Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_vch_tpiA 482,230 G→A P725L (CCG→CTG)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_vch_tpiA482,2300GA98.1% 159.1 / NA 53P725L (CCG→CTG) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (29/23);  minor base C (1/0);  total (30/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTG  >  NC_000913_3_vch_tpiA/482087‑482374
                                                                                                                                               |                                                                                                                                                
catAAACTTTCTACACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTa                                                                                                                                             >  2:571749/1‑149 (MQ=255)
     aCTTTCCTCACCCGACCGCGGCCGATAAAGTCGTTCACATAGCCGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTc                                                                                                                                        <  1:397592/149‑1 (MQ=255)
      cctgcttcACACGACCGCGGGCGATATAGGCGTTCACATAGCCGACGCCCCATGCAGCGCCCAGAGTGGTGTTAATGCCGTTGATAGAATCACCCAGCGCCTGCGCCTTTTCCTGGTCGATATCAATCTTAAACTGCAGTGTATCTTcc                                                                                                                                       <  2:30185/145‑1 (MQ=255)
           ttcACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGAcc                                                                                                                                  >  2:671103/1‑149 (MQ=255)
                    ccGCGGTCGATAAAGGCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACg                                                                                                                         >  1:627236/1‑149 (MQ=255)
                       cgGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCACCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAACCTTCAGGGTATCTTACAGACCGTTTTGACGTAc                                                                                                                      >  2:394513/1‑149 (MQ=255)
                            gATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCCGGGTATCTCCCAGACCCTTTATACGTACGCTgg                                                                                                                 >  1:156195/1‑149 (MQ=255)
                              tAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTc                                                                                                               <  2:759905/149‑1 (MQ=255)
                                  gTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACCCCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTAGGCTGGTAAACa                                                                                                           >  1:348295/1‑149 (MQ=255)
                                   tCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACat                                                                                                          >  1:767327/1‑149 (MQ=255)
                                         acgtAGCTGCCCCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACCCCCAGCGCCTGCGCTCGTCCCTGCTCGTTATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAgg                                                                                                    <  2:174678/146‑1 (MQ=255)
                                          caTAGCTGCCGCCCCATGCTGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTAGGCTGGTCAACATATCAggg                                                                                                   >  2:702357/1‑149 (MQ=255)
                                          caTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAggg                                                                                                   <  2:310431/149‑1 (MQ=255)
                                            tAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGt                                                                                                                                              <  2:504342/104‑1 (MQ=255)
                                            tAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGt                                                                                                                                              >  1:504342/1‑104 (MQ=255)
                                               cTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGt                                                                                                                <  1:129346/131‑1 (MQ=255)
                                               cTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGt                                                                                                                >  2:129346/1‑131 (MQ=255)
                                                      cccATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCgctgct                                                                                       >  1:436665/1‑149 (MQ=255)
                                                         aTGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATGTCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGATGCTTCACTGCTTCt                                                                                    >  2:510372/1‑149 (MQ=255)
                                                             aGCGCCTAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaa                                                                                <  1:249889/149‑1 (MQ=255)
                                                             aGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAggg                                                                                                   <  1:107223/130‑1 (MQ=255)
                                                             aGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAggg                                                                                                   >  2:107223/1‑130 (MQ=255)
                                                                  ccAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAAc                                                                           >  1:753972/1‑149 (MQ=255)
                                                                     tgttggggTTGATGTGGTTGAAAGGAACACCCAGCGCCGGCGCTTTGTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACTGTTTGGACGAACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTgg                                                                        <  2:118840/146‑1 (MQ=255)
                                                                        tggtgTCAATGTCGTTGATAGAAACACCCAGCGCCTGCGCCTCTTCCTGGTCGATCTCAATATTAAACTACATCGGATCTTCGAGACCGGTTGGACGTACGCAGGCCAACGTGTCGATGTGCTTGGATGCTGCTTCATGCAACTGGTTa                                                                     >  2:524806/1‑149 (MQ=255)
                                                                                  gTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAg                                                           >  1:723602/1‑149 (MQ=255)
                                                                                     gTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCa                                                        >  1:461891/1‑149 (MQ=255)
                                                                                      ttGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGATATCTTCCAGACCGTTTGGACGTACGCTGATCAACATATCAGGGTGCTTCCCTACTTCTCCAAGCAACTGGTTACCACCCGGAGTCAg                                                       >  2:106265/1‑149 (MQ=255)
                                                                                        gtaaGAACCACCCACGGTCGGCGTTTTTTCCGGGTCGATATTAATCTTAAACTGCAGGGTATCTTCGAGACCGTTTGGACGTACGCTGGTCCACAGATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGtt                                                     <  2:35333/146‑1 (MQ=255)
                                                                                             aaaCACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAGGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg                                                >  1:391752/1‑149 (MQ=255)
                                                                                                  cccAGCGCCTGCGCTTTTTCCTGGTAGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCGTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTCCGTGAcc                                           >  2:813295/1‑149 (MQ=255)
                                                                                                   ccAGCGCCTGCGCTTCTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCa                                          >  2:684245/1‑149 (MQ=255)
                                                                                                         ccggcgcTTTTTCCTGGTCGGTATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCa                                    <  2:688124/146‑1 (MQ=255)
                                                                                                         ccTGCGCTGTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCa                                    <  2:780836/149‑1 (MQ=255)
                                                                                                           tGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCCGCCCGTTTGGACGTACGCTGGTAAGCATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTGAGTTTTTCGTGACCAAGGCCAGc                                  >  1:375837/1‑149 (MQ=255)
                                                                                                                tttttCCTGGTCGATGTCAATCTGAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGt                             <  2:372214/149‑1 (MQ=255)
                                                                                                                tttttCCTGGTCGATATCAATCTTAAACTGCAGGGTATCCTCCAGACCGTTTTGACGTACGCTGGTCAACCTCTCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAATATTTTGTGACCAAGGCCAGCCTGGt                             <  2:426753/149‑1 (MQ=255)
                                                                                                                       tGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGCACGTCCGCTGCGGAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACCCGCCTGAGTGAGTTTTTCGTGTCCATGGCCCGCCTCCTAAATCAg                      >  2:330695/1‑149 (MQ=255)
                                                                                                                             atatCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaa                <  1:165060/149‑1 (MQ=255)
                                                                                                                             atatCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaa                <  2:229888/149‑1 (MQ=255)
                                                                                                                               atCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGGCAATCAGCtcaaagt              >  2:682045/1‑149 (MQ=255)
                                                                                                                                 cAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca            >  1:318157/1‑149 (MQ=255)
                                                                                                                                    tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                               >  1:278427/1‑111 (MQ=255)
                                                                                                                                    tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                               <  2:278427/111‑1 (MQ=255)
                                                                                                                                    tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGc                                      <  1:641215/120‑1 (MQ=255)
                                                                                                                                    tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGc                                      >  2:641215/1‑120 (MQ=255)
                                                                                                                                    tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACCCGCCTGAGTCAGTGTTTCGTGACCAAGGCCAGCCTGGTCAAGCAGCgcaaagtcaaag         >  2:294293/1‑149 (MQ=255)
                                                                                                                                     cTTAAACTGCATGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGc        <  2:436665/149‑1 (MQ=255)
                                                                                                                                      ttAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc       >  1:506966/1‑149 (MQ=255)
                                                                                                                                      ttAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc       >  1:718349/1‑149 (MQ=255)
                                                                                                                                      ttAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc       <  1:36562/149‑1 (MQ=255)
                                                                                                                                       tAAACTGCAGGGTATCGTCCACACCGTTTGGTCGTACGCTGGTCCGGCAAACAAGTTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCg      <  1:607973/149‑1 (MQ=255)
                                                                                                                                           cTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg  <  2:683443/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTG  >  NC_000913_3_vch_tpiA/482087‑482374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑