breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 576 C→A 5.7% A80A (GCC→GCA thrA → Bifunctional aspartokinase/homoserine dehydrogenase 1
RA 7,238 G→A 5.1% A241V (GCT→GTT)  yaaJ ← putative transporter
RA 39,017 G→T 6.1% F33L (TTC→TTA caiB ← crotonobetainyl CoA:carnitine CoA transferase
RA 138,073 G→T 6.3% L52L (CTG→CTT hpt → hypoxanthine phosphoribosyltransferase
RA 148,367 C→A 5.5% G118C (GGT→TGT)  yadL ← putative fimbrial‑like adhesin protein
RA 155,747 C→T 6.1% V285I (GTC→ATC)  gluQ ← glutamyl‑Q tRNA(Asp) synthetase
RA 221,425 T→C 6.8% noncoding (1168/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,430 T→C 7.0% noncoding (1173/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 222,173 T→C 6.1% intergenic (+111/‑73) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA of rrnH operon
RA 262,393 C→T 6.0% pseudogene (93/222 nt) ykfL ← pseudogene, CP4‑6 putative prophage remnant;Phage or Prophage Related
RA 263,324 A→G 6.7% G131G (GGT→GGC yafZ ← CP4‑6 prophage; conserved protein
RA 263,333 G→C 6.7% L128L (CTC→CTG yafZ ← CP4‑6 prophage; conserved protein
RA 263,336 C→A 7.5% G127G (GGG→GGT yafZ ← CP4‑6 prophage; conserved protein
RA 274,938 T→A 11.4% Q152L (CAG→CTG)  insB1 ← IS1 transposase B
RA 290,327 G→T 6.0% H62N (CAC→AAC)  yagM ← CP4‑6 prophage; uncharacterized protein
RA 297,505 T→C 6.1% T32A (ACC→GCC)  paoB ← PaoABC aldehyde oxidoreductase, FAD‑containing subunit
RA 297,542 A→G 6.0% A19A (GCT→GCC paoB ← PaoABC aldehyde oxidoreductase, FAD‑containing subunit
RA 342,623 G→T 6.6% A511E (GCG→GAG)  prpR ← propionate catabolism operon regulatory protein
RA 350,138 C→T 5.9% G574G (GGC→GGT prpE → propionate‑‑CoA ligase
RA 356,856 G→T 5.2% *385L (TGA→TTA)  cynX → putative cyanate transporter
RA 415,386 Δ1 bp 5.6% coding (340/1320 nt) brnQ → branched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val
RA 418,995 T→G 12.7% L341R (CTG→CGG)  malZ → maltodextrin glucosidase
RA 506,620 C→A 5.9% P72P (CCG→CCT copA ← copper transporter
RA 519,269 A→C 5.7% I185L (ATA→CTA) ‡ rhsD → Rhs family putative polymorphic toxin
RA 519,271 A→G 5.7% I185M (ATA→ATG) ‡ rhsD → Rhs family putative polymorphic toxin
RA 519,274 G→C 5.8% L186L (CTG→CTC rhsD → Rhs family putative polymorphic toxin
RA 519,282 C→G 9.0% S189C (TCT→TGT)  rhsD → Rhs family putative polymorphic toxin
RA 526,984 G→T 5.4% H232N (CAT→AAT)  allS ← allD operon transcriptional activator
RA 587,971 C→A 5.9% D222Y (GAT→TAT)  nfrB ← bacteriophage N4 receptor, inner membrane subunit
RA 654,655 C→T 8.0% intergenic (+49/+52) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 682,757 G→A 5.6% A149A (GCC→GCT gltI ← glutamate, aspartate binding protein, periplasmic; part of GltJKLI ABC transporter
RA 726,100 A→G 9.0% R354R (CGA→CGG rhsC → Rhs family putative polymorphic toxin
RA 726,110 T→C 10.1% C358R (TGT→CGT)  rhsC → Rhs family putative polymorphic toxin
RA 727,171 T→G 7.0% N711K (AAT→AAG rhsC → Rhs family putative polymorphic toxin
RA 727,174 C→T 6.7% G712G (GGC→GGT rhsC → Rhs family putative polymorphic toxin
RA 727,207 C→T 6.4% R723R (CGC→CGT rhsC → Rhs family putative polymorphic toxin
RA 727,989 C→A 8.5% A984E (GCA→GAA)  rhsC → Rhs family putative polymorphic toxin
RA 728,212 G→A 7.0% R1058R (AGG→AGA rhsC → Rhs family putative polymorphic toxin
RA 728,560 T→C 5.1% G1174G (GGT→GGC rhsC → Rhs family putative polymorphic toxin
RA 776,297:1 +T 5.0% intergenic (+1/‑2) valT → / → lysW tRNA‑Val/tRNA‑Lys
RA 811,084 A→T 15.8% intergenic (+81/+111) uvrB → / ← ybhK excinulease of nucleotide excision repair, DNA damage recognition component/putative NAD(P)‑binding transferase
RA 811,100 G→T 7.3% intergenic (+97/+95) uvrB → / ← ybhK excinulease of nucleotide excision repair, DNA damage recognition component/putative NAD(P)‑binding transferase
RA 819,131 G→T 6.5% P22H (CCC→CAC)  clsB ← cardiolipin synthase 2
RA 824,808 C→A 6.0% L207F (TTG→TTT ybhG ← putative membrane fusion protein (MFP) component of efflux pump, membrane anchor
RA 839,598 C→A 6.1% G447* (GGA→TGA)  ybiO ← mechanosensitive channel protein, intermediate conductance
RA 861,196 C→T 7.3% V209I (GTA→ATA)  moeA ← molybdopterin molybdenumtransferase; molybdopterin biosynthesis protein
RA 910,669 A→T 28.5% intergenic (‑356/+139) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 926,796 A→G 5.3% V237V (GTT→GTC trxB ← thioredoxin reductase, FAD/NAD(P)‑binding
RA 931,037 G→A 6.9% A786A (GCG→GCA ftsK → DNA translocase at septal ring sorting daughter chromsomes
RA 931,040 A→G 6.8% P787P (CCA→CCG ftsK → DNA translocase at septal ring sorting daughter chromsomes
RA 937,807 G→A 22.2% V465I (GTA→ATA)  dmsA → dimethyl sulfoxide reductase, anaerobic, subunit A
RA 984,786:1 +C 11.1% coding (1027/1203 nt) pncB ← nicotinate phosphoribosyltransferase
RA 984,786:2 +A 11.1% coding (1027/1203 nt) pncB ← nicotinate phosphoribosyltransferase
RA 984,786:3 +G 11.1% coding (1027/1203 nt) pncB ← nicotinate phosphoribosyltransferase
RA 984,786:4 +G 11.1% coding (1027/1203 nt) pncB ← nicotinate phosphoribosyltransferase
RA 991,716 C→G 7.7% V229L (GTA→CTA)  ssuA ← aliphatic sulfonate binding protein, SsuABC ABC transporter
RA 1,045,283 T→G 10.3% intergenic (‑83/‑6) gfcA ← / → insA O‑antigen capsule production threonine‑rich inner membrane protein/IS1 repressor TnpA
RA 1,091,663 C→A 6.3% A122E (GCG→GAG)  ycdU → putative inner membrane protein
RA 1,126,333 C→T 7.5% intergenic (‑14/‑141) flgA ← / → flgB assembly protein for flagellar basal‑body periplasmic P ring/flagellar component of cell‑proximal portion of basal‑body rod
RA 1,145,242 C→A 9.5% A20D (GCT→GAT)  fabD → malonyl‑CoA‑[acyl‑carrier‑protein] transacylase
RA 1,159,361 G→T 6.1% D216Y (GAC→TAC)  thiK → thiamine kinase
RA 1,181,824 G→T 7.3% V175V (GTG→GTT pepT → peptidase T
RA 1,203,115 A→C 5.3% A53A (GCA→GCC stfP → e14 prophage; uncharacterized protein
RA 1,218,978 C→T 6.9% intergenic (+73/‑42) ymgJ → / → ycgI uncharacterized protein/pseudogene
RA 1,223,830 C→A 5.9% A99E (GCG→GAG)  ycgM → putative isomerase/hydrolase
RA 1,259,176 C→T 5.7% L3F (CTT→TTT)  hemA → glutamyl tRNA reductase
RA 1,264,647 T→C 30.0% S29G (AGC→GGC)  ldrA ← toxic polypeptide, small
RA 1,264,818 G→T 9.2% noncoding (40/67 nt) rdlA → sRNA antisense regulator affects LdrA translation; proposed addiction module in LDR‑A repeat, with toxic peptide LdrA
RA 1,265,888 T→G 5.7% noncoding (40/68 nt) rdlC → sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC
RA 1,266,001 G→T 8.2% intergenic (+85/+204) rdlC → / ← chaA sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC/calcium/sodium:proton antiporter
RA 1,279,542 C→A 5.3% F161L (TTC→TTA narH → nitrate reductase 1, beta (Fe‑S) subunit
RA 1,282,249 A→G 8.6% intergenic (+267/+273) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,497 A→G 6.2% intergenic (+515/+25) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,302,861 C→A 5.8% L14L (CTG→CTT clsA ← cardiolipin synthase 1
RA 1,307,809 C→A 5.3% G38W (GGG→TGG)  yciC ← UPF0259 family inner membrane protein
RA 1,314,784:1 +C 7.7% coding (858/1596 nt) trpD ← fused glutamine amidotransferase (component II) of anthranilate synthase/anthranilate phosphoribosyl transferase
RA 1,362,759 C→T 6.7% R142C (CGT→TGT)  pspA → regulatory protein for phage‑shock‑protein operon
RA 1,422,040 A→G 20.3% pseudogene (944/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,060 G→C 21.1% pseudogene (924/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,168 A→C 5.0% pseudogene (816/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,252 A→G 6.0% pseudogene (732/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,627 A→G 5.6% pseudogene (357/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,648 G→C 5.5% pseudogene (336/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,426,026 C→T 6.0% G907G (GGC→GGT stfR → Rac prophage; putative tail fiber protein
RA 1,426,087 G→A 6.7% A928T (GCT→ACT)  stfR → Rac prophage; putative tail fiber protein
RA 1,426,200 G→A 6.5% L965L (TTG→TTA stfR → Rac prophage; putative tail fiber protein
RA 1,449,375 C→A 7.1% T84K (ACG→AAG)  paaB → putative ring 1,2‑phenylacetyl‑CoA epoxidase subunit
RA 1,450,821 A→G 5.2% K45K (AAA→AAG paaE → ring 1,2‑phenylacetyl‑CoA epoxidase, NAD(P)H oxidoreductase component
RA 1,460,602 T→C 5.9% pseudogene (954/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,689 A→G 8.1% pseudogene (1041/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,695 T→G 7.3% pseudogene (1047/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,761 A→G 5.3% pseudogene (1113/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,887 G→C 5.1% pseudogene (1239/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,896 C→T 6.8% pseudogene (1248/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,478,291 C→A 10.1% A325E (GCG→GAG)  hrpA → putative ATP‑dependent helicase
RA 1,495,185 A→G 5.5% Y119C (TAT→TGT)  tehA → potassium‑tellurite ethidium and proflavin transporter
RA 1,522,209 C→T 5.2% pseudogene (51/2037 nt) rhsE → pseudogene, Rhs family protein
RA 1,536,113 A→G 5.9% Y245Y (TAT→TAC narZ ← nitrate reductase 2 (NRZ), alpha subunit
RA 1,583,663 T→C 5.2% pseudogene (674/1227 nt) ydeT ← pseudogene; putative outer membrane protein
RA 1,628,897 G→C 6.2% G82G (GGC→GGG tfaQ ← Qin prophage; putative tail fiber assembly protein
RA 1,628,900 G→T 6.2% S81S (TCC→TCA tfaQ ← Qin prophage; putative tail fiber assembly protein
RA 1,628,903 A→C 6.2% A80A (GCT→GCG tfaQ ← Qin prophage; putative tail fiber assembly protein
RA 1,628,906 C→T 6.4% V79V (GTG→GTA tfaQ ← Qin prophage; putative tail fiber assembly protein
RA 1,629,619 G→A 6.4% H162H (CAC→CAT stfQ ← Qin prophage; putative side tail fiber assembly protein
RA 1,667,021 C→A 7.5% Q269K (CAA→AAA)  ydgD → putative peptidase
RA 1,705,195 C→A 6.6% N37K (AAC→AAA rsxE → SoxR iron‑sulfur cluster reduction factor component; electron transport inner membrane NADH‑quinone reductase
RA 1,808,054 G→T 6.9% intergenic (‑134/‑70) cedA ← / → katE cell division modulator/catalase HPII, heme d‑containing
RA 1,889,552 G→T 6.0% R164L (CGG→CTG)  pabB → aminodeoxychorismate synthase, subunit I
RA 1,890,261 C→T 6.6% C400C (TGC→TGT pabB → aminodeoxychorismate synthase, subunit I
RA 1,900,800 C→A 6.8% W173L (TGG→TTG)  rlmA ← 23S rRNA m(1)G745 methyltransferase, SAM‑dependent
RA 1,910,467 C→A 8.1% intergenic (‑82/‑48) msrC ← / → yebS free methionine‑(R)‑sulfoxide reductase/inner membrane protein
RA 1,922,808:1 +A 12.0% coding (289/2061 nt) ptrB ← protease II
RA 1,934,657 C→A 7.9% L412L (CTG→CTT mepM ← murein DD‑endopeptidase, space‑maker hydrolase, septation protein
RA 1,938,416 T→G 6.2% L249R (CTG→CGG)  znuB → zinc transporter subunit: membrane component of ABC superfamily
RA 1,984,190 G→T 6.5% A343A (GCG→GCT tyrP → tyrosine transporter
RA 1,984,666 C→A 5.6% A145A (GCG→GCT yecA ← UPF0149 family protein
RA 2,023,321 T→C 7.9% V101A (GTA→GCA)  yedA → amino acid exporter for phenylalanine, threonine
RA 2,046,345 C→T 6.4% pseudogene (465/1053 nt) yeeL ← pseudogene, glycosyltransferase homology
RA 2,053,685 C→A 5.7% G237G (GGG→GGT cbl ← ssuEADCB/tauABCD operon transcriptional activator
RA 2,059,656 T→C 6.6% intergenic (‑21/+130) yeeH ← / ← insH1 pseudogene/IS5 transposase and trans‑activator
RA 2,170,085 C→T 8.2% W200* (TGG→TAG)  gatY ← D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit
RA 2,170,724 G→T 21.3% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,170,758 C→A 20.0% intergenic (‑75/+233) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,180,931 G→T 6.4% Y321* (TAC→TAA yehA ← putative fimbrial‑like adhesin protein
RA 2,215,365 T→C 10.3% D35G (GAT→GGT)  bglX ← beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,219,620 G→T 5.1% G114V (GGT→GTT) ‡ yohD → DedA family inner membrane protein
RA 2,219,621 T→C 5.1% G114G (GGT→GGC) ‡ yohD → DedA family inner membrane protein
RA 2,219,622 G→T 5.3% A115S (GCC→TCC)  yohD → DedA family inner membrane protein
RA 2,236,832 G→T 12.5% V100V (GTC→GTA folE ← GTP cyclohydrolase I
RA 2,239,031 G→T 5.8% D406E (GAC→GAA cirA ← colicin IA outer membrane receptor and translocator; ferric iron‑catecholate transporter
RA 2,298,241 T→C 8.3% intergenic (+369/+346) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,492 A→G 6.5% intergenic (+620/+95) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,299,172 G→A 5.1% N354N (AAC→AAT mqo ← malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,309,494 G→T 6.2% R843L (CGT→CTT)  rcsD → phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
RA 2,325,320 G→T 7.9% pseudogene (2562/4605 nt) yfaS ← pseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
RA 2,331,882 C→A 5.6% G340C (GGT→TGT)  gyrA ← DNA gyrase (type II topoisomerase), subunit A
RA 2,408,729 C→T 5.1% T168T (ACC→ACT pta → phosphate acetyltransferase
RA 2,514,486 C→A 7.9% intergenic (+1/‑46) valU → / → valY tRNA‑Val/tRNA‑Val
RA 2,514,609 Δ1 bp 9.3% intergenic (+2/‑3) valY → / → lysV tRNA‑Val/tRNA‑Lys
RA 2,566,600 C→A 6.4% G89C (GGC→TGC)  eutD ← phosphate acetyltransferase
RA 2,591,127 G→T 5.3% intergenic (‑150/+63) purC ← / ← bamC phosphoribosylaminoimidazole‑succinocarboxamide synthetase/BamABCDE complex OM biogenesis lipoprotein
RA 2,610,619 C→A 9.2% G389G (GGC→GGA bepA → periplasmic metalloprotease and chaperone for OM protein maintenance and assembly
RA 2,664,683 G→A 8.1% A34T (GCC→ACC)  hcaB → 2,3‑dihydroxy‑2,3‑dihydrophenylpropionate dehydrogenase
RA 2,682,911 A→T 5.4% intergenic (‑46/+119) yfhG ← / ← glrK putative outer membrane protein modulating the QseEF response/sensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR
RA 2,682,952 T→C 5.5% intergenic (‑87/+78) yfhG ← / ← glrK putative outer membrane protein modulating the QseEF response/sensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR
RA 2,682,954 Δ1 bp 5.6% intergenic (‑89/+76) yfhG ← / ← glrK putative outer membrane protein modulating the QseEF response/sensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR
RA 2,682,955 C→A 5.6% intergenic (‑90/+75) yfhG ← / ← glrK putative outer membrane protein modulating the QseEF response/sensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR
RA 2,682,964 A→G 5.4% intergenic (‑99/+66) yfhG ← / ← glrK putative outer membrane protein modulating the QseEF response/sensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR
RA 2,719,426 T→C 17.9% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,748,000 G→T 5.7% Q42K (CAG→AAG)  ratA ← toxic UPF0083 family protein inhibitor of 70S ribosome formation
RA 2,762,416 C→T 5.1% G131G (GGC→GGT yfjQ → CP4‑57 prophage; uncharacterized protein
RA 2,776,157 A→G 5.7% intergenic (‑72/+267) ypjA ← / ← pinH adhesin‑like autotransporter/pseudogene, invertase resolvase family
RA 2,776,199 A→G 6.4% intergenic (‑114/+225) ypjA ← / ← pinH adhesin‑like autotransporter/pseudogene, invertase resolvase family
RA 2,801,533 A→T 11.5% intergenic (+50/‑142) proX → / → ygaY glycine betaine transporter subunit/pseudogene, major facilitator transporter superfamily;putative transport; Not classified; putative transport protein
RA 2,801,537 T→C 7.0% intergenic (+54/‑138) proX → / → ygaY glycine betaine transporter subunit/pseudogene, major facilitator transporter superfamily;putative transport; Not classified; putative transport protein
RA 2,837,487 G→T 5.5% A28E (GCG→GAG)  hycG ← hydrogenase 3 and formate hydrogenase complex, HycG subunit
RA 2,898,090 G→T 5.4% intergenic (+1357/+16) ygcE → / ← queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
RA 2,925,059 A→G 6.3% K216E (AAA→GAA)  ygdG → Ssb‑binding protein, misidentified as ExoIX
RA 3,003,323 G→A 6.0% A401T (GCA→ACA)  ygeY → putative peptidase
RA 3,055,270 C→A 6.0% T354T (ACC→ACA scpA → methylmalonyl‑CoA mutase
RA 3,064,718 T→G 9.8% intergenic (‑115/+100) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,070,315 C→A 5.0% E167D (GAG→GAT yggP ← putative Zn‑binding dehydrogenase
RA 3,101,778 C→T 5.2% G246D (GGT→GAT)  speC ← ornithine decarboxylase, constitutive
RA 3,107,143 G→T 5.2% S190Y (TCT→TAT)  pppA ← bifunctional prepilin leader peptidase/ methylase
RA 3,187,196:1 +G 8.1% coding (974/1662 nt) yqiK → PHB family membrane protein, function unknown
RA 3,196,223 C→A 5.1% S325Y (TCC→TAC)  cca → fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase
RA 3,197,820 T→C 5.0% K43K (AAA→AAG folB ← bifunctional dihydroneopterin aldolase/dihydroneopterin triphosphate 2'‑epimerase
RA 3,200,275 G→T 5.6% A152S (GCA→TCA)  ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,203,935 T→G 5.2% intergenic (‑33/‑205) tsaD ← / → rpsU tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein; glycation binding protein/30S ribosomal subunit protein S21
RA 3,243,906 G→T 9.1% intergenic (+171/‑15) yqjK → / → yqjF uncharacterized protein/putative quinol oxidase subunit
RA 3,353,190 G→T 5.2% E212* (GAA→TAA)  gltD → glutamate synthase, 4Fe‑4S protein, small subunit
RA 3,379,348 C→A 6.3% L47L (CTG→CTT yhcO ← putative barnase inhibitor
RA 3,392,368 G→T 7.0% Q324K (CAG→AAG)  mreC ← cell wall structural complex MreBCD transmembrane component MreC
RA 3,416,890 T→G 11.0% noncoding (12/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,416,899 A→G 11.1% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,432,727 G→T 5.5% R48S (CGC→AGC)  yhdN ← DUF1992 family protein
RA 3,482,330 C→A 6.5% V404F (GTT→TTT)  argD ← bifunctional acetylornithine aminotransferase/ succinyldiaminopimelate aminotransferase
RA 3,524,016 T→C 5.5% intergenic (+5/+58) hslO → / ← yhgE heat shock protein Hsp33/DUF4153 family putative inner membrane protein
RA 3,549,511 C→A 5.7% G233V (GGT→GTT)  rtcA ← RNA 3'‑terminal phosphate cyclase
RA 3,590,084 C→A 10.4% P279P (CCG→CCT livK ← leucine transporter subunit
RA 3,612,611 T→C 8.6% V20V (GTT→GTC rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,616,121 G→C 7.1% R1190R (CGG→CGC rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,616,199 A→G 5.1% R1216R (CGA→CGG rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,618,160 A→G 9.5% G141G (GGA→GGG yhhI → putative transposase
RA 3,641,448 T→C 6.2% noncoding (26/84 nt) agrA → Inactive antisense sRNA
RA 3,812,401 C→A 5.7% L56L (CTC→CTA yicG → UPF0126 family inner membrane protein
RA 3,826,590 C→T 6.6% M436I (ATG→ATA yicI ← putative alpha‑glucosidase
RA 3,852,250 C→A 6.3% Q409H (CAG→CAT yidK ← putative transporter
RA 3,893,994 Δ1 bp 5.5% coding (1587/1617 nt) bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,893,995 G→T 5.4% A529D (GCT→GAT) ‡ bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,893,996 C→T 5.2% A529T (GCT→ACT) ‡ bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,893,997 A→T 5.0% F528L (TTT→TTA) ‡ bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,893,998 A→T 5.0% F528Y (TTT→TAT) ‡ bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,893,999 A→T 5.0% F528I (TTT→ATT) ‡ bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,894,001 Δ1 bp 5.3% coding (1580/1617 nt) bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,894,002 G→A 5.3% Q527* (CAG→TAG)  bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,894,003 G→C 5.8% D526E (GAC→GAG) ‡ bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,894,004 T→C 5.8% D526G (GAC→GGC) ‡ bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,894,005 C→A 6.0% D526Y (GAC→TAC) ‡ bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,894,006 G→A 5.8% D525D (GAC→GAT) ‡ bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,894,008 C→G 5.8% D525H (GAC→CAC) ‡ bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,894,010 T→A 5.3% K524I (AAA→ATA) ‡ bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,894,011 T→G 5.7% K524Q (AAA→CAA) ‡ bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,894,013 T→G 5.9% N523T (AAT→ACT) ‡ bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,894,014 T→C 5.6% N523D (AAT→GAT) ‡ bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,894,015 G→A 5.7% D522D (GAC→GAT bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,935,371 G→T 6.7% noncoding (204/1542 nt) rrsC → 16S ribosomal RNA of rrnC operon
RA 3,949,200 G→T 5.6% A170A (GCG→GCT ilvA → L‑threonine dehydratase, biosynthetic; also known as threonine deaminase
RA 4,006,613 C→A 13.9% Y67* (TAC→TAA metE → 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase
RA 4,065,263 A→T 9.5% L250Q (CTG→CAG)  yihT ← putative aldolase
RA 4,143,586 C→T 5.7% intergenic (‑560/+38) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase
RA 4,165,449 C→T 20.1% noncoding (50/85 nt) tyrU → tRNA‑Tyr
RA 4,165,450 A→C 20.3% noncoding (51/85 nt) tyrU → tRNA‑Tyr
RA 4,178,500 C→T 89.2% R1075C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,195,812 G→A 7.3% A16A (GCC→GCT purD ← phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase
RA 4,202,979 A→C 22.2% noncoding (12/120 nt) rrfE → 5S ribosomal RNA of rrnE operon
RA 4,257,184 C→T 9.2% A48V (GCC→GTC)  tyrB → tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent
RA 4,260,762 G→A 5.8% L59L (CTG→CTA yjbR → DUF419 family protein
RA 4,336,238 A→G 9.6% R302R (CGT→CGC fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,336,241 G→A 9.5% I301I (ATC→ATT fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,421,749 C→A 6.2% A18S (GCT→TCT)  ytfE ← iron‑sulfur cluster repair protein RIC
RA 4,435,171 C→A 9.9% Q415K (CAG→AAG)  tamB → translocation and assembly module for autotransporter export, inner membrane subunit
RA 4,440,847 G→T 9.3% intergenic (+112/‑28) ytfQ → / → ytfR galactofuranose binding proteint: periplasmic‑binding component of ABC superfamily/putative ABC superfamily sugar transporter ATP‑binding subunit
RA 4,441,770 G→T 12.0% R299L (CGT→CTT)  ytfR → putative ABC superfamily sugar transporter ATP‑binding subunit
RA 4,498,944 G→A 6.0% pseudogene (119/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,498,951 C→A 5.4% pseudogene (126/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,505,305 A→T 5.6% L397Q (CTG→CAG)  fecA ← ferric citrate outer membrane transporter
RA 4,508,826 G→A 9.6% pseudogene (289/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,830 A→G 9.5% pseudogene (293/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,885 T→A 5.2% pseudogene (348/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,519,008 C→G 5.9% R351S (AGG→AGC sgcC ← putative PTS system EIIC permease component
RA 4,530,419 C→T 7.0% intergenic (‑1101/‑355) nanC ← / → fimB N‑acetylnuraminic acid outer membrane channel protein/tyrosine recombinase/inversion of on/off regulator of fimA
RA 4,575,785 A→T 8.8% pseudogene (848/3567 nt) hsdR ← pseudogene, premature stop codon, host restriction; endonuclease R
RA 4,618,118 C→T 5.3% F329F (TTC→TTT nadR → trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 33571743 (0.520)11 (0.170)
+29 bp
4/188 NT 24.9% noncoding (250/358 nt) REP23 (repetitive extragenic palindromic) element; contains 8 REP sequences REP23 (repetitive extragenic palindromic) element; contains 8 REP sequences
?CP009273 = 372985 NA (NA)intergenic (+218/+6) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 360104 =47 (0.570)67 (0.810) 27/248 NT 58.8% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 = 370385108 (1.790)28 (0.520) 9/162 NT 22.4% intergenic (+48/‑530) mhpE/mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
?CP009273 = 372977 NA (NA)noncoding (187/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? CP009273 370386 =NA (NA)8 (0.150) 5/162 NT 9.5% noncoding (1/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 372978 = 85 (1.410)intergenic (+211/+13) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 370704 =79 (0.950)18 (0.260) 7/210 NT 18.9% noncoding (319/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 372963 = 88 (1.250)noncoding (173/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? CP009273 1203246 =16 (0.190)49 (0.680) 27/216 NT 78.4% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 13 (0.180)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326116 (0.190)33 (0.460) 12/216 NT 71.0% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 13 (0.180)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 2399639139 (1.670)11 (0.130) 7/248 NT 7.3% coding (482/939 nt) lrhA transcriptional repressor of flagellar, motility and chemotaxis genes
?CP009273 = 3124699 NA (NA)intergenic (+146/+1) insH1/yghQ IS5 transposase and trans‑activator/putative inner membrane polysaccharide flippase