breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 23,923 C→A 9.4% L511L (CTC→CTA ileS → isoleucyl‑tRNA synthetase
RA 154,164:1 +C 17.1% coding (56/480 nt) folK ← 2‑amino‑4‑hydroxy‑6‑hydroxymethyldihyropteridine pyrophosphokinase
RA 203,315 C→T 18.2% A568V (GCG→GTG)  dnaE → DNA polymerase III alpha subunit
RA 235,604 A→T 12.0% pseudogene (12/273 nt) yafF → pseudogene, H repeat‑associated protein
RA 235,610 G→A 11.8% pseudogene (18/273 nt) yafF → pseudogene, H repeat‑associated protein
RA 235,622 A→G 12.0% pseudogene (30/273 nt) yafF → pseudogene, H repeat‑associated protein
RA 236,251 G→A 7.7% Y142Y (TAC→TAT yafV ← putative NAD(P)‑binding C‑N hydrolase family amidase
RA 259,937 T→G 5.9% R8R (AGG→CGG)  ykfH ← uncharacterized protein
RA 266,165 C→A 11.1% pseudogene (177/258 nt) insN → pseudogene, IS911 transposase A;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 267,666 C→T 5.8% pseudogene (192/426 nt) insN → pseudogene, IS911 transposase A;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 286,409 T→A 11.4% Q152L (CAG→CTG)  insB1 ← IS1 transposase B
RA 310,768 C→A 15.1% pseudogene (701/872 nt) eaeH → pseudogene, attaching and effacing protein homology;factor; Not classified
RA 318,632 A→G 9.8% D195G (GAT→GGT)  ykgF → ferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component
RA 419,377 C→A 8.6% V468V (GTC→GTA malZ → maltodextrin glucosidase
RA 421,095 G→T 10.6% G210V (GGC→GTC)  queA → S‑adenosylmethionine:tRNA ribosyltransferase‑isomerase
RA 456,349 C→T 12.9% P669L (CCG→CTG)  lon → DNA‑binding ATP‑dependent protease La
RA 458,905 C→A 9.6% A512D (GCC→GAC)  ppiD → periplasmic folding chaperone, has an inactive PPIase domain
RA 519,282 C→G 5.8% S189C (TCT→TGT)  rhsD → Rhs family putative polymorphic toxin
RA 638,794 G→T 12.5% intergenic (+12/+219) ybdR → / ← rnk putative Zn‑dependent NAD(P)‑binding oxidoreductase/regulator of nucleoside diphosphate kinase
RA 681,714 G→T 9.5% P138T (CCG→ACG)  gltJ ← glutamate, aspartate ABC transporter permease subunit
RA 726,100 A→G 12.7% R354R (CGA→CGG rhsC → Rhs family putative polymorphic toxin
RA 726,406 C→A 5.6% I456I (ATC→ATA rhsC → Rhs family putative polymorphic toxin
RA 726,453 G→A 6.2% S472N (AGC→AAC)  rhsC → Rhs family putative polymorphic toxin
RA 726,830 G→A 12.3% A598T (GCC→ACC) ‡ rhsC → Rhs family putative polymorphic toxin
RA 726,831 C→T 9.1% A598V (GCC→GTC) ‡ rhsC → Rhs family putative polymorphic toxin
RA 726,848 A→G 7.4% T604A (ACC→GCC)  rhsC → Rhs family putative polymorphic toxin
RA 727,207 C→T 9.0% R723R (CGC→CGT rhsC → Rhs family putative polymorphic toxin
RA 730,555 A→C 7.8% pseudogene (967/1521 nt) rhsO → pseudogene, Rhs family protein
RA 732,071 T→C 12.1% pseudogene (171/240 nt) ybfQ → pseudogene, H repeat‑associated protein
RA 733,602 G→A 9.0% A19T (GCC→ACC)  ybfD → H repeat‑associated putative transposase
RA 752,090 C→A 6.2% P243Q (CCG→CAG)  sdhA → succinate dehydrogenase, flavoprotein subunit
RA 791,151 G→T 15.4% G203W (GGG→TGG)  modA → molybdate transporter subunit
RA 793,168 C→A 12.0% M240I (ATG→ATT ybhA ← pyridoxal phosphate (PLP) phosphatase
RA 845,563 C→A 14.8% intergenic (‑10/‑343) rhtA ← / → ompX threonine and homoserine efflux system/outer membrane protein X
RA 939,824 G→T 11.1% P112P (CCG→CCT dmsC → dimethyl sulfoxide reductase, anaerobic, subunit C
RA 948,489 G→A 9.8% G174G (GGC→GGT pflB ← formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
RA 1,044,051 C→A 9.8% E33* (GAA→TAA)  gfcC ← putative O‑antigen capsule production periplasmic protein
RA 1,045,283 T→G 15.9% intergenic (‑83/‑6) gfcA ← / → insA O‑antigen capsule production threonine‑rich inner membrane protein/IS1 repressor TnpA
RA 1,099,592 G→T 8.2% Q62H (CAG→CAT csgB → curlin nucleator protein, minor subunit in curli complex
RA 1,231,137 C→A 20.0% intergenic (+24/+28) fadR → / ← ycgB fatty acid metabolism regulon transcriptional regulator/SpoVR family stationary phase protein
RA 1,265,353 T→G 6.0% noncoding (40/66 nt) rdlB → sRNA antisense regulator affects LdrB translation; proposed addiction module in LDR‑B repeat, with toxic peptide LdrB
RA 1,282,243 G→A 9.0% intergenic (+261/+279) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,421 A→G 11.3% intergenic (+439/+101) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,430 C→T 12.2% intergenic (+448/+92) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,439 A→G 9.9% intergenic (+457/+83) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,455 Δ1 bp 10.3% intergenic (+473/+67) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,608 T→C 6.9% noncoding (85/171 nt)
T9A (ACT→GCT) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein
RA 1,291,629 C→A 12.3% G650V (GGT→GTT)  adhE ← fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase
RA 1,313,723 G→T 10.8% A108D (GCC→GAC)  trpC ← fused indole‑3‑glycerolphosphate synthetase/N‑(5‑phosphoribosyl)anthranilate isomerase
RA 1,332,470 G→A 12.0% A795T (GCC→ACC)  acnA → aconitate hydratase 1
RA 1,342,483 G→T 11.4% A229A (GCC→GCA rnb ← ribonuclease II
RA 1,351,794 C→A 10.9% M44I (ATG→ATT ymjA ← DUF2543 family protein
RA 1,375,196 G→T 8.5% pseudogene (119/957 nt) ycjV → pseudogene; putative ATP‑binding component of a transport system
RA 1,419,700 A→G 6.5% R23G (AGG→GGG)  ynaK → Rac prophage; conserved protein
RA 1,422,098 G→A 8.7% pseudogene (886/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,150 C→T 5.2% pseudogene (834/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,627 A→G 7.5% pseudogene (357/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,648 G→C 6.1% pseudogene (336/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,491,796 C→A 11.4% T228T (ACC→ACA opgD → osmoregulated periplasmic glucan (OPG) biosynthesis periplasmic protein
RA 1,510,726 G→T 8.7% E298* (GAA→TAA)  patD → gamma‑aminobutyraldehyde dehydrogenase
RA 1,522,374 C→T 6.6% pseudogene (216/2037 nt) rhsE → pseudogene, Rhs family protein
RA 1,523,601 G→A 10.8% pseudogene (1443/2037 nt) rhsE → pseudogene, Rhs family protein
RA 1,523,604 G→A 11.1% pseudogene (1446/2037 nt) rhsE → pseudogene, Rhs family protein
RA 1,527,173 C→T 9.1% Y367Y (TAC→TAT ydcC → H repeat‑associated putative transposase
RA 1,527,189 A→G 15.8% T373A (ACG→GCG)  ydcC → H repeat‑associated putative transposase
RA 1,542,224 A→C 12.8% V189V (GTA→GTC fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
RA 1,543,556 G→A 7.4% L633L (TTG→TTA fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
RA 1,543,952 G→T 10.5% L765L (CTG→CTT fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
RA 1,566,159 G→A 18.2% D48D (GAC→GAT gadB ← glutamate decarboxylase B, PLP‑dependent
RA 1,615,208 C→A 25.0% G63W (GGG→TGG)  eamA ← cysteine and O‑acetyl‑L‑serine efflux system
RA 1,616,734 A→G 11.9% K382K (AAA→AAG ydeE → putative transporter
RA 1,741,170 C→A 20.1% R72R (CGA→AGA)  ydhR → putative monooxygenase
RA 1,763,732 G→A 7.4% G134G (GGG→GGA ydiK → UPF0118 family inner membrane protein
RA 1,776,264 G→T 8.3% E205* (GAG→TAG)  ydiS → putative oxidoreductase
RA 1,825,672 C→A 10.5% E190* (GAA→TAA)  astC ← succinylornithine transaminase, PLP‑dependent
RA 1,838,490 A→G 12.5% N213N (AAT→AAC ynjI ← inner membrane protein
RA 1,946,243 A→G 6.5% I198V (ATC→GTC)  yecE → UPF0759 family protein
RA 1,966,144 G→A 8.0% R269C (CGC→TGC)  tar ← methyl‑accepting chemotaxis protein II
RA 2,067,642 C→A 8.7% L875M (CTG→ATG)  flu → CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
RA 2,068,063 C→T 5.5% A1015V (GCC→GTC)  flu → CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
RA 2,073,561 C→T 17.1% R171H (CGC→CAC)  yeeA ← putative transporter, FUSC family inner membrane protein
RA 2,093,456 G→T 12.9% R432S (CGT→AGT)  gnd ← 6‑phosphogluconate dehydrogenase, decarboxylating
RA 2,194,165 Δ1 bp 8.9% coding (408/3633 nt) yehI → DUF4132 domain‑containing protein
RA 2,215,467 A→G 10.2% M1T (ATG→ACG) † bglX ← beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,242,953 G→A 16.0% L49L (CTG→TTG)  yeiE ← putative DNA‑binding transcriptional regulator
RA 2,286,143 C→A 10.6% V101F (GTC→TTC)  dsbE ← periplasmic thioredoxin of cytochrome c‑type biogenesis
RA 2,298,277 T→C 8.3% intergenic (+405/+310) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,326,086 C→A 7.2% pseudogene (1796/4605 nt) yfaS ← pseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
RA 2,331,055 G→A 7.5% R615R (CGC→CGT gyrA ← DNA gyrase (type II topoisomerase), subunit A
RA 2,355,130 G→A 32.0% G187G (GGC→GGT rhmR ← putative DNA‑binding transcriptional regulator for the rhm operon
RA 2,367,615 C→A 7.3% G290C (GGT→TGT)  menE ← O‑succinylbenzoate‑CoA ligase
RA 2,372,654 C→T 17.4% A29T (GCC→ACC)  menD ← 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase; SEPHCHC synthase
RA 2,385,510 G→A 7.5% A337A (GCC→GCT nuoM ← NADH:ubiquinone oxidoreductase, membrane subunit M
RA 2,386,739 G→A 12.5% A596V (GCA→GTA)  nuoL ← NADH:ubiquinone oxidoreductase, membrane subunit L
RA 2,389,651 G→T 11.2% R93R (CGC→CGA nuoI ← NADH:ubiquinone oxidoreductase, chain I
RA 2,390,812 C→T 8.9% R37H (CGT→CAT)  nuoH ← NADH:ubiquinone oxidoreductase, membrane subunit H
RA 2,410,852 A→G 10.8% E98G (GAA→GGA)  yfcC → putative inner membrane transporter, C4‑dicarboxylate anaerobic carrier family
RA 2,432,260 C→A 7.3% Q278K (CAG→AAG)  flk → putative flagella assembly protein
RA 2,555,743 G→A 6.1% V92I (GTC→ATC)  yffO → CPZ‑55 prophage; uncharacterized protein
RA 2,596,740 C→T 10.2% P522S (CCC→TCC)  hyfB → hydrogenase 4, membrane subunit
RA 2,606,516 G→T 7.8% D420Y (GAC→TAC)  hyfR → hydrogenase‑4 transcriptional activator
RA 2,654,663 G→T 16.1% T76T (ACC→ACA iscS ← cysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent
RA 2,719,426 T→C 80.7% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,761,782 G→T 6.7% Q238H (CAG→CAT yfjP → CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
RA 2,765,683 A→C 11.3% pseudogene (743/900 nt) yfjV ← pseudogene, CP4‑57 prophage;Phage or Prophage Related; putative pump protein
RA 2,765,687 G→C 12.0% pseudogene (739/900 nt) yfjV ← pseudogene, CP4‑57 prophage;Phage or Prophage Related; putative pump protein
RA 2,765,688 G→T 12.0% pseudogene (738/900 nt) yfjV ← pseudogene, CP4‑57 prophage;Phage or Prophage Related; putative pump protein
RA 2,773,187 G→A 5.8% Q967* (CAG→TAG)  ypjA ← adhesin‑like autotransporter
RA 2,784,120 C→T 7.0% I260I (ATC→ATT lhgO → L‑2‑hydroxyglutarate oxidase
RA 2,821,290 G→A 7.3% E65E (GAG→GAA srlD → sorbitol‑6‑phosphate dehydrogenase
RA 2,823,219 G→A 7.2% E16E (GAG→GAA srlQ → D‑arabinose 5‑phosphate isomerase
RA 2,828,862 C→A 8.7% V641V (GTG→GTT hypF ← carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases
RA 2,838,330 C→T 6.9% A501T (GCG→ACG)  hycE ← hydrogenase 3, large subunit
RA 2,910,464 C→A 22.7% I683I (ATC→ATA barA → hybrid sensory histidine kinase, in two‑component regulatory system with UvrY
RA 2,920,857 G→T 13.8% A397A (GCG→GCT ygdH → UPF0717 family protein
RA 2,940,824 Δ1 bp 13.8% intergenic (+2/‑32) metZ → / → metW tRNA‑Met/tRNA‑Met
RA 2,940,826 A→C 19.1% intergenic (+4/‑30) metZ → / → metW tRNA‑Met/tRNA‑Met
RA 2,957,216 C→A 10.3% L107L (CTG→CTT ppdA ← putative prepilin peptidase‑dependent protein
RA 3,012,612 C→T 8.0% V31V (GTC→GTT ssnA → putative chlorohydrolase/aminohydrolase
RA 3,037,885 C→A 6.5% R289S (CGT→AGT)  bglA → 6‑phospho‑beta‑glucosidase A
RA 3,064,718 T→G 18.1% intergenic (‑115/+100) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,070,336 G→T 10.5% H160Q (CAC→CAA yggP ← putative Zn‑binding dehydrogenase
RA 3,120,743 G→T 10.3% S213* (TCG→TAG)  glcD ← glycolate oxidase subunit, FAD‑linked
RA 3,195,411 G→A 5.9% T54T (ACG→ACA cca → fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase
RA 3,202,613 G→A 5.6% A411T (GCT→ACT)  ttdT → L‑tartrate/succinate antiporte
RA 3,234,184 G→T 5.8% L294L (CTG→CTT sstT → sodium:serine/threonine symporter
RA 3,252,730 G→A 7.8% S93S (AGC→AGT tdcG ← L‑serine dehydratase 3, anaerobic
RA 3,263,502 C→T 14.3% intergenic (+541/+73) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,274,855 G→A 6.4% pseudogene (426/555 nt) agaA → pseudogene, N‑acetylgalactosamine‑6‑phosphate deacetylase fragment; putative N‑acetylgalactosamine‑6‑phosphate deacetylase
RA 3,319,397 G→C 9.6% P300A (CCG→GCG)  ftsH ← protease, ATP‑dependent zinc‑metallo
RA 3,332,937 G→T 6.8% P163Q (CCG→CAG)  mlaF ← ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein
RA 3,365,687 C→A 8.7% G83W (GGG→TGG)  nanT ← sialic acid transporter
RA 3,372,344 G→A 14.3% P338L (CCG→CTG)  yhcM ← putative AFG1‑like family P‑loop ATPase
RA 3,401,370 C→A 6.1% F135L (TTC→TTA panF → pantothenate:sodium symporter
RA 3,416,890 T→G 5.6% noncoding (12/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,443,191 A→G 10.8% W18R (TGG→CGG)  rpsC ← 30S ribosomal subunit protein S3
RA 3,457,678 G→T 7.1% R133L (CGG→CTG)  gspL → general secretory pathway component, cryptic
RA 3,477,313 C→T 7.8% D251D (GAC→GAT yheT → UPF0017 family putative hydrolase
RA 3,519,946 C→A 11.1% A40E (GCG→GAG)  yrfF → inner membrane protein
RA 3,528,389 A→G 8.0% I280T (ATC→ACC)  envZ ← sensory histidine kinase in two‑component regulatory system with OmpR
RA 3,615,780 G→A 8.2% A1077T (GCC→ACC)  rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,688,913 C→A 8.2% pseudogene (433/753 nt) bcsQ ← involved in cellulose production, minD superfamily (pseudogene)
RA 3,705,875 C→A 7.9% V140V (GTG→GTT yhjY ← autotransporter beta‑domain protein
RA 3,782,065 G→T 6.8% G202V (GGT→GTT)  yibQ → putative polysaccharide deacetylase
RA 3,785,553 T→C 7.7% E120G (GAA→GGA)  kbl ← glycine C‑acetyltransferase
RA 3,785,736 G→T 10.3% P59H (CCT→CAT)  kbl ← glycine C‑acetyltransferase
RA 3,824,963 C→A 7.4% Q383K (CAA→AAA)  yicH → putative inner membrane‑anchored periplasmic AsmA family protein
RA 3,900,875 G→A 11.4% R22C (CGC→TGC)  phoU ← negative regulator of PhoR/PhoB two‑component regulator
RA 3,945,046 C→T 5.3% pseudogene (145/663 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
RA 3,983,733 G→T 9.1% R132S (CGC→AGC)  hemC ← hydroxymethylbilane synthase
RA 3,988,643 G→A 10.0% T174T (ACG→ACA dapF → diaminopimelate epimerase
RA 4,023,090 G→T 7.3% T414T (ACC→ACA fadB ← fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase
RA 4,075,650 Δ1 bp 7.0% coding (470/903 nt) fdoH ← formate dehydrogenase‑O, Fe‑S subunit
RA 4,075,651 Δ1 bp 7.0% coding (469/903 nt) fdoH ← formate dehydrogenase‑O, Fe‑S subunit
RA 4,141,782 G→T 11.2% I415I (ATC→ATA ppc ← phosphoenolpyruvate carboxylase
RA 4,165,449 C→T 30.9% noncoding (50/85 nt) tyrU → tRNA‑Tyr
RA 4,165,450 A→C 31.0% noncoding (51/85 nt) tyrU → tRNA‑Tyr
RA 4,178,209 C→T 13.2% R978C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,178,500 C→T 100% R1075C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,196,529 T→C 10.5% D311G (GAC→GGC) ‡ purH ← fused IMP cyclohydrolase/phosphoribosylaminoimidazolecarboxamide formyltransferase
RA 4,196,530 C→G 10.5% D311H (GAC→CAC) ‡ purH ← fused IMP cyclohydrolase/phosphoribosylaminoimidazolecarboxamide formyltransferase
RA 4,196,532 G→C 10.5% T310S (ACC→AGC) ‡ purH ← fused IMP cyclohydrolase/phosphoribosylaminoimidazolecarboxamide formyltransferase
RA 4,196,533 T→G 10.2% T310P (ACC→CCC) ‡ purH ← fused IMP cyclohydrolase/phosphoribosylaminoimidazolecarboxamide formyltransferase
RA 4,196,534 T→A 10.3% K309N (AAA→AAT) ‡ purH ← fused IMP cyclohydrolase/phosphoribosylaminoimidazolecarboxamide formyltransferase
RA 4,196,536 T→G 10.3% K309Q (AAA→CAA) ‡ purH ← fused IMP cyclohydrolase/phosphoribosylaminoimidazolecarboxamide formyltransferase
RA 4,221,518 G→T 10.3% R14R (CGC→CGA yjbD ← DUF3811 family protein
RA 4,294,793 G→A 11.8% S541F (TCC→TTC)  yjcS ← metallo‑beta‑lactamase superfamily protein
RA 4,334,762 C→A 6.4% G205V (GGC→GTC)  yjdF ← DUF2238 family inner membrane protein
RA 4,417,684 G→T 11.8% pseudogene (174/402 nt) ytfA → pseudogene, related to transcriptional regulators
RA 4,432,615 G→T 6.7% Q139H (CAG→CAT tamA → translocation and assembly module for autotransporter export, outer membrane subunit
RA 4,508,484 C→T 5.6% A47A (GCC→GCT insA → IS1 repressor TnpA
RA 4,510,272 C→A 12.6% intergenic (‑131/+216) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
RA 4,544,594:1 +C 6.8% coding (202/774 nt) uxuR → fructuronate‑inducible hexuronate regulon transcriptional repressor; autorepressor
RA 4,544,598:1 +G 7.7% coding (206/774 nt) uxuR → fructuronate‑inducible hexuronate regulon transcriptional repressor; autorepressor
RA 4,548,736 C→T 16.4% G203D (GGC→GAC)  iadA ← isoaspartyl dipeptidase
RA 4,593,789 G→T 7.2% G166C (GGT→TGT)  yjjQ → putative transcriptional regulator
RA 4,614,950 C→A 8.5% A80E (GCA→GAA)  serB → 3‑phosphoserine phosphatase
RA 4,625,525 G→T 15.5% G63V (GGT→GTT)  creA → putative periplasmic protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 80533 80629 97 4 [3] [2] 4 leuL/leuO leu operon leader peptide/global transcription factor
* * ÷ CP009273 578971 579310 340 4 [3] [3] 5 [appY] [appY]
* * ÷ CP009273 2027301 2027459 159 4 [2] [3] 6 rseX/yedS sRNA antisense regulator of ompA and ompC translation, Hfq‑dependent/pseudogene, outer membrane protein homology; putative outer membrane protein
* * ÷ CP009273 2622956 2623037 82 5 [3] [3] 4 yfgG/yfgH uncharacterized protein/outer membrane integrity lipoprotein
* * ÷ CP009273 3262621 3262716 96 4 [3] [3] 5 yhaC pentapetide repeats‑related protein
* * ÷ CP009273 3406946 3406981 36 5 [3] [0] 4 acrS/acrE acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
* * ÷ CP009273 3432081 3432489 409 7 [3] [2] 4 zntR zntA gene transcriptional activator
* * ÷ CP009273 3448777 3448837 61 4 [3] [1] 4 gspA/gspC general secretory pathway component, cryptic/general secretory pathway component, cryptic
* * ÷ CP009273 3798342 3798523 182 4 [1] [3] 4 [waaS]–[waaP] [waaS],[waaP]
* * ÷ CP009273 4037417 4037473 57 4 [2] [3] 5 dsbA/yihF periplasmic protein disulfide isomerase I/DUF945 family protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 287112NA (NA)16 (0.460) 9/248 NT 72.9% noncoding (1/768 nt) IS1 repeat region
?CP009273 1972854 = 6 (0.170)intergenic (‑400/‑380) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
* ? CP009273 = 35319431 (1.170)3 (0.120) 3/186 NT 9.0% intergenic (+29/+308) codA/cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
?CP009273 1546506 = NA (NA)noncoding (1/97 nt) RIP121 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP121 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? CP009273 360104 =51 (1.450)21 (0.600) 12/250 NT 29.2% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 = 37038516 (0.630)11 (0.480) 4/164 NT 43.3% intergenic (+48/‑530) mhpE/mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
?CP009273 = 372977 NA (NA)noncoding (187/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? CP009273 1203246 =8 (0.230)13 (0.430) 9/218 NT 65.1% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 7 (0.230)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 12032616 (0.170)15 (0.490) 9/218 NT 71.0% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 7 (0.230)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 19728618 (0.230)29 (0.830) 20/250 NT 78.4% intergenic (‑407/‑373) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E