mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | TF_KALE_cytR_A12_F106_I0_R1_S93_L001_R1_001.good.fq | 245,676 | 31,989,709 | 100.0% | 130.2 bases | 139 bases | 97.8% |
| errors | TF_KALE_cytR_A12_F106_I0_R1_S93_L001_R2_001.good.fq | 245,676 | 31,990,124 | 100.0% | 130.2 bases | 139 bases | 96.1% |
| errors | TF_KALE_cytR_A12_F106_I0_R1_S93_L002_R1_001.good.fq | 246,351 | 31,624,091 | 100.0% | 128.4 bases | 137 bases | 98.2% |
| errors | TF_KALE_cytR_A12_F106_I0_R1_S93_L002_R2_001.good.fq | 246,351 | 31,625,698 | 100.0% | 128.4 bases | 137 bases | 97.0% |
| errors | TF_KALE_cytR_A12_F106_I0_R1_S93_L003_R1_001.good.fq | 246,257 | 31,585,076 | 100.0% | 128.3 bases | 137 bases | 98.2% |
| errors | TF_KALE_cytR_A12_F106_I0_R1_S93_L003_R2_001.good.fq | 246,257 | 31,586,065 | 100.0% | 128.3 bases | 137 bases | 97.2% |
| errors | TF_KALE_cytR_A12_F106_I0_R1_S93_L004_R1_001.good.fq | 252,215 | 32,299,578 | 100.0% | 128.1 bases | 137 bases | 98.5% |
| errors | TF_KALE_cytR_A12_F106_I0_R1_S93_L004_R2_001.good.fq | 252,215 | 32,300,310 | 100.0% | 128.1 bases | 137 bases | 97.8% |
| total | 1,980,998 | 255,000,651 | 100.0% | 128.7 bases | 139 bases | 97.6% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 51.6 | 3.1 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 1131 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 164 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.010 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.85999 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
| Junction allow suboptimal matches | FALSE |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.05 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 21:17:34 22 Apr 2021 | 21:18:08 22 Apr 2021 | 34 seconds |
| Read alignment to reference genome | 21:18:08 22 Apr 2021 | 21:22:06 22 Apr 2021 | 3 minutes 58 seconds |
| Preprocessing alignments for candidate junction identification | 21:22:06 22 Apr 2021 | 21:22:29 22 Apr 2021 | 23 seconds |
| Preliminary analysis of coverage distribution | 21:22:29 22 Apr 2021 | 21:23:36 22 Apr 2021 | 1 minute 7 seconds |
| Identifying junction candidates | 21:23:36 22 Apr 2021 | 21:23:37 22 Apr 2021 | 1 second |
| Re-alignment to junction candidates | 21:23:37 22 Apr 2021 | 21:24:35 22 Apr 2021 | 58 seconds |
| Resolving best read alignments | 21:24:35 22 Apr 2021 | 21:25:08 22 Apr 2021 | 33 seconds |
| Creating BAM files | 21:25:08 22 Apr 2021 | 21:26:09 22 Apr 2021 | 1 minute 1 second |
| Tabulating error counts | 21:26:09 22 Apr 2021 | 21:26:31 22 Apr 2021 | 22 seconds |
| Re-calibrating base error rates | 21:26:31 22 Apr 2021 | 21:26:34 22 Apr 2021 | 3 seconds |
| Examining read alignment evidence | 21:26:34 22 Apr 2021 | 21:40:28 22 Apr 2021 | 13 minutes 54 seconds |
| Polymorphism statistics | 21:40:28 22 Apr 2021 | 21:40:29 22 Apr 2021 | 1 second |
| Output | 21:40:29 22 Apr 2021 | 21:40:38 22 Apr 2021 | 9 seconds |
| Total | 23 minutes 4 seconds | ||
