breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 18,124 C→G 27.9% G212G (GGC→GGG nhaA → Na(+):H(+) antiporter NhaA
RA 153,375 G→C 16.7% S684R (AGC→AGG htrE ← putative fimbrial usher protein HtrE
RA 238,497 G→T 19.1% I80I (ATC→ATA ykfM ← uncharacterized protein YkfM
RA 238,507 G→T 19.0% S77* (TCA→TAA)  ykfM ← uncharacterized protein YkfM
RA 238,523 G→T 17.8% L72I (CTT→ATT)  ykfM ← uncharacterized protein YkfM
RA 238,535 G→T 32.2% H68N (CAT→AAT)  ykfM ← uncharacterized protein YkfM
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
RA 473,581 A→G 7.5% K206E (AAA→GAA)  amtB → ammonia/ammonium transporter
RA 538,323 G→A 6.1% V231M (GTG→ATG)  ybbW → putative allantoin transporter
RA 638,456 C→T 6.3% A40T (GCC→ACC)  dsbG ← protein sulfenic acid reductase DsbG
RA 729,383 T→G 35.1% intergenic (+43/‑200) ybfA → / → rhsC DUF2517 domain‑containing protein YbfA/rhs element protein RhsC
RA 801,680 A→T 8.5% I308F (ATC→TTC)  ybhH → putative isomerase YbhH
RA 869,586 A→G 7.0% Y59C (TAC→TGC)  gsiB → glutathione ABC transporter periplasmic binding protein
RA 913,335 T→C 7.4% K160K (AAA→AAG hcp ← protein S‑nitrosylase
RA 969,114 T→A 7.1% M250K (ATG→AAG)  lpxK → tetraacyldisaccharide 4'‑kinase
RA 1,301,271 C→T 5.1% P30P (CCC→CCT oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,411,172 C→T 7.1% L259L (CTG→CTA ttcA ← tRNA cytosine(32) 2‑sulfurtransferase TtcA
RA 1,736,066 C→T 5.5% intergenic (+107/+55) sodB → / ← ydhP superoxide dismutase (Fe)/putative transporter YdhP
RA 1,835,143 G→A 7.7% L112L (CTG→CTA ydjZ → DedA family protein YdjZ
RA 1,957,160 Δ1 bp 6.2% coding (973/1101 nt) torY ← cytochrome c quinol dehydrogenase TorY
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
RA 1,979,450 Δ1 bp 6.4% intergenic (‑235/‑303) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 1,990,076 T→C 5.6% G132G (GGT→GGC tyrP → tyrosine:H(+) symporter
RA 2,061,004 C→T 9.1% intergenic (‑90/+12) cbl ← / ← nac DNA‑binding transcriptional activator Cbl/DNA‑binding transcriptional dual regulator Nac
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,177,245 G→T 59.1% intergenic (‑41/+267) gatY ← / ← fbaB tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
RA 2,306,168 G→A 9.1% A196V (GCA→GTA)  mqo ← malate:quinone oxidoreductase
RA 2,314,181 G→T 18.4% D232Y (GAT→TAT)  rcsD → RcsD phosphotransferase
RA 2,439,618 C→T 5.2% G168D (GGC→GAC)  yfcJ ← putative transporter YfcJ
RA 2,589,891 T→G 19.8% V766G (GTG→GGG)  acrD → multidrug efflux pump RND permease AcrD
RA 2,996,252 Δ1 bp 5.1% pseudogene (109/399 nt) ygeO ← putative uncharacterized protein YgeO
RA 3,110,522 A→G 6.9% intergenic (+81/+68) pheV → / ← yghD tRNA‑Phe/putative type II secretion system M‑type protein
RA 3,408,011 C→G 18.3% F135L (TTC→TTG panF → pantothenate:Na(+) symporter
RA 3,418,500 T→A 27.9% N37K (AAT→AAA yhdV → lipoprotein YhdV
RA 3,454,069 G→T 35.1% Q444K (CAA→AAA)  gspA ← Type II secretion system protein GspA
RA 3,560,455:1 +G 100% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,674,767 C→A 17.8% intergenic (+392/‑19) yhjD → / → yhjE putative transporter YhjD/putative transporter YhjE
RA 3,733,095 G→T 5.3% G299* (GGA→TGA)  xylG → xylose ABC transporter ATP binding subunit
RA 3,757,616 T→G 7.7% N71T (AAC→ACC)  yiaY ← L‑threonine dehydrogenase
RA 3,815,645 T→C 13.3% T42A (ACC→GCC)  pyrE ← orotate phosphoribosyltransferase
MC JC 3,815,859 Δ82 bp 100% [rph] [rph]
RA 3,828,980 A→C 45.6% Q12H (CAA→CAC xanP → xanthine:H(+) symporter XanP
RA 3,828,985 T→G 41.0% V14G (GTT→GGT)  xanP → xanthine:H(+) symporter XanP
RA 3,870,593 C→T 8.5% A380T (GCC→ACC)  dgoT ← putative D‑galactonate transporter
RA 3,883,254 T→A 6.9% D159V (GAT→GTT)  dnaA ← chromosomal replication initiator protein DnaA
RA 3,997,279 C→T 8.2% R33* (CGA→TGA)  yigB → 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase
MC JC 4,123,449 Δ990 bp 100% coding (19‑1008/1026 nt) cytR ← DNA‑binding transcriptional repressor CytR
RA 4,127,647 T→C 6.8% K83R (AAG→AGG)  yiiX ← putative lipid binding hydrolase
JC 4,186,107 (TCCGCTGGT)1→2 89.2% coding (758/4224 nt) rpoC → RNA polymerase subunit beta'
RA 4,283,228 T→G 54.2% intergenic (‑153/+25) yjcF ← / ← actP pentapeptide repeat‑containing protein YjcF/acetate/glycolate:cation symporter
RA 4,283,230 A→C 41.1% intergenic (‑155/+23) yjcF ← / ← actP pentapeptide repeat‑containing protein YjcF/acetate/glycolate:cation symporter
RA 4,296,060 C→T 31.8% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,320,073 C→T 6.8% M197I (ATG→ATA phnI ← carbon‑phosphorus lyase core complex subunit PhnI
RA 4,348,007 G→C 8.7% I246M (ATC→ATG dcuB ← anaerobic C4‑dicarboxylate transporter DcuB
RA 4,398,407 G→C 22.1% Q332H (CAG→CAC mutL → DNA mismatch repair protein MutL
RA 4,523,229 T→A 7.1% E289V (GAA→GTA)  yjhG ← KpLE2 phage‑like element; D‑xylonate dehydratase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381258 =NA (NA)6 (0.100) 6/234 NT NA pseudogene (3/417 nt) yaiX putative acyltransferase, N‑terminal fragment
?NC_000913 = 382589 NA (NA)noncoding (1330/1331 nt) IS2 repeat region
* ? NC_000913 1207790 =26 (0.450)42 (0.840) 33/204 NT 68.0% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 17 (0.340)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780526 (0.450)28 (0.560) 17/204 NT 58.7% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 17 (0.340)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)68 (1.220) 38/228 NT 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1467909 =NA (NA)3 (0.050) 3/236 NT NA pseudogene (2518/2518 nt) ydbA putative outer membrane protein, N‑terminal fragment
?NC_000913 = 1469240 NA (NA)noncoding (1/1331 nt) IS2 repeat region