breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 180,035 C→T 7.5% T362T (ACC→ACT cdaR → carbohydrate diacid regulon transcriptional regulator; autoregulator
RA 221,373:1 +G 6.0% noncoding (1116/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,380 T→A 5.7% noncoding (1123/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,402 A→C 5.3% noncoding (1145/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,407 A→T 5.2% noncoding (1150/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,412 A→T 5.0% noncoding (1155/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,420 A→G 5.5% noncoding (1163/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,425 T→C 8.3% noncoding (1168/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,430 T→C 8.7% noncoding (1173/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,455 G→A 13.4% noncoding (1198/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,464 G→C 11.6% noncoding (1207/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,471 C→T 13.5% noncoding (1214/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 242,746 C→A 5.0% D82Y (GAT→TAT)  dinJ ← antitoxin of YafQ‑DinJ toxin‑antitoxin system
RA 345,428 T→C 5.8% intergenic (+145/‑295) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 407,994 C→T 5.1% intergenic (‑57/+69) araJ ← / ← sbcC arabinose‑inducible putative transporter, MFS family/exonuclease, dsDNA, ATP‑dependent
RA 418,995 T→G 10.1% L341R (CTG→CGG)  malZ → maltodextrin glucosidase
RA 461,228 A→G 5.4% L514P (CTG→CCG)  ybaE ← putative ABC superfamily transporter periplasmic‑binding protein
RA 462,544 C→T 9.1% S75S (TCG→TCA ybaE ← putative ABC superfamily transporter periplasmic‑binding protein
RA 468,294 G→T 5.4% A81S (GCT→TCT)  glnK → nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB
RA 524,606 A→G 5.2% intergenic (+20/‑350) ybbD → / → ylbI pseudogene/pseudogene, Rhs family protein
RA 596,078 C→T 9.1% R637W (CGG→TGG)  cusA → copper/silver efflux system, membrane component
RA 646,936 C→A 8.5% S126I (AGC→ATC)  citC ← [citrate [pro‑3S]‑lyase] ligase
RA 675,003 G→T 9.8% A14S (GCG→TCG)  ybeU → DUF1266 family protein
RA 710,564 C→T 6.9% I517I (ATC→ATT pgm → phosphoglucomutase
RA 732,499 T→C 7.7% pseudogene (219/1137 nt) ybfL → pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
RA 761,369 C→A 8.1% intergenic (‑38/‑71) mngR ← / → mngA Transcriptional repressor for the mannosyl‑D‑glycerate catabolic operon/fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component
RA 917,900 C→A 5.0% Q49H (CAG→CAT cspD ← inhibitor of DNA replication, cold shock protein homolog
RA 978,578 C→T 6.1% G16G (GGC→GGT ycbK → M15A protease‑related family periplasmic protein
RA 1,019,261 C→A 6.2% G27* (GGA→TGA)  yccS ← putative transporter, FUSC superfamily inner membrane protein, tandem domains
RA 1,092,976 T→C 6.6% intergenic (+691/+45) ycdU → / ← serX putative inner membrane protein/tRNA‑Ser
RA 1,094,045 A→G 6.1% D235G (GAC→GGC)  ghrA → glyoxylate/hydroxypyruvate reductase A
RA 1,098,214 G→T 10.8% R147S (CGT→AGT)  csgD ← csgBAC operon transcriptional regulator
RA 1,174,058 G→T 5.0% G4W (GGG→TGG)  nagK → N‑acetyl‑D‑glucosamine kinase
RA 1,203,935 A→G 5.5% E116E (GAA→GAG tfaP → e14 prophage; uncharacterized protein
RA 1,203,941 T→C 6.4% V118V (GTT→GTC tfaP → e14 prophage; uncharacterized protein
RA 1,203,947 A→T 7.0% T120T (ACA→ACT tfaP → e14 prophage; uncharacterized protein
RA 1,203,977 G→T 8.8% T130T (ACG→ACT
A200E (GCG→GAG) 
tfaP →
tfaE ←
e14 prophage; uncharacterized protein
e14 prophage; putative tail fiber assembly protein
RA 1,263,932 C→A 9.7% P104P (CCC→CCA kdsA → 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase
RA 1,269,437 C→T 8.3% F66F (TTC→TTT ychO → putative invasin
RA 1,274,398 C→A 7.2% A329E (GCG→GAG)  narK → nitrate/nitrite transporter
RA 1,282,249 A→G 15.0% intergenic (+267/+273) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,421 A→G 5.2% intergenic (+439/+101) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,353,970 G→T 7.0% P399Q (CCG→CAG)  puuA ← glutamate‑‑putrescine ligase
RA 1,422,040 A→G 11.9% pseudogene (944/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,060 G→C 16.5% pseudogene (924/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,627 A→G 6.3% pseudogene (357/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,447,260 T→C 10.5% S214G (AGT→GGT)  paaZ ← fused oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
RA 1,454,392 C→A 6.2% P361Q (CCG→CAG)  paaH → 3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent
RA 1,460,567 A→G 8.5% pseudogene (919/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,593 C→G 14.8% pseudogene (945/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,602 T→C 18.8% pseudogene (954/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,695 T→G 8.1% pseudogene (1047/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,900 G→A 6.5% pseudogene (1252/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,502,548 G→T 9.5% R504L (CGT→CTT)  ydcP → putative peptidase
RA 1,513,722 C→T 6.1% P147S (CCT→TCT)  curA → curcumin/dihydrocurcumin reductase, NADPH‑dependent
RA 1,527,733 C→A 7.8% E126* (GAG→TAG)  yddH ← flavin reductase like‑protein
RA 1,561,169 G→T 10.8% L77I (CTT→ATT)  dosC ← diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator
RA 1,566,159 G→A 8.7% D48D (GAC→GAT gadB ← glutamate decarboxylase B, PLP‑dependent
RA 1,629,649 T→C 6.2% L152L (TTA→TTG stfQ ← Qin prophage; putative side tail fiber assembly protein
RA 1,629,727 A→G 5.6% V126V (GTT→GTC) ‡ stfQ ← Qin prophage; putative side tail fiber assembly protein
RA 1,629,728 A→G 5.8% V126A (GTT→GCT) ‡ stfQ ← Qin prophage; putative side tail fiber assembly protein
RA 1,629,745 T→A 6.4% A120A (GCA→GCT stfQ ← Qin prophage; putative side tail fiber assembly protein
RA 1,629,762 T→C 8.1% T115A (ACT→GCT)  stfQ ← Qin prophage; putative side tail fiber assembly protein
RA 1,638,422 G→T 5.3% S47Y (TCC→TAC)  ydfU ← Qin prophage; uncharacterized protein
RA 1,647,592 C→T 7.7% R194H (CGC→CAC)  rspB ← putative Zn‑dependent NAD(P)‑binding oxidoreductase
RA 1,671,533 C→A 8.9% E210* (GAA→TAA)  pntA ← pyridine nucleotide transhydrogenase, alpha subunit
RA 1,681,696 A→G 6.4% G313G (GGT→GGC fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,919,123 G→A 5.6% P35L (CCT→CTT)  yobA ← CopC family protein
RA 1,984,659 C→T 7.8% G148S (GGT→AGT)  yecA ← UPF0149 family protein
RA 2,003,340 T→C 5.4% D13D (GAT→GAC yedK → DUF159 family protein
RA 2,022,694 G→A 5.7% R52W (CGG→TGG)  yedI ← DUF808 family inner membrane protein
RA 2,090,755 C→T 6.1% intergenic (+49/+47) hisI → / ← wzzB fused phosphoribosyl‑AMP cyclohydrolase/phosphoribosyl‑ATP pyrophosphatase/regulator of length of O‑antigen component of lipopolysaccharide chains
RA 2,109,077 C→A 7.1% G101C (GGC→TGC)  wcaM ← colanic acid biosynthesis protein
RA 2,186,969:1 +G 6.0% coding (679/1110 nt) mrp ← antiporter inner membrane protein
RA 2,204,389 G→A 6.7% pseudogene (1811/2001 nt) yehQ → pseudogene
RA 2,208,347 G→T 7.9% M1M (ATG→ATT) † mlrA → transcriptional activator of csgD and csgBA
RA 2,212,588 G→A 6.1% P125S (CCT→TCT)  osmF ← putative ABC superfamily transporter periplasmic‑binding protein
RA 2,222,669 T→A 13.5% intergenic (+125/+248) yohP → / ← dusC uncharacterized protein/tRNA‑dihydrouridine synthase C
RA 2,282,187 C→A 8.9% pseudogene (207/2525 nt) yejO ← pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
RA 2,298,128 C→T 9.1% intergenic (+256/+459) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,352,939 C→A 5.3% L231L (CTG→CTT rhmT ← putative L‑rhamnonate transporter
RA 2,442,047 G→T 7.3% intergenic (‑128/‑38) prmB ← / → smrB N5‑glutamine methyltransferase/putative DNA endonuclease
RA 2,465,390 T→G 17.0% pseudogene (212/579 nt) yfdL ← pseudogene, CPS‑53 (KpLE1) prophage;Phage or Prophage Related
RA 2,465,398 C→A 17.6% pseudogene (204/579 nt) yfdL ← pseudogene, CPS‑53 (KpLE1) prophage;Phage or Prophage Related
RA 2,465,404 G→A 17.4% pseudogene (198/579 nt) yfdL ← pseudogene, CPS‑53 (KpLE1) prophage;Phage or Prophage Related
RA 2,465,408 A→G 21.2% pseudogene (194/579 nt) yfdL ← pseudogene, CPS‑53 (KpLE1) prophage;Phage or Prophage Related
RA 2,501,962 T→C 6.5% Q315R (CAG→CGG)  glk ← glucokinase
RA 2,520,778 G→T 6.2% G158G (GGG→GGT yfeH → putative inorganic ion transporter
RA 2,537,074 C→T 7.7% intergenic (‑187/+117) cysP ← / ← ucpA thiosulfate‑binding protein/furfural resistance protein, putative short‑chain oxidoreductase
RA 2,537,077 A→C 7.8% intergenic (‑190/+114) cysP ← / ← ucpA thiosulfate‑binding protein/furfural resistance protein, putative short‑chain oxidoreductase
RA 2,537,086 G→A 6.4% intergenic (‑199/+105) cysP ← / ← ucpA thiosulfate‑binding protein/furfural resistance protein, putative short‑chain oxidoreductase
RA 2,537,810 G→A 6.2% A58V (GCG→GTG)  ucpA ← furfural resistance protein, putative short‑chain oxidoreductase
RA 2,570,278 C→A 5.1% G487C (GGC→TGC)  maeB ← malic enzyme: putative oxidoreductase/putative phosphotransacetylase
RA 2,631,157 C→A 10.9% S285S (TCG→TCT bamB ← BamABCDE complex OM biogenesis lipoprotein
RA 2,708,742 C→T 9.1% intergenic (‑63/‑40) yfiE ← / → eamB putative DNA‑binding transcriptional regulator/cysteine and O‑acetylserine exporter
RA 2,719,426 T→C 100% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,771,760 A→G 7.0% R1442R (CGT→CGC ypjA ← adhesin‑like autotransporter
RA 2,823,222 G→A 6.8% L17L (TTG→TTA srlQ → D‑arabinose 5‑phosphate isomerase
RA 2,881,921 C→T 6.2% R513H (CGT→CAT)  cysI ← sulfite reductase, beta subunit, NAD(P)‑binding, heme‑binding
RA 2,891,408:1 +A 5.0% coding (1370/1455 nt) ygcU ← putative FAD‑containing dehydrogenase
RA 2,891,408:2 +G 5.0% coding (1370/1455 nt) ygcU ← putative FAD‑containing dehydrogenase
RA 2,891,408:3 +A 5.0% coding (1370/1455 nt) ygcU ← putative FAD‑containing dehydrogenase
RA 2,891,408:4 +C 5.0% coding (1370/1455 nt) ygcU ← putative FAD‑containing dehydrogenase
RA 2,958,491 C→G 13.5% M8I (ATG→ATC thyA ← thymidylate synthetase
RA 2,975,338 C→A 6.2% M68I (ATG→ATT araE ← arabinose transporter
RA 3,031,784 G→A 5.5% A133V (GCG→GTG)  dsbC ← protein disulfide isomerase II
RA 3,061,173 C→A 5.6% A89A (GCG→GCT yggE ← oxidative stress defense protein
RA 3,064,718 T→G 13.4% intergenic (‑115/+100) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,129,074 C→G 5.6% A219A (GCG→GCC) ‡ pitB ← phosphate transporter
RA 3,129,075 G→A 5.4% A219V (GCG→GTG) ‡ pitB ← phosphate transporter
RA 3,129,076 C→T 5.4% A219T (GCG→ACG) ‡ pitB ← phosphate transporter
RA 3,129,085 C→G 5.6% V216L (GTT→CTT)  pitB ← phosphate transporter
RA 3,129,086 A→G 5.2% I215I (ATT→ATC pitB ← phosphate transporter
RA 3,227,874:1 +G 9.4% intergenic (‑60/+224) higB ← / ← rlmG mRNA interferase toxin of the HigB‑HigA toxin‑antitoxin system/23S rRNA m(2)G1835 methyltransferase, SAM‑dependent
RA 3,311,602 T→C 6.5% noncoding (47/77 nt) metY ← tRNA‑Met
RA 3,416,890 T→G 6.4% noncoding (12/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,416,899 A→G 11.3% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,457,757 T→C 5.2% I159I (ATT→ATC gspL → general secretory pathway component, cryptic
RA 3,464,590 G→A 5.7% T33T (ACC→ACT tufA ← translation elongation factor EF‑Tu 1
RA 3,508,234 G→A 5.7% T62I (ACC→ATC)  rpe ← D‑ribulose‑5‑phosphate 3‑epimerase
RA 3,518,082 C→G 5.0% P618R (CCG→CGG)  mrcA → fused penicillin‑binding protein 1a: murein transglycosylase/murein transpeptidase
RA 3,617,887 T→C 8.1% F50F (TTT→TTC yhhI → putative transposase
RA 3,643,034 C→A 7.2% P247Q (CCG→CAG)  arsB → arsenite/antimonite transporter
RA 3,662,519 G→A 8.4% I10I (ATC→ATT yhjA ← putative cytochrome C peroxidase
RA 3,666,026 C→A 7.5% Q85K (CAG→AAG)  yhjC → LysR family putative transcriptional regulator
RA 3,668,978 C→T 6.3% A278V (GCC→GTC)  yhjE → MFS superfamily sugar transport 1 family protein
RA 3,679,022 C→A 6.1% intergenic (‑44/+38) yhjK ← / ← bcsC cyclic‑di‑GMP phosphodiesterase/cellulose synthase subunit
RA 3,745,637 G→A 8.5% G96S (GGT→AGT)  yiaU → putative DNA‑binding transcriptional regulator
RA 3,758,716 A→G 5.4% R1058R (AGA→AGG rhsA → Rhs family protein, putative polymorphic toxin; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
RA 3,813,378 C→A 5.4% A385S (GCA→TCA)  ligB ← DNA ligase, NAD(+)‑dependent
RA 3,821,284 A→C 5.9% F248V (TTC→GTC)  gltS ← glutamate transporter
RA 3,887,508 G→T 6.5% G90C (GGC→TGC)  yieE → phosphopantetheinyl transferase superfamily protein
RA 3,911,412 C→A 7.1% D72Y (GAC→TAC)  atpG ← F1 sector of membrane‑bound ATP synthase, gamma subunit
RA 3,963,558 G→T 7.5% A22A (GCG→GCT wecB → UDP‑N‑acetyl glucosamine‑2‑epimerase
RA 4,097,676 G→A 7.4% G66D (GGT→GAT)  pfkA → 6‑phosphofructokinase I
RA 4,100,237 C→T 5.9% A127V (GCG→GTG)  cdh → CDP‑diacylglycerol phosphotidylhydrolase
RA 4,102,717 G→T 9.1% intergenic (+34/‑178) yiiR → / → yiiS DUF805 family putative inner membrane protein/UPF0381 family protein
RA 4,143,463 T→C 7.3% intergenic (‑437/+161) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase
RA 4,173,025 A→T 54.0% Q618L (CAG→CTG)  rpoB → RNA polymerase, beta subunit
RA 4,178,500 C→T 22.4% R1075C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,208,093 G→T 6.9% A353S (GCA→TCA)  aceA → isocitrate lyase
RA 4,208,441 T→G 11.0% intergenic (+100/‑83) aceA → / → aceK isocitrate lyase/isocitrate dehydrogenase kinase/phosphatase
RA 4,293,513 G→T 5.5% R138R (CGC→CGA mdtN ← membrane fusion protein of efflux pump
RA 4,352,388 G→T 7.4% noncoding (56/76 nt) pheU ← tRNA‑Phe
RA 4,379,302 C→T 6.8% T292T (ACG→ACA psd ← phosphatidylserine decarboxylase
RA 4,416,123 G→A 5.4% A67T (GCT→ACT)  rplI → 50S ribosomal subunit protein L9
RA 4,525,233 C→T 5.8% pseudogene (414/1029 nt) yjhR → pseudogene, helicase family; putative frameshift suppressor
RA 4,540,087 C→A 5.2% A343S (GCA→TCA)  gntP ← fructuronate transporter
RA 4,558,635 G→C 6.9% intergenic (‑299/‑180) mdtM ← / → yjiP multidrug efflux system protein/pseudogene, transposase_31 family protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 2027277 2027456 180 9 [8] [6] 9 rseX/yedS sRNA antisense regulator of ompA and ompC translation, Hfq‑dependent/pseudogene, outer membrane protein homology; putative outer membrane protein
* * ÷ CP009273 3432085 3432493 409 9 [4] [7] 10 [zntR] [zntR]
* * ÷ CP009273 3448797 3448865 69 10 [8] [2] 10 gspA/gspC general secretory pathway component, cryptic/general secretory pathway component, cryptic
* * ÷ CP009273 3763056 3763264 209 9 [8] [8] 16 [yibV] [yibV]
* * ÷ CP009273 3797644 3798146 503 9 [6] [7] 9 waaS lipopolysaccharide core biosynthesis protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =87 (1.070)100 (1.240) 40/250 NT 53.5% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 372944 =132 (1.630)11 (0.160) 3/212 NT 10.2% noncoding (154/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
?CP009273 4315902 = 81 (1.180)noncoding (284/583 nt) REP325 (repetitive extragenic palindromic) element; contains 12 REP sequences REP325 (repetitive extragenic palindromic) element; contains 12 REP sequences
* ? CP009273 1203246 =39 (0.480)30 (0.430) 19/218 NT 52.2% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 21 (0.300)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326137 (0.460)25 (0.350) 17/218 NT 48.4% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 21 (0.300)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 197277719 (0.230)4 (0.050) 4/250 NT 17.4% intergenic (‑323/‑457) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E
* ? CP009273 1972854 =5 (0.060)36 (0.440) 19/250 NT 87.8% intergenic (‑400/‑380) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 3576788 = NA (NA)intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA
* ? CP009273 = 19728619 (0.110)51 (0.630) 22/250 NT 85.0% intergenic (‑407/‑373) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E