breseq  version 0.35.4  revision f352f80f4bc9 mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 53 shown, sorted by frequency from high to low)
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,177,245	0	G	T	65.0%	‑0.5 / 19.8	45	intergenic (‑41/+267)	gatY/fbaB	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*	NC_000913	454,906	0	G	T	58.3%	4.0 / 22.1	30	intergenic (+117/‑227)	bolA/tig	DNA‑binding transcriptional dual regulator BolA/trigger factor
*	NC_000913	3,070,983	0	T	G	54.0%	67.3 / 158.0	113	I88L (ATC→CTC)	fbaA	fructose‑bisphosphate aldolase class II
*	NC_000913	2,177,234	0	C	A	51.7%	0.2 / 21.3	42	intergenic (‑30/+278)	gatY/fbaB	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*	NC_000913	729,372	0	A	C	50.0%	‑0.9 / 10.3	35	intergenic (+32/‑211)	ybfA/rhsC	DUF2517 domain‑containing protein YbfA/rhs element protein RhsC
*	NC_000913	1,879,846	0	T	G	50.0%	7.9 / 16.1	32	T35P (ACG→CCG)	yeaR	DUF1971 domain‑containing protein YeaR
*	NC_000913	18,110	0	T	G	45.8%	7.1 / 14.7	24	Y208D (TAT→GAT)	nhaA	Na(+):H(+) antiporter NhaA
*	NC_000913	2,074,681	1	.	G	44.4%	‑4.2 / 11.8	9	intergenic (+23/‑98)	flu/yeeR	CP4‑44 prophage; self recognizing antigen 43 (Ag43) autotransporter/CP4‑44 prophage; inner membrane protein YeeR
*	NC_000913	3,879,427	0	C	G	43.3%	16.4 / 12.0	33	K231N (AAG→AAC)	gyrB	DNA gyrase subunit B
*	NC_000913	1,683,038	0	G	T	42.9%	51.2 / 11.9	42	E53* (GAA→TAA)	rstB	sensory histidine kinase RstB
*	NC_000913	454,934	0	A	C	42.4%	23.7 / 12.3	33	intergenic (+145/‑199)	bolA/tig	DNA‑binding transcriptional dual regulator BolA/trigger factor
*	NC_000913	2,177,255	0	G	T	42.0%	9.4 / 31.3	50	intergenic (‑51/+257)	gatY/fbaB	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*	NC_000913	454,926	0	G	A	41.2%	6.7 / 14.6	30	intergenic (+137/‑207)	bolA/tig	DNA‑binding transcriptional dual regulator BolA/trigger factor
*	NC_000913	707,981	0	C	T	41.2%	8.3 / 12.6	25	intergenic (+224/‑353)	glnS/chiP	glutamine‑‑tRNA ligase/chitobiose outer membrane channel
*	NC_000913	707,989	0	C	T	41.2%	11.8 / 11.6	25	intergenic (+232/‑345)	glnS/chiP	glutamine‑‑tRNA ligase/chitobiose outer membrane channel
*	NC_000913	1,759,036	0	G	C	40.6%	43.2 / 17.1	32	V85V (GTC→GTG)	sufE	sulfur acceptor for SufS cysteine desulfurase
*	NC_000913	361,305	0	T	G	39.5%	37.1 / 22.9	38	N186H (AAC→CAC)	lacA	galactoside O‑acetyltransferase
*	NC_000913	1,759,035	0	A	C	38.7%	40.9 / 13.5	31	F86V (TTT→GTT)	sufE	sulfur acceptor for SufS cysteine desulfurase
*	NC_000913	4,439,033	0	T	G	38.6%	50.5 / 19.4	44	N77T (AAC→ACC)	ytfJ	protein YtfJ
*	NC_000913	411,595	0	G	C	37.0%	51.2 / 19.3	56	A296G (GCT→GGT)	araJ	putative transport protein AraJ

Marginal new junction evidence (sorted from low to high skew)
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4498181 =	NA (NA)	7 (0.120)	5/256	4.7	NA	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	= 4499512	NA (NA)					intergenic (+12/+81)	insD‑6/yjgX	KpLE2 phage‑like element; IS2 insertion element protein InsB/KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment
*	?	NC_000913	381258 =	NA (NA)	10 (0.170)	5/254	4.7	NA	pseudogene (3/417 nt)	yaiX	putative acyltransferase, N‑terminal fragment
	?	NC_000913	= 382589	NA (NA)					noncoding (1330/1331 nt)	IS2	repeat region