breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 19,860 A→T 15.6% L152Q (CTG→CAG)  insB1 ← IS1 transposase B
RA 46,650 G→T 9.1% A282S (GCA→TCA)  yaaU → putative transporter
RA 60,953 C→T 7.3% R771H (CGT→CAT)  rapA ← RNA polymerase remodeling/recycling factor ATPase; RNA polymerase‑associated, ATP‑dependent RNA translocase
RA 114,781:1 +A 8.6% coding (352/894 nt) nadC ← quinolinate phosphoribosyltransferase
RA 114,781:2 +A 8.6% coding (352/894 nt) nadC ← quinolinate phosphoribosyltransferase
RA 114,781:3 +T 8.6% coding (352/894 nt) nadC ← quinolinate phosphoribosyltransferase
RA 114,782 G→A 8.6% G117G (GGC→GGT nadC ← quinolinate phosphoribosyltransferase
RA 114,784:1 +A 8.6% coding (349/894 nt) nadC ← quinolinate phosphoribosyltransferase
RA 114,784:2 +A 8.6% coding (349/894 nt) nadC ← quinolinate phosphoribosyltransferase
RA 114,784:3 +A 8.6% coding (349/894 nt) nadC ← quinolinate phosphoribosyltransferase
RA 114,785 C→A 8.6% T116T (ACG→ACT) ‡ nadC ← quinolinate phosphoribosyltransferase
RA 114,786 G→C 8.4% T116R (ACG→AGG) ‡ nadC ← quinolinate phosphoribosyltransferase
RA 114,791 C→T 8.6% L114L (CTG→CTA) ‡ nadC ← quinolinate phosphoribosyltransferase
RA 114,792 A→T 8.7% L114Q (CTG→CAG) ‡ nadC ← quinolinate phosphoribosyltransferase
RA 153,184 C→A 7.4% E63* (GAA→TAA)  yadN ← putative fimbrial‑like adhesin protein
RA 221,373:1 +G 9.0% noncoding (1116/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,380 T→A 9.1% noncoding (1123/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,390 Δ1 bp 9.3% noncoding (1133/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,391 G→A 9.3% noncoding (1134/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,393 C→T 9.6% noncoding (1136/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,395 G→A 9.4% noncoding (1138/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,397 C→T 9.5% noncoding (1140/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,398 C→T 9.5% noncoding (1141/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,402 A→C 9.9% noncoding (1145/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,407 A→T 10.4% noncoding (1150/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,412 A→T 10.5% noncoding (1155/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,420 A→G 10.9% noncoding (1163/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,425 T→C 16.9% noncoding (1168/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,430 T→C 17.2% noncoding (1173/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,455 G→A 23.7% noncoding (1198/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,464 G→C 21.7% noncoding (1207/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,471 C→T 20.0% noncoding (1214/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,476 A→T 22.9% noncoding (1219/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 245,377 C→A 8.8% pseudogene (1181/1713 nt) lfhA ← pseudogene, flagellar system protein, promoterless fragment; flagellar biosynthesis
RA 274,938 T→A 5.2% Q152L (CAG→CTG)  insB1 ← IS1 transposase B
RA 295,695 T→G 5.7% E317D (GAA→GAC paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit
RA 335,782 A→G 5.1% intergenic (+328/‑94) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein
RA 370,602 G→A 6.9% intergenic (+265/‑313) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,617 Δ1 bp 5.6% intergenic (+280/‑298) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,618 Δ1 bp 5.6% intergenic (+281/‑297) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,619 T→G 5.6% intergenic (+282/‑296) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,621 Δ1 bp 6.2% intergenic (+284/‑294) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,626 Δ1 bp 6.2% intergenic (+289/‑289) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,627 Δ1 bp 7.0% intergenic (+290/‑288) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,628 Δ1 bp 6.7% intergenic (+291/‑287) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,632 A→G 7.6% intergenic (+295/‑283) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,658 T→C 7.9% intergenic (+321/‑257) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,659 T→A 7.7% intergenic (+322/‑256) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 385,480 T→A 7.5% intergenic (‑297/‑227) hemB ← / → yaiT 5‑aminolevulinate dehydratase (porphobilinogen synthase)/pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
RA 393,386 G→A 10.2% G20E (GGG→GAG)  yaiW → putative lipoprotein
RA 436,987 C→A 19.9% intergenic (‑188/‑18) xseB ← / → thiI exonuclease VII small subunit/tRNA s(4)U8 sulfurtransferase
RA 459,279 G→T 7.7% intergenic (+37/‑114) ppiD → / → ybaV periplasmic folding chaperone, has an inactive PPIase domain/putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein
RA 573,883 G→C 11.0% T107T (ACG→ACC
R34P (CGG→CCG) 
rzpD →
rzoD →
DLP12 prophage; putative murein endopeptidase
DLP12 prophage; putative lipoprotein
RA 727,369 A→G 6.1% A777A (GCA→GCG rhsC → Rhs family putative polymorphic toxin
RA 727,375 A→G 6.2% G779G (GGA→GGG rhsC → Rhs family putative polymorphic toxin
RA 727,989 C→A 8.4% A984E (GCA→GAA)  rhsC → Rhs family putative polymorphic toxin
RA 730,138 A→G 7.5% pseudogene (550/1521 nt) rhsO → pseudogene, Rhs family protein
RA 731,587 G→T 7.6% R161L (CGA→CTA)  ybfC → putative periplasmic protein
RA 733,211 T→A 6.0% pseudogene (931/1137 nt) ybfL → pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
RA 733,372 T→C 7.8% pseudogene (1092/1137 nt) ybfL → pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
RA 733,384 C→G 15.7% pseudogene (1104/1137 nt) ybfL → pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
RA 734,165 C→T 5.9% F206F (TTC→TTT ybfD → H repeat‑associated putative transposase
RA 811,110 C→T 7.5% intergenic (+107/+85) uvrB → / ← ybhK excinulease of nucleotide excision repair, DNA damage recognition component/putative NAD(P)‑binding transferase
RA 833,739 G→T 6.3% R58R (CGC→CGA ybiI ← DksA‑type zinc finger protein
RA 839,517 Δ1 bp 6.5% coding (1420/2226 nt) ybiO ← mechanosensitive channel protein, intermediate conductance
RA 839,518 Δ1 bp 6.5% coding (1419/2226 nt) ybiO ← mechanosensitive channel protein, intermediate conductance
RA 839,519 Δ1 bp 6.5% coding (1418/2226 nt) ybiO ← mechanosensitive channel protein, intermediate conductance
RA 839,520 Δ1 bp 6.0% coding (1417/2226 nt) ybiO ← mechanosensitive channel protein, intermediate conductance
RA 849,308 G→A 6.8% R69H (CGC→CAC)  ybiR → putative transporter
RA 857,390 C→A 5.3% A225S (GCC→TCC)  ybiW ← putative pyruvate formate lyase
RA 882,776 C→A 7.2% R7L (CGT→CTT)  ybjJ ← putative transporter
RA 900,616 G→T 7.3% G83V (GGT→GTT)  amiD → 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase, Zn‑dependent; OM lipoprotein
RA 904,382 G→A 5.8% D123D (GAC→GAT ltaE ← L‑allo‑threonine aldolase, PLP‑dependent
RA 940,636:1 +G 5.8% coding (378/627 nt) ycaC ← putative isochorismatase family hydrolase
RA 940,636:2 +G 5.8% coding (378/627 nt) ycaC ← putative isochorismatase family hydrolase
RA 940,636:3 +C 5.8% coding (378/627 nt) ycaC ← putative isochorismatase family hydrolase
RA 940,638:1 +T 6.2% coding (376/627 nt) ycaC ← putative isochorismatase family hydrolase
RA 940,638:2 +A 6.2% coding (376/627 nt) ycaC ← putative isochorismatase family hydrolase
RA 940,638:3 +G 6.2% coding (376/627 nt) ycaC ← putative isochorismatase family hydrolase
RA 965,548 G→T 6.2% R235L (CGT→CTT)  ycaQ → DUF1006 family protein with C‑terminal wHTH domain
RA 980,627 C→A 6.1% C180F (TGC→TTC)  aspC ← aspartate aminotransferase, PLP‑dependent
RA 1,074,375 C→A 6.1% intergenic (‑37/‑386) putA ← / → putP fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase/proline:sodium symporter
RA 1,134,840 T→C 5.4% L336P (CTG→CCG)  flgK → flagellar hook‑filament junction protein 1
RA 1,161,605 C→A 12.5% T22K (ACA→AAA)  ndh → respiratory NADH dehydrogenase 2/cupric reductase
RA 1,169,398 G→A 5.3% T8M (ACG→ATG)  mfd ← transcription‑repair coupling factor
RA 1,178,411 G→T 6.9% V222V (GTC→GTA potC ← polyamine transporter subunit
RA 1,282,249 A→G 9.2% intergenic (+267/+273) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,497 A→G 6.9% intergenic (+515/+25) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,323,001 G→T 7.0% V123V (GTC→GTA yciK ← putative EmrKY‑TolC system oxoacyl‑(acyl carrier protein) reductase
RA 1,354,830 G→T 6.7% A112A (GCC→GCA puuA ← glutamate‑‑putrescine ligase
RA 1,422,040 A→G 12.1% pseudogene (944/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,060 G→C 12.8% pseudogene (924/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,150 C→T 7.6% pseudogene (834/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,252 A→G 5.9% pseudogene (732/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,426,270 A→G 5.4% K989E (AAA→GAA)  stfR → Rac prophage; putative tail fiber protein
RA 1,437,441 C→T 7.7% A45V (GCA→GTA)  ydbH → putative membrane‑anchored protein, function unknown
RA 1,445,629 G→T 7.3% intergenic (‑23/+225) tynA ← / ← paaZ tyramine oxidase, copper‑requiring/fused oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
RA 1,447,443 G→A 10.2% H153Y (CAT→TAT)  paaZ ← fused oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
RA 1,460,819 G→A 10.5% pseudogene (1171/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,484,659 C→A 11.4% pseudogene (312/750 nt) gapC ← pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,491,267 C→T 8.0% S52L (TCA→TTA)  opgD → osmoregulated periplasmic glucan (OPG) biosynthesis periplasmic protein
RA 1,507,299 C→T 5.9% R76W (CGG→TGG)  ydcT → putative spermidine/putrescine transporter subunit
RA 1,515,524 G→A 5.5% A600V (GCG→GTG)  yncD ← putative iron outer membrane transporter
RA 1,527,173 C→T 7.9% Y367Y (TAC→TAT ydcC → H repeat‑associated putative transposase
RA 1,527,189 A→G 7.7% T373A (ACG→GCG)  ydcC → H repeat‑associated putative transposase
RA 1,564,253 A→G 6.7% L165P (CTG→CCG)  gadC ← glutamate:gamma‑aminobutyric acid antiporter
RA 1,574,849 C→T 15.4% G67S (GGC→AGC)  ydeM ← putative YdeN‑specific sulfatase‑maturating enzyme
RA 1,582,609 C→A 7.1% G163V (GGA→GTA)  ydeS ← putative fimbrial‑like adhesin protein
RA 1,630,830 A→G 10.2% intergenic (‑206/‑183) nohQ ← / → ynfO pseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin/uncharacterized protein, Qin prophage
RA 1,754,887 T→A 10.5% M388L (ATG→TTG)  sufD ← component of SufBCD Fe‑S cluster assembly scaffold
RA 1,770,867 T→C 5.0% S342P (TCA→CCA)  ydiF → putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit
RA 1,869,685 G→A 10.2% A50V (GCA→GTA)  yeaM ← putative DNA‑binding transcriptional regulator
RA 1,875,761 G→T 6.6% S102R (AGC→AGA dmlR ← transcriptional activator of dmlA
RA 1,906,184 A→G 5.1% F217S (TTC→TCC)  htpX ← putative endopeptidase
RA 1,913,736 Δ1 bp 5.8% coding (1970/2634 nt) yebT → MCE domain protein
RA 1,913,737 Δ1 bp 5.8% coding (1971/2634 nt) yebT → MCE domain protein
RA 1,913,738 Δ1 bp 5.8% coding (1972/2634 nt) yebT → MCE domain protein
RA 1,913,739 Δ1 bp 5.8% coding (1973/2634 nt) yebT → MCE domain protein
RA 1,913,740 Δ1 bp 5.8% coding (1974/2634 nt) yebT → MCE domain protein
RA 1,941,588 A→G 7.5% Y16H (TAC→CAC)  ruvC ← component of RuvABC resolvasome, endonuclease
RA 1,941,589 G→A 8.0% G15G (GGC→GGT ruvC ← component of RuvABC resolvasome, endonuclease
RA 1,944,310 A→G 5.5% F157S (TTC→TCC)  aspS ← aspartyl‑tRNA synthetase
RA 1,952,263 G→T 10.0% H43N (CAC→AAC)  torY ← TMAO reductase III (TorYZ), cytochrome c‑type subunit
RA 2,001,467 C→A 15.7% A35A (GCG→GCT yedD ← lipoprotein
RA 2,004,485 G→T 8.8% P135P (CCG→CCT yedL → putative acyl transferase
RA 2,064,944 T→C 6.7% noncoding (149/204 nt) isrC → novel sRNA, CP4‑44; putative prophage remnant
RA 2,064,946:1 +A 6.3% noncoding (151/204 nt) isrC → novel sRNA, CP4‑44; putative prophage remnant
RA 2,064,946:2 +C 6.3% noncoding (151/204 nt) isrC → novel sRNA, CP4‑44; putative prophage remnant
RA 2,064,946:3 +A 6.3% noncoding (151/204 nt) isrC → novel sRNA, CP4‑44; putative prophage remnant
RA 2,064,949 C→T 6.2% noncoding (154/204 nt) isrC → novel sRNA, CP4‑44; putative prophage remnant
RA 2,093,894 C→A 7.4% A286S (GCA→TCA)  gnd ← 6‑phosphogluconate dehydrogenase, decarboxylating
RA 2,126,266 G→T 11.8% L205M (CTG→ATG)  wcaA ← putative glycosyl transferase
RA 2,167,353 C→T 10.2% L135L (TTG→TTA gatC ← galactitol PTS permease ‑ GatC subunit
RA 2,201,723:1 +G 5.4% coding (278/1137 nt) yehP → VMA domain putative YehL ATPase stimulator
RA 2,201,723:2 +C 5.4% coding (278/1137 nt) yehP → VMA domain putative YehL ATPase stimulator
RA 2,201,723:3 +T 5.4% coding (278/1137 nt) yehP → VMA domain putative YehL ATPase stimulator
RA 2,201,723:4 +G 5.4% coding (278/1137 nt) yehP → VMA domain putative YehL ATPase stimulator
RA 2,201,723:5 +C 5.4% coding (278/1137 nt) yehP → VMA domain putative YehL ATPase stimulator
RA 2,213,979 T→C 7.3% Q497R (CAG→CGG)  bglX ← beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,276,396 T→C 6.0% intergenic (+116/+23) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
RA 2,276,402 Δ1 bp 5.9% intergenic (+122/+17) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
RA 2,276,403 Δ1 bp 5.9% intergenic (+123/+16) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
RA 2,276,404 Δ1 bp 6.1% intergenic (+124/+15) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
RA 2,276,406 Δ1 bp 6.3% intergenic (+126/+13) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
RA 2,276,407 Δ1 bp 6.5% intergenic (+127/+12) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
RA 2,276,408 Δ1 bp 6.6% intergenic (+128/+11) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
RA 2,276,409 Δ1 bp 6.6% intergenic (+129/+10) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
RA 2,276,410 Δ1 bp 6.6% intergenic (+130/+9) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
RA 2,391,037 G→T 5.7% P870T (CCG→ACG)  nuoG ← NADH:ubiquinone oxidoreductase, chain G
RA 2,393,971 G→C 5.2% T355R (ACG→AGG)  nuoF ← NADH:ubiquinone oxidoreductase, chain F
RA 2,393,974 Δ1 bp 5.2% coding (1061/1338 nt) nuoF ← NADH:ubiquinone oxidoreductase, chain F
RA 2,393,975 Δ1 bp 5.2% coding (1060/1338 nt) nuoF ← NADH:ubiquinone oxidoreductase, chain F
RA 2,393,976 Δ1 bp 5.2% coding (1059/1338 nt) nuoF ← NADH:ubiquinone oxidoreductase, chain F
RA 2,399,955 C→A 100% E56* (GAA→TAA)  lrhA ← transcriptional repressor of flagellar, motility and chemotaxis genes
RA 2,439,357 C→T 9.8% Q169Q (CAG→CAA mepA ← murein DD‑endopeptidase
RA 2,457,796 T→C 6.2% K231E (AAA→GAA)  mlaA ← ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component
RA 2,483,212 G→T 9.6% P152T (CCT→ACT)  yfdV ← putative transporter
RA 2,593,307 G→T 9.7% G15C (GGT→TGT)  gcvR → transcriptional repressor, regulatory protein accessory to GcvA
RA 2,615,169 G→T 9.8% G205V (GGT→GTT)  purM → phosphoribosylaminoimidazole synthetase
RA 2,661,478 C→T 7.1% G260R (GGA→AGA)  hcaR ← hca operon transcriptional regulator
RA 2,663,580 G→T 5.3% G397V (GGG→GTG)  hcaE → 3‑phenylpropionate dioxygenase, large (alpha) subunit
RA 2,699,643 C→A 11.7% A281S (GCA→TCA)  lepA ← back‑translocating elongation factor EF4, GTPase
RA 2,707,703 C→T 7.7% intergenic (+114/+95) srmB → / ← yfiE ATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator
RA 2,719,426 T→C 16.6% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,726,461 C→A 5.7% E358* (GAA→TAA)  clpB ← protein disaggregation chaperone
RA 2,771,529 T→C 9.6% V1519V (GTA→GTG ypjA ← adhesin‑like autotransporter
RA 2,816,372 C→A 17.3% A253S (GCT→TCT)  recA ← DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein forming filaments; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor
RA 2,821,435 G→T 5.5% V114L (GTG→TTG)  srlD → sorbitol‑6‑phosphate dehydrogenase
RA 2,823,738 G→T 7.8% G189G (GGG→GGT srlQ → D‑arabinose 5‑phosphate isomerase
RA 2,824,259 G→T 10.0% Q464K (CAG→AAG)  norR ← anaerobic nitric oxide reductase DNA‑binding transcriptional activator
RA 2,825,440 A→G 6.1% L70P (CTG→CCG)  norR ← anaerobic nitric oxide reductase DNA‑binding transcriptional activator
RA 2,873,415 C→T 5.3% R111H (CGC→CAC)  casE ← CRISPR RNA precursor cleavage enzyme; CRISP RNA (crRNA) containing Cascade antiviral complex protein
RA 3,005,901 T→A 7.6% intergenic (+149/+72) yqeA → / ← yqeB putative amino acid kinase/XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold
RA 3,037,084:1 +G 5.3% coding (64/1440 nt) bglA → 6‑phospho‑beta‑glucosidase A
RA 3,037,084:2 +G 5.3% coding (64/1440 nt) bglA → 6‑phospho‑beta‑glucosidase A
RA 3,037,084:3 +C 5.3% coding (64/1440 nt) bglA → 6‑phospho‑beta‑glucosidase A
RA 3,037,084:4 +G 5.3% coding (64/1440 nt) bglA → 6‑phospho‑beta‑glucosidase A
RA 3,037,084:5 +C 5.3% coding (64/1440 nt) bglA → 6‑phospho‑beta‑glucosidase A
RA 3,037,084:6 +G 5.3% coding (64/1440 nt) bglA → 6‑phospho‑beta‑glucosidase A
RA 3,037,086 G→C 5.3% Q22H (CAG→CAC bglA → 6‑phospho‑beta‑glucosidase A
RA 3,037,088:1 +C 5.3% coding (68/1440 nt) bglA → 6‑phospho‑beta‑glucosidase A
RA 3,037,088:2 +A 5.3% coding (68/1440 nt) bglA → 6‑phospho‑beta‑glucosidase A
RA 3,037,088:3 +G 5.3% coding (68/1440 nt) bglA → 6‑phospho‑beta‑glucosidase A
RA 3,037,088:4 +G 5.3% coding (68/1440 nt) bglA → 6‑phospho‑beta‑glucosidase A
RA 3,037,234 C→A 6.1% H72N (CAC→AAC)  bglA → 6‑phospho‑beta‑glucosidase A
RA 3,064,718 T→G 13.6% intergenic (‑115/+100) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,106,358 C→A 8.8% intergenic (‑58/+68) yghF ← / ← yghG pseudogene, secretion pathway protein, C‑type protein homology;putative transport; Not classified; putative general secretion pathway for protein export (GSP)/secretin (GspDbeta) OM localization lipoprotein pilotin
RA 3,135,976 C→T 5.8% A125T (GCT→ACT)  hybC ← hydrogenase 2, large subunit
RA 3,142,666 G→A 5.8% L55L (CTC→CTT yqhA ← UPF0114 family putative inner membrane protein
RA 3,158,532 C→T 6.4% L267L (CTG→CTA parC ← DNA topoisomerase IV, subunit A
RA 3,169,995 C→T 8.9% A66A (GCG→GCA cpdA ← 3',5' cAMP phosphodiesterase
RA 3,237,555 C→A 6.5% P182P (CCG→CCT uxaC ← uronate isomerase
RA 3,263,315 T→C 12.6% intergenic (+354/+260) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,284,921 G→T 7.8% A74A (GCG→GCT yraK → putative fimbrial‑like adhesin protein
RA 3,308,353 C→T 5.1% A341T (GCA→ACA)  infB ← translation initiation factor IF‑2
RA 3,327,782 G→A 7.4% L238L (CTG→CTA ispB → octaprenyl diphosphate synthase
RA 3,403,099 C→A 11.8% A224D (GCC→GAC)  prmA → methyltransferase for 50S ribosomal subunit protein L11
RA 3,416,890 T→G 10.8% noncoding (12/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,416,899 A→G 13.5% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,446,133 G→A 7.3% T51T (ACC→ACT rplC ← 50S ribosomal subunit protein L3
RA 3,464,590 G→A 7.6% T33T (ACC→ACT tufA ← translation elongation factor EF‑Tu 1
RA 3,469,331 C→A 10.0% V157F (GTC→TTC)  yheO ← putative PAS domain‑containing DNA‑binding transcriptional regulator
RA 3,589,089 C→A 5.2% Q225H (CAG→CAT livH ← leucine/isoleucine/valine transporter subunit
RA 3,594,840 C→G 6.4% E203Q (GAA→CAA)  ftsX ← inner membrane putative ABC superfamily transporter permease
RA 3,616,199 A→G 5.1% R1216R (CGA→CGG rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,618,160 A→G 13.4% G141G (GGA→GGG yhhI → putative transposase
RA 3,686,001 C→T 5.3% D866N (GAT→AAT)  bcsA ← cellulose synthase, catalytic subunit
RA 3,702,353 A→G 5.3% W495R (TGG→CGG)  eptB ← KDO phosphoethanolamine transferase, Ca(2+)‑inducible
RA 3,782,486 C→A 5.3% W319L (TGG→TTG)  waaH ← LPS(HepIII)‑glucuronic acid glycosyltransferase
RA 3,804,818 C→A 5.2% A73S (GCC→TCC)  rpmB ← 50S ribosomal subunit protein L28
RA 3,813,247 C→A 6.6% L428L (CTG→CTT ligB ← DNA ligase, NAD(+)‑dependent
RA 3,912,503 G→A 5.2% P239S (CCG→TCG)  atpA ← F1 sector of membrane‑bound ATP synthase, alpha subunit
RA 3,917,110 G→T 5.3% S628R (AGC→AGA mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
RA 3,936,724 T→A 9.0% intergenic (+15/‑71) rrsC → / → gltU 16S ribosomal RNA of rrnC operon/tRNA‑Glu
RA 3,936,730 C→T 6.2% intergenic (+21/‑65) rrsC → / → gltU 16S ribosomal RNA of rrnC operon/tRNA‑Glu
RA 3,971,455 T→C 5.9% S191P (TCC→CCC)  wecF → TDP‑Fuc4NAc:lipidIIFuc4NAc transferase
RA 4,030,856 A→T 14.9% intergenic (+161/‑23) alaT → / → rrlA tRNA‑Ala/23S ribosomal RNA of rrnA operon
RA 4,143,523 A→C 8.1% intergenic (‑497/+101) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase
RA 4,165,449 C→T 34.6% noncoding (50/85 nt) tyrU → tRNA‑Tyr
RA 4,165,450 A→C 34.5% noncoding (51/85 nt) tyrU → tRNA‑Tyr
RA 4,177,142 A→G 24.0% D622G (GAC→GGC)  rpoC → RNA polymerase, beta prime subunit
RA 4,178,500 C→T 27.0% R1075C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,199,631 T→A 7.5% intergenic (+15/‑71) rrsE → / → gltV 16S ribosomal RNA of rrnE operon/tRNA‑Glu
RA 4,202,979 A→C 17.1% noncoding (12/120 nt) rrfE → 5S ribosomal RNA of rrnE operon
RA 4,239,325:1 +A 13.0% intergenic (+86/‑157) lamB → / → malM maltose outer membrane porin (maltoporin)/maltose regulon periplasmic protein
RA 4,239,328 C→A 12.4% intergenic (+89/‑154) lamB → / → malM maltose outer membrane porin (maltoporin)/maltose regulon periplasmic protein
RA 4,239,329 G→C 16.8% intergenic (+90/‑153) lamB → / → malM maltose outer membrane porin (maltoporin)/maltose regulon periplasmic protein
RA 4,239,332 T→A 11.8% intergenic (+93/‑150) lamB → / → malM maltose outer membrane porin (maltoporin)/maltose regulon periplasmic protein
RA 4,239,333 G→C 12.0% intergenic (+94/‑149) lamB → / → malM maltose outer membrane porin (maltoporin)/maltose regulon periplasmic protein
RA 4,239,335 Δ1 bp 12.2% intergenic (+96/‑147) lamB → / → malM maltose outer membrane porin (maltoporin)/maltose regulon periplasmic protein
RA 4,239,337 A→C 12.0% intergenic (+98/‑145) lamB → / → malM maltose outer membrane porin (maltoporin)/maltose regulon periplasmic protein
RA 4,239,338 A→C 14.8% intergenic (+99/‑144) lamB → / → malM maltose outer membrane porin (maltoporin)/maltose regulon periplasmic protein
RA 4,239,339 T→C 11.1% intergenic (+100/‑143) lamB → / → malM maltose outer membrane porin (maltoporin)/maltose regulon periplasmic protein
RA 4,239,345 C→T 11.3% intergenic (+106/‑137) lamB → / → malM maltose outer membrane porin (maltoporin)/maltose regulon periplasmic protein
RA 4,239,359 T→C 9.8% intergenic (+120/‑123) lamB → / → malM maltose outer membrane porin (maltoporin)/maltose regulon periplasmic protein
RA 4,315,765 A→G 7.6% intergenic (‑207/+451) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,316,036:1 +G 6.0% intergenic (‑478/+180) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,343,151 C→T 8.7% V462M (GTG→ATG)  lysU ← lysine tRNA synthetase, inducible
RA 4,369,772 C→A 5.9% G188V (GGT→GTT)  frdB ← fumarate reductase (anaerobic), Fe‑S subunit
RA 4,370,978 C→T 6.1% L386L (CTG→CTA frdA ← fumarate reductase (anaerobic) catalytic and NAD/flavoprotein subunit
RA 4,399,395 G→A 7.0% G102S (GGC→AGC)  rlmB → 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
RA 4,405,345 G→T 10.8% M418I (ATG→ATT aidB → DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
RA 4,423,658 C→T 10.4% A62T (GCA→ACA)  qorB ← NAD(P)H:quinone oxidoreductase
RA 4,442,581 C→T 13.6% P65L (CCC→CTC)  ytfT → inner membrane putative ABC superfamily sugar transporter permease
RA 4,449,187 T→C 6.9% intergenic (+59/‑120) pmbA → / → cybC putative antibiotic peptide MccB17 maturation peptidase/pseudogene, truncated cytochrome b562; cytochrome b(562)
RA 4,498,909 G→T 5.1% pseudogene (84/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,826 G→A 5.1% pseudogene (289/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,830 A→G 5.3% pseudogene (293/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,577,448 G→T 7.4% G228V (GGC→GTC)  mrr → methylated adenine and cytosine restriction protein
RA 4,578,122 G→T 6.9% H187Q (CAC→CAA yjiA ← GTP‑binding protein, putative GTPase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 2208347 2209079 733 9 [7] [6] 8 [mlrA] [mlrA]
* * ÷ CP009273 2463643 2463814 172 11 [7] [6] 8 gtrS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
* * ÷ CP009273 3448799 3448865 67 9 [5] [7] 20 gspA/gspC general secretory pathway component, cryptic/general secretory pathway component, cryptic

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 335746 =134 (2.010)11 (0.170) 6/246 NT 7.6% noncoding (279/358 nt) REP23 (repetitive extragenic palindromic) element; contains 8 REP sequences REP23 (repetitive extragenic palindromic) element; contains 8 REP sequences
?CP009273 372778 = NA (NA)intergenic (+11/+213) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 360104 =51 (0.760)62 (0.930) 27/248 NT 54.9% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 = 37038554 (1.120)15 (0.340) 5/162 NT 23.6% intergenic (+48/‑530) mhpE/mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
?CP009273 = 372977 NA (NA)noncoding (187/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? CP009273 = 37041481 (1.210)6 (0.130) 3/174 NT 9.9% noncoding (29/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 = 372848 52 (1.110)noncoding (58/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? CP009273 = 37048685 (1.760)10 (0.230) 7/164 NT 11.4% noncoding (101/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 = 372977 NA (NA)noncoding (187/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? CP009273 1203246 =18 (0.270)34 (0.590) 19/216 NT 70.3% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 13 (0.220)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326119 (0.280)25 (0.430) 11/216 NT 62.9% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 13 (0.220)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related