breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 37,053 C→A 6.3% V258L (GTA→TTA)  caiC ← putative crotonobetaine/carnitine‑CoA ligase
RA 208,388 C→A 6.5% H9N (CAT→AAT)  yaeR → putative lyase
RA 221,373:1 +G 7.0% noncoding (1116/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,380 T→A 7.1% noncoding (1123/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,390 Δ1 bp 6.7% noncoding (1133/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,391 G→A 6.7% noncoding (1134/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,393 C→T 7.2% noncoding (1136/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,395 G→A 7.5% noncoding (1138/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,397 C→T 7.1% noncoding (1140/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,398 C→T 7.5% noncoding (1141/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,402 A→C 7.3% noncoding (1145/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,407 A→T 7.8% noncoding (1150/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,412 A→T 7.8% noncoding (1155/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,420 A→G 8.1% noncoding (1163/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,425 T→C 10.5% noncoding (1168/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,430 T→C 10.9% noncoding (1173/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,455 G→A 11.2% noncoding (1198/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,464 G→C 12.9% noncoding (1207/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,471 C→T 12.8% noncoding (1214/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,476 A→T 14.5% noncoding (1219/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 239,307 G→T 5.7% H162N (CAT→AAT)  fadE ← acyl coenzyme A dehydrogenase
RA 249,874 G→C 7.3% intergenic (+226/‑92) yafP → / → ykfJ putative acyl‑CoA transferase/pseudogene
RA 249,908 A→G 9.7% intergenic (+260/‑58) yafP → / → ykfJ putative acyl‑CoA transferase/pseudogene
RA 370,428 T→C 5.8% intergenic (+91/‑487) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,442 T→C 7.5% intergenic (+105/‑473) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 372,808 G→A 5.0% intergenic (+41/+183) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 393,301 C→A 5.2% H403N (CAT→AAT)  sbmA → microcin B17 transporter
RA 405,420 C→A 7.7% R19L (CGT→CTT)  rdgC ← nucleoid‑associated ssDNA and dsDNA binding protein; competitive inhibitor of RecA function
RA 410,344 G→T 5.0% A289E (GCA→GAA)  sbcC ← exonuclease, dsDNA, ATP‑dependent
RA 474,074 C→T 8.3% intergenic (‑1/+163) ylaB ← / ← ylaC putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein
RA 476,665 G→A 9.3% intergenic (‑501/+45) tomB ← / ← acrB Hha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein
RA 641,405 G→T 5.7% T211N (ACC→AAC)  citT ← citrate:succinate antiporter
RA 709,713 G→T 6.9% D234Y (GAT→TAT)  pgm → phosphoglucomutase
RA 727,369 A→G 5.5% A777A (GCA→GCG rhsC → Rhs family putative polymorphic toxin
RA 727,375 A→G 5.3% G779G (GGA→GGG rhsC → Rhs family putative polymorphic toxin
RA 730,138 A→G 8.9% pseudogene (550/1521 nt) rhsO → pseudogene, Rhs family protein
RA 732,050 C→T 9.6% pseudogene (150/240 nt) ybfQ → pseudogene, H repeat‑associated protein
RA 763,964 G→A 6.3% V177V (GTG→GTA mngB → alpha‑mannosidase
RA 776,297:1 +T 7.9% intergenic (+1/‑2) valT → / → lysW tRNA‑Val/tRNA‑Lys
RA 798,866 G→T 5.2% intergenic (+90/‑93) ybhI → / → ybhJ putative transporter/putative hydratase
RA 878,439 C→A 6.9% A136S (GCC→TCC)  ybjG ← undecaprenyl pyrophosphate phosphatase
RA 888,008:1 +T 5.6% coding (586/903 nt) rimK → ribosomal protein S6 modification protein
RA 888,008:2 +C 5.6% coding (586/903 nt) rimK → ribosomal protein S6 modification protein
RA 888,008:3 +A 5.6% coding (586/903 nt) rimK → ribosomal protein S6 modification protein
RA 888,008:4 +G 5.6% coding (586/903 nt) rimK → ribosomal protein S6 modification protein
RA 888,008:5 +T 5.6% coding (586/903 nt) rimK → ribosomal protein S6 modification protein
RA 888,010:1 +C 5.6% coding (588/903 nt) rimK → ribosomal protein S6 modification protein
RA 888,011:1 +C 5.6% coding (589/903 nt) rimK → ribosomal protein S6 modification protein
RA 888,014:1 +C 5.9% coding (592/903 nt) rimK → ribosomal protein S6 modification protein
RA 888,014:2 +C 5.9% coding (592/903 nt) rimK → ribosomal protein S6 modification protein
RA 910,669 A→T 15.2% intergenic (‑356/+139) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 910,682 T→G 9.5% intergenic (‑369/+126) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 979,869 A→T 8.5% intergenic (+116/+106) ycbL → / ← aspC putative metal‑binding enzyme/aspartate aminotransferase, PLP‑dependent
RA 989,498 C→A 5.3% M1M (ATG→ATT) †
*264L (TGA→TTA) 
ssuB ←
ssuC ←
alkanesulfonate transporter subunit
putative alkanesulfonate transporter subunit
RA 1,005,156 C→A 7.0% F619L (TTC→TTA rlmL → fused 23S rRNA m(2)G2445 and m(7)G2069 methyltransferase, SAM‑dependent
RA 1,025,577 C→A 5.3% R20S (CGC→AGC)  yccX → weak acylphosphatase
RA 1,072,494 C→A 5.3% G615G (GGG→GGT putA ← fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
RA 1,074,183 C→A 6.4% E52D (GAG→GAT putA ← fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
RA 1,117,106 G→T 5.4% Q320K (CAA→AAA)  pyrC ← dihydro‑orotase
RA 1,117,873:1 +G 7.9% coding (191/1047 nt) pyrC ← dihydro‑orotase
RA 1,117,873:2 +A 7.9% coding (191/1047 nt) pyrC ← dihydro‑orotase
RA 1,117,873:3 +C 7.9% coding (191/1047 nt) pyrC ← dihydro‑orotase
RA 1,117,873:4 +A 7.9% coding (191/1047 nt) pyrC ← dihydro‑orotase
RA 1,117,873:5 +G 7.9% coding (191/1047 nt) pyrC ← dihydro‑orotase
RA 1,176,208 C→A 6.7% Q172H (CAG→CAT ycfZ ← inner membrane protein
RA 1,191,604 G→A 5.5% A342A (GCG→GCA icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,203,094 A→G 7.8% E46E (GAA→GAG stfP → e14 prophage; uncharacterized protein
RA 1,203,106 G→T 8.4% V50V (GTG→GTT stfP → e14 prophage; uncharacterized protein
RA 1,203,115 A→C 8.1% A53A (GCA→GCC stfP → e14 prophage; uncharacterized protein
RA 1,204,059 C→T 6.4% A173T (GCT→ACT)  tfaE ← e14 prophage; putative tail fiber assembly protein
RA 1,204,068 T→G 6.2% R170R (AGA→CGA)  tfaE ← e14 prophage; putative tail fiber assembly protein
RA 1,204,071 T→A 5.7% T169S (ACC→TCC)  tfaE ← e14 prophage; putative tail fiber assembly protein
RA 1,204,072 T→G 5.7% T168T (ACA→ACC tfaE ← e14 prophage; putative tail fiber assembly protein
RA 1,204,075 T→C 5.7% E167E (GAA→GAG tfaE ← e14 prophage; putative tail fiber assembly protein
RA 1,211,396 C→T 8.3% A51V (GCC→GTC)  ycgZ → RcsB connector protein for regulation of biofilm and acid‑resistance
RA 1,223,578 C→T 5.6% P15L (CCG→CTG)  ycgM → putative isomerase/hydrolase
RA 1,265,505 A→G 6.1% intergenic (+126/+189) rdlB → / ← ldrC sRNA antisense regulator affects LdrB translation; proposed addiction module in LDR‑B repeat, with toxic peptide LdrB/toxic polypeptide, small
RA 1,265,875 T→C 5.6% noncoding (27/68 nt) rdlC → sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC
RA 1,266,001 G→T 8.2% intergenic (+85/+204) rdlC → / ← chaA sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC/calcium/sodium:proton antiporter
RA 1,282,249 A→G 10.3% intergenic (+267/+273) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,497 A→G 5.5% intergenic (+515/+25) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,340,899 G→A 7.4% T34I (ACC→ATC)  gmr ← cyclic‑di‑GMP phosphodiesterase; csgD regulator; modulator of Rnase II stability
RA 1,370,568 G→A 6.6% G93D (GGT→GAT)  ycjR → putative TIM alpha/beta barrel enzyme
RA 1,397,679 C→T 10.8% A382T (GCC→ACC)  abgA ← p‑aminobenzoyl‑glutamate hydrolase, A subunit
RA 1,417,264 G→T 7.9% pseudogene (307/500 nt) ydaW → Rac prophage; pseudogene, DNA‑binding protein family;Phage or Prophage Related
RA 1,422,040 A→G 10.3% pseudogene (944/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,060 G→C 11.7% pseudogene (924/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,798 A→G 5.2% pseudogene (186/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,430,828 C→A 5.0% G108V (GGT→GTT)  ompN ← outer membrane pore protein N, non‑specific
RA 1,460,752 C→T 12.9% pseudogene (1104/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,761 A→G 11.9% pseudogene (1113/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,896 C→T 7.9% pseudogene (1248/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,484,439 C→T 6.3% pseudogene (532/750 nt) gapC ← pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,513,262 G→A 5.7% intergenic (‑159/‑22) mnaT ← / → curA methionine N‑acyltransferase; L‑amino acid N‑acyltransferase/curcumin/dihydrocurcumin reductase, NADPH‑dependent
RA 1,534,215 G→A 6.3% P878L (CCA→CTA)  narZ ← nitrate reductase 2 (NRZ), alpha subunit
RA 1,534,412 C→A 10.1% A812A (GCG→GCT narZ ← nitrate reductase 2 (NRZ), alpha subunit
RA 1,544,807 C→G 5.8% V30V (GTC→GTG fdnH → formate dehydrogenase‑N, Fe‑S (beta) subunit, nitrate‑inducible
RA 1,544,810 A→G 5.8% A31A (GCA→GCG fdnH → formate dehydrogenase‑N, Fe‑S (beta) subunit, nitrate‑inducible
RA 1,544,816 T→C 5.9% L33L (CTT→CTC fdnH → formate dehydrogenase‑N, Fe‑S (beta) subunit, nitrate‑inducible
RA 1,544,826 T→A 7.2% S37T (TCC→ACC)  fdnH → formate dehydrogenase‑N, Fe‑S (beta) subunit, nitrate‑inducible
RA 1,544,867 G→A 8.6% S50S (TCG→TCA fdnH → formate dehydrogenase‑N, Fe‑S (beta) subunit, nitrate‑inducible
RA 1,590,264 C→A 5.8% pseudogene (1981/5323 nt) yneO ← pseudogene, AidA homolog
RA 1,601,074 T→C 6.9% W240R (TGG→CGG)  lsrF → putative autoinducer‑2 (AI‑2) aldolase
RA 1,602,206 T→C 8.0% L202L (TTA→CTA)  tam → trans‑aconitate methyltransferase
RA 1,618,456 C→A 12.3% R100L (CGA→CTA)  ydeI ← hydrogen peroxide resistance OB fold protein; putative periplasmic protein
RA 1,630,430 T→C 7.3% pseudogene (195/528 nt) nohQ ← pseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin
RA 1,681,324 G→T 16.8% G437G (GGC→GGA fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,702,975 G→A 9.8% A600A (GCG→GCA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,703,015 G→C 12.0% E614Q (GAA→CAA)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,733,972 G→T 6.1% R28R (CGC→CGA ydhB ← LysR family putative transcriptional regulator
RA 1,789,902 C→A 6.3% R767L (CGT→CTT)  pheT ← phenylalanine tRNA synthetase, beta subunit
RA 1,834,538 C→A 5.5% T272K (ACG→AAG)  ynjE → molybdopterin synthase sulfurtransferase
RA 1,905,540 G→T 5.0% L336L (CTG→CTT yebQ → putative transporter
RA 1,920,365 C→A 5.2% intergenic (+12/‑12) yobB → / → exoX C‑N hydrolase family protein/exodeoxyribonuclease 10; DNA exonuclease X
RA 1,952,722 C→T 7.0% intergenic (‑333/+55) torY ← / ← cutC TMAO reductase III (TorYZ), cytochrome c‑type subunit/copper homeostasis protein
RA 1,997,356 G→A 6.8% A2T (GCA→ACA)  fliD → flagellar filament capping protein
RA 2,059,656 T→C 5.3% intergenic (‑21/+130) yeeH ← / ← insH1 pseudogene/IS5 transposase and trans‑activator
RA 2,150,043 C→T 7.8% L434F (CTC→TTC)  mdtB → multidrug efflux system, subunit B
RA 2,165,949 G→A 8.7% P136S (CCT→TCT)  gatD ← galactitol‑1‑phosphate dehydrogenase, Zn‑dependent and NAD(P)‑binding
RA 2,169,217 T→C 5.1% N195S (AAT→AGT)  gatZ ← D‑tagatose 1,6‑bisphosphate aldolase 2, subunit
RA 2,170,724 G→T 21.1% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,245,268 C→T 7.1% S30S (AGC→AGT yeiI → putative kinase
RA 2,298,128 C→T 6.4% intergenic (+256/+459) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,327,175 C→A 5.1% pseudogene (707/4605 nt) yfaS ← pseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
RA 2,366,384 G→T 7.3% W5L (TGG→TTG)  arnF → undecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit
RA 2,415,687 G→T 6.2% L167L (CTG→CTT yfcH → putative NAD‑dependent nucleotide‑sugar epimerase
RA 2,454,695 T→C 5.4% intergenic (‑282/‑90) yfcZ ← / → fadL UPF0381 family protein/long‑chain fatty acid outer membrane transporter
RA 2,459,291 C→T 5.3% A171V (GCA→GTA)  yfdC → putative inner membrane protein
RA 2,465,506 G→T 7.5% pseudogene (96/579 nt) yfdL ← pseudogene, CPS‑53 (KpLE1) prophage;Phage or Prophage Related
RA 2,495,072:1 +G 6.7% coding (464/858 nt) ypdC → putative DNA‑binding protein
RA 2,495,072:2 +G 6.7% coding (464/858 nt) ypdC → putative DNA‑binding protein
RA 2,495,072:3 +A 6.7% coding (464/858 nt) ypdC → putative DNA‑binding protein
RA 2,495,072:4 +T 6.7% coding (464/858 nt) ypdC → putative DNA‑binding protein
RA 2,495,072:5 +G 6.7% coding (464/858 nt) ypdC → putative DNA‑binding protein
RA 2,495,072:6 +A 6.7% coding (464/858 nt) ypdC → putative DNA‑binding protein
RA 2,514,609 Δ1 bp 12.5% intergenic (+2/‑3) valY → / → lysV tRNA‑Val/tRNA‑Lys
RA 2,675,504 G→A 5.2% H201Y (CAT→TAT)  yphG ← DUF4380 domain‑containing TPR repeat protein
RA 2,677,519 A→T 9.5% intergenic (+104/+94) yphH → / ← glyA putative DNA‑binding transcriptional regulator/serine hydroxymethyltransferase
RA 2,719,426 T→C 90.1% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,734,100 A→G 5.1% M137T (ATG→ACG)  aroF ← 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible
RA 2,761,364 T→C 6.0% V99A (GTT→GCT)  yfjP → CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
RA 2,761,401 A→G 6.0% Q111Q (CAA→CAG yfjP → CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
RA 2,762,982 C→T 5.3% intergenic (+137/‑80) yfjQ → / → yfjR CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; putative DNA‑binding transcriptional regulator
RA 2,762,984 T→G 8.2% intergenic (+139/‑78) yfjQ → / → yfjR CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; putative DNA‑binding transcriptional regulator
RA 2,776,157 A→G 6.3% intergenic (‑72/+267) ypjA ← / ← pinH adhesin‑like autotransporter/pseudogene, invertase resolvase family
RA 2,846,644 G→T 7.0% G11V (GGC→GTC)  hypE → carbamoyl dehydratase, hydrogenases 1,2,3 maturation protein
RA 2,881,277 T→C 6.4% N132S (AAT→AGT)  cysH ← phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent
RA 2,900,974 G→A 6.7% N109N (AAC→AAT eno ← enolase
RA 2,920,256 C→T 6.2% A197V (GCT→GTT)  ygdH → UPF0717 family protein
RA 3,085,380 T→C 5.5% G48G (GGT→GGC gshB → glutathione synthetase
RA 3,085,382 A→C 5.2% E49A (GAA→GCA)  gshB → glutathione synthetase
RA 3,085,384 G→T 5.1% A50S (GCC→TCC)  gshB → glutathione synthetase
RA 3,188,333 C→T 6.7% intergenic (‑109/+125) sibD ← / ← sibE sRNA antisense regulator of toxic IbsD protein/sRNA antisense regulator of toxic IbsE protein
RA 3,263,315 T→C 8.0% intergenic (+354/+260) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,416,890 T→G 13.4% noncoding (12/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,416,899 A→G 15.1% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,464,590 G→A 6.4% T33T (ACC→ACT tufA ← translation elongation factor EF‑Tu 1
RA 3,490,604 C→A 5.1% T81T (ACC→ACA nirC → nitrite transporter
RA 3,498,226 T→G 13.7% intergenic (+84/+68) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,498,239 A→C 6.6% intergenic (+97/+55) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,498,242 G→C 6.9% intergenic (+100/+52) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,510,110 G→T 7.4% P186T (CCG→ACG)  damX ← cell division protein that binds to the septal ring
RA 3,603,552 G→T 5.9% G80W (GGG→TGG)  dcrB → putative lipoprotein
RA 3,616,121 G→C 5.8% R1190R (CGG→CGC rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,716,387 C→A 5.7% K457N (AAG→AAT glyS ← glycine tRNA synthetase, beta subunit
RA 3,760,577 C→T 6.0% intergenic (+38/‑4) yibA → / → rhsJ HEAT‑domain lethality reduction protein; putative immunity protein for putative polymorphic toxin RhsA/Rhs‑family protein
RA 3,760,578 A→G 6.1% intergenic (+39/‑3) yibA → / → rhsJ HEAT‑domain lethality reduction protein; putative immunity protein for putative polymorphic toxin RhsA/Rhs‑family protein
RA 3,760,579:1 +T 6.1% intergenic (+40/‑2) yibA → / → rhsJ HEAT‑domain lethality reduction protein; putative immunity protein for putative polymorphic toxin RhsA/Rhs‑family protein
RA 3,951,990 G→T 5.7% E221* (GAG→TAG)  ilvC → ketol‑acid reductoisomerase, NAD(P)‑binding
RA 3,979,173 C→T 7.9% P65P (CCG→CCA aslA ← putative Ser‑type periplasmic non‑aryl sulfatase
RA 4,034,209 G→T 5.0% intergenic (+213/+57) rrfA → / ← mobB 5S ribosomal RNA of rrnA operon/molybdopterin‑guanine dinucleotide biosynthesis protein B
RA 4,098,583 G→A 9.6% intergenic (+141/‑179) pfkA → / → sbp 6‑phosphofructokinase I/sulfate transporter subunit
RA 4,165,449 C→T 24.6% noncoding (50/85 nt) tyrU → tRNA‑Tyr
RA 4,165,450 A→C 24.6% noncoding (51/85 nt) tyrU → tRNA‑Tyr
RA 4,166,006 C→T 5.0% R45R (CGC→CGT tufB → translation elongation factor EF‑Tu 2
RA 4,173,132 G→T 6.3% D654Y (GAC→TAC)  rpoB → RNA polymerase, beta subunit
RA 4,178,500 C→T 60.4% R1075C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,228,437 G→A 5.1% P292P (CCG→CCA yjbH → DUF940 family extracellular polysaccharide protein
RA 4,291,026:1 +T 6.6% coding (1870/2052 nt) mdtO ← membrane translocase (MDR) of MdtNOP efflux pump, PET family
RA 4,291,026:2 +A 6.6% coding (1870/2052 nt) mdtO ← membrane translocase (MDR) of MdtNOP efflux pump, PET family
RA 4,291,028:1 +G 6.6% coding (1868/2052 nt) mdtO ← membrane translocase (MDR) of MdtNOP efflux pump, PET family
RA 4,291,030 G→C 6.9% A622A (GCC→GCG mdtO ← membrane translocase (MDR) of MdtNOP efflux pump, PET family
RA 4,292,471 A→G 5.3% F142S (TTC→TCC)  mdtO ← membrane translocase (MDR) of MdtNOP efflux pump, PET family
RA 4,401,174 G→A 5.7% R19H (CGT→CAT)  yjfK → DUF2491 family protein
RA 4,441,597 G→T 5.6% M241I (ATG→ATT ytfR → putative ABC superfamily sugar transporter ATP‑binding subunit
RA 4,441,641 G→T 7.6% R256L (CGA→CTA)  ytfR → putative ABC superfamily sugar transporter ATP‑binding subunit
RA 4,446,477 C→A 5.2% R292R (CGC→CGA mpl → UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
RA 4,468,590 G→A 6.2% A101T (GCA→ACA)  rraB → protein inhibitor of RNase E
RA 4,508,819 G→C 8.7% pseudogene (282/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,826 G→A 5.8% pseudogene (289/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,830 A→G 6.1% pseudogene (293/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,554,869 C→A 11.8% V267F (GTT→TTT)  yjiM ← putative 2‑hydroxyglutaryl‑CoA dehydratase
RA 4,571,437 C→A 5.9% D477Y (GAC→TAC)  hsdM ← DNA methyltransferase M
RA 4,600,751 C→A 5.6% S507R (AGC→AGA prfC → peptide chain release factor RF‑3

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 3432050 3432502 453 16 [14] [13] 15 zntR–[yhdN] zntR,[yhdN]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 335670 =142 (1.530)7 (0.100)
+28 bp
3/192 NT 7.4% noncoding (203/358 nt) REP23 (repetitive extragenic palindromic) element; contains 8 REP sequences REP23 (repetitive extragenic palindromic) element; contains 8 REP sequences
?CP009273 372889 = 85 (0.920)noncoding (99/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? CP009273 360104 =82 (0.890)92 (0.990) 34/248 NT 52.9% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 370378 =NA (NA)20 (0.230) 11/232 NT NA intergenic (+41/‑537) mhpE/mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
?CP009273 372785 = NA (NA)intergenic (+18/+206) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 = 370385169 (2.520)23 (0.380) 6/162 NT 13.1% intergenic (+48/‑530) mhpE/mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
?CP009273 = 372977 NA (NA)noncoding (187/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? CP009273 370386 =NA (NA)13 (0.200) 8/174 NT 14.2% noncoding (1/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 372878 = 86 (1.210)noncoding (88/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? CP009273 370386 =NA (NA)36 (0.600) 12/162 NT 47.1% noncoding (1/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 372978 = 45 (0.670)intergenic (+211/+13) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 = 370414172 (1.860)6 (0.090) 3/174 NT 5.5% noncoding (29/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 = 372848 85 (1.310)noncoding (58/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? CP009273 370479 =110 (1.190)8 (0.130) 6/164 NT 12.0% noncoding (94/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 372986 = 45 (0.740)intergenic (+219/+5) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 370580 =124 (1.340)8 (0.130) 7/164 NT 11.2% noncoding (195/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 372986 = 45 (0.740)intergenic (+219/+5) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 1203246 =21 (0.230)29 (0.360) 18/216 NT 68.0% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 9 (0.110)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326121 (0.230)31 (0.380) 15/216 NT 69.4% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 9 (0.110)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 1972762 =55 (0.590)3 (0.030) 3/248 NT 5.2% intergenic (‑308/‑472) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 2283592 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? CP009273 1972854 =5 (0.050)49 (0.530) 27/248 NT 90.7% intergenic (‑400/‑380) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 3576788 = NA (NA)intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA
* ? CP009273 = 197286110 (0.110)79 (0.850) 32/248 NT 88.8% intergenic (‑407/‑373) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E