breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7340GT35.6% 44.7 / 54.3 104intergenic (‑51/+257)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
*CP0092731,160,0270AC30.3% 183.8 / 19.8 99K159N (AAA→AACnagZbeta N‑acetyl‑glucosaminidase
*CP009273838,6520TG29.0% 190.9 / 29.5 124F289V (TTT→GTT) rlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
*CP009273357,0310CA25.6% 153.1 / 11.5 86V181F (GTT→TTT) lacAthiogalactoside acetyltransferase
*CP009273838,6930TG25.0% 286.2 / 13.8 125R302R (CGT→CGGrlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
*CP0092732,170,7460CA25.0% 162.7 / 16.4 106intergenic (‑63/+245)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
*CP009273357,0340GC24.4% 196.0 / 14.7 86R180G (CGG→GGG) lacAthiogalactoside acetyltransferase
*CP0092731,828,7270GC23.5% 246.5 / 13.6 101E119Q (GAA→CAA) ydjYputative ferrodoxin‑like lipoprotein
*CP0092732,170,7240GT22.9% 110.1 / 15.2 100intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
*CP0092731,160,0240GC22.7% 207.5 / 17.1 101M158I (ATG→ATCnagZbeta N‑acetyl‑glucosaminidase
*CP0092731,160,0020AT22.3% 223.1 / 13.6 97D151V (GAT→GTT) nagZbeta N‑acetyl‑glucosaminidase
*CP009273104,0410GT20.6% 133.2 / 10.3 63intergenic (+80/‑151)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
*CP009273104,0420AT20.0% 172.5 / 12.7 60intergenic (+81/‑150)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation

Marginal new junction evidence (lowest skew 10 of 12 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 453250784 (0.680)5 (0.040) 5/250 7.6 6.3% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?CP009273 = 4532803 70 (0.600)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? CP009273 = 1161581114 (0.920)4 (0.040) 4/238 7.9 3.5% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?CP009273 1162077 = 121 (1.100)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? CP009273 3086131 =68 (0.550)3 (0.020) 3/260 9.0 3.5% coding (895/951 nt) gshB glutathione synthetase
?CP009273 3215942 = 99 (0.820)intergenic (+134/‑50) ebgR/ebgA transcriptional repressor/evolved beta‑D‑galactosidase, alpha subunit
* ? CP009273 2605077 =121 (0.970)3 (0.020) 3/260 9.0 2.6% coding (262/414 nt) hyfJ putative processing element hydrogenase 4
?CP009273 2605264 = 104 (0.860)coding (6/2013 nt) hyfR hydrogenase‑4 transcriptional activator
* ? CP009273 = 360286NA (NA)3 (0.030)
+AATTC
3/258 9.0 2.1% intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 3416618 = 146 (1.180)intergenic (+65/+164) yhdZ/rrfF putative amino‑acid transporter subunit/5S ribosomal RNA of rrnD operon
* ? CP009273 = 122216692 (0.740)3 (0.020) 3/262 9.1 3.3% coding (111/369 nt) ycgJ uncharacterized protein
?CP009273 1222170 = 88 (0.730)coding (115/369 nt) ycgJ uncharacterized protein
* ? CP009273 = 665338104 (0.840)3 (0.020) 3/264 9.1 2.2% coding (26/612 nt) cobC putative alpha‑ribazole‑5'‑P phosphatase
?CP009273 = 665507 160 (1.310)coding (522/642 nt) nadD nicotinic acid mononucleotide adenylyltransferase, NAD(P)‑dependent
* ? CP009273 592877 =126 (1.010)3 (0.020) 3/264 9.1 2.2% coding (291/333 nt) cusF periplasmic copper‑ and silver‑binding protein
?CP009273 = 593125 139 (1.140)coding (191/1224 nt) cusB copper/silver efflux system, membrane fusion protein
* ? CP009273 = 89407152 (1.220)3 (0.020) 3/262 9.1 2.1% coding (1508/1767 nt) ftsI transpeptidase involved in septal peptidoglycan synthesis (penicillin‑binding protein 3)
?CP009273 = 368198 137 (1.130)coding (628/810 nt) mhpD 2‑keto‑4‑pentenoate hydratase
* ? CP009273 = 4323117147 (1.180)3 (0.020) 3/268 9.2 2.2% coding (651/669 nt) basR response regulator in two‑component regulatory system with BasS
?CP009273 4323122 = 126 (1.010)coding (646/669 nt) basR response regulator in two‑component regulatory system with BasS