breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 18,124 C→G 34.1% G212G (GGC→GGG nhaA → Na(+):H(+) antiporter NhaA
RA 36,576 C→T 8.3% V417I (GTC→ATC)  caiC ← carnitine‑CoA ligase
RA 123,466 T→G 25.0% A150A (GCT→GCG aceE → pyruvate dehydrogenase E1 component
RA 125,336 C→T 6.2% R774C (CGC→TGC)  aceE → pyruvate dehydrogenase E1 component
RA 167,786 A→C 25.1% E101D (GAA→GAC fhuA → ferrichrome outer membrane transporter/phage receptor
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
RA 343,070 G→C 7.7% E63Q (GAA→CAA)  yahK → aldehyde reductase, NADPH‑dependent
RA 357,103 T→C 6.0% F311F (TTT→TTC codA → cytosine/isoguanine deaminase
RA 497,594 G→C 36.4% P140P (CCG→CCC adk → adenylate kinase
RA 497,610 G→C 37.5% D146H (GAC→CAC)  adk → adenylate kinase
RA 531,187 G→T 9.3% L14M (CTG→ATG)  selU ← tRNA 2‑selenouridine synthase
RA 652,898 C→G 13.8% R222G (CGT→GGT)  dpiB → sensory histidine kinase DpiB
RA 676,168 G→A 6.1% A127V (GCG→GTG)  ybeQ ← Sel1 repeat‑containing protein YbeQ
RA 729,372 A→C 40.1% intergenic (+32/‑211) ybfA → / → rhsC DUF2517 domain‑containing protein YbfA/rhs element protein RhsC
RA 739,484 A→T 5.4% N162Y (AAC→TAC)  ybgA → DUF1722 domain‑containing protein YbgA
RA 896,791 G→T 9.3% D220Y (GAT→TAT)  potH → putrescine ABC transporter membrane subunit PotH
RA 935,439 T→C 9.3% V739A (GTA→GCA)  ftsK → cell division DNA translocase FtsK
MC JC 1,299,499 Δ1,199 bp 100% insH21 insH21
RA 1,434,996 G→T 11.9% pseudogene (13/51 nt) ttcC ← putative tRNA 2‑thiocytidine (s(2)C32) synthase
RA 1,435,939 C→T 5.1% V319I (GTT→ATT)  ompN ← outer membrane porin N
RA 1,459,597 C→A 10.5% R182S (CGC→AGC)  paaH → 3‑hydroxyadipyl‑CoA dehydrogenase
RA 1,478,602 G→T 6.8% G126V (GGT→GTT)  ynbB → putative CDP‑diglyceride synthase
RA 1,657,338 A→G 5.1% D40D (GAT→GAC ynfC ← UPF0257 family lipoprotein YnfC
RA 1,690,317 G→T 8.6% D156Y (GAT→TAT)  ydgA → conserved protein YdgA
RA 1,856,087 C→T 10.6% R281H (CGC→CAC)  ydjH ← putative sugar kinase YdjH
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
RA 2,054,412 C→T 6.0% A257V (GCG→GTG)  shiA → shikimate:H(+) symporter
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,177,234 C→A 52.7% intergenic (‑30/+278) gatY ← / ← fbaB tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
RA 2,177,245 G→T 56.5% intergenic (‑41/+267) gatY ← / ← fbaB tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
RA 2,189,612 C→A 8.1% S433S (TCG→TCT yehB ← putative fimbrial usher protein YehB
RA 2,407,626 G→A 7.0% L22L (CTG→CTA alaA → glutamate‑‑pyruvate aminotransferase AlaA
RA 2,412,061 A→G 6.7% L10P (CTT→CCT)  hxpA ← hexitol phosphatase A
RA 2,496,198 Δ1 bp 6.2% coding (554/921 nt) lpxP → palmitoleoyl acyltransferase
RA 2,496,199 Δ1 bp 6.2% coding (555/921 nt) lpxP → palmitoleoyl acyltransferase
RA 2,582,860 C→T 5.5% intergenic (‑83/+43) ypfG ← / ← nudK DUF1176 domain‑containing protein YpfG/GDP‑mannose hydrolase
RA 2,592,130 A→G 6.6% N175S (AAT→AGT)  dapE → succinyl‑diaminopimelate desuccinylase
RA 2,696,334 G→C 5.8% K178N (AAG→AAC mltF → membrane‑bound lytic murein transglycosylase F
RA 2,765,525 T→C 6.2% P84P (CCA→CCG yfjM ← CP4‑57 prophage; protein YfjM
RA 2,848,129 G→A 6.0% A371A (GCC→GCT hycC ← formate hydrogenlyase subunit HycC
RA 3,016,756 C→T 7.5% P233S (CCG→TCG)  ygfK → putative oxidoreductase, Fe‑S subunit
RA 3,272,687 G→C 6.8% A22G (GCA→GGA)  garR ← tartronate semialdehyde reductase
RA 3,322,187 C→T 6.0% D107N (GAT→AAT)  secG ← Sec translocon subunit SecG
RA 3,353,707 A→T 5.9% V115E (GTG→GAG)  yhcC ← radical SAM family oxidoreductase YhcC
MC JC 3,384,721 Δ435 bp 100% coding (19‑453/471 nt) argR → DNA‑binding transcriptional dual regulator ArgR
RA 3,454,069 G→T 26.7% Q444K (CAA→AAA)  gspA ← Type II secretion system protein GspA
RA 3,523,181 C→T 6.8% A104V (GCA→GTA)  mrcA → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
RA 3,560,455:1 +G 100% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
JC 3,816,605 Δ103 bp 14.2% [yicC] [yicC]
RA 3,828,985 T→G 45.0% V14G (GTT→GGT)  xanP → xanthine:H(+) symporter XanP
RA 3,877,635 C→A 38.9% intergenic (‑170/+70) yidB ← / ← gyrB conserved protein YidB/DNA gyrase subunit B
RA 4,096,637 A→T 8.9% F271Y (TTT→TAT)  rhaB ← rhamnulokinase
RA 4,182,820 C→T 76.3% H526Y (CAC→TAC)  rpoB → RNA polymerase subunit beta
RA 4,188,573 G→T 9.2% R1075L (CGT→CTT)  rpoC → RNA polymerase subunit beta'
RA 4,190,539 G→A 7.3% intergenic (‑52/+196) sroH ← / ← thiH small RNA SroH/2‑iminoacetate synthase
RA 4,230,291 C→T 7.3% intergenic (‑149/‑63) pepE ← / → rluF peptidase E/23S rRNA pseudouridine(2604) and tRNA(Tyr) pseudouridine(35) synthase
RA 4,283,228 T→G 50.6% intergenic (‑153/+25) yjcF ← / ← actP pentapeptide repeat‑containing protein YjcF/acetate/glycolate:cation symporter
RA 4,296,060 C→T 30.0% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,405,366 C→T 5.9% T227M (ACG→ATG)  purA → adenylosuccinate synthetase
RA 4,478,951 A→T 8.5% intergenic (+62/+83) rraB → / ← yjgM ribonuclease E inhibitor protein B/putative acetyltransferase YjgM
RA 4,556,759 G→T 5.5% G36C (GGT→TGT)  ytiD → protein YtiD
RA 4,610,345 A→C 20.4% H311P (CAC→CCC)  prfC → peptide chain release factor RF3

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423769–3424234 3424532–3424238 5–764 15 [12] [13] 17 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =33 (0.650)27 (0.620) 19/202 NT 57.2% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 12 (0.270)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780535 (0.690)20 (0.460) 17/202 NT 48.7% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 12 (0.270)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 381587736 (0.710)4 (0.080) 4/232 NT 9.3% coding (673/687 nt) rph truncated RNase PH
?NC_000913 3816138 = 42 (0.840)coding (412/687 nt) rph truncated RNase PH