breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,420,5890GA41.9% 100.3 / 212.0 155V303V (GTG→GTAcycAD‑alanine/D‑serine/glycine transporter
*CP0092731,198,4320GT35.2% 111.4 / 133.9 128intergenic (‑43/‑48)cohE/croEe14 prophage; repressor protein phage e14/e14 prophage; putative DNA‑binding transcriptional regulator
*CP0092733,310,5020AC33.1% 221.1 / 139.7 151D128E (GAT→GAGnusAtranscription termination/antitermination L factor
*CP0092732,170,7340GT31.9% 187.6 / 74.2 192intergenic (‑51/+257)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
*CP009273104,0410GT28.2% 168.0 / 36.1 104intergenic (+80/‑151)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
*CP0092732,178,9080TG26.6% 243.0 / 28.3 124intergenic (‑128/+95)thiM/rcnRhydoxyethylthiazole kinase/transcriptional repressor of rcnA
*CP009273838,6520TG24.8% 277.1 / 26.0 149F289V (TTT→GTT) rlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
*CP0092734,402,5980CG24.6% 309.5 / 25.3 125S132C (TCT→TGT) yjfMDUF1190 family protein
*CP009273838,6580GT24.3% 242.9 / 26.7 144A291S (GCC→TCC) rlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
*CP0092732,998,3140CA23.8% 249.9 / 13.2 130V278F (GTT→TTT) ygeVputative sigma‑54‑interacting transcriptional activator
*CP009273104,0420AT22.8% 237.7 / 34.7 103intergenic (+81/‑150)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
*CP0092732,998,3050GT22.3% 275.4 / 20.6 157Q281K (CAA→AAA) ygeVputative sigma‑54‑interacting transcriptional activator
*CP0092733,997,3950GC21.4% 337.2 / 20.9 138F48L (TTC→TTGrarDputative chloramphenical resistance permease
*CP009273703,4710TA21.1% 282.4 / 11.0 110intergenic (+258/‑319)glnS/chiPglutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars
*CP009273763,4120CA21.1% 281.3 / 11.7 133P658Q (CCA→CAA) mngAfused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component
*CP0092732,475,2990CA21.0% 323.1 / 25.8 157M119I (ATG→ATTemrYputative multidrug efflux system
*CP00927368,4860TG20.5% 351.4 / 15.6 161L217V (TTG→GTG) yabIDedA family inner membrane protein
*CP0092734,402,6080GT20.5% 268.6 / 11.2 127L135F (TTG→TTTyjfMDUF1190 family protein
*CP009273838,6720GT20.3% 276.4 / 27.4 149M295I (ATG→ATTrlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 776825 =179 (1.020)37 (0.210)
+G
4/266 10.3 18.9% noncoding (1/76 nt) lysZ tRNA‑Lys
?CP009273 = 2514486 140 (0.800)noncoding (214/1345 nt) IS186 repeat region
* ? CP009273 4385920 =146 (0.830)4 (0.020) 3/248 10.5 2.9% coding (39/1338 nt) amiB N‑acetylmuramoyl‑l‑alanine amidase II
?CP009273 = 4385916 133 (0.820)coding (35/1338 nt) amiB N‑acetylmuramoyl‑l‑alanine amidase II
* ? CP009273 = 4138538224 (1.280)3 (0.020) 3/260 10.8 1.3% coding (1656/1734 nt) eptC LPS heptose I phosphoethanolamine transferase
?CP009273 = 4239463 239 (1.400)intergenic (+224/‑19) lamB/malM maltose outer membrane porin (maltoporin)/maltose regulon periplasmic protein
* ? CP009273 = 3619698193 (1.100)5 (0.030) 3/258 10.8 2.6% coding (466/1125 nt) yhhJ inner membrane putative ABC transporter permease
?CP009273 = 3646525 NA (NA)noncoding (66/1195 nt) IS5 repeat region
* ? CP009273 = 110660259 (1.480)3 (0.020) 3/260 10.8 1.4% coding (730/1044 nt) guaC GMP reductase
?CP009273 3743654 = 157 (0.920)coding (209/696 nt) sgbE L‑ribulose‑5‑phosphate 4‑epimerase
* ? CP009273 977243 =145 (0.830)4 (0.020) 3/266 11.0 2.5% coding (741/1848 nt) ldtD murein L,D‑transpeptidase
?CP009273 = 979160 171 (0.980)coding (55/648 nt) ycbL putative metal‑binding enzyme
* ? CP009273 = 416365193 (1.100)3 (0.020) 3/268 11.0 1.4% coding (1319/1320 nt) brnQ branched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val
?CP009273 = 4487133 234 (1.330)pseudogene (642/1266 nt) intB pseudogene, integrase homology;IS, phage, Tn; Phage‑related functions and prophages; KpLE2 phage‑like element; P4‑like integrase