breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 141 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT56.2% ‑6.4 / 38.2 68intergenic (‑41/+267)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009132,177,2340CA55.8% 17.6 / 49.9 67intergenic (‑30/+278)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009133,815,8090GC55.6% 7.2 / 39.9 27intergenic (‑41/+54)pyrE/rphorotate phosphoribosyltransferase/truncated RNase PH
*NC_000913454,9190GT54.8% 12.2 / 25.8 34intergenic (+130/‑214)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_000913454,9330TA54.5% ‑4.6 / 21.9 36intergenic (+144/‑200)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_000913915,1990AC53.8% 2.5 / 12.1 15intergenic (‑342/+153)lysO/aqpZL‑lysine exporter/water channel AqpZ
*NC_0009132,074,7070AC53.8% 6.5 / 15.0 15intergenic (+49/‑72)flu/yeeRCP4‑44 prophage; self recognizing antigen 43 (Ag43) autotransporter/CP4‑44 prophage; inner membrane protein YeeR
*NC_0009132,177,2280CG53.1% 17.4 / 67.2 67intergenic (‑24/+284)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913707,9810CG52.2% 17.1 / 37.5 31intergenic (+224/‑353)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_0009133,815,8080AC51.8% 9.3 / 31.9 27intergenic (‑40/+55)pyrE/rphorotate phosphoribosyltransferase/truncated RNase PH
*NC_0009132,177,2210TG49.3% 26.2 / 42.6 69intergenic (‑17/+291)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009132,177,2550GT49.2% 27.8 / 39.7 67intergenic (‑51/+257)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009132,470,4270CA47.4% 36.7 / 10.5 39Q433K (CAA→AAA) yfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_0009132,519,2120AC46.8% 22.9 / 26.8 48intergenic (+7/+45)yfeD/gltXputative DNA‑binding transcriptional regulator YfeD/glutamate‑‑tRNA ligase
*NC_000913707,9840CG46.7% 24.1 / 12.9 31intergenic (+227/‑350)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_000913454,8770AT46.4% 2.3 / 10.8 29intergenic (+88/‑256)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009133,815,8000CA46.4% 25.5 / 11.0 28intergenic (‑32/+63)pyrE/rphorotate phosphoribosyltransferase/truncated RNase PH
*NC_0009134,220,0160AT44.7% 31.2 / 22.9 38Y474F (TAT→TTT) aceKisocitrate dehydrogenase kinase/phosphatase
*NC_0009131,879,8460TG43.6% 40.4 / 20.6 55T35P (ACG→CCG) yeaRDUF1971 domain‑containing protein YeaR
*NC_0009133,046,0020AC43.6% 25.5 / 12.6 43intergenic (‑101/+166)ygfF/gcvPputative NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/glycine decarboxylase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4542682 =79 (0.960)6 (0.080) 6/238 5.5 8.1% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 63 (0.820)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 120780578 (0.940)5 (0.070) 5/224 5.6 7.2% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 61 (0.840)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 2996360 =NA (NA)8 (0.100)
+C
5/254 6.2 NA pseudogene (1/399 nt) ygeO putative uncharacterized protein YgeO
?NC_000913 = 2997691 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 2068941 =NA (NA)11 (0.130) 5/256 6.3 NA noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 2070273 NA (NA)intergenic (‑93/‑4) insC‑3/yoeA CP4‑44 prophage; IS2 insertion element repressor InsA/CP4‑44 prophage; TonB‑dependent receptor plug domain‑containing protein YoeA
* ? NC_000913 4498182 =NA (NA)6 (0.070) 3/254 7.3 NA noncoding (2/1331 nt) IS2 repeat region
?NC_000913 = 4499514 NA (NA)intergenic (+14/+79) insD‑6/yjgX KpLE2 phage‑like element; IS2 insertion element protein InsB/KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment