breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 51,870 C→G 10.2% V187V (GTG→GTC rsmA ← 16S rRNA m(6)2A1518,m(6)2A1519 dimethyltransferase
RA 88,161 A→G 10.2% H45R (CAC→CGC)  cra → DNA‑binding transcriptional dual regulator Cra
RA 238,497 G→T 21.6% I80I (ATC→ATA ykfM ← uncharacterized protein YkfM
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
RA 307,186 G→T 12.5% S716* (TCA→TAA)  ecpC ← putative fimbrial usher protein EcpC
RA 492,044 A→G 13.3% intergenic (+81/‑48) apt → / → dnaX adenine phosphoribosyltransferase/DNA polymerase III subunit tau
RA 626,998 C→T 11.1% A310V (GCA→GTA)  entE → 2,3‑dihydroxybenzoate‑AMP ligase
RA 744,378 Δ1 bp 12.9% coding (136/933 nt) pxpC → 5‑oxoprolinase component C
MC JC 932,613 Δ459 bp 100% coding (19‑477/495 nt) lrp → DNA‑binding transcriptional dual regulator Lrp
RA 1,109,125 Δ1 bp 10.5% intergenic (‑184/‑210) opgC ← / → opgG protein required for succinyl modification of osmoregulated periplasmic glucans/osmoregulated periplasmic glucans (OPGs) biosynthesis protein G
RA 1,137,190 C→T 9.5% P306L (CCA→CTA)  flgI → flagellar P‑ring protein
RA 1,252,563 G→C 13.6% G500R (GGC→CGC)  dhaR → DNA‑binding transcriptional dual regulator DhaR
RA 1,277,302 C→T 12.1% W106* (TGG→TAG)  narX ← sensory histidine kinase NarX
RA 1,398,448 C→T 10.8% A59T (GCT→ACT)  uspE ← universal stress protein with a role cellular motility
RA 1,492,979 Δ1 bp 10.3% coding (510/1641 nt) trg → methyl‑accepting chemotaxis protein Trg
RA 1,492,980 Δ1 bp 10.3% coding (511/1641 nt) trg → methyl‑accepting chemotaxis protein Trg
RA 1,492,981 Δ1 bp 10.3% coding (512/1641 nt) trg → methyl‑accepting chemotaxis protein Trg
RA 1,492,982 Δ1 bp 10.3% coding (513/1641 nt) trg → methyl‑accepting chemotaxis protein Trg
RA 1,701,239 T→A 10.0% S95T (TCT→ACT)  malY → negative regulator of MalT activity/cystathionine beta‑lyase
RA 1,836,740 T→C 13.1% V223A (GTC→GCC)  ynjB → putative ABC transporter periplasmic binding protein YnjB
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
RA 2,083,752 Δ1 bp 13.3% coding (997/1428 nt) sbcB → exodeoxyribonuclease I
RA 2,138,040 C→T 9.8% R47W (CGG→TGG)  yegH → inner membrane protein YegH
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,177,245 G→T 52.8% intergenic (‑41/+267) gatY ← / ← fbaB tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
RA 2,224,148 A→G 13.8% C241C (TGT→TGC pbpG ← peptidoglycan DD‑endopeptidase PbpG
RA 2,490,148 G→A 8.3% I13I (ATC→ATT yfdV ← putative transport protein YfdV
RA 2,829,156 G→A 12.5% R37H (CGC→CAC)  srlR → DNA‑binding transcriptional repressor SrlR
RA 3,021,851 C→T 5.1% T179I (ACT→ATT)  xdhD → fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit
RA 3,115,949 G→A 5.6% P1055L (CCG→CTG)  yghJ ← putative lipoprotein YghJ
RA 3,117,310 C→G 6.4% K601N (AAG→AAC yghJ ← putative lipoprotein YghJ
RA 3,166,424 T→C 5.2% Q432R (CAG→CGG)  ygiS ← putative deoxycholate binding periplasmic protein
RA 3,174,383 A→G 5.5% S338S (TCT→TCC parE ← DNA topoisomerase IV subunit B
RA 3,188,976 C→A 21.5% pseudogene (1545/2434 nt) yqiG → putative outer membrane usher protein YqiG
RA 3,418,500 T→A 31.0% N37K (AAT→AAA yhdV → lipoprotein YhdV
RA 3,447,122 A→T 10.2% I106N (ATT→AAT)  rplE ← 50S ribosomal subunit protein L5
RA 3,527,894 A→T 9.1% I476F (ATT→TTT)  igaA → inner membrane protein ‑ inhibits the Rcs signaling pathway
RA 3,530,853 A→G 8.2% D529D (GAT→GAC yhgE ← putative transport protein YhgE
RA 3,560,455:1 +G 100% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,672,390 T→C 10.5% intergenic (‑496/‑24) yhjB ← / → yhjC putative DNA‑binding transcriptional regulator YhjB/putative DNA‑binding transcriptional regulator YhjC
RA 3,750,797 C→G 30.6% intergenic (+16/+331) sgbE → / ← yiaT L‑ribulose‑5‑phosphate 4‑epimerase SgbE/outer membrane protein YiaT
RA 3,769,311 A→G 5.2% intergenic (+421/‑34) yibS → / → yibV RHS family protein YibS/PF15596 family protein YibV
MC JC 3,815,859 Δ82 bp 100% [rph] [rph]
RA 3,828,980 A→C 43.3% Q12H (CAA→CAC xanP → xanthine:H(+) symporter XanP
RA 4,045,402 G→A 6.9% A402T (GCA→ACA)  yihF → uncharacterized protein YihF
RA 4,175,680 T→A 9.5% noncoding (8/75 nt) glyT → tRNA‑Gly
RA 4,183,814 T→A 70.7% V857E (GTG→GAG)  rpoB → RNA polymerase subunit beta
JC 4,188,490 +CGT 17.0% coding (3141/4224 nt) rpoC → RNA polymerase subunit beta'
RA 4,193,487 C→T 13.8% Q30Q (CAG→CAA thiF ← ThiS adenylyltransferase
RA 4,236,912 A→G 11.4% I6V (ATT→GTT)  yjbG → capsule biosynthesis GfcC family protein YjbG
RA 4,296,060 C→T 24.3% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,503,087 Δ1 bp 14.3% coding (345/1329 nt) insG ← KpLE2 phage‑like element; IS4 putative transposase
RA 4,605,895 A→T 12.8% Q31L (CAG→CTG)  yjjZ → protein YjjZ
RA 4,608,739 G→A 9.7% V32I (GTC→ATC)  yjjG → pyrimidine nucleotidase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423775–3424234 3424541–3424239 6–767 7 [6] [6] 7 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =21 (0.600)15 (0.500) 14/200 NT 47.5% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 15 (0.500)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)49 (1.450) 36/224 NT 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 2990716 =59 (1.680)55 (1.570) 43/232 NT 48.2% pseudogene (163/490 nt) ygeF protein YgeF
?NC_000913 3130146 = NA (NA)noncoding (1/1195 nt) IS5 repeat region