breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 33,849 C→A 6.2% T1011T (ACC→ACA carB → carbamoyl‑phosphate synthase large subunit
RA 70,310 C→T 5.1% A233A (GCG→GCA thiP ← thiamine, thiamine pyrophosphate ABC transporter permease; ThiBPQ thiamine, thiamine pyrophosphate importer
RA 74,211 C→A 7.5% T35K (ACA→AAA)  setA → broad specificity sugar efflux system
RA 93,850 G→T 5.3% V93F (GTT→TTT)  murD → UDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase
RA 109,449 G→T 5.3% A86A (GCC→GCA coaE ← dephospho‑CoA kinase
RA 110,770 C→T 6.9% G280G (GGC→GGT guaC → GMP reductase
RA 117,670 C→T 5.4% P123P (CCG→CCA aroP ← aromatic amino acid transporter
RA 121,753 C→T 6.6% Y750Y (TAC→TAT aceE → pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding
RA 133,239 G→T 6.1% R56S (CGT→AGT)  yacC ← PulS_OutS family protein
RA 143,179 C→A 7.1% M1M (ATG→ATT) † panD ← aspartate 1‑decarboxylase
RA 150,031 C→A 13.1% S628I (AGC→ATC)  htrE ← putative outer membrane usher protein
RA 154,300 G→T 5.2% L438L (CTC→CTA pcnB ← poly(A) polymerase
RA 207,910 G→T 5.7% R582L (CGC→CTC)  ldcC → lysine decarboxylase 2, constitutive
RA 211,918 G→A 9.1% E55K (GAA→AAA)  nlpE → lipoprotein involved with copper homeostasis and adhesion
RA 221,373:1 +G 6.1% noncoding (1116/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,380 T→A 6.7% noncoding (1123/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,390 Δ1 bp 6.8% noncoding (1133/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,391 G→A 6.8% noncoding (1134/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,393 C→T 7.2% noncoding (1136/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,395 G→A 7.1% noncoding (1138/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,397 C→T 7.1% noncoding (1140/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,398 C→T 7.1% noncoding (1141/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,402 A→C 6.4% noncoding (1145/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,407 A→T 7.4% noncoding (1150/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,412 A→T 7.7% noncoding (1155/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,420 A→G 7.8% noncoding (1163/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,425 T→C 10.6% noncoding (1168/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,430 T→C 10.7% noncoding (1173/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,455 G→A 12.7% noncoding (1198/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,464 G→C 10.2% noncoding (1207/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,471 C→T 11.9% noncoding (1214/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,476 A→T 13.9% noncoding (1219/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 235,607 G→A 9.4% pseudogene (15/273 nt) yafF → pseudogene, H repeat‑associated protein
RA 249,874 G→C 11.9% intergenic (+226/‑92) yafP → / → ykfJ putative acyl‑CoA transferase/pseudogene
RA 249,908 A→G 11.0% intergenic (+260/‑58) yafP → / → ykfJ putative acyl‑CoA transferase/pseudogene
RA 259,937 T→G 5.3% R8R (AGG→CGG)  ykfH ← uncharacterized protein
RA 260,899 G→A 5.1% H7Y (CAC→TAC)  yafX ← CP4‑6 prophage; uncharacterized protein
RA 260,903 C→T 5.4% T5T (ACG→ACA yafX ← CP4‑6 prophage; uncharacterized protein
RA 261,827 C→A 5.1% Q146H (CAG→CAT yafY ← lipoprotein, inner membrane; overproduction stimulates degP expression; CP4‑6 prophage
RA 262,193 T→C 7.1% R24R (AGA→AGG yafY ← lipoprotein, inner membrane; overproduction stimulates degP expression; CP4‑6 prophage
RA 262,205 T→G 7.8% A20A (GCA→GCC yafY ← lipoprotein, inner membrane; overproduction stimulates degP expression; CP4‑6 prophage
RA 262,209 A→C 6.5% I19S (ATC→AGC)  yafY ← lipoprotein, inner membrane; overproduction stimulates degP expression; CP4‑6 prophage
RA 262,211 G→C 7.5% L18L (CTC→CTG yafY ← lipoprotein, inner membrane; overproduction stimulates degP expression; CP4‑6 prophage
RA 300,827 G→T 5.9% V30V (GTC→GTA ecpE ← ECP production pilus chaperone
RA 328,710 G→T 9.3% G210V (GGA→GTA)  yahA → c‑di‑GMP‑specific phosphodiesterase
RA 354,345 G→T 6.6% T19T (ACC→ACA cynR ← transcriptional activator of cyn operon; autorepressor
RA 361,168 G→T 7.1% V192V (GTC→GTA lacI ← DNA‑binding transcriptional repressor
RA 361,219 G→T 8.5% V175V (GTC→GTA lacI ← DNA‑binding transcriptional repressor
RA 370,428 T→C 10.3% intergenic (+91/‑487) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,442 T→C 5.3% intergenic (+105/‑473) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,576 Δ1 bp 6.1% intergenic (+239/‑339) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,577 T→G 6.3% intergenic (+240/‑338) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,703 A→G 7.1% intergenic (+366/‑212) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 397,578 G→A 6.4% A126T (GCA→ACA)  phoA → bacterial alkaline phosphatase
RA 406,624 Δ1 bp 9.7% intergenic (+116/+129) mak → / ← araJ manno(fructo)kinase/arabinose‑inducible putative transporter, MFS family
RA 406,630 Δ1 bp 6.2% intergenic (+122/+123) mak → / ← araJ manno(fructo)kinase/arabinose‑inducible putative transporter, MFS family
RA 408,640 G→T 6.6% A857E (GCA→GAA)  sbcC ← exonuclease, dsDNA, ATP‑dependent
RA 466,823 C→A 6.6% T244T (ACC→ACA mdlB → putative multidrug transporter subunit of ABC superfamily, ATP‑binding component
RA 474,126 A→G 5.5% intergenic (‑53/+111) ylaB ← / ← ylaC putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein
RA 523,625 G→T 5.8% pseudogene (218/708 nt) rhsH → pseudogene, Rhs familly protein
RA 541,156 C→A 6.0% G222W (GGG→TGG)  allD ← ureidoglycolate dehydrogenase
RA 574,088 G→T 7.6% P88T (CCG→ACG)  borD ← DLP12 prophage; putative lipoprotein
RA 634,737 G→A 6.1% E113K (GAA→AAA)  ahpC → alkyl hydroperoxide reductase, C22 subunit
RA 634,739:1 +T 6.5% coding (339/564 nt) ahpC → alkyl hydroperoxide reductase, C22 subunit
RA 634,740 G→A 6.2% D114N (GAT→AAT) ‡ ahpC → alkyl hydroperoxide reductase, C22 subunit
RA 634,742 T→C 6.1% D114D (GAT→GAC) ‡ ahpC → alkyl hydroperoxide reductase, C22 subunit
RA 634,745 A→T 6.3% E115D (GAA→GAT ahpC → alkyl hydroperoxide reductase, C22 subunit
RA 634,746:1 +C 6.2% coding (346/564 nt) ahpC → alkyl hydroperoxide reductase, C22 subunit
RA 634,749:1 +A 6.2% coding (349/564 nt) ahpC → alkyl hydroperoxide reductase, C22 subunit
RA 644,710 C→T 8.3% P106P (CCG→CCA citF ← citrate lyase, citrate‑ACP transferase (alpha) subunit
RA 645,249 T→C 6.9% N233S (AAC→AGC)  citE ← citrate lyase, citryl‑ACP lyase (beta) subunit
RA 654,946 C→A 5.3% E243* (GAA→TAA)  lipA ← lipoate synthase
RA 671,701:1 +T 9.4% coding (303/978 nt) ybeQ ← Sel1 family TPR‑like repeat protein
RA 686,774 T→C 7.8% K156R (AAA→AGA)  ybeX ← putative ion transport
RA 709,273 C→A 5.3% T87N (ACT→AAT)  pgm → phosphoglucomutase
RA 727,171 T→G 5.5% N711K (AAT→AAG rhsC → Rhs family putative polymorphic toxin
RA 727,174 C→T 5.4% G712G (GGC→GGT rhsC → Rhs family putative polymorphic toxin
RA 727,207 C→T 5.8% R723R (CGC→CGT rhsC → Rhs family putative polymorphic toxin
RA 730,138 A→G 8.4% pseudogene (550/1521 nt) rhsO → pseudogene, Rhs family protein
RA 734,251 C→T 7.8% T235I (ACC→ATC)  ybfD → H repeat‑associated putative transposase
RA 752,091 G→T 5.4% P243P (CCG→CCT sdhA → succinate dehydrogenase, flavoprotein subunit
RA 763,877 C→A 5.1% F148L (TTC→TTA mngB → alpha‑mannosidase
RA 804,118 G→A 5.1% A199V (GCG→GTG)  bioA ← 7,8‑diaminopelargonic acid synthase, PLP‑dependent
RA 811,097 G→A 6.6% intergenic (+94/+98) uvrB → / ← ybhK excinulease of nucleotide excision repair, DNA damage recognition component/putative NAD(P)‑binding transferase
RA 824,963 C→A 8.3% D156Y (GAC→TAC)  ybhG ← putative membrane fusion protein (MFP) component of efflux pump, membrane anchor
RA 842,411 C→A 8.9% A55A (GCG→GCT glnP ← glutamine transporter subunit
RA 846,863 C→T 10.1% T397T (ACG→ACA ybiP ← putative EptAB family phosphoethanolamine transferase, inner membrane protein
RA 948,504 C→A 5.8% L169L (CTG→CTT pflB ← formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
RA 960,241 C→A 12.0% Q156K (CAG→AAG)  ycaI → ComEC family inner membrane protein
RA 967,316 C→A 9.7% Q37K (CAG→AAG)  ycbJ → protein kinase‑like domain protein
RA 971,447 C→A 7.4% L124M (CTG→ATG)  mukE → chromosome condensin MukBEF, MukE localization factor
RA 977,871 G→T 6.4% G457C (GGC→TGC)  ldtD → murein L,D‑transpeptidase
RA 1,004,487 C→T 5.3% T396T (ACC→ACT rlmL → fused 23S rRNA m(2)G2445 and m(7)G2069 methyltransferase, SAM‑dependent
RA 1,011,112 G→A 5.9% intergenic (+197/‑59) pqiC → / → rmf DUF330 family putative lipoprotein/ribosome modulation factor
RA 1,012,077 C→A 5.0% R560L (CGT→CTT)  ycbZ ← putative peptidase
RA 1,017,806 G→T 6.5% R512S (CGC→AGC)  yccS ← putative transporter, FUSC superfamily inner membrane protein, tandem domains
RA 1,068,323 G→A 10.7% A382V (GCA→GTA)  rutA ← pyrimidine oxygenase, FMN‑dependent
RA 1,110,322 G→A 7.4% R209W (CGG→TGG)  mdtG ← putative drug efflux system protein
RA 1,116,137 G→T 14.3% intergenic (‑95/+20) solA ← / ← bssS N‑methyltryptophan oxidase, FAD‑binding/biofilm regulator
RA 1,150,863 G→T 9.7% W95L (TGG→TTG)  tmk → thymidylate kinase
RA 1,160,479 C→A 20.3% S310* (TCG→TAG)  nagZ → beta N‑acetyl‑glucosaminidase
RA 1,171,990 C→A 8.1% L370M (CTG→ATG)  lolC → lipoprotein‑releasing system transmembrane protein
RA 1,173,681 C→A 6.9% I302I (ATC→ATA lolE → lipoprotein‑releasing system transmembrane protein
RA 1,175,865 G→T 10.2% intergenic (+50/+70) cobB → / ← ycfZ deacetylase of acs and cheY, chemotaxis regulator/inner membrane protein
RA 1,207,184 A→G 24.3% C92C (TGT→TGC iraM ← RpoS stabilzer during Mg starvation, anti‑RssB factor
RA 1,217,742 Δ1 bp 10.0% intergenic (+38/+19) ycgH → / ← ymgD pseudogene; putative ATP‑binding component of a transport system/uncharacterized protein
RA 1,217,748 T→C 10.8% intergenic (+44/+13) ycgH → / ← ymgD pseudogene; putative ATP‑binding component of a transport system/uncharacterized protein
RA 1,229,883 Δ1 bp 10.8% coding (291/1542 nt) nhaB ← sodium:proton antiporter
RA 1,239,080 G→T 9.5% G149W (GGG→TGG)  emtA → lytic murein endotransglycosylase E
RA 1,265,182 C→T 19.0% G29S (GGC→AGC)  ldrB ← toxic polypeptide, small
RA 1,266,708 C→A 6.0% E200* (GAG→TAG)  chaA ← calcium/sodium:proton antiporter
RA 1,282,243 G→A 6.1% intergenic (+261/+279) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,421 A→G 10.3% intergenic (+439/+101) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,430 C→T 10.4% intergenic (+448/+92) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,439 A→G 16.1% intergenic (+457/+83) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,495 T→G 5.1% intergenic (+513/+27) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,497 A→G 7.1% intergenic (+515/+25) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,299,223 C→T 5.9% G71G (GGC→GGT oppD → oligopeptide transporter subunit
RA 1,344,871 C→A 5.3% L142L (CTG→CTT fabI ← enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent
RA 1,345,451 G→T 7.5% intergenic (‑155/+213) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
RA 1,384,179 G→A 6.8% A226V (GCG→GTG)  mpaA ← murein peptide amidase A
RA 1,408,669 C→T 7.1% A128T (GCT→ACT)  recT ← Rac prophage; recombination and repair protein
RA 1,422,040 A→G 8.2% pseudogene (944/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,060 G→C 8.9% pseudogene (924/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,150 C→T 6.6% pseudogene (834/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,252 A→G 5.3% pseudogene (732/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,627 A→G 6.6% pseudogene (357/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,798 A→G 6.8% pseudogene (186/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,442,402 G→T 6.8% V209V (GTG→GTT feaB → phenylacetaldehyde dehydrogenase
RA 1,460,650 C→T 7.8% pseudogene (1002/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,819 G→A 5.7% pseudogene (1171/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,488,240 C→T 8.3% A505V (GCG→GTG)  trg → methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
RA 1,505,616 G→T 7.6% E453* (GAA→TAA)  ydcR → putative DNA‑binding transcriptional regulator/putative amino transferase
RA 1,508,281 C→A 5.7% S65* (TCG→TAG)  ydcU → putative spermidine/putrescine transporter subunit
RA 1,508,958 C→A 5.4% L291M (CTG→ATG)  ydcU → putative spermidine/putrescine transporter subunit
RA 1,535,331 C→T 13.4% W506* (TGG→TAG)  narZ ← nitrate reductase 2 (NRZ), alpha subunit
RA 1,535,660 C→A 12.0% W396C (TGG→TGT narZ ← nitrate reductase 2 (NRZ), alpha subunit
RA 1,536,926 C→T 12.1% intergenic (‑79/+3) narZ ← / ← narU nitrate reductase 2 (NRZ), alpha subunit/nitrate/nitrite transporter
RA 1,593,906 G→T 7.4% P187P (CCC→CCA lsrK ← autoinducer‑2 (AI‑2) kinase
RA 1,608,313 C→A 7.6% A216A (GCG→GCT sad ← succinate semialdehyde dehydrogenase, NAD(P)+‑dependent
RA 1,609,888 G→T 8.7% E276D (GAG→GAT yneJ → putative DNA‑binding transcriptional regulator
RA 1,624,979 C→T 8.4% Q64Q (CAG→CAA ydfI ← putative NAD‑dependent D‑mannonate oxidoreductase
RA 1,666,923 A→G 8.1% Q236R (CAA→CGA)  ydgD → putative peptidase
RA 1,680,042 C→T 5.3% L268L (CTG→CTA fumC ← fumarate hydratase (fumarase C),aerobic Class II
RA 1,680,118 G→A 7.4% A243V (GCG→GTG)  fumC ← fumarate hydratase (fumarase C),aerobic Class II
RA 1,698,243 T→A 6.4% D108V (GAT→GTT)  ydgJ ← putative oxidoreductase
RA 1,702,919 C→G 5.5% Q582E (CAA→GAA)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,702,966 T→A 10.0% R597R (CGT→CGA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,707,517 C→A 7.1% F164L (TTC→TTA dtpA → dipeptide and tripeptide permease A
RA 1,711,270 C→T 6.4% A70A (GCG→GCA tyrS ← tyrosyl‑tRNA synthetase
RA 1,713,443 G→T 7.3% A139A (GCC→GCA anmK ← anhydro‑N‑acetylmuramic acid kinase
RA 1,737,458 C→A 8.2% S14S (TCG→TCT ribC ← riboflavin synthase, alpha subunit
RA 1,740,742 T→G 8.4% noncoding (51/77 nt) valV → tRNA‑Val
RA 1,755,705 T→C 5.9% Q115R (CAG→CGG)  sufD ← component of SufBCD Fe‑S cluster assembly scaffold
RA 1,802,457 G→A 5.8% Q135Q (CAG→CAA yniA → fructosamine kinase family protein
RA 1,828,017 C→A 7.1% A120A (GCC→GCA ydjX → TVP38/TMEM64 family inner membrane protein
RA 1,834,750 G→A 5.4% A343T (GCT→ACT)  ynjE → molybdopterin synthase sulfurtransferase
RA 1,868,362 G→T 10.4% S26S (TCC→TCA yoaI ← uncharacterized protein
RA 1,889,829 G→A 5.9% S256S (TCG→TCA pabB → aminodeoxychorismate synthase, subunit I
RA 1,894,931 C→A 5.7% M304I (ATG→ATT yoaE ← putative membrane protein/conserved protein
RA 1,912,336 C→T 8.5% Y190Y (TAC→TAT yebT → MCE domain protein
RA 1,915,438 C→A 12.9% P320H (CCC→CAC)  rsmF → 16S rRNA m(5)C1407 methyltransferase, SAM‑dependent
RA 1,922,551 G→T 7.8% D182E (GAC→GAA ptrB ← protease II
RA 1,923,915 G→T 6.8% S17Y (TCT→TAT)  yebE ← DUF533 family inner membrane protein
RA 1,928,323 G→T 5.6% P180Q (CCG→CAG)  edd ← 6‑phosphogluconate dehydratase
RA 1,930,951 A→G 13.7% S15G (AGC→GGC)  yebK → putative DNA‑binding transcriptional regulator
RA 1,953,362 C→A 5.5% V54V (GTG→GTT cutC ← copper homeostasis protein
RA 1,988,343 C→A 7.9% K166N (AAG→AAT uvrY ← response regulator in two‑component regulatory system with BarA
RA 1,991,903 C→A 7.1% G20W (GGG→TGG)  yecS ← ABC family putative inner membrane permease
RA 1,997,988 C→T 5.1% S212S (AGC→AGT fliD → flagellar filament capping protein
RA 2,056,824 G→A 10.0% intergenic (‑20/+45) ldtA ← / ← cobT L,D‑transpeptidase linking Lpp to murein/nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase
RA 2,059,656 T→C 11.3% intergenic (‑21/+130) yeeH ← / ← insH1 pseudogene/IS5 transposase and trans‑activator
RA 2,082,293 C→A 5.5% R106L (CGC→CTC)  yeeZ ← putative epimerase
RA 2,144,397 C→A 6.4% G24C (GGT→TGT)  yegI ← protein kinase‑related putative non‑specific DNA‑binding protein
RA 2,151,727 C→A 6.7% P995Q (CCG→CAG)  mdtB → multidrug efflux system, subunit B
RA 2,176,392 C→T 7.5% G247S (GGT→AGT)  yegX ← putative family 25 glycosyl hydrolase
RA 2,201,626 G→T 5.8% G61* (GGA→TGA)  yehP → VMA domain putative YehL ATPase stimulator
RA 2,207,163 G→T 7.0% R321R (CGG→AGG)  yehU ← inner membrane putative sensory kinase in two‑component system with YehT
RA 2,209,069 G→A 5.4% G242D (GGC→GAC)  mlrA → transcriptional activator of csgD and csgBA
RA 2,219,202 C→A 7.9% intergenic (‑92/‑78) yohC ← / → yohD Yip1 family inner membrane protein/DedA family inner membrane protein
RA 2,238,440 G→T 6.5% S603R (AGC→AGA cirA ← colicin IA outer membrane receptor and translocator; ferric iron‑catecholate transporter
RA 2,244,733 G→T 7.4% G139C (GGT→TGT)  nfo → endonuclease IV with intrinsic 3'‑5' exonuclease activity
RA 2,298,467 T→C 6.5% intergenic (+595/+120) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,492 A→G 8.6% intergenic (+620/+95) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,312,614 C→A 9.1% D248Y (GAT→TAT)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
RA 2,362,168 G→A 5.5% L217L (CTG→CTA arnA → fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase
RA 2,383,997 G→T 5.5% L330M (CTG→ATG)  nuoN ← NADH:ubiquinone oxidoreductase, membrane subunit N
RA 2,442,618 G→A 5.4% L178L (CTG→CTA smrB → putative DNA endonuclease
RA 2,443,767 G→A 6.2% S101S (AGC→AGT yfcP ← putative fimbrial‑like adhesin protein
RA 2,447,704 C→A 6.8% pseudogene (777/2646 nt) yfcU ← pseudogene, FimD fimbrial export usher family;putative membrane; Not classified; putative outer membrane protein
RA 2,452,455 G→A 7.7% P62S (CCG→TCG)  fadJ ← fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
RA 2,458,408 C→A 7.2% G27W (GGG→TGG)  mlaA ← ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component
RA 2,469,757 C→T 6.2% intergenic (+47/‑32) ypdJ → / → torI exisonase remnant/response regulator inhibitor for tor operon
RA 2,519,176 C→A 5.1% R231R (CGA→AGA)  yfeN → putative outer membrane protein
RA 2,522,619 G→T 6.0% A306D (GCC→GAC)  ligA ← DNA ligase, NAD(+)‑dependent
RA 2,567,493 C→A 7.0% D58Y (GAT→TAT)  eutT ← cobalamin adenosyltransferase involved in ethanolamine utilization
RA 2,570,400 A→G 6.3% V446A (GTT→GCT)  maeB ← malic enzyme: putative oxidoreductase/putative phosphotransacetylase
RA 2,574,466 G→T 5.1% R491L (CGA→CTA)  tktB → transketolase 2, thiamine triphosphate‑binding
RA 2,575,692 A→G 5.6% L149L (TTG→CTG)  ypfG ← DUF1176 family protein
RA 2,597,458 G→T 5.9% V85L (GTG→TTG)  hyfC → hydrogenase 4, membrane subunit
RA 2,602,399 C→A 14.0% P185H (CCT→CAT)  hyfG → hydrogenase 4, subunit
RA 2,608,165 C→A 5.1% intergenic (+24/+14) focB → / ← yfgO putative formate transporter/putative UPF0118 family inner membrane permease
RA 2,631,105 C→A 5.3% D303Y (GAC→TAC)  bamB ← BamABCDE complex OM biogenesis lipoprotein
RA 2,647,794 G→A 6.1% P167S (CCG→TCG)  sseB ← rhodanase‑like enzyme, sulfur transfer from thiosulfate
RA 2,698,804 A→C 7.2% R560R (CGT→CGG lepA ← back‑translocating elongation factor EF4, GTPase
RA 2,729,190 C→A 8.7% G61C (GGT→TGT)  rluD ← 23S rRNA pseudouridine(1911,1915,1917) synthase
RA 2,763,992 A→C 9.2% G63G (GGA→GGC ypjK → CP4‑57 prophage; putative inner membrane protein
RA 2,771,592 T→A 8.1% L1498F (TTA→TTT ypjA ← adhesin‑like autotransporter
RA 2,771,620 C→G 9.5% G1489A (GGT→GCT)  ypjA ← adhesin‑like autotransporter
RA 2,791,802 G→T 6.5% A18D (GCT→GAT)  stpA ← DNA binding protein, nucleoid‑associated
RA 2,811,350 A→C 8.7% intergenic (‑131/+68) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,811,405 A→G 5.4% intergenic (‑186/+13) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,811,408 T→A 6.3% intergenic (‑189/+10) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,811,765 C→A 6.8% intergenic (‑132/+67) argY ← / ← argV tRNA‑Arg/tRNA‑Arg
RA 2,811,771 G→A 5.7% intergenic (‑138/+61) argY ← / ← argV tRNA‑Arg/tRNA‑Arg
RA 2,833,521 C→A 5.7% T213T (ACC→ACA ascF → fused cellobiose/arbutin/salicin‑specific PTS enzymes: IIB component/IC component
RA 2,840,917 G→T 9.3% P562T (CCG→ACG)  hycC ← hydrogenase 3, membrane subunit
RA 2,887,290 C→A 6.4% R5S (CGT→AGT)  ygcO → putative 4Fe‑4S cluster‑containing protein
RA 2,898,511 C→A 5.9% V89V (GTG→GTT queE ← 7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
RA 2,900,421 C→A 14.3% D294Y (GAC→TAC)  eno ← enolase
RA 2,903,090 G→T 12.6% intergenic (‑65/+163) pyrG ← / ← mazG CTP synthetase/nucleoside triphosphate pyrophosphohydrolase
RA 2,933,546 G→T 7.9% R353S (CGC→AGC)  rlmM ← 23S rRNA C2498 2'‑O‑ribose methyltransferase, SAM‑dependent
RA 2,959,130 C→A 23.1% P89P (CCG→CCT lgt ← phosphatidylglycerol‑prolipoprotein diacylglyceryl transferase
RA 2,979,475 C→A 5.0% T90T (ACC→ACA yqeG → putative transporter
RA 3,030,463 C→A 8.3% G335* (GGA→TGA)  recJ ← ssDNA exonuclease, 5' ‑‑> 3'‑specific
RA 3,046,396 G→T 6.5% A161D (GCT→GAT)  ubiH ← 2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding
RA 3,053,249 C→A 5.3% F46L (TTC→TTA argP → transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
RA 3,064,718 T→G 9.8% intergenic (‑115/+100) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,072,719 C→T 12.1% intergenic (‑30/+284) cmtB ← / ← tktA putative mannitol‑specific enzyme IIA component of PTS/transketolase 1, thiamine triphosphate‑binding
RA 3,073,770 C→A 9.0% V409F (GTT→TTT)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,076,165 G→T 6.7% intergenic (+135/+71) loiP → / ← speB Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; heat shock protein that binds Era/agmatinase
RA 3,086,485 G→T 6.2% E64* (GAA→TAA)  yqgE → uncharacterized protein
RA 3,127,539 C→T 11.8% S17L (TCA→TTA)  yghT → putative ATP‑binding protein
RA 3,138,358 C→T 6.9% V48I (GTA→ATA)  hybA ← hydrogenase 2 4Fe‑4S ferredoxin‑type component
RA 3,162,604 G→T 5.7% intergenic (‑14/+39) ygiV ← / ← ygiW transcriptional repressor for mcbR biofilm gene/hydrogen peroxide and cadmium resistance periplasmic protein; stress‑induced OB‑fold protein
RA 3,183,528 G→T 10.2% E97* (GAA→TAA)  yqiH → putative periplasmic pilin chaperone
RA 3,184,639 C→A 5.0% L217I (CTT→ATT)  yqiI → fimbrial protein
RA 3,216,303 C→A 9.5% V104V (GTC→GTA ebgA → evolved beta‑D‑galactosidase, alpha subunit
RA 3,223,587 C→A 7.1% D447E (GAC→GAA ygjK → alpha‑glucosidase
RA 3,238,257 C→A 8.6% intergenic (‑157/‑206) uxaC ← / → exuT uronate isomerase/hexuronate transporter
RA 3,259,592 G→T 19.7% S278* (TCG→TAG)  tdcA ← tdc operon transcriptional activator
RA 3,308,212 C→A 5.1% E388* (GAA→TAA)  infB ← translation initiation factor IF‑2
RA 3,337,273 C→A 8.6% P179T (CCG→ACG)  lptA → periplasmic LPS‑binding protein
RA 3,340,106 G→A 6.3% R57Q (CGG→CAG)  ptsN → sugar‑specific enzyme IIA component of PTS
RA 3,349,655 G→A 5.4% A524A (GCG→GCA gltB → glutamate synthase, large subunit
RA 3,351,193 C→A 8.6% S1037* (TCG→TAG)  gltB → glutamate synthase, large subunit
RA 3,352,440 T→C 6.4% F1453L (TTC→CTC)  gltB → glutamate synthase, large subunit
RA 3,378,464 C→A 11.1% P135T (CCT→ACT)  argR → l‑arginine‑responsive arginine metabolism regulon transcriptional regulator
RA 3,411,742 T→G 16.7% intergenic (+246/‑7) acrF → / → yhdV multidrug efflux system protein/putative outer membrane protein
RA 3,416,708 G→A 9.2% intergenic (+155/+74) yhdZ → / ← rrfF putative amino‑acid transporter subunit/5S ribosomal RNA of rrnD operon
RA 3,416,715 T→C 8.4% intergenic (+162/+67) yhdZ → / ← rrfF putative amino‑acid transporter subunit/5S ribosomal RNA of rrnD operon
RA 3,505,968 A→C 7.1% intergenic (‑265/+25) yhfZ ← / ← trpS putative DNA‑binding transcriptional regulator/tryptophanyl‑tRNA synthetase
RA 3,512,041:1 +G 5.8% coding (383/522 nt) aroK ← shikimate kinase I
RA 3,512,041:2 +T 5.8% coding (383/522 nt) aroK ← shikimate kinase I
RA 3,521,752 G→T 5.8% G642V (GGC→GTC)  yrfF → inner membrane protein
RA 3,551,421 C→A 10.9% L6L (CTG→CTT rtcB ← RNA‑splicing ligase
RA 3,553,124 C→T 7.9% Q500* (CAG→TAG)  rtcR → sigma 54‑dependent transcriptional regulator of rtcBA expression
RA 3,568,450 C→A 5.3% A7E (GCA→GAA)  yhgN → UPF0056 family inner membrane protein
RA 3,581,404 G→T 7.4% L24M (CTG→ATG)  ugpQ ← glycerophosphodiester phosphodiesterase, cytosolic
RA 3,601,093 G→A 5.7% G428D (GGT→GAT)  zntA → zinc, cobalt and lead efflux system
RA 3,605,087 C→A 6.8% E3* (GAA→TAA)  yhhS ← putative arabinose efflux transporter
RA 3,606,516 C→A 8.8% P63Q (CCG→CAG)  acpT → 4'‑phosphopantetheinyl transferase
RA 3,609,197 G→T 6.7% M199I (ATG→ATT nikB → nickel transporter subunit
RA 3,660,722 A→G 9.1% H73H (CAT→CAC gadA ← glutamate decarboxylase A, PLP‑dependent
RA 3,660,728 G→A 9.0% N71N (AAC→AAT gadA ← glutamate decarboxylase A, PLP‑dependent
RA 3,662,961 C→A 6.8% Q4K (CAG→AAG)  treF → cytoplasmic trehalase
RA 3,707,729 G→T 5.9% Q676K (CAG→AAG)  bisC ← biotin sulfoxide reductase
RA 3,719,163 G→T 6.1% intergenic (‑91/‑84) ysaB ← / → wecH uncharacterized protein/O‑acetyltransferase for enterobacterial common antigen (ECA)
RA 3,720,359 C→A 6.1% V121V (GTG→GTT yiaA ← YiaAB family inner membrane protein, tandem domains
RA 3,725,795 G→T 7.4% E79* (GAA→TAA)  xylG → fused D‑xylose transporter subunits of ABC superfamily: ATP‑binding components
RA 3,728,102 G→A 6.1% V341V (GTG→GTA xylH → D‑xylose ABC transporter permease subunit
RA 3,758,716 A→G 6.4% R1058R (AGA→AGG rhsA → Rhs family protein, putative polymorphic toxin; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
RA 3,807,911 G→A 5.6% A20T (GCG→ACG)  slmA → nucleoid occlusion factor, anti‑FtsZ division inhibitor
RA 3,849,637 C→A 8.5% D11Y (GAT→TAT)  yidH ← DUF202 family inner membrane protein
RA 3,850,601 G→A 7.9% D388D (GAC→GAT yidJ ← sulfatase/phosphatase superfamily protein
RA 3,883,873 T→C 12.5% V93A (GTT→GCT)  tnaB → tryptophan transporter of low affinity
RA 3,883,876 C→T 7.0% A94V (GCC→GTC)  tnaB → tryptophan transporter of low affinity
RA 3,889,822 G→T 7.1% P50T (CCG→ACG)  purP ← adenine permease, high affinity; adenine:H+ symporter
RA 3,897,978 G→T 7.8% P327Q (CCG→CAG)  bglF ← fused beta‑glucoside‑specific PTS enzymes: IIA component/IIB component/IIC component
RA 3,898,025 C→T 5.8% P311P (CCG→CCA bglF ← fused beta‑glucoside‑specific PTS enzymes: IIA component/IIB component/IIC component
RA 3,922,048 C→A 5.9% E306* (GAA→TAA)  viaA ← stimulator of RavA ATPase activity; von Willebrand factor domain protein
RA 3,955,206 G→T 6.0% L390L (CTG→CTT rep → DNA helicase and single‑stranded DNA‑dependent ATPase
RA 3,973,769 C→A 5.3% L152M (CTG→ATG)  wecG → UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase
RA 3,988,611 G→T 7.8% V164F (GTC→TTC)  dapF → diaminopimelate epimerase
RA 3,993,739 C→A 6.7% T226T (ACG→ACT yigE ← DUF2233 family protein
RA 4,008,923 G→T 10.4% Q203K (CAG→AAG)  ysgA ← putative carboxymethylenebutenolidase
RA 4,049,086 C→A 5.7% R205L (CGC→CTC)  glnL ← sensory histidine kinase in two‑component regulatory system with GlnG
RA 4,106,240 T→C 5.8% E304G (GAA→GGA)  glpK ← glycerol kinase
RA 4,113,302 G→A 5.9% intergenic (‑35/+57) ftsN ← / ← cytR essential cell division protein/Anti‑activator for CytR‑CRP nucleoside utilization regulon
RA 4,167,536 G→T 6.4% W84L (TGG→TTG)  secE → preprotein translocase membrane subunit
RA 4,173,745 G→A 11.9% G858D (GGT→GAT)  rpoB → RNA polymerase, beta subunit
RA 4,178,208 C→T 17.2% S977S (TCC→TCT rpoC → RNA polymerase, beta prime subunit
RA 4,178,209 C→A 24.7% R978S (CGT→AGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,178,500 C→T 6.7% R1075C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,178,870 T→A 7.3% V1198E (GTG→GAG)  rpoC → RNA polymerase, beta prime subunit
RA 4,207,571 G→T 8.2% G179C (GGT→TGT)  aceA → isocitrate lyase
RA 4,218,686 G→T 5.3% G343V (GGC→GTC)  yjbB → putative Na+/Pi‑cotransporter
RA 4,270,774 G→A 9.5% L289L (CTG→CTA yjcE → putative cation/proton antiporter
RA 4,298,328 A→G 5.6% L320P (CTT→CCT)  alsC ← D‑allose transporter subunit
RA 4,299,213 T→C 11.1% K25R (AAA→AGA)  alsC ← D‑allose transporter subunit
RA 4,301,540 C→A 14.0% G107V (GGC→GTC)  alsB ← D‑allose transporter subunit
RA 4,349,803 C→T 6.7% V16I (GTT→ATT)  cadB ← putative lysine/cadaverine transporter
RA 4,382,813 C→A 6.9% E358* (GAA→TAA)  queG ← epoxyqueuosine reductase, cobalamine‑stimulated; queosine biosynthesis
RA 4,397,211 G→A 5.9% G247G (GGG→GGA rnr → exoribonuclease R, RNase R
RA 4,399,784 C→A 10.6% T231T (ACC→ACA rlmB → 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
RA 4,424,794 G→T 7.5% P530Q (CCG→CAG)  cpdB ← 2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase
RA 4,434,531 G→A 5.3% P201P (CCG→CCA tamB → translocation and assembly module for autotransporter export, inner membrane subunit
RA 4,443,766 G→T 16.3% G123W (GGG→TGG)  yjfF → inner membrane putative ABC superfamily sugar transporter permease
RA 4,450,603 C→A 6.6% W625C (TGG→TGT nrdD ← anaerobic ribonucleoside‑triphosphate reductase
RA 4,451,946 T→C 6.0% I178V (ATC→GTC)  nrdD ← anaerobic ribonucleoside‑triphosphate reductase
RA 4,455,789 G→T 7.4% T70N (ACC→AAC)  treB ← fused trehalose(maltose)‑specific PTS enzyme: IIB component/IIC component
RA 4,457,232 A→G 6.1% intergenic (‑169/‑16) treR ← / → mgtL trehalose 6‑phosphate‑inducible trehalose regulon transcriptional repressor/regulatory leader peptide for mgtA
RA 4,498,906 T→C 6.7% pseudogene (81/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,498,909 G→T 6.9% pseudogene (84/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,498,981 G→A 5.2% pseudogene (156/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,499,835 A→G 5.1% pseudogene (215/330 nt) yjhV → pseudogene, KpLE2 phage‑like element
RA 4,501,356 G→T 5.1% P292P (CCC→CCA fecD ← iron‑dicitrate transporter subunit
RA 4,508,444 C→A 5.5% P34H (CCT→CAT)  insA → IS1 repressor TnpA
RA 4,508,733 A→C 5.1% pseudogene (196/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,740 G→A 5.3% pseudogene (203/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,897 T→A 6.9% pseudogene (360/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,900 C→T 5.2% pseudogene (363/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,604,320 T→C 9.9% intergenic (+243/+177) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,605,001 G→A 6.1% G120G (GGC→GGT yjjW ← putative pyruvate formate lyase activating enzyme
RA 4,605,715 C→A 6.5% G390C (GGC→TGC)  yjjI ← DUF3029 family protein, putative glycine radical enzyme
RA 4,620,909 C→A 6.6% A120A (GCC→GCA slt → lytic murein transglycosylase, soluble

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =73 (1.070)47 (0.690) 28/250 NT 39.2% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 370378 =NA (NA)15 (0.230) 4/234 NT NA intergenic (+41/‑537) mhpE/mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
?CP009273 372785 = NA (NA)intergenic (+18/+206) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 1203246 =32 (0.470)33 (0.550) 19/218 NT 48.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 41 (0.690)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326132 (0.470)25 (0.420) 13/218 NT 42.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 41 (0.690)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related