breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 30,387 C→T 8.4% A246V (GCC→GTC)  carA → carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
RA 65,142 G→T 5.9% A213A (GCC→GCA polB ← DNA polymerase II
RA 90,927 C→A 5.4% I425I (ATC→ATA murE → UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate:meso‑ diaminopimelate ligase
RA 200,626 G→T 6.6% A264A (GCG→GCT lpxB → tetraacyldisaccharide‑1‑P synthase
RA 221,173 T→G 6.4% noncoding (916/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 235,640 C→T 7.0% pseudogene (48/273 nt) yafF → pseudogene, H repeat‑associated protein
RA 247,164 C→T 5.2% pseudogene (606/756 nt) lafU → pseudogene, lateral flagellar motor protein fragment
RA 249,531 C→T 11.4% S112S (AGC→AGT yafP → putative acyl‑CoA transferase
RA 305,573 G→T 8.5% L55L (CTC→CTA ecpB ← ECP production pilus chaperone
RA 390,847 C→A 6.1% M299I (ATG→ATT ampH ← D‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase; penicillin‑binding protein; weak beta‑lactamase
RA 404,893 C→A 6.1% A195S (GCC→TCC)  rdgC ← nucleoid‑associated ssDNA and dsDNA binding protein; competitive inhibitor of RecA function
RA 472,858 C→A 8.6% A406S (GCC→TCC)  ylaB ← putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 476,240 C→A 6.9% intergenic (‑76/+470) tomB ← / ← acrB Hha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein
RA 497,707 Δ1 bp 8.3% coding (988/1677 nt) ybaL ← inner membrane putative NAD(P)‑binding transporter
RA 499,294 C→A 6.2% G287W (GGG→TGG)  fsr ← putative fosmidomycin efflux system protein
RA 516,006 G→A 5.2% Q45Q (CAG→CAA ybbP → putative ABC transporter permease
RA 516,173 C→T 6.2% A101V (GCG→GTG)  ybbP → putative ABC transporter permease
RA 576,565 G→A 10.0% A92A (GCG→GCA nohD → DLP12 prophage; DNA packaging protein
RA 578,457 C→A 8.4% pseudogene (49/183 nt) tfaX → pseudogene, DLP12 prophage;Phage or Prophage Related
RA 589,708 G→T 5.2% I173I (ATC→ATA cusS ← sensory histidine kinase in two‑component regulatory system with CusR, senses copper ions
RA 682,808 C→A 13.7% V132V (GTG→GTT gltI ← glutamate, aspartate binding protein, periplasmic; part of GltJKLI ABC transporter
RA 727,989 C→A 6.6% A984E (GCA→GAA)  rhsC → Rhs family putative polymorphic toxin
RA 730,083 G→A 13.0% pseudogene (495/1521 nt) rhsO → pseudogene, Rhs family protein
RA 731,020 C→A 5.9% pseudogene (1432/1521 nt) rhsO → pseudogene, Rhs family protein
RA 757,509 C→T 7.1% R178C (CGT→TGT)  sucB → dihydrolipoyltranssuccinase
RA 811,085 T→C 7.5% intergenic (+82/+110) uvrB → / ← ybhK excinulease of nucleotide excision repair, DNA damage recognition component/putative NAD(P)‑binding transferase
RA 811,094 T→G 10.2% intergenic (+91/+101) uvrB → / ← ybhK excinulease of nucleotide excision repair, DNA damage recognition component/putative NAD(P)‑binding transferase
RA 811,095 G→A 8.2% intergenic (+92/+100) uvrB → / ← ybhK excinulease of nucleotide excision repair, DNA damage recognition component/putative NAD(P)‑binding transferase
RA 811,096 C→T 8.8% intergenic (+93/+99) uvrB → / ← ybhK excinulease of nucleotide excision repair, DNA damage recognition component/putative NAD(P)‑binding transferase
RA 811,097 G→A 14.9% intergenic (+94/+98) uvrB → / ← ybhK excinulease of nucleotide excision repair, DNA damage recognition component/putative NAD(P)‑binding transferase
RA 811,099:1 +A 8.0% intergenic (+96/+96) uvrB → / ← ybhK excinulease of nucleotide excision repair, DNA damage recognition component/putative NAD(P)‑binding transferase
RA 832,455 C→A 6.7% P217T (CCG→ACG)  ybiC → putative dehydrogenase
RA 875,194 C→A 7.7% R333S (CGT→AGT)  yliI → soluble aldose sugar dehydrogenase
RA 894,952 C→A 5.7% R327S (CGT→AGT)  rlmC → 23S rRNA m(5)U747 methyltransferase, SAM‑dependent
RA 955,928 C→A 9.5% S70Y (TCC→TAC)  ycaL → putative peptidase‑related chaperone
RA 968,382 A→G 8.9% L159P (CTG→CCG)  ycbC ← DUF218 superfamily protein
RA 1,015,272 C→A 8.3% G80C (GGT→TGT)  ompA ← outer membrane protein A (3a;II*;G;d)
RA 1,020,310 G→A 6.4% V128V (GTG→GTA helD → DNA helicase IV
RA 1,024,891 G→A 5.6% P179S (CCA→TCA)  rlmI ← 23S rRNA m(5)C1962 methyltransferase, SAM‑dependent
RA 1,026,023 Δ1 bp 5.6% coding (102/330 nt) tusE ← mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
RA 1,036,640 C→T 6.4% R190W (CGG→TGG)  appA → phosphoanhydride phosphorylase
RA 1,107,318 C→A 9.8% R334S (CGC→AGC)  opgH → membrane glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist
RA 1,150,703 G→A 5.8% G42S (GGC→AGC)  tmk → thymidylate kinase
RA 1,158,400 C→A 6.2% L103M (CTG→ATG)  lpoB → OM lipoprotein stimulator of MrcB transpeptidase
RA 1,161,072 G→A 5.4% E158E (GAG→GAA ycfP → putative UPF0227 family esterase
RA 1,196,044 C→A 6.1% R73S (AGG→AGT intE ← e14 prophage; putative integrase
RA 1,205,973 C→A 12.2% Q58K (CAA→AAA)  mcrA → putative 5‑methylcytosine/5‑hydroxymethylcytosine‑specific restriction nuclease; 5‑methylcytosine DNA binding protein; e14 prophage gene
RA 1,239,619 G→T 8.0% P122Q (CCA→CAA)  ycgR ← flagellar velocity braking protein, c‑di‑GMP‑regulated
RA 1,275,217 G→T 7.7% intergenic (+413/‑103) narK → / → narG nitrate/nitrite transporter/nitrate reductase 1, alpha subunit
RA 1,297,709 A→T 9.8% Y185F (TAT→TTT)  oppB → oligopeptide transporter subunit
RA 1,298,848 G→A 7.4% S253N (AGC→AAC)  oppC → oligopeptide transporter subunit
RA 1,381,352 G→A 12.1% A126T (GCA→ACA)  tyrR → aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase
RA 1,383,810 G→T 7.9% Q208H (CAG→CAT ycjG → L‑Ala‑D/L‑Glu epimerase
RA 1,386,463 C→A 9.1% G72G (GGC→GGA pgrR → murein peptide degradation regulator
RA 1,417,567 T→C 12.2% pseudogene (110/444 nt) rzpR → pseudogene, Rac prophage; Bacteriophage Rz lysis protein family;Phage or Prophage Related; putative Rac prophage endopeptidase; completely contained in another CDS
RA 1,450,956 G→T 7.5% Q90H (CAG→CAT paaE → ring 1,2‑phenylacetyl‑CoA epoxidase, NAD(P)H oxidoreductase component
RA 1,460,567 A→G 8.5% pseudogene (919/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,593 C→G 9.5% pseudogene (945/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,602 T→C 13.8% pseudogene (954/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,752 C→T 11.1% pseudogene (1104/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,761 A→G 10.8% pseudogene (1113/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,887 G→C 15.8% pseudogene (1239/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,896 C→T 17.6% pseudogene (1248/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,479,744 C→A 13.2% S809S (TCC→TCA hrpA → putative ATP‑dependent helicase
RA 1,526,840 G→A 7.1% G256G (GGG→GGA ydcC → H repeat‑associated putative transposase
RA 1,526,876 C→A 9.8% S268S (TCC→TCA ydcC → H repeat‑associated putative transposase
RA 1,527,189 A→G 10.4% T373A (ACG→GCG)  ydcC → H repeat‑associated putative transposase
RA 1,544,169 A→C 6.9% I838L (ATC→CTC)  fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
RA 1,544,172 A→G 7.1% N839D (AAC→GAC) ‡ fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
RA 1,544,174 C→T 7.2% N839N (AAC→AAT) ‡ fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
RA 1,544,177 A→G 7.3% K840K (AAA→AAG fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
RA 1,558,554 G→A 5.1% S479S (AGC→AGT dosP ← oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator
RA 1,566,159 G→A 17.9% D48D (GAC→GAT gadB ← glutamate decarboxylase B, PLP‑dependent
RA 1,629,745 T→A 18.5% A120A (GCA→GCT stfQ ← Qin prophage; putative side tail fiber assembly protein
RA 1,656,947 C→A 6.4% P712Q (CCA→CAA)  ynfF → S‑ and N‑oxide reductase, A subunit, periplasmic
RA 1,702,047 C→A 10.4% P291Q (CCG→CAG)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,702,963 C→A 7.2% A596A (GCC→GCA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,702,975 G→A 7.2% A600A (GCG→GCA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,703,007 A→T 6.3% E611V (GAA→GTA)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,703,101 C→G 7.5% A642A (GCC→GCG rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,703,104 G→A 5.1% E643E (GAG→GAA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,784,418 G→T 14.5% R362S (CGC→AGC)  ydiU ← UPF0061 family protein
RA 1,857,199 G→A 10.0% V58I (GTT→ATT)  gapA → glyceraldehyde‑3‑phosphate dehydrogenase A
RA 1,928,749 G→T 6.6% S38* (TCG→TAG)  edd ← 6‑phosphogluconate dehydratase
RA 1,965,923 C→T 6.2% V342V (GTG→GTA tar ← methyl‑accepting chemotaxis protein II
RA 2,007,584 C→T 6.7% S292L (TCA→TTA)  fliF → flagellar basal‑body MS‑ring and collar protein
RA 2,027,811 G→A 7.5% pseudogene (280/663 nt) yedS → pseudogene, outer membrane protein homology; putative outer membrane protein
RA 2,102,959 G→T 10.3% T36K (ACA→AAA)  wzxB ← putative polisoprenol‑linked O‑antigen transporter
RA 2,170,724 G→T 35.0% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,217,693:1 +G 5.4% coding (657/933 nt) pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,217,698 Δ1 bp 5.9% coding (652/933 nt) pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,217,699 A→C 5.9% N217K (AAT→AAG) ‡ pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,217,701 T→A 5.6% N217Y (AAT→TAT) ‡ pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,217,702 A→C 5.6% T216T (ACT→ACG) ‡ pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,217,703 G→A 5.6% T216I (ACT→ATT) ‡ pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,217,704 T→C 5.5% T216A (ACT→GCT) ‡ pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,217,707 T→G 5.5% N215H (AAT→CAT)  pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,217,708:1 +A 5.6% coding (642/933 nt) pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,217,709:1 +A 5.6% coding (641/933 nt) pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,217,709:2 +T 5.6% coding (641/933 nt) pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,217,710 G→T 5.5% R214S (CGC→AGC)  pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,217,711 G→C 5.5% F213L (TTC→TTG) ‡ pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,217,712 A→G 5.3% F213S (TTC→TCC) ‡ pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,217,713 A→T 5.3% F213I (TTC→ATC) ‡ pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,219,613 C→T 5.8% L112L (CTG→TTG)  yohD → DedA family inner membrane protein
RA 2,304,935 C→T 8.7% A27T (GCC→ACC)  apbE ← putative thiamine‑synthetic flavin transferase lipoprotein
RA 2,324,402 G→T 5.0% pseudogene (3480/4605 nt) yfaS ← pseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
RA 2,327,515 C→A 7.8% pseudogene (367/4605 nt) yfaS ← pseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
RA 2,333,809 Δ1 bp 5.1% intergenic (+41/+87) ubiG → / ← yfaL bifunctional 3‑demethylubiquinone‑9 3‑methyltransferase/ 2‑octaprenyl‑6‑hydroxy phenol methylase/adhesin
RA 2,378,895 C→T 7.3% A103T (GCT→ACT)  yfbK ← Von Willebrand factor domain putative lipoprotein
RA 2,422,584 C→A 10.8% Q378H (CAG→CAT purF ← amidophosphoribosyltransferase
RA 2,430,099 C→A 6.3% L10L (CTG→CTT usg ← putative semialdehyde dehydrogenase
RA 2,528,734 Δ1 bp 5.9% coding (1310/1728 nt) ptsI → PEP‑protein phosphotransferase of PTS system (enzyme I)
RA 2,589,632 C→A 5.3% A155A (GCG→GCT ypfJ ← putative neutral zinc metallopeptidase
RA 2,629,456 C→A 8.4% V420V (GTG→GTT der ← GTPase; multicopy suppressor of ftsJ
RA 2,633,390 A→G 7.7% V182A (GTG→GCG)  hisS ← histidyl tRNA synthetase
RA 2,690,483 G→T 5.1% E442* (GAA→TAA)  mltF → membrane‑bound lytic transglycosylase F, murein hydrolase
RA 2,719,426 T→C 8.2% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,724,437 G→T 5.8% noncoding (80/1542 nt) rrsG ← 16S ribosomal RNA of rrnG operon
RA 2,776,112 C→A 5.6% intergenic (‑27/+312) ypjA ← / ← pinH adhesin‑like autotransporter/pseudogene, invertase resolvase family
RA 2,798,587 C→A 5.2% A138A (GCC→GCA proV → glycine betaine transporter subunit
RA 2,815,353 A→G 13.3% A6A (GCT→GCC alaS ← alanyl‑tRNA synthetase
RA 2,824,415 G→T 8.4% H412N (CAT→AAT)  norR ← anaerobic nitric oxide reductase DNA‑binding transcriptional activator
RA 2,887,576 C→A 5.3% R8S (CGC→AGC)  ygcP → putative antiterminator regulatory protein
RA 2,895,606 G→T 8.5% D118Y (GAC→TAC)  ygcE → putative kinase
RA 2,956,559 G→A 5.1% Q173* (CAA→TAA)  ppdB ← putative prepilin peptidase‑dependent protein
RA 2,973,589 C→A 8.2% G136W (GGG→TGG)  ygeA ← Asp/Glu_racemase family protein
RA 3,015,569 C→T 8.2% P299S (CCG→TCG)  xdhD → putative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding
RA 3,107,435 C→A 5.8% D93Y (GAC→TAC)  pppA ← bifunctional prepilin leader peptidase/ methylase
RA 3,109,205:1 +T 5.7% coding (3267/4563 nt) yghJ ← DUF4092 family putative lipoprotein peptidase
RA 3,109,206 C→G 5.7% W1089S (TGG→TCG)  yghJ ← DUF4092 family putative lipoprotein peptidase
RA 3,198,656 A→G 6.4% K202E (AAG→GAG)  plsY → putative glycerol‑3‑phosphate acyltransferase
RA 3,209,603 G→T 5.0% P83Q (CCA→CAA)  yqjH ← putative siderophore interacting protein
RA 3,233,343 C→A 5.3% A14D (GCT→GAT)  sstT → sodium:serine/threonine symporter
RA 3,286,375 C→T 6.0% R107H (CGT→CAT)  rsmI ← 16S rRNA C1402 2'‑O‑ribose methyltransferase, SAM‑dependent
RA 3,319,721 C→T 8.9% G192S (GGT→AGT)  ftsH ← protease, ATP‑dependent zinc‑metallo
RA 3,340,030 C→A 5.1% L32M (CTG→ATG)  ptsN → sugar‑specific enzyme IIA component of PTS
RA 3,416,890 T→G 8.0% noncoding (12/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,433,794 C→A 36.8% D199Y (GAC→TAC)  rpoA ← RNA polymerase, alpha subunit
RA 3,433,808 T→G 14.9% Q194P (CAG→CCG)  rpoA ← RNA polymerase, alpha subunit
RA 3,498,239 A→C 11.9% intergenic (+97/+55) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,507,367 G→T 6.1% L128M (CTG→ATG)  gph ← phosphoglycolate phosphatase
RA 3,532,208 Δ1 bp 6.1% coding (1465/2322 nt) yhgF → putative transcriptional accessory protein
RA 3,532,209 Δ1 bp 6.1% coding (1466/2322 nt) yhgF → putative transcriptional accessory protein
RA 3,532,210 Δ1 bp 5.7% coding (1467/2322 nt) yhgF → putative transcriptional accessory protein
RA 3,565,480 G→T 14.4% T461T (ACC→ACA glgB ← 1,4‑alpha‑glucan branching enzyme
RA 3,586,364:1 +A 5.3% coding (434/714 nt) livF ← leucine/isoleucine/valine transporter subunit
RA 3,586,364:2 +G 5.3% coding (434/714 nt) livF ← leucine/isoleucine/valine transporter subunit
RA 3,586,364:3 +T 5.3% coding (434/714 nt) livF ← leucine/isoleucine/valine transporter subunit
RA 3,634,512 G→T 5.3% M97I (ATG→ATT dtpB → dipeptide and tripeptide permease B
RA 3,650,457 G→T 8.9% G35W (GGG→TGG)  hdeD → acid‑resistance membrane protein
RA 3,717,109 C→A 7.2% A217S (GCC→TCC)  glyS ← glycine tRNA synthetase, beta subunit
RA 3,742,844 C→A 5.7% R85S (CGT→AGT)  sgbU → putative L‑xylulose 5‑phosphate 3‑epimerase
RA 3,819,715 C→A 7.7% F382L (TTC→TTA recG → ATP‑dependent DNA helicase
RA 3,899,270 C→A 9.2% A220S (GCT→TCT)  bglG ← transcriptional antiterminator of the bgl operon
RA 3,899,591 C→T 5.4% V113M (GTG→ATG)  bglG ← transcriptional antiterminator of the bgl operon
RA 3,936,730 C→A 6.2% intergenic (+21/‑65) rrsC → / → gltU 16S ribosomal RNA of rrnC operon/tRNA‑Glu
RA 4,040,278 T→C 10.0% intergenic (‑316/‑48) yihG ← / → polA inner membrane protein, inner membrane acyltransferase/fused DNA polymerase I 5'‑>3' polymerase/3'‑>5' exonuclease/5'‑>3' exonuclease
RA 4,049,693 T→C 6.6% T3A (ACA→GCA)  glnL ← sensory histidine kinase in two‑component regulatory system with GlnG
RA 4,071,150 T→C 6.6% R114R (CGT→CGC yiiD → putative acetyltransferase
RA 4,091,025 G→T 5.3% Q96H (CAG→CAT sodA → superoxide dismutase, Mn
RA 4,100,153 A→G 8.4% N99S (AAC→AGC)  cdh → CDP‑diacylglycerol phosphotidylhydrolase
RA 4,125,079 G→A 5.7% P439P (CCG→CCA katG → catalase‑peroxidase HPI, heme b‑containing
RA 4,131,877 G→A 5.1% C91C (TGC→TGT frwA ← putative PTS enzyme, Hpr component/enzyme I component/enzyme IIA component
RA 4,165,449 C→T 19.4% noncoding (50/85 nt) tyrU → tRNA‑Tyr
RA 4,165,450 A→C 19.4% noncoding (51/85 nt) tyrU → tRNA‑Tyr
RA 4,166,006 C→T 15.8% R45R (CGC→CGT tufB → translation elongation factor EF‑Tu 2
RA 4,167,038 A→T 7.2% V389V (GTA→GTT tufB → translation elongation factor EF‑Tu 2
RA 4,167,041 A→T 7.6% A390A (GCA→GCT tufB → translation elongation factor EF‑Tu 2
RA 4,167,051 A→G 7.5% S394G (AGC→GGC)  tufB → translation elongation factor EF‑Tu 2
RA 4,177,142 A→T 5.3% D622V (GAC→GTC)  rpoC → RNA polymerase, beta prime subunit
RA 4,178,209 C→T 9.9% R978C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,199,631 T→A 10.6% intergenic (+15/‑71) rrsE → / → gltV 16S ribosomal RNA of rrnE operon/tRNA‑Glu
RA 4,215,580 C→T 5.8% P609S (CCC→TCC)  metH → homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
RA 4,289,072 G→A 5.3% Q38* (CAG→TAG)  fdhF ← formate dehydrogenase‑H, selenopolypeptide subunit
RA 4,329,684 C→A 5.8% G219C (GGT→TGT)  adiA ← arginine decarboxylase
RA 4,330,517 G→T 6.7% intergenic (‑179/+20) adiA ← / ← melR arginine decarboxylase/melibiose operon transcriptional regulator; autoregulator
RA 4,343,052 C→T 8.9% V495I (GTT→ATT)  lysU ← lysine tRNA synthetase, inducible
RA 4,382,345 G→T 7.6% noncoding (57/76 nt) glyX → tRNA‑Gly
RA 4,400,970 G→T 6.9% D201Y (GAC→TAC)  yjfJ → PspA/IM30 family protein
RA 4,432,075 G→T 11.1% intergenic (‑82/‑124) msrA ← / → tamA methionine sulfoxide reductase A/translocation and assembly module for autotransporter export, outer membrane subunit
RA 4,462,926 G→T 10.0% S34Y (TCC→TAC)  yjgH ← UPF0131 family protein
RA 4,498,906 T→C 9.1% pseudogene (81/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,498,909 G→T 9.5% pseudogene (84/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,533,877 G→T 11.1% E111D (GAG→GAT fimI → fimbrial protein involved in type 1 pilus biosynthesis
RA 4,565,972 G→A 6.1% pseudogene (2237/2937 nt) yjiV → pseudogene; conserved hypothetical protein
RA 4,626,148 C→A 8.4% R109S (CGC→AGC)  creB → response regulator in two‑component regulatory system with CreC

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 578865 579065 201 7 [6] [6] 7 tfaX/appY pseudogene, DLP12 prophage;Phage or Prophage Related/global transcriptional activator; DLP12 prophage
* * ÷ CP009273 2050210 2050282 73 8 [6] [6] 8 amn/yeeN AMP nucleosidase/UPF0082 family protein
* * ÷ CP009273 2462825 2463233 409 8 [6] [6] 7 gtrS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
* * ÷ CP009273 2988484 2988716 233 7 [6] [6] 8 [ygeN] [ygeN]
* * ÷ CP009273 3792468 3792906 439 7 [6] [6] 7 [waaU]–[waaZ] [waaU],[waaZ]
* * ÷ CP009273 3797617 3798024 408 8 [3] [5] 7 waaS lipopolysaccharide core biosynthesis protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =74 (1.130)90 (1.370) 31/250 NT 54.9% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 1203246 =55 (0.840)11 (0.190) 8/218 NT 25.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 15 (0.260)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326155 (0.840)32 (0.560) 13/218 NT 50.4% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 15 (0.260)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 3196596NA (NA)4 (0.060) 3/246 NT 8.3% noncoding (94/125 nt) REP225 (repetitive extragenic palindromic) element; contains 3 REP sequences REP225 (repetitive extragenic palindromic) element; contains 3 REP sequences
?CP009273 4206831 = 44 (0.680)coding (1426/1602 nt) aceB malate synthase A