G→A 7.3% intergenic (+91/‑24) ymgJ → / → ycgI uncharacterized protein/pseudogene
RA 1,219,001 T→A 7.4% intergenic (+96/‑19) ymgJ → / → ycgI uncharacterized protein/pseudogene
RA 1,253,355 G→A 6.7% intergenic (‑87/+30) ychF ← / ← pth catalase inhibitor protein; ATPase, K+‑dependent, ribosome‑associated/peptidyl‑tRNA hydrolase
RA 1,278,892 C→A 8.3% I1191I (ATC→ATA narG → nitrate reductase 1, alpha subunit
RA 1,282,322 A→G 5.2% intergenic (+340/+200) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,421 A→G 10.3% intergenic (+439/+101) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,430 C→T 10.4% intergenic (+448/+92) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,439 A→G 11.5% intergenic (+457/+83) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,322,638 C→A 7.4% P244P (CCG→CCT yciK ← putative EmrKY‑TolC system oxoacyl‑(acyl carrier protein) reductase
RA 1,420,599 C→A 7.7% intergenic (+260/‑112) ydaY → / → tmpR pseudogene, Rac prophage;Phage or Prophage Related/Rac prophage; pseudogene, tail protein family;Phage or Prophage Related; putative alpha helix protein
RA 1,422,040 A→G 7.5% pseudogene (944/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,060 G→C 11.4% pseudogene (924/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,252 A→G 8.9% pseudogene (732/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,309 T→C 6.4% pseudogene (675/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,627 A→G 8.4% pseudogene (357/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,461,046 C→T 18.9% pseudogene (1398/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,461,055 A→G 19.9% pseudogene (1407/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,470,198 C→A 22.2% D266E (GAC→GAA ydbD → PF10971 family putative periplasmic methylglyoxal resistance protein
RA 1,508,515 G→A 8.1% W143* (TGG→TAG)  ydcU → putative spermidine/putrescine transporter subunit
RA 1,508,959 T→C 7.3% L291P (CTG→CCG)  ydcU → putative spermidine/putrescine transporter subunit
RA 1,513,814 G→A 7.5% V177V (GTG→GTA curA → curcumin/dihydrocurcumin reductase, NADPH‑dependent
RA 1,528,856 G→A 14.8% A192A (GCG→GCA nhoA → N‑hydroxyarylamine O‑acetyltransferase
RA 1,559,660 T→C 7.4% S111G (AGC→GGC)  dosP ← oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator
RA 1,604,344 A→C 12.5% I198M (ATT→ATG uxaB ← altronate oxidoreductase, NAD‑dependent
RA 1,610,132 G→T 14.3% S38I (AGT→ATT)  yneK → uncharacterized protein
RA 1,623,848 C→T 14.0% Q441Q (CAG→CAA ydfI ← putative NAD‑dependent D‑mannonate oxidoreductase
RA 1,652,580 C→A 9.0% S85R (AGC→AGA ynfE → putative selenate reductase, periplasmic
RA 1,681,729 G→A 18.0% R302R (CGC→CGT fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,681,732 A→G 18.0% I301I (ATT→ATC fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,681,735 C→A 17.9% V300V (GTG→GTT fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,683,082 C→T 10.2% L84L (CTG→TTG)  manA → mannose‑6‑phosphate isomerase
RA 1,700,087:1 +C 5.7% coding (64/582 nt) rsxA → SoxR iron‑sulfur cluster reduction factor component; inner membrane protein of electron transport complex
RA 1,702,079 G→A 8.4% E302K (GAA→AAA)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,767,150 G→T 7.3% E128* (GAA→TAA)  ydiN → MFS transporter superfamily protein
RA 1,775,171 C→A 6.2% P172T (CCG→ACG)  ydiR → putative electron transfer flavoprotein, FAD‑binding subunit
RA 1,864,310 C→A 7.9% P367T (CCT→ACT)  yeaH → UPF0229 family protein
RA 1,900,847 C→T 7.6% A157A (GCG→GCA rlmA ← 23S rRNA m(1)G745 methyltransferase, SAM‑dependent
RA 1,919,186 A→G 5.7% I14T (ATC→ACC)  yobA ← CopC family protein
RA 1,933,273 G→A 9.0% L456L (CTG→CTA pykA → pyruvate kinase II
RA 1,996,942 G→T 6.9% A49E (GCG→GAG)  fliC ← flagellar filament structural protein (flagellin)
RA 2,011,900 G→T 9.5% V12F (GTT→TTT)  fliK → flagellar hook‑length control protein
RA 2,055,734 A→T 8.9% intergenic (‑320/‑7) nac ← / → asnV nitrogen assimilation regulon transcriptional regulator; autorepressor/tRNA‑Asn
RA 2,057,140 G→T 6.3% A270D (GCT→GAT)  cobT ← nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase
RA 2,059,656 T→C 10.3% intergenic (‑21/+130) yeeH ← / ← insH1 pseudogene/IS5 transposase and trans‑activator
RA 2,076,426 G→A 6.1% V64I (GTA→ATA)  sbcB → exodeoxyribonuclease I; exonuclease I
RA 2,139,091 C→T 8.3% T782I (ACA→ATA)  yegE → putative diguanylate cyclase
RA 2,171,909 G→A 11.1% R45R (CGC→CGT fbaB ← fructose‑bisphosphate aldolase class I
RA 2,245,164 A→G 22.2% K282K (AAA→AAG nfo → endonuclease IV with intrinsic 3'‑5' exonuclease activity
RA 2,288,125 C→T 8.0% A87T (GCT→ACT)  ccmF ← heme lyase, CcmF subunit
RA 2,335,147 A→G 6.0% D834D (GAT→GAC yfaL ← adhesin
RA 2,335,151 C→G 6.1% G833A (GGC→GCC)  yfaL ← adhesin
RA 2,369,417 A→G 6.7% W9R (TGG→CGG)  menC ← O‑succinylbenzoyl‑CoA synthase
RA 2,397,115 C→T 11.1% L70L (CTG→CTA nuoC ← NADH:ubiquinone oxidoreductase, fused CD subunit
RA 2,412,229 G→T 6.9% P143T (CCG→ACG)  yfcD ← putative NUDIX hydrolase
RA 2,430,734 G→T 5.8% P199P (CCC→CCA pdxB ← erythronate‑4‑phosphate dehydrogenase
RA 2,487,308 G→A 8.0% L192L (CTG→TTG)  yfdX ← uncharacterized protein
RA 2,537,532 C→A 6.3% G151C (GGC→TGC)  ucpA ← furfural resistance protein, putative short‑chain oxidoreductase
RA 2,549,264 G→T 6.7% L166M (CTG→ATG)  eutL ← putative ethanol utilization carboxysome structural protein
RA 2,552,981 G→A 5.9% W255* (TGG→TGA intZ → CPZ‑55 prophage; putative phage integrase
RA 2,597,713:1 +C 5.3% coding (508/948 nt) hyfC → hydrogenase 4, membrane subunit
RA 2,597,714 A→G 5.3% N170S (AAT→AGT) ‡ hyfC → hydrogenase 4, membrane subunit
RA 2,597,715 T→A 5.1% N170K (AAT→AAA) ‡ hyfC → hydrogenase 4, membrane subunit
RA 2,597,717 C→T 5.7% T171M (ACG→ATG)  hyfC → hydrogenase 4, membrane subunit
RA 2,597,723 C→T 5.7% A173V (GCG→GTG)  hyfC → hydrogenase 4, membrane subunit
RA 2,597,993 C→A 5.8% S263* (TCA→TAA)  hyfC → hydrogenase 4, membrane subunit
RA 2,645,634 G→A 6.4% R5C (CGC→TGC)  yfhM ← bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
RA 2,675,209 G→T 8.2% A299E (GCG→GAG)  yphG ← DUF4380 domain‑containing TPR repeat protein
RA 2,719,426 T→C 100% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,719,536 G→T 25.0% noncoding (12/120 nt) rrfG ← 5S ribosomal RNA of rrnG operon
RA 2,748,318 C→A 8.7% H22N (CAC→AAC)  smpB → trans‑translation protein
RA 2,754,345 G→A 11.6% G147D (GGC→GAC)  yfjJ → CP4‑57 prophage; uncharacterized protein
RA 2,805,396 C→A 15.9% T204N (ACC→AAC)  emrA → multidrug efflux system
RA 2,886,451 C→A 8.4% R146S (CGC→AGC)  ygcN → putative oxidoreductase
RA 2,905,169 C→T 14.8% Q614Q (CAG→CAA relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 2,916,898 G→T 11.1% I82I (ATC→ATA truC ← tRNA(Ile1,Asp) pseudouridine(65) synthase
RA 2,960,603 C→T 7.1% P397P (CCG→CCA ptsP ← fused PTS enzyme: PEP‑protein phosphotransferase (enzyme I)/GAF domain containing protein
RA 2,966,898 C→A 8.9% L108L (CTG→CTT lplT ← lysophospholipid transporter
RA 2,968,283 G→T 11.8% P364Q (CCG→CAG)  aas ← fused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase
RA 3,017,915 G→T 6.7% G69V (GGT→GTT)  xanQ → xanthine permease
RA 3,048,677 C→A 12.5% D43Y (GAC→TAC)  ygfB ← UPF0149 family protein
RA 3,062,410 C→A 12.1% V253L (GTG→TTG)  mscS ← mechanosensitive channel protein, small conductance
RA 3,064,708 G→C 14.0% intergenic (‑105/+110) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,064,718 T→G 25.0% intergenic (‑115/+100) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,103,785 C→A 14.7% noncoding (61/76 nt) pheV → tRNA‑Phe
RA 3,150,324 C→A 9.3% H115N (CAT→AAT)  dkgA → 2,5‑diketo‑D‑gluconate reductase A
RA 3,166,880 C→T 5.1% A626T (GCG→ACG)  parE ← DNA topoisomerase IV, subunit B
RA 3,171,284 C→A 7.7% intergenic (‑15/‑190) nudF ← / → tolC ADP‑ribose pyrophosphatase/transport channel
RA 3,202,082 G→A 5.6% D234N (GAC→AAC)  ttdT → L‑tartrate/succinate antiporte
RA 3,203,023 G→A 10.5% R294C (CGT→TGT)  tsaD ← tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein; glycation binding protein
RA 3,203,041 C→A 5.8% G288C (GGC→TGC)  tsaD ← tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein; glycation binding protein
RA 3,275,868 C→A 7.0% T178T (ACC→ACA agaS → tagatose‑6‑phosphate ketose/aldose isomerase
RA 3,300,764 A→C 5.6% G152G (GGT→GGG) ‡ deaD ← ATP‑dependent RNA helicase
RA 3,300,766 C→T 6.2% G152S (GGT→AGT) ‡ deaD ← ATP‑dependent RNA helicase
RA 3,303,762 C→T 10.2% D256N (GAT→AAT)  pnp ← polynucleotide phosphorylase/polyadenylase
RA 3,416,501 C→T 9.1% A236V (GCG→GTG)  yhdZ → putative amino‑acid transporter subunit
RA 3,416,890 T→G 19.4% noncoding (12/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,416,899 A→G 19.2% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,502,647 G→A 7.3% Q176* (CAG→TAG)  yhfW ← phosphopentomutase‑related metalloenzyme superfamily protein
RA 3,511,756 C→A 11.2% P30P (CCG→CCT aroB ← 3‑dehydroquinate synthase
RA 3,532,457 G→T 6.3% A572S (GCC→TCC)  yhgF → putative transcriptional accessory protein
RA 3,564,775 C→T 5.7% T696T (ACG→ACA glgB ← 1,4‑alpha‑glucan branching enzyme
RA 3,597,253 G→A 7.4% D117D (GAC→GAT ftsY ← Signal Recognition Particle (SRP) receptor
RA 3,618,613 T→A 7.4% A292A (GCT→GCA yhhI → putative transposase
RA 3,690,535 C→A 9.9% P240T (CCG→ACG)  bcsE → cellulose production protein
RA 3,695,065 G→A 5.5% S381S (TCG→TCA yhjV → putative transporter
RA 3,732,233 C→A 6.2% V459V (GTC→GTA malS → alpha‑amylase
RA 3,750,846 G→T 7.8% G114G (GGC→GGA yiaY ← putative Fe‑containing alcohol dehydrogenase, Pfam00465 family
RA 3,757,525 T→C 13.2% L661L (CTT→CTC rhsA → Rhs family protein, putative polymorphic toxin; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
RA 3,764,145 C→A 9.6% E199* (GAG→TAG)  yibH ← putative membrane fusion protein (MFP) component of efflux pump
RA 3,792,209 G→T 22.4% S155* (TCA→TAA)  waaU ← lipopolysaccharide core biosynthesis
RA 3,871,524 G→A 7.5% H652H (CAC→CAT gyrB ← DNA gyrase, subunit B
RA 3,918,194 C→A 10.1% G267V (GGG→GTG)  mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
RA 3,973,351 G→T 9.8% L12L (CTG→CTT wecG → UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase
RA 3,973,504 C→A 6.1% N63K (AAC→AAA wecG → UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase
RA 3,982,420 C→A 6.2% E3* (GAA→TAA)  hemX ← putative uroporphyrinogen III methyltransferase
RA 4,096,921 G→A 7.0% W175* (TGG→TGA fieF → ferrous iron and zinc transporter
RA 4,097,962 C→A 6.0% H161Q (CAC→CAA pfkA → 6‑phosphofructokinase I
RA 4,143,586 C→T 10.6% intergenic (‑560/+38) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase
RA 4,154,053 G→T 5.8% G163W (GGG→TGG)  btuB → vitamin B12/cobalamin outer membrane transporter
RA 4,165,450 A→C 13.4% noncoding (51/85 nt) tyrU → tRNA‑Tyr
RA 4,167,038 A→T 7.7% V389V (GTA→GTT tufB → translation elongation factor EF‑Tu 2
RA 4,167,041 A→T 7.6% A390A (GCA→GCT tufB → translation elongation factor EF‑Tu 2
RA 4,178,500 C→T 62.5% R1075C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,208,305 C→A 9.6% T423T (ACC→ACA aceA → isocitrate lyase
RA 4,252,148 C→A 8.9% I184I (ATC→ATA dusA → tRNA‑dihydrouridine synthase A
RA 4,274,144 G→T 14.0% H229Q (CAC→CAA actP ← acetate transporter
RA 4,285,970 C→T 10.2% intergenic (+248/+283) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,286,196 C→T 9.3% intergenic (+474/+57) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,315,681 T→C 5.4% intergenic (‑123/+535) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,315,682 A→G 5.5% intergenic (‑124/+534) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,315,782 A→G 10.0% intergenic (‑224/+434) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,351,888 C→A 6.6% intergenic (‑137/+120) cadC ← / ← yjdQ cadBA operon transcriptional activator/pseudogene, P4‑like integrase remnant
RA 4,362,598 G→T 10.6% intergenic (+110/‑28) groL → / → yjeI Cpn60 chaperonin GroEL, large subunit of GroESL/DUF4156 family lipoprotein
RA 4,367,328 C→A 19.7% G71V (GGC→GTC)  blc ← outer membrane lipoprotein (lipocalin), cell division and growth function
RA 4,408,853 G→A 7.0% A197V (GCG→GTG)  ulaG ← L‑ascorbate 6‑phosphate lactonase
RA 4,498,906 T→C 5.7% pseudogene (81/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,498,909 G→T 5.7% pseudogene (84/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,581 G→A 5.7% V80I (GTT→ATT) 
pseudogene (44/504 nt)
insA →
insB1 →
IS1 repressor TnpA
IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,660 T→G 7.5% pseudogene (123/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,684 C→T 5.4% pseudogene (147/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,690 T→G 6.9% pseudogene (153/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,699 A→G 7.3% pseudogene (162/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,702 T→C 7.6% pseudogene (165/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,897 T→A 7.9% pseudogene (360/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,900 C→T 8.1% pseudogene (363/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,903 T→G 7.5% pseudogene (366/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,511,362 C→A 7.2% G159V (GGC→GTC)  yjhF ← putative transporter
RA 4,539,712 C→T 6.0% intergenic (+185/+58) fimH → / ← gntP minor component of type 1 fimbriae/fructuronate transporter
RA 4,570,144 G→T 14.3% A379D (GCC→GAC)  hsdS ← specificity determinant for hsdM and hsdR

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 578865 579149 285 7 [3] [4] 5 [appY] [appY]
* * ÷ CP009273 1635163 1635298 136 5 [3] [3] 5 essQ/cspB Qin prophage; putative S lysis protein/Qin prophage; cold shock protein
* * ÷ CP009273 2462830 2463094 265 5 [4] [4] 6 gtrS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
* * ÷ CP009273 2463560 2463741 182 5 [4] [2] 6 gtrS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
* * ÷ CP009273 2988772 2988948 177 5 [3] [3] 5 ygeN pseudogene
* * ÷ CP009273 3354183 3354337 155 5 [2] [2] 6 gltD/gltF glutamate synthase, 4Fe‑4S protein, small subunit/periplasmic protein
* * ÷ CP009273 3432042 3432495 454 8 [3] [4] 5 zntR zntR
* * ÷ CP009273 3745275 3745382 108 5 [4] [3] 7 [yiaU] [yiaU]
* * ÷ CP009273 3762969 3763224 256 5 [4] [4] 7 [yibV]–yibV [yibV],yibV
* * ÷ CP009273 3797643 3797741 99 8 [2] [4] 5 waaS lipopolysaccharide core biosynthesis protein
* * ÷ CP009273 3798131 3798498 368 5 [4] [3] 5 [waaS] [waaS]
* * ÷ CP009273 4037430 4037548 119 6 [4] [4] 5 dsbA/yihF periplasmic protein disulfide isomerase I/DUF945 family protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =32 (0.730)37 (0.840) 17/248 NT 53.6% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 1203246 =16 (0.360)18 (0.470) 11/216 NT 58.1% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 12 (0.310)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326114 (0.320)26 (0.680) 14/216 NT 68.2% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 12 (0.310)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 1972854 =4 (0.090)37 (0.840) 17/248 NT 90.2% intergenic (‑400/‑380) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 3576788 = NA (NA)intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA
* ? CP009273 = 19728618 (0.180)48 (1.090) 21/248 NT 85.7% intergenic (‑407/‑373) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E