Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_vch_tpiA 4,604,746 C→A A186E (GCA→GAA)  bglJ → bgl operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_vch_tpiA4,604,7460CA95.2% 126.5 / ‑6.6 42A186E (GCA→GAA) bglJbgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (18/22);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 2.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTA  >  NC_000913_3_vch_tpiA/4604605‑4604887
                                                                                                                                             |                                                                                                                                             
accgacTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAACCGCAATATCAAAACGATCCGTGAACATaaa                                                                                                                                        <  1:35715/149‑1 (MQ=255)
     cTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCACAACGATCCGTGAACATAAATTTaa                                                                                                                                   >  1:495820/1‑149 (MQ=255)
        aaCAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGAAGCTTAACCGCAATATCAAGCCGATCCGTGCACATAAATTTAAtgt                                                                                                                                >  2:240540/1‑149 (MQ=255)
           aaTCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGAGTGGCTACTCAATGACACAAATTGGAGAACTTTATTAACTCATTAAAAACACGGTCCGTGAACATAAATTTAATGTGAt                                                                                                                             >  1:403300/1‑149 (MQ=255)
             tCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGt                                                                                                                           <  1:56345/149‑1 (MQ=255)
                gTGGTAGATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGa                                                                                                                        <  2:65883/149‑1 (MQ=255)
                   gTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGAACATAAATTTAATGTGCTGTAGAAAc                                                                                                                     >  2:540330/1‑149 (MQ=255)
                     aCATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTg                                                                                                                   <  1:133876/149‑1 (MQ=255)
                               aaGCCGGACGTTAAGCCCGAGGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGtt                                                                                                         <  2:102725/149‑1 (MQ=255)
                               aaGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGtt                                                                                                         <  2:663281/149‑1 (MQ=255)
                                aGCCGGACGATAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATTAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTc                                                                                                        <  1:330732/149‑1 (MQ=255)
                                    ggACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACGAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGGGTCAGTTCGGAc                                                                                                    >  1:807115/1‑149 (MQ=255)
                                    ggACGTTAAGCCCGACGGAGAGAGAAATATTGCACTGTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGAc                                                                                                    >  2:182277/1‑149 (MQ=255)
                                         ttAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAgg                                                                                               >  1:471423/1‑149 (MQ=255)
                                          tAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAggg                                                                                              <  2:157384/149‑1 (MQ=255)
                                              cccGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgttgt                                                                                          <  2:695366/149‑1 (MQ=255)
                                              cccGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTCGGCGTCACTTGTGACTCAGGgatgt                                                                                          >  2:555328/1‑149 (MQ=255)
                                               ccGACGGAGAGAGAAAGTTTGCGCTTTATGGCGCGTCGCTACACAATGACATAAATTGCCGAGCAGCTTAAACGCACTATCAAATCGCTCTGTGAACATAAATTTAATGTGATGTCGAAAGTGGTCGTCAGTTCTGACGCAGGgttgtt                                                                                         <  2:201320/149‑1 (MQ=255)
                                               ccGACGGAGAGAGAAAGATCGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgttgtt                                                                                         <  1:297780/149‑1 (MQ=255)
                                                cGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGttgttg                                                                                        <  1:491062/149‑1 (MQ=255)
                                                        agagaAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGc                                                                                                                <  1:678727/117‑1 (MQ=255)
                                                        agagaAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGc                                                                                                                >  2:678727/1‑117 (MQ=255)
                                                                   gcgcTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGCTCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGt                                                                     <  2:423017/149‑1 (MQ=255)
                                                                    cgcTGTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGtt                                                                    <  1:362991/149‑1 (MQ=255)
                                                                       tttATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGTCGTCAGTTCTGCCGCAGGGTTGTTGGAGGCCGCAGCTATTCTGGtatg                                                                 >  2:341736/1‑149 (MQ=255)
                                                                           ggTCGCGTGGCTACTCAATGACACAAAGTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTtatgtatg                                                             <  2:695618/148‑1 (MQ=255)
                                                                                  tcgCTACTCAATGACACAACATGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCAtt                                                      <  1:539774/147‑1 (MQ=255)
                                                                                      tACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTAGAAACTGGGCGTCAGTACTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGa                                                  >  2:400721/1‑149 (MQ=255)
                                                                                         tCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAAc                                               >  1:752195/1‑149 (MQ=255)
                                                                                         tCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAAc                                               >  1:243009/1‑149 (MQ=255)
                                                                                               acacAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAAtgtg                                                                                                                               <  1:256508/63‑1 (MQ=255)
                                                                                               acacAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAAtgtg                                                                                                                               >  2:256508/1‑63 (MQ=255)
                                                                                                           gAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATcc                             <  1:182277/149‑1 (MQ=255)
                                                                                                              cagcCTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTTTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTa                          <  1:145289/149‑1 (MQ=255)
                                                                                                                 cTTAAACGCAATATCAAAACGATCCGTGCACATTATTGTAGGGGGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCAGCCCTATTa                       >  1:625915/1‑149 (MQ=255)
                                                                                                                    aaaCGCAATATCAAAACGAACCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGATGGAGGCCGCAGATAGTCTGTTATCTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTCTTAATc                    >  2:711451/1‑149 (MQ=255)
                                                                                                                      aCGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGCCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTACGAAGCAAGTAACGTGTTGCAGCCCTATTCATcgc                  >  1:3789/1‑147 (MQ=255)
                                                                                                                       cGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGc                 >  1:557827/1‑149 (MQ=255)
                                                                                                                        gCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGAAATTTTTCTGTTATGTATGCGGCATTGGGAAAAAAGTAATGTGTTGCCTCCCTATTAATCCGCa                >  1:407755/1‑149 (MQ=255)
                                                                                                                         cAATCTCAAAACGATCCGTGAACGTAAATTTAATGGGATGTCGAATCTGGGCGTCAGTTCTGACGCAGGGTTGTTGGACGCCGCAGAGATTATGCTATGTATGCTGCATTGCGAAACAATTAATGTGTTGCATCCCTATTAATCCGCat               <  1:711451/149‑1 (MQ=255)
                                                                                                                                 aaaCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCgg       >  1:768427/1‑149 (MQ=255)
                                                                                                                                      aTCCGTGAACATAAATTTAATGTGATGGCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTa  <  1:696138/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                             
ACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTA  >  NC_000913_3_vch_tpiA/4604605‑4604887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑